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101. | | LANFRANCO, B.; FERRARO, B.; FERNANDEZ, E.; SOARES DE LIMA, J.M. Setting effective and functional, SDG-consistent, transformational pathways for agro-food chains using a flexible multi-objective, stakeholder-participatory backcasting approach. In: 23rd ICABR (Intenational Consortium on Applied Bioeconomy Research) Conference,4-7 June 2019, Ravello, Italy. [Trabajo en Congreso + Poster + Presentación Oral].Biblioteca(s): INIA La Estanzuela. |
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102. | | BALDI, F.; BANCHERO, G.; MIERES, J.; LA MANNA, A.; FERNANDEZ, E.; FORMOSO, F.; MONTOSSI, F. Suplementación en invernada intensiva: hasta donde hemos llegado? Revista INIA, 2008, no. 15, p. 2-7Biblioteca(s): INIA La Estanzuela; INIA Las Brujas; INIA Tacuarembó. |
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105. | | LA MANNA, A.; FERNANDEZ, E.; MIERES, J.; BANCHERO, G.; VAZ MARTINS, D. Suplementación infrecuente: ¿Es posible trabajar menos y producir lo mismo? ln: INIA LA ESTANZUELA. Jornada de Invernada Intensiva, julio, La Estanzuela, Colonia, Uruguay, 2010. Producción de carne desde una invernada de precisión. La Estanzuela, Colonia (Uruguay): INIA, 2010. p. 31-34 (INIA Serie Actividades de Difusión ; 609)Biblioteca(s): INIA Tacuarembó. |
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108. | | LIBOREIRO, M.; FLORES, C.; ARMENDANO, J.; SCHILD, C.; FERNÁNDEZ, E.; ODRIOZOLA, E.; MORRELL, E.; CANTÓN, G. Spontaneous outbreak of Pascalia glauca poisoning in sheep in Argentina. (Case Report). Brazilian Journal of Veterinary Pathology, 2021, Volume 14, Issue 2, Pages 99-101. OPEN ACCESS. doi: https://doi.org/10.24070/bjvp.1983-0246.v14i2p99-101 Article history: Submitted 29 December 2020; Accepted 22 April 2021.
Corresponding author: Cantón, G.; Instituto Nacional de Tecnología Agropecuaria (INTA), Ruta Nacional 226 km. 73.5, Balcarce, Argentina; email:canton.german@inta.gob.ar...Biblioteca(s): INIA Las Brujas. |
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112. | | VAZ MARTINS, D.; LA MANNA, A.; MESSA, A.; FERNANDEZ, E.; DEL CAMPO, M.; BRITO, G. Resultados y discusión-componente bovino. Performance animal y características carniceras in vivo de novillos hereford sometidos a distintas estrategias de alimentación en la última etapa del proceso de engorde In: MONTOSSI, F.; SAÑUDO, C. (Eds.). Cooperación Hispano-Uruguaya. Diferenciación y valorización de la carne ovina y bovina del Uruguay en Europa: Influencia de Sistemas de Producción sobre Bienestar Animal, Atributos Sensoriales, Aceptabilidad y Percepción de Consumidores y Salud Humana Montevideo (Uruguay): INIA, 2007. p. 37-42 (INIA Serie Técnica ; 168)Biblioteca(s): INIA Las Brujas; INIA Tacuarembó. |
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113. | | SOARES DE LIMA, J.M.; FERNANDEZ, E.; FERRARO, B.; ZORRILLA DE SAN MARTÍN, G.; LANFRANCO, B. Riego por aspersión en rotaciones arroz-soja-pasturas. In: Terra, J. A.; Martínez, S.; Saravia, H.; Mesones, B. (Eds.) Arroz 2021. Montevideo (UY): INIA, 2022. p. 33-36. (INIA Serie Técnica; 262)Biblioteca(s): INIA Treinta y Tres. |
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114. | | QUINCKE, A.; CIGANDA, V.; SAWCHIK, J.; FERNANDEZ, E.; HIRIGOYEN, D.; SOTELO, D.; RESTAINO, E.; LAPETINA, J. Rotaciones cultivos - pasturas INIA La Estanzuela. Aprendiendo del experimento más antiguo de Latinoamérica. Informe especial. Revista INIA Uruguay, 2019, no. 59, p.46-60. (Revista INIA; 59). Entrevistas a: Ing. Agr. Fernando García Préchac (Director General de Recursos Naturales del MGAP), Ing. Agr. Roberto Díaz (Ex Director de INIA La Estanzuela, Ex Investigador en Manejo de Suelos), Ing. Agr. Alejandro Morón (Ex Responsable...Biblioteca(s): INIA Las Brujas. |
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118. | | COZZOLINO, D.; RESTAINO, E.; LA MANNA, A.; FERNANDEZ, E.; FASSIO, A. Usefulness of near infrared reflectance (NIR) spectroscopy and chemometrics to discriminate between fishmeal, meat meal and soya meal samples. Ciencia e Investigacion Agraria, v. 36, n. 2, p. 209-214, 2009.Biblioteca(s): INIA La Estanzuela. |
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120. | | LÓPEZ RIBADA, M.; PERNA, R.; FERNÁNDEZ, E.; ORNSTEIN, A.; ZURITA, M.; TROPEANO, M.; BRYNKIER, J.; PUENTE, J. Anestesia intravenosa en rumiantes para práctica rural ln: Jornadas Uruguayas de Buiatría, 24., 1996, Paysandú, Uruguay Trabajos presentados. Paysandú (Uruguay): Centro Médico Veterinario de Paysandú, 1996. P.3, p. 1-3Biblioteca(s): INIA Tacuarembó; INIA Treinta y Tres. |
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Registros recuperados : 164 | |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha actual : |
22/09/2022 |
Actualizado : |
22/09/2022 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Circulación / Nivel : |
Internacional - -- |
Autor : |
UMPIÉRREZ , A.; ERNST, E.; CARDOZO, A.; TORRES, A.; FERNÁNDEZ, M.; FRAGA, M.; VIGNOLI, R.; BADO, I.; VIDAL, R.; ZUNINO, P. |
Afiliación : |
ANA UMPIÉRREZ, Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay; DÉBORAH ERNST, Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay.; ANDREA CARDOZO, Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay.; ALEXIA TORRES, Programa de Microbiología y Micología, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Santiago, Chile.; MAGALÍ FERNÁNDEZ, Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay.; MARTIN FRAGA COTELO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RAFAEL VIGNOLI, Departamento de Bacteriología y Virología, Instituto de Higiene, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay.; INÉS BADO, Departamento de Bacteriología y Virología, Instituto de Higiene, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay.; ROBERTO VIDAL, Programa de Microbiología y Micología, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Santiago, Chile; Instituto Milenio de Inmunología e Inmunoterapia, Facultad de Medicina, Universidad de Chile, Santiago, Chile.; PABLO ZUNINO, Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay. |
Título : |
Non-O157 Shiga toxin-producing Escherichia coli with potential harmful profiles to humans are isolated from the faeces of calves in Uruguay. |
Fecha de publicación : |
2022 |
Fuente / Imprenta : |
Austral Journal of Veterinary Sciences, 2022, Vol. 54 Issue 2, p.45-53. doi: https://doi.org/10.4067/S0719-81322022000200045 |
Descripción física : |
SSN 0719-8132 (version on-line)
ISSN 0719-8000 (version print) |
ISSN : |
0719-8132 (print); e-ISSN 0719-8000 (electronic) |
DOI : |
10.4067/S0719-81322022000200045 |
Idioma : |
Inglés |
Notas : |
Article history: Received 12 October 2021; Accepted 30 December 2021; Published 09 May 2022.
Corresponding author: Ana Umpiérrez; Avenida Italia 3318, CP 11600, Montevideo, Uruguay; aumpierrez@iibce.edu.uy |
Contenido : |
ABSTRACT.- Shiga toxin-producing Escherichia coli (STEC) infections are responsible for acute illnesses and deaths in humans. Cattle and humans are exposed to STEC through faeces and contaminated food and water. The big six and O157 STEC serogroups are important food and water-borne human pathogens. Additionally, Stx1a, Stx2a and Stx2c subtypes are highly associated with the haemolytic uremic syndrome. This study aimed to determine Shiga toxin-subtypes, the presence of antigen 43 families, the genotypic and phenotypic antimicrobial susceptibility profiles, O-serogrouping, phylotypes and phylogenetic relatedness of STEC of calf origin. Sixteen STEC isolates from calf origin were analysed. PCR was performed to determine Stx subtypes, serogroups, the presence of ag43 I and IIand phylotypes. The antimicrobial profile was evaluated and the presence of PMQR and fosfomycin genes was determined by PCR. The clonal relatedness of STEC was studied by PFGE. The genotypes stx1a+c,stx1a+, stx1a+/stx2e+, stx1a+c/stx2e and stx2awere detected. Ag43 II was the most prevalent among subfamilies. STEC isolates were serotyped as O103 (n=5) and O111 (n=6). Fifty per cent of the isolates were classified as B1 phylogroup, 4/16 as E, 1/16 as C, and 1/16 as F. Non-O157 STEC isolates showed a high level of diversity, independent of the geographical and farm-origin. Isolates were resistant to ampicillin, ciprofloxacin, gentamicin, and fosfomycin-trometamol. The gene fosA7 was detected in 1 isolate. The virulence profiles, including Shiga toxin-subtypes and serogroups, denote the potential harm of non-O157 STEC isolates to humans. We also confirmed that circulating non-O157 STEC from cattle present genetic heterogeneity and are susceptible to antibiotics. MenosABSTRACT.- Shiga toxin-producing Escherichia coli (STEC) infections are responsible for acute illnesses and deaths in humans. Cattle and humans are exposed to STEC through faeces and contaminated food and water. The big six and O157 STEC serogroups are important food and water-borne human pathogens. Additionally, Stx1a, Stx2a and Stx2c subtypes are highly associated with the haemolytic uremic syndrome. This study aimed to determine Shiga toxin-subtypes, the presence of antigen 43 families, the genotypic and phenotypic antimicrobial susceptibility profiles, O-serogrouping, phylotypes and phylogenetic relatedness of STEC of calf origin. Sixteen STEC isolates from calf origin were analysed. PCR was performed to determine Stx subtypes, serogroups, the presence of ag43 I and IIand phylotypes. The antimicrobial profile was evaluated and the presence of PMQR and fosfomycin genes was determined by PCR. The clonal relatedness of STEC was studied by PFGE. The genotypes stx1a+c,stx1a+, stx1a+/stx2e+, stx1a+c/stx2e and stx2awere detected. Ag43 II was the most prevalent among subfamilies. STEC isolates were serotyped as O103 (n=5) and O111 (n=6). Fifty per cent of the isolates were classified as B1 phylogroup, 4/16 as E, 1/16 as C, and 1/16 as F. Non-O157 STEC isolates showed a high level of diversity, independent of the geographical and farm-origin. Isolates were resistant to ampicillin, ciprofloxacin, gentamicin, and fosfomycin-trometamol. The gene fosA7 was detected in 1 isolate. The ... Presentar Todo |
Palabras claves : |
Antimicrobial resistance; Non-O157 STEC; PLATAFORMA EN SALUD ANIMAL; Shiga toxin subtypes. |
Asunto categoría : |
L01 Ganadería |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/16768/1/10.4067-s0719-81322022000200045.pdf
|
Marc : |
LEADER 03084naa a2200325 a 4500 001 1063578 005 2022-09-22 008 2022 bl uuuu u00u1 u #d 022 $a0719-8132 (print); e-ISSN 0719-8000 (electronic) 024 7 $a10.4067/S0719-81322022000200045$2DOI 100 1 $aUMPIÉRREZ , A. 245 $aNon-O157 Shiga toxin-producing Escherichia coli with potential harmful profiles to humans are isolated from the faeces of calves in Uruguay.$h[electronic resource] 260 $c2022 300 $cSSN 0719-8132 (version on-line) ISSN 0719-8000 (version print) 500 $aArticle history: Received 12 October 2021; Accepted 30 December 2021; Published 09 May 2022. Corresponding author: Ana Umpiérrez; Avenida Italia 3318, CP 11600, Montevideo, Uruguay; aumpierrez@iibce.edu.uy 520 $aABSTRACT.- Shiga toxin-producing Escherichia coli (STEC) infections are responsible for acute illnesses and deaths in humans. Cattle and humans are exposed to STEC through faeces and contaminated food and water. The big six and O157 STEC serogroups are important food and water-borne human pathogens. Additionally, Stx1a, Stx2a and Stx2c subtypes are highly associated with the haemolytic uremic syndrome. This study aimed to determine Shiga toxin-subtypes, the presence of antigen 43 families, the genotypic and phenotypic antimicrobial susceptibility profiles, O-serogrouping, phylotypes and phylogenetic relatedness of STEC of calf origin. Sixteen STEC isolates from calf origin were analysed. PCR was performed to determine Stx subtypes, serogroups, the presence of ag43 I and IIand phylotypes. The antimicrobial profile was evaluated and the presence of PMQR and fosfomycin genes was determined by PCR. The clonal relatedness of STEC was studied by PFGE. The genotypes stx1a+c,stx1a+, stx1a+/stx2e+, stx1a+c/stx2e and stx2awere detected. Ag43 II was the most prevalent among subfamilies. STEC isolates were serotyped as O103 (n=5) and O111 (n=6). Fifty per cent of the isolates were classified as B1 phylogroup, 4/16 as E, 1/16 as C, and 1/16 as F. Non-O157 STEC isolates showed a high level of diversity, independent of the geographical and farm-origin. Isolates were resistant to ampicillin, ciprofloxacin, gentamicin, and fosfomycin-trometamol. The gene fosA7 was detected in 1 isolate. The virulence profiles, including Shiga toxin-subtypes and serogroups, denote the potential harm of non-O157 STEC isolates to humans. We also confirmed that circulating non-O157 STEC from cattle present genetic heterogeneity and are susceptible to antibiotics. 653 $aAntimicrobial resistance 653 $aNon-O157 STEC 653 $aPLATAFORMA EN SALUD ANIMAL 653 $aShiga toxin subtypes 700 1 $aERNST, E. 700 1 $aCARDOZO, A. 700 1 $aTORRES, A. 700 1 $aFERNÁNDEZ, M. 700 1 $aFRAGA, M. 700 1 $aVIGNOLI, R. 700 1 $aBADO, I. 700 1 $aVIDAL, R. 700 1 $aZUNINO, P. 773 $tAustral Journal of Veterinary Sciences, 2022, Vol. 54 Issue 2, p.45-53. doi: https://doi.org/10.4067/S0719-81322022000200045
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