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| 28. |  | CRISCI, C.; VILARO, F. Cosecha de papa In: CRISCI, C.; VILARO, F. Aportes tecnológicos para el cultivo de papa. Montevideo (Uruguay): INIA, 1993. p17-24 (INIA Boletin de Divulgación; 32)| Biblioteca(s): INIA La Estanzuela; INIA Las Brujas; INIA Tacuarembó. |
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| 34. | | VILARÓ, F.; SUÁREZ, C. Evaluación de variedades de cebolla en la zona sur. In: INIA (Instituto Nacional de Investigación Agropecuaria); PROGRAMA HORTICULTURA. Resultados experimentales en cebolla 1993-1994. Reunión técnica. Canelones (UY): INIA Las Brujas, 1994. p. 36-39 (INIA Serie Actividades de Difusión ; 6)| Biblioteca(s): INIA La Estanzuela; INIA Las Brujas; INIA Tacuarembó. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
02/01/2017 |
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Actualizado : |
15/10/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
CASTILLO, A.; GAIERO, P.; LÓPEZ CARRO, B.; VILARO, F. |
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Afiliación : |
ALICIA MARIA CASTILLO SALLE, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; P. GAIERO, Universidad de la República (UdelaR)/ Facultad de Agronomía; B. LÓPEZ CARRO, SECIF-IIBCE (Instituto de Investigaciones de Ciencias Biológicas "Clemente Estable"); FRANCISCO LUIS VILARO PAREJA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Gametic embryogenic response in wild diploid Solanum species and its implications for genome sequencing projects and breeding. |
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Fecha de publicación : |
2016 |
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Fuente / Imprenta : |
Plant Tissue Culture and Biotechnology, 2016, v. 26 (2): 159-173. |
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DOI : |
10.3329/ptcb.v26i2.30566 |
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Idioma : |
Inglés |
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Contenido : |
ABSTRACT.
The anther culture response in Solanum commersonii (2n = 2x = 24, 1EBN) and S. chacoense (2n = 2x = 24, 2EBN), two wild potato germplasm resources was studied to obtain haploid plants. Three accessions from each of the two species and 3200 anthers from each genotype were cultured. Authors assessed different culture media; ascorbic acid, L?cysteine and silver nitrate (AgNO3) were included to prevent browning of anther cultures. Addition of AgNO3, was effective to induce embryogenesis. The clones from S. commersonii showed different embryogenic response to androgenesis. However, the three accessions from S. chacoense did not induce any embryo in the same conditions. Ploidy level of the regenerated clones was estimated by flow cytometry and confirmed by chromosome counts. This is the first report of haploid plants obtained from anther culture in S. commersonii, with important implications in sequencing efforts and potato breeding. |
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Palabras claves : |
ANTHER CULTURE; DOUBLE HAPLOID; FLOW CYTOMETRY; HAPLOID. |
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Thesagro : |
GERMOPLASMA; PAPA; SOLANUM COMMERSONII. |
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Asunto categoría : |
-- |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/13501/1/30566-Article-Text-109802-1-10-20161210.pdf
https://www.banglajol.info/index.php/PTCB/article/view/30566
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Marc : |
LEADER 01724naa a2200253 a 4500
001 1056407
005 2019-10-15
008 2016 bl uuuu u00u1 u #d
024 7 $a10.3329/ptcb.v26i2.30566$2DOI
100 1 $aCASTILLO, A.
245 $aGametic embryogenic response in wild diploid Solanum species and its implications for genome sequencing projects and breeding.$h[electronic resource]
260 $c2016
520 $aABSTRACT. The anther culture response in Solanum commersonii (2n = 2x = 24, 1EBN) and S. chacoense (2n = 2x = 24, 2EBN), two wild potato germplasm resources was studied to obtain haploid plants. Three accessions from each of the two species and 3200 anthers from each genotype were cultured. Authors assessed different culture media; ascorbic acid, L?cysteine and silver nitrate (AgNO3) were included to prevent browning of anther cultures. Addition of AgNO3, was effective to induce embryogenesis. The clones from S. commersonii showed different embryogenic response to androgenesis. However, the three accessions from S. chacoense did not induce any embryo in the same conditions. Ploidy level of the regenerated clones was estimated by flow cytometry and confirmed by chromosome counts. This is the first report of haploid plants obtained from anther culture in S. commersonii, with important implications in sequencing efforts and potato breeding.
650 $aGERMOPLASMA
650 $aPAPA
650 $aSOLANUM COMMERSONII
653 $aANTHER CULTURE
653 $aDOUBLE HAPLOID
653 $aFLOW CYTOMETRY
653 $aHAPLOID
700 1 $aGAIERO, P.
700 1 $aLÓPEZ CARRO, B.
700 1 $aVILARO, F.
773 $tPlant Tissue Culture and Biotechnology, 2016$gv. 26 (2): 159-173.
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