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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
05/12/2016 |
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Actualizado : |
09/10/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
VERDES, J. M.; MÁRQUEZ, M.; CALLIARI, A.; BATES, D.; MORAÑA, J. A.; GIMENO, E. J.; ODRIOZOLA, E.; GIANNITTI, F.; GUERRERO, F.; FIDALGO, L. E.; PUMAROLA, M. |
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Afiliación : |
JOSÉ MANUEL VERDES, Universidad de la República (UdelaR)/ Facultad de Veterinaria; MEC/ IIBCE (Instituto de Investigaciones de Ciencias Biológicas "Clemente Estable"); MERCEDES MÁRQUEZ, Universidad de Santiago de Compostela (USC); ALDO CALLIARI, Universidad de la República (UdelaR)/ Facultad de Veterinaria; MEC/ IIBCE (Instituto de Investigaciones de Ciencias Biológicas "Clemente Estable"); DANIEL BATES, Universidad de la República (UdelaR)/ Facultad de Veterinaria; JOSÉ ANTONIO MORAÑA, Universidad de la República (UdelaR)/ Facultad de Veterinaria; EDUARDO JUAN GIMENO, Universidad Nacional de la Plata (UNLP); ERNESTO ODRIOZOLA, INTA (Instituto Nacional de Tecnología Agropecuaria)- Balcarce; FEDERICO GIANNITTI, University of Minnesota, Saint Paul, MN, USA; INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FLORENTINA GUERRERO, Universidad de Santiago de Compostela (USC); LUIS EUSEBIO FIDALGO, Universidad de Santiago de Compostela (USC); MARTÍ PUMAROLA, Universitat Autònoma de Barcelona. |
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Título : |
A novel pathogenic mechanism for cerebellar lesions produced by Solanum bonariense in cattle. (Full Scientific Report) |
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Fecha de publicación : |
2015 |
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Fuente / Imprenta : |
Journal of Veterinary Diagnostic Investigation, 2015, v.27 (3): 278-286. |
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DOI : |
10.1177/1040638715582048 |
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Idioma : |
Inglés |
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Notas : |
Article history: First Published April 21, 2015. |
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Contenido : |
ABSTRACT.
Intoxication with Solanum bonariense in cattle causes cerebellar cortical degeneration with perikaryal vacuolation, axonal swelling, and death primarily of Purkinje cells, with accumulation of electron-dense residual storage bodies in membrane-bound vesicles. The pathogenesis of this disease is not fully understood. Previously, we proposed that inhibition of protein synthesis in Purkinje cells among other altered metabolic pathways could lead to cytoskeletal alterations, subsequently altering cell-specific axonal transport. In the present study, immunohistochemical and histochemical methods were used to identify neuronal cytoskeletal alterations and axonal loss, demyelination, and astrogliosis in the cerebellum of intoxicated bovines. Samples of cerebellum from 3 natural and 4 experimental cases and 2 control bovines were studied. Immunoreactivity against neurofilament (NF)-200KDa confirmed marked loss of Purkinje neurons, and phospho-NF protein, ?-tubulin, and affinity reaction against phalloidin revealed an altered perikaryal distribution of neuronal cytoskeletal proteins in the remaining Purkinje cells in intoxicated cattle. Reactive astrogliosis in every layer of the cerebellar cortex was also observed with anti?glial fibrillary acidic protein immunohistochemistry. In affected cattle, demyelination and axonal loss in the cerebellar white matter, as well as basket cell loss were demonstrated with Klüver?Barrera and Bielschowsky stains, respectively. Based on these results, we propose that neuronal cytoskeletal alterations with subsequent interference of the axonal transport in Purkinje cells may play a relevant role in the pathogenesis of this neurodegenerative disorder, and also that demyelination and axonal loss in the cerebellar white matter, as well as astrogliosis in the gray matter, likely occur secondarily to Purkinje cell degeneration and death.
© 2015 The Author(s). MenosABSTRACT.
Intoxication with Solanum bonariense in cattle causes cerebellar cortical degeneration with perikaryal vacuolation, axonal swelling, and death primarily of Purkinje cells, with accumulation of electron-dense residual storage bodies in membrane-bound vesicles. The pathogenesis of this disease is not fully understood. Previously, we proposed that inhibition of protein synthesis in Purkinje cells among other altered metabolic pathways could lead to cytoskeletal alterations, subsequently altering cell-specific axonal transport. In the present study, immunohistochemical and histochemical methods were used to identify neuronal cytoskeletal alterations and axonal loss, demyelination, and astrogliosis in the cerebellum of intoxicated bovines. Samples of cerebellum from 3 natural and 4 experimental cases and 2 control bovines were studied. Immunoreactivity against neurofilament (NF)-200KDa confirmed marked loss of Purkinje neurons, and phospho-NF protein, ?-tubulin, and affinity reaction against phalloidin revealed an altered perikaryal distribution of neuronal cytoskeletal proteins in the remaining Purkinje cells in intoxicated cattle. Reactive astrogliosis in every layer of the cerebellar cortex was also observed with anti?glial fibrillary acidic protein immunohistochemistry. In affected cattle, demyelination and axonal loss in the cerebellar white matter, as well as basket cell loss were demonstrated with Klüver?Barrera and Bielschowsky stains, respectively. Based on th... Presentar Todo |
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Palabras claves : |
BOVINE; CEREBELLAR DEGENERATION; CYTOSKELETAL DYSFUNCTION; PURKINJE CELLS; SOLANUM BONARIENSE. |
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Thesagro : |
BOVINOS. |
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Asunto categoría : |
L01 Ganadería |
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Marc : |
LEADER 02961naa a2200337 a 4500
001 1056202
005 2019-10-09
008 2015 bl uuuu u00u1 u #d
024 7 $a10.1177/1040638715582048$2DOI
100 1 $aVERDES, J. M.
245 $aA novel pathogenic mechanism for cerebellar lesions produced by Solanum bonariense in cattle. (Full Scientific Report)$h[electronic resource]
260 $c2015
500 $aArticle history: First Published April 21, 2015.
520 $aABSTRACT. Intoxication with Solanum bonariense in cattle causes cerebellar cortical degeneration with perikaryal vacuolation, axonal swelling, and death primarily of Purkinje cells, with accumulation of electron-dense residual storage bodies in membrane-bound vesicles. The pathogenesis of this disease is not fully understood. Previously, we proposed that inhibition of protein synthesis in Purkinje cells among other altered metabolic pathways could lead to cytoskeletal alterations, subsequently altering cell-specific axonal transport. In the present study, immunohistochemical and histochemical methods were used to identify neuronal cytoskeletal alterations and axonal loss, demyelination, and astrogliosis in the cerebellum of intoxicated bovines. Samples of cerebellum from 3 natural and 4 experimental cases and 2 control bovines were studied. Immunoreactivity against neurofilament (NF)-200KDa confirmed marked loss of Purkinje neurons, and phospho-NF protein, ?-tubulin, and affinity reaction against phalloidin revealed an altered perikaryal distribution of neuronal cytoskeletal proteins in the remaining Purkinje cells in intoxicated cattle. Reactive astrogliosis in every layer of the cerebellar cortex was also observed with anti?glial fibrillary acidic protein immunohistochemistry. In affected cattle, demyelination and axonal loss in the cerebellar white matter, as well as basket cell loss were demonstrated with Klüver?Barrera and Bielschowsky stains, respectively. Based on these results, we propose that neuronal cytoskeletal alterations with subsequent interference of the axonal transport in Purkinje cells may play a relevant role in the pathogenesis of this neurodegenerative disorder, and also that demyelination and axonal loss in the cerebellar white matter, as well as astrogliosis in the gray matter, likely occur secondarily to Purkinje cell degeneration and death. © 2015 The Author(s).
650 $aBOVINOS
653 $aBOVINE
653 $aCEREBELLAR DEGENERATION
653 $aCYTOSKELETAL DYSFUNCTION
653 $aPURKINJE CELLS
653 $aSOLANUM BONARIENSE
700 1 $aMÁRQUEZ, M.
700 1 $aCALLIARI, A.
700 1 $aBATES, D.
700 1 $aMORAÑA, J. A.
700 1 $aGIMENO, E. J.
700 1 $aODRIOZOLA, E.
700 1 $aGIANNITTI, F.
700 1 $aGUERRERO, F.
700 1 $aFIDALGO, L. E.
700 1 $aPUMAROLA, M.
773 $tJournal of Veterinary Diagnostic Investigation, 2015$gv.27 (3): 278-286.
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INIA Las Brujas (LB) |
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| Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha actual : |
10/09/2014 |
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Actualizado : |
04/10/2019 |
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Tipo de producción científica : |
Capítulo en Libro Técnico-Científico |
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Autor : |
UMPIÉRREZ M.; GARMENDIA G.; CABRERA M.; PEREYRA, S.; VERO, S. |
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Afiliación : |
SILVIA ANTONIA PEREYRA CORREA, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. |
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Título : |
Diversity of pathogen populations causing Fusarium head blight of wheat in Uruguay. |
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Fecha de publicación : |
2013 |
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Fuente / Imprenta : |
In ALCONADA MAGLIANO, T.M.; CHULZE, S.N. (Ed.). Fusarium Head Blight in Latin America. Dordrecht, NL: Springer, 2013. p. 31-44, 2013 |
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ISBN : |
978-94-007-7091-1 |
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DOI : |
10.1007/978-94-007-7091-1_3 |
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Idioma : |
Inglés |
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Notas : |
Article history: First Online: 01 August 2013. |
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Contenido : |
ABSTRACT.
Members of the Fusarium graminearum species complex (FGSC) are the primary pathogens causing Fusarium head blight (FHB), Fusarium graminearum is the main pathogen of FHB and can produce various mycotoxins in wheat, in particular type B trichothecenes. This review presents information on the Fusarium species and chemotypes diversity associated to FHB in wheat in Uruguay. In surveys performed in 2001 and 2002, Fusarium graminearum was the most frequently isolated species (76 %), while other non FGSC species were also identified. Among species from FGSC Fusarium graminearum sensu stricto was the most frequently isolated (97 %), while F. cortaderiae and F. austroamericanum were also identified. The predominant chemotype was 15ADON (95 %), followed by 3ADON (3 %) and NIV (2 %). Isolates identified as F. graminearum sensu stricto were characterized according to the levels of DON production, the aggressiveness on different wheat cultivars, the ability to form perithecia on wheat straw. The sensitiveness to the fungicide tebuconazole of FGSC isolates was evaluated. Most isolates showed high sensitivity levels, but a few resisted higher levels of fungicide. Lower sensitivity seemed to be tied to species since F. cortaderiae isolates showed the highest MIC values. |
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Palabras claves : |
AGRESSIVENESS; FUSARIUM ASIATICUM; FUSARIUM AUSTROAMERICANUM; FUSARIUM BRASILICUM; FUSARIUM CORTADERIAE; FUSARIUM GRAMINEARUM; FUSARIUM GRAMINEARUM SPECIES COMPLEX; MYCOTOXINS; TRITCUM. |
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Thesagro : |
CULTIVOS DE INVIERNO; FITOPATOLOGÍA. |
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Asunto categoría : |
H20 Enfermedades de las plantas |
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Marc : |
LEADER 02390naa a2200337 a 4500
001 1050097
005 2019-10-04
008 2013 bl uuuu u00u1 u #d
020 $a978-94-007-7091-1
024 7 $a10.1007/978-94-007-7091-1_3$2DOI
100 1 $aUMPIÉRREZ M.
245 $aDiversity of pathogen populations causing Fusarium head blight of wheat in Uruguay.$h[electronic resource]
260 $c2013
500 $aArticle history: First Online: 01 August 2013.
520 $aABSTRACT. Members of the Fusarium graminearum species complex (FGSC) are the primary pathogens causing Fusarium head blight (FHB), Fusarium graminearum is the main pathogen of FHB and can produce various mycotoxins in wheat, in particular type B trichothecenes. This review presents information on the Fusarium species and chemotypes diversity associated to FHB in wheat in Uruguay. In surveys performed in 2001 and 2002, Fusarium graminearum was the most frequently isolated species (76 %), while other non FGSC species were also identified. Among species from FGSC Fusarium graminearum sensu stricto was the most frequently isolated (97 %), while F. cortaderiae and F. austroamericanum were also identified. The predominant chemotype was 15ADON (95 %), followed by 3ADON (3 %) and NIV (2 %). Isolates identified as F. graminearum sensu stricto were characterized according to the levels of DON production, the aggressiveness on different wheat cultivars, the ability to form perithecia on wheat straw. The sensitiveness to the fungicide tebuconazole of FGSC isolates was evaluated. Most isolates showed high sensitivity levels, but a few resisted higher levels of fungicide. Lower sensitivity seemed to be tied to species since F. cortaderiae isolates showed the highest MIC values.
650 $aCULTIVOS DE INVIERNO
650 $aFITOPATOLOGÍA
653 $aAGRESSIVENESS
653 $aFUSARIUM ASIATICUM
653 $aFUSARIUM AUSTROAMERICANUM
653 $aFUSARIUM BRASILICUM
653 $aFUSARIUM CORTADERIAE
653 $aFUSARIUM GRAMINEARUM
653 $aFUSARIUM GRAMINEARUM SPECIES COMPLEX
653 $aMYCOTOXINS
653 $aTRITCUM
700 1 $aGARMENDIA G.
700 1 $aCABRERA M.
700 1 $aPEREYRA, S.
700 1 $aVERO, S.
773 $tIn ALCONADA MAGLIANO, T.M.; CHULZE, S.N. (Ed.). Fusarium Head Blight in Latin America. Dordrecht, NL: Springer, 2013. p. 31-44, 2013
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