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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
23/02/2021 |
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Actualizado : |
23/02/2021 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
QUEZADA, M.; AMADEU, R. R.; VIGNALE, B.; CABRERA, D.; PRITSCH, C.; GARCIA, A. A. F. |
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Afiliación : |
MARIANELLA FERNANDA QUEZADA MACCHIAVELLO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay.; RODRIGO RAMPAZO AMADEU, Laboratório de Genética Estatística, Departamento de Genética, Escola Superior de Agricultura "Luiz de Queiroz", Universidade de São Paulo, Piracicaba, Brazil; BEATRIZ VIGNALE, Mejoramiento Genético, Departamento de Producción Vegetal, Estación Experimental de la Facultad de Agronomía, Universidad de la República, Salto, Uruguay; CARLOS DANILO CABRERA BOLOGNA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CLARA PRITSCH, Laboratorio de Biotecnología, Departamento de Biología Vegetal, Facultad de Agronomía, Universidad de la República, Montevideo, Uruguay; ANTONIO AUGUSTO FRANCO GARCIA, Laboratório de Genética Estatística, Departamento de Genética, Escola Superior de Agricultura “Luiz de Queiroz”, Universidade de São Paulo, Piracicaba, Brazil. |
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Título : |
Construction of a high-density genetic map of Acca sellowiana (Berg.) Burret, an outcrossing species, based on two connected mapping populations. |
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Complemento del título : |
Original Research Article. |
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Fecha de publicación : |
2021 |
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Fuente / Imprenta : |
Frontiers in Plant Science, 23 February 2021, Volume 12, Article number 626811. OPEN ACCESS. Doi: https://doi.org/10.3389/fpls.2021.626811 |
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DOI : |
10.3389/fpls.2021.626811 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: 06 November 2020; Accepted: 12 January 2021; Published: 23 February 2021.
Edited by: Maria Luisa Badenes, Instituto Valenciano de Investigaciones Agrarias, Spain.
Reviewed by: Shouvik Das, Indian Agricultural Research Institute (ICAR), India; Jonathan Elias Maldonado, Pontificia Universidad Católica de Chile, Chile.
Correspondence: Antonio Augusto Franco Garcia - augusto.garcia@usp.br
Specialty section: This article was submitted to Plant Breeding, a section of the journal Frontiers in Plant Science.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY).
This article is part of the research topic: Breeding Innovations in Underutilized Temperate Fruit Trees:
https://www.frontiersin.org/research-topics/14915/breeding-innovations-in-underutilized-temperate-fruit-trees#articles |
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Contenido : |
ABSTRACT.
Acca sellowiana, known as feijoa or pineapple guava, is a diploid, (2n = 2x = 22) outcrossing fruit tree species native to Uruguay and Brazil. The species stands out for
its highly aromatic fruits, with nutraceutical and therapeutic value. Despite its promising agronomical value, genetic studies on this species are limited. Linkage genetic maps are valuable tools for genetic and genomic studies, and constitute essential tools in breeding programs to support the development of molecular breeding strategies. A high-density composite genetic linkage map of A. sellowiana was constructed using two genetically connected populations: H5 (TCO × BR, N = 160) and H6 (TCO × DP, N = 184). Genotyping by sequencing (GBS) approach was successfully applied for developing single nucleotide polymorphism (SNP) markers. A total of 4,921 SNP markers were identified using the reference genome of the closely related species Eucalyptus grandis, whereas other 4,656 SNPs were discovered using a de novo pipeline. The individual H5 and H6 maps comprised 1,236 and 1,302 markers distributed over the expected 11 linkage groups, respectively. These two maps spanned a map length of 1,593 and 1,572 cM, with an average inter-marker distance of 1.29 and 1.21 cM, respectively. A large proportion of markers were common to both maps and showed a high degree of collinearity. The composite map consisted of 1,897 SNPs markers with a total map length of 1,314 cM and an average inter-marker distance of 0.69. A novel approach for the construction of composite maps where the meiosis information of individuals of two connected populations is captured in a single estimator is described. A high-density, accurate composite map based on a consensus ordering of markers provides a valuable contribution for future genetic research and breeding efforts in A. sellowiana. A novel mapping approach based on an estimation of multipopulation recombination fraction described here may be applied in the construction of dense composite genetic maps for any other outcrossing diploid species.
© 2021 Quezada, Amadeu, Vignale, Cabrera, Pritsch and Garcia. MenosABSTRACT.
Acca sellowiana, known as feijoa or pineapple guava, is a diploid, (2n = 2x = 22) outcrossing fruit tree species native to Uruguay and Brazil. The species stands out for
its highly aromatic fruits, with nutraceutical and therapeutic value. Despite its promising agronomical value, genetic studies on this species are limited. Linkage genetic maps are valuable tools for genetic and genomic studies, and constitute essential tools in breeding programs to support the development of molecular breeding strategies. A high-density composite genetic linkage map of A. sellowiana was constructed using two genetically connected populations: H5 (TCO × BR, N = 160) and H6 (TCO × DP, N = 184). Genotyping by sequencing (GBS) approach was successfully applied for developing single nucleotide polymorphism (SNP) markers. A total of 4,921 SNP markers were identified using the reference genome of the closely related species Eucalyptus grandis, whereas other 4,656 SNPs were discovered using a de novo pipeline. The individual H5 and H6 maps comprised 1,236 and 1,302 markers distributed over the expected 11 linkage groups, respectively. These two maps spanned a map length of 1,593 and 1,572 cM, with an average inter-marker distance of 1.29 and 1.21 cM, respectively. A large proportion of markers were common to both maps and showed a high degree of collinearity. The composite map consisted of 1,897 SNPs markers with a total map length of 1,314 cM and an average inter-marker distance of 0.69.... Presentar Todo |
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Palabras claves : |
Composite genetic map; Genotyping by sequencing; Multiparent family; Pineapple guava. |
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Thesagro : |
ACCA SELLOWIANA; FEIJOA; MYRTACEAE. |
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Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
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URL : |
https://www.frontiersin.org/articles/10.3389/fpls.2021.626811/full
https://www.frontiersin.org/articles/10.3389/fpls.2021.626811/pdf
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Marc : |
LEADER 03974naa a2200289 a 4500
001 1061756
005 2021-02-23
008 2021 bl uuuu u00u1 u #d
024 7 $a10.3389/fpls.2021.626811$2DOI
100 1 $aQUEZADA, M.
245 $aConstruction of a high-density genetic map of Acca sellowiana (Berg.) Burret, an outcrossing species, based on two connected mapping populations.$h[electronic resource]
260 $c2021
500 $aArticle history: Received: 06 November 2020; Accepted: 12 January 2021; Published: 23 February 2021. Edited by: Maria Luisa Badenes, Instituto Valenciano de Investigaciones Agrarias, Spain. Reviewed by: Shouvik Das, Indian Agricultural Research Institute (ICAR), India; Jonathan Elias Maldonado, Pontificia Universidad Católica de Chile, Chile. Correspondence: Antonio Augusto Franco Garcia - augusto.garcia@usp.br Specialty section: This article was submitted to Plant Breeding, a section of the journal Frontiers in Plant Science. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). This article is part of the research topic: Breeding Innovations in Underutilized Temperate Fruit Trees: https://www.frontiersin.org/research-topics/14915/breeding-innovations-in-underutilized-temperate-fruit-trees#articles
520 $aABSTRACT. Acca sellowiana, known as feijoa or pineapple guava, is a diploid, (2n = 2x = 22) outcrossing fruit tree species native to Uruguay and Brazil. The species stands out for its highly aromatic fruits, with nutraceutical and therapeutic value. Despite its promising agronomical value, genetic studies on this species are limited. Linkage genetic maps are valuable tools for genetic and genomic studies, and constitute essential tools in breeding programs to support the development of molecular breeding strategies. A high-density composite genetic linkage map of A. sellowiana was constructed using two genetically connected populations: H5 (TCO × BR, N = 160) and H6 (TCO × DP, N = 184). Genotyping by sequencing (GBS) approach was successfully applied for developing single nucleotide polymorphism (SNP) markers. A total of 4,921 SNP markers were identified using the reference genome of the closely related species Eucalyptus grandis, whereas other 4,656 SNPs were discovered using a de novo pipeline. The individual H5 and H6 maps comprised 1,236 and 1,302 markers distributed over the expected 11 linkage groups, respectively. These two maps spanned a map length of 1,593 and 1,572 cM, with an average inter-marker distance of 1.29 and 1.21 cM, respectively. A large proportion of markers were common to both maps and showed a high degree of collinearity. The composite map consisted of 1,897 SNPs markers with a total map length of 1,314 cM and an average inter-marker distance of 0.69. A novel approach for the construction of composite maps where the meiosis information of individuals of two connected populations is captured in a single estimator is described. A high-density, accurate composite map based on a consensus ordering of markers provides a valuable contribution for future genetic research and breeding efforts in A. sellowiana. A novel mapping approach based on an estimation of multipopulation recombination fraction described here may be applied in the construction of dense composite genetic maps for any other outcrossing diploid species. © 2021 Quezada, Amadeu, Vignale, Cabrera, Pritsch and Garcia.
650 $aACCA SELLOWIANA
650 $aFEIJOA
650 $aMYRTACEAE
653 $aComposite genetic map
653 $aGenotyping by sequencing
653 $aMultiparent family
653 $aPineapple guava
700 1 $aAMADEU, R. R.
700 1 $aVIGNALE, B.
700 1 $aCABRERA, D.
700 1 $aPRITSCH, C.
700 1 $aGARCIA, A. A. F.
773 $tFrontiers in Plant Science, 23 February 2021, Volume 12, Article number 626811. OPEN ACCESS. Doi: https://doi.org/10.3389/fpls.2021.626811
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INIA Las Brujas (LB) |
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 | Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha actual : |
31/05/2022 |
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Actualizado : |
02/12/2022 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
COSTA, M.; SARAVIA, A.; UBIOS, D.; LORES, L.; DA COSTA, V.; FESTARI, M.F.; LANDEIRA, M.; RODRÍGUEZ-ZRAQUIA, S.A.; BANCHERO, G.; FREIRE, T. |
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Afiliación : |
MONIQUE COSTA, Laboratorio de Inmunomodulación y Desarrollo de Vacunas, Departamento de Inmunobiología, Facultad de Medicina, Universidad de La República, Montevideo, Uruguay.; ANDERSON SARAVIA DE MELO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; DIEGO UBIOS, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay.; PABLO LORES, Laboratorio de Inmunomodulación y Desarrollo de Vacunas, Departamento de Inmunobiología, Facultad de Medicina, Universidad de La República, Montevideo, Uruguay.; VALERIA DA COSTA, Laboratorio de Inmunomodulación y Desarrollo de Vacunas, Departamento de Inmunobiología, Facultad de Medicina, Universidad de La República, Montevideo, Uruguay.; MARÍA FLORENCIA FESTARI, Laboratorio de Inmunomodulación y Desarrollo de Vacunas, Departamento de Inmunobiología, Facultad de Medicina, Universidad de La República, Montevideo, Uruguay.; MERCEDES LANDEIRA, Laboratorio de Inmunomodulación y Desarrollo de Vacunas, Departamento de Inmunobiología, Facultad de Medicina, Universidad de La República, Montevideo, Uruguay; SANTIAGO A. RODRÍGUEZ-ZRAQUIA, Laboratorio de Inmunomodulación y Desarrollo de Vacunas, Departamento de Inmunobiología, Facultad de Medicina, Universidad de La República, Montevideo, Uruguay.; GEORGGET ELIZABETH BANCHERO HUNZIKER, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; TERESA FREIRE, Laboratorio de Inmunomodulación y Desarrollo de Vacunas, Departamento de Inmunobiología, Facultad de Medicina, Universidad de La República, Montevideo, Uruguay. |
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Título : |
Liver function markers and haematological dynamics during acute and chronic phases of experimental Fasciola hepatica infection in cattle treated with triclabendazole. |
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Fecha de publicación : |
2022 |
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Fuente / Imprenta : |
Experimental Parasitology, July 2022, Volume 238, e108285. doi://doi.org/10.1016/j.exppara.2022.108285 |
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ISSN : |
0014-4894 |
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DOI : |
10.1016/j.exppara.2022.108285 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 13 September 2021; Revised 24 May 2022; Accepted 27 May 2022; Available online 30 May 2022; Published July 2022. -- Corresponding author : Freire, T.; Facultad de Medicina, Departamento de Inmunobiología. Gral. Flores 2125, Montevideo, Uruguay; email:tfreire@fmed.edu.uy -- |
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Contenido : |
Abstract:
Fasciola hepatica, a worldwide-distributed liver fluke, is one of the causative agents of fasciolosis, a zoonotic disease that affects livestock and humans. In livestock, fasciolosis causes huge economic losses worldwide, reducing animal fertility, milk production, weight gain and condemnation of livers. In spite of the availability of drugs, such as triclabendazole (TCZ), for the treatment of fasciolosis, they do not necessarily prevent liver damage or parasite reinfection and can eventually increase parasite resistance. The aim of this research was to relate the hepatic function, haematological parameters, leukocyte counts in circulation and parasite egg shedding during F. hepatica acute and chronic phases of infection in cattle as well as to determine how these parameters change with TCZ-treatment of chronically infected cattle. Our results show that increased levels of serum aspartate aminotransferase (AST) and gamma glutamyltransferase (GGT) were detected in early stages of the experimental infection. Moreover, high circulating eosinophil count and plateletcrit levels were correlated with fluke number in livers from infected cattle. On the other hand, although TCZ-treatment in the chronic phase of infection reduced parasite burden and damage in the liver, it was not able to completely avoid them. In conclusion, our work sheds light into the physiopathological mechanisms induced during fluke infection in cattle, revealing the complexity of the host response to the infection, together with the effects of TCZ-treatment in chronically infected animals. © 2022 MenosAbstract:
Fasciola hepatica, a worldwide-distributed liver fluke, is one of the causative agents of fasciolosis, a zoonotic disease that affects livestock and humans. In livestock, fasciolosis causes huge economic losses worldwide, reducing animal fertility, milk production, weight gain and condemnation of livers. In spite of the availability of drugs, such as triclabendazole (TCZ), for the treatment of fasciolosis, they do not necessarily prevent liver damage or parasite reinfection and can eventually increase parasite resistance. The aim of this research was to relate the hepatic function, haematological parameters, leukocyte counts in circulation and parasite egg shedding during F. hepatica acute and chronic phases of infection in cattle as well as to determine how these parameters change with TCZ-treatment of chronically infected cattle. Our results show that increased levels of serum aspartate aminotransferase (AST) and gamma glutamyltransferase (GGT) were detected in early stages of the experimental infection. Moreover, high circulating eosinophil count and plateletcrit levels were correlated with fluke number in livers from infected cattle. On the other hand, although TCZ-treatment in the chronic phase of infection reduced parasite burden and damage in the liver, it was not able to completely avoid them. In conclusion, our work sheds light into the physiopathological mechanisms induced during fluke infection in cattle, revealing the complexity of the host response to ... Presentar Todo |
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Palabras claves : |
Fasciola hepatica; Fasciolosis; Fluke; LIVER; PLATAFORMA DE SALUD ANIMAL; Triclabendazole. |
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Asunto categoría : |
L73 Enfermedades de los animales |
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Marc : |
LEADER 02950naa a2200337 a 4500
001 1063184
005 2022-12-02
008 2022 bl uuuu u00u1 u #d
022 $a0014-4894
024 7 $a10.1016/j.exppara.2022.108285$2DOI
100 1 $aCOSTA, M.
245 $aLiver function markers and haematological dynamics during acute and chronic phases of experimental Fasciola hepatica infection in cattle treated with triclabendazole.$h[electronic resource]
260 $c2022
500 $aArticle history: Received 13 September 2021; Revised 24 May 2022; Accepted 27 May 2022; Available online 30 May 2022; Published July 2022. -- Corresponding author : Freire, T.; Facultad de Medicina, Departamento de Inmunobiología. Gral. Flores 2125, Montevideo, Uruguay; email:tfreire@fmed.edu.uy --
520 $aAbstract: Fasciola hepatica, a worldwide-distributed liver fluke, is one of the causative agents of fasciolosis, a zoonotic disease that affects livestock and humans. In livestock, fasciolosis causes huge economic losses worldwide, reducing animal fertility, milk production, weight gain and condemnation of livers. In spite of the availability of drugs, such as triclabendazole (TCZ), for the treatment of fasciolosis, they do not necessarily prevent liver damage or parasite reinfection and can eventually increase parasite resistance. The aim of this research was to relate the hepatic function, haematological parameters, leukocyte counts in circulation and parasite egg shedding during F. hepatica acute and chronic phases of infection in cattle as well as to determine how these parameters change with TCZ-treatment of chronically infected cattle. Our results show that increased levels of serum aspartate aminotransferase (AST) and gamma glutamyltransferase (GGT) were detected in early stages of the experimental infection. Moreover, high circulating eosinophil count and plateletcrit levels were correlated with fluke number in livers from infected cattle. On the other hand, although TCZ-treatment in the chronic phase of infection reduced parasite burden and damage in the liver, it was not able to completely avoid them. In conclusion, our work sheds light into the physiopathological mechanisms induced during fluke infection in cattle, revealing the complexity of the host response to the infection, together with the effects of TCZ-treatment in chronically infected animals. © 2022
653 $aFasciola hepatica
653 $aFasciolosis
653 $aFluke
653 $aLIVER
653 $aPLATAFORMA DE SALUD ANIMAL
653 $aTriclabendazole
700 1 $aSARAVIA, A.
700 1 $aUBIOS, D.
700 1 $aLORES, L.
700 1 $aDA COSTA, V.
700 1 $aFESTARI, M.F.
700 1 $aLANDEIRA, M.
700 1 $aRODRÍGUEZ-ZRAQUIA, S.A.
700 1 $aBANCHERO, G.
700 1 $aFREIRE, T.
773 $tExperimental Parasitology, July 2022, Volume 238, e108285. doi://doi.org/10.1016/j.exppara.2022.108285
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