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Registro completo
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Biblioteca (s) : |
INIA Treinta y Tres. |
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Fecha : |
25/02/2016 |
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Actualizado : |
31/08/2018 |
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Autor : |
AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE; INDIAN NATIONAL SCIENCE ACADEMY; IRRI - INTERNATIONAL RICE RESEARCH INSTITUTE |
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Título : |
Climate and food security. [Proceedings]. |
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Fecha de publicación : |
1989 |
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Fuente / Imprenta : |
Manila (Philippines): IRRI, 1989. |
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Páginas : |
602 p. |
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ISBN : |
971-104-210-x |
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Idioma : |
Inglés |
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Notas : |
Papers presented at the International Symposium on Climate Variabiliy and Food Security in Development Countries, 5-9 February 1987, New Delhi, India. |
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Thesagro : |
AGRICULTURA; ALIMENTACION HUMANA; CAMBIO CLIMATICO; CLIMA; RENDIMIENTOS. |
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Asunto categoría : |
P40 Meteorología y climatología |
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Marc : |
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001 1054353
005 2018-08-31
008 1989 bl uuuu u00u1 u #d
020 $a971-104-210-x
100 1 $aAMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE
245 $aClimate and food security. [Proceedings].
260 $aManila (Philippines): IRRI$c1989
300 $a602 p.
500 $aPapers presented at the International Symposium on Climate Variabiliy and Food Security in Development Countries, 5-9 February 1987, New Delhi, India.
650 $aAGRICULTURA
650 $aALIMENTACION HUMANA
650 $aCAMBIO CLIMATICO
650 $aCLIMA
650 $aRENDIMIENTOS
700 1 $aINDIAN NATIONAL SCIENCE ACADEMY
700 1 $aIRRI - INTERNATIONAL RICE RESEARCH INSTITUTE
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Registro original : |
INIA Treinta y Tres (TT) |
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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
11/03/2021 |
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Actualizado : |
11/03/2021 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
NARANCIO, R.; JOHN, U.; MASON, J.; SPANGENBERG, G. |
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Afiliación : |
RAFAEL NARANCIO FERES, Agriculture Victoria Research, AgriBio Centre for AgriBioscience, La Trobe University, Melbourne, Australia; School of Applied Systems Biology, La Trobe University, Melbourne, Australia; ULRIK JOHN, Agriculture Victoria Research, AgriBio Centre for AgriBioscience, La Trobe University, Melbourne, Australia; JOHN MASON, Agriculture Victoria Research, AgriBio Centre for AgriBioscience, La Trobe University, Melbourne, Australia; School of Applied Systems Biology, La Trobe University, Melbourne, Australia; GERMAN SPANGENBERG, Agriculture Victoria Research, AgriBio Centre for AgriBioscience, La Trobe University, Melbourne, Australia; School of Applied Systems Biology, La Trobe University, Melbourne, Australia. |
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Título : |
Selection of optimal reference genes for quantitative RT-PCR transcript abundance analysis in white clover (Trifolium repens L.). |
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Fecha de publicación : |
2018 |
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Fuente / Imprenta : |
Functional Plant Biology, 2018, Volume 45, Issue 7, Pages 737-744. Doi: https://doi.org/10.1071/FP17304 |
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ISSN : |
1445-4408 |
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DOI : |
10.1071/FP17304 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 1 November 2017; Accepted 21 January 2018; Published online 16 February 2018.
Corresponding author: John, U.; Agriculture Victoria Research, AgriBio Centre for AgriBioscience, La Trobe University, Melbourne, VIC, Australia; email:ulrik.john@ecodev.vic.gov.au |
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Contenido : |
ABSTRACT -
Quantitative reverse transcription PCR (qRT-PCR) is a widely used method for transcript abundance analyses in plants. Relative quantification by qRT-PCR requires the use of a stably expressed reference gene. There are many ?housekeeping? genes reported in different plant species that are used as reference genes. However, it is important that the steady-state mRNA levels of these housekeeping genes are confirmed across different conditions and tissues in each species studied. Prior to this study, no comprehensive work had been performed in identifying optimal reference genes in white clover (Trifolium repens L.). To identify suitable reference genes in white clover, we analysed the transcript abundance stability of seven candidate genes in two organs (leaves and stolons) across two treatments (water-limited and well-watered). DCt, NormFinder and ANOVA tests were carried out to evaluate the mRNA level stability of candidate reference genes. According to the DCt results, the genes with the most stable mRNA levels were EF1a and ACT11. When stability among groups was evaluated by NormFinder, UBQ was the most stable across all organs and treatments.
By multiple criteria, EF1a, followed by ACT11 and UBQ, was the most stably-expressed gene across organs and treatments, and each of these are recommended as reference genes for transcript abundance studies in white clover.
© Copyright 2018 Elsevier B.V., All rights reserved. |
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Palabras claves : |
ACt; Gene Selection; Housekeeping genes; MRNA level stability; NormFinder; Peptide Elongation Factor 1. |
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Asunto categoría : |
F01 Cultivo |
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Marc : |
LEADER 02570naa a2200265 a 4500
001 1061825
005 2021-03-11
008 2018 bl uuuu u00u1 u #d
022 $a1445-4408
024 7 $a10.1071/FP17304$2DOI
100 1 $aNARANCIO, R.
245 $aSelection of optimal reference genes for quantitative RT-PCR transcript abundance analysis in white clover (Trifolium repens L.).$h[electronic resource]
260 $c2018
500 $aArticle history: Received 1 November 2017; Accepted 21 January 2018; Published online 16 February 2018. Corresponding author: John, U.; Agriculture Victoria Research, AgriBio Centre for AgriBioscience, La Trobe University, Melbourne, VIC, Australia; email:ulrik.john@ecodev.vic.gov.au
520 $aABSTRACT - Quantitative reverse transcription PCR (qRT-PCR) is a widely used method for transcript abundance analyses in plants. Relative quantification by qRT-PCR requires the use of a stably expressed reference gene. There are many ?housekeeping? genes reported in different plant species that are used as reference genes. However, it is important that the steady-state mRNA levels of these housekeeping genes are confirmed across different conditions and tissues in each species studied. Prior to this study, no comprehensive work had been performed in identifying optimal reference genes in white clover (Trifolium repens L.). To identify suitable reference genes in white clover, we analysed the transcript abundance stability of seven candidate genes in two organs (leaves and stolons) across two treatments (water-limited and well-watered). DCt, NormFinder and ANOVA tests were carried out to evaluate the mRNA level stability of candidate reference genes. According to the DCt results, the genes with the most stable mRNA levels were EF1a and ACT11. When stability among groups was evaluated by NormFinder, UBQ was the most stable across all organs and treatments. By multiple criteria, EF1a, followed by ACT11 and UBQ, was the most stably-expressed gene across organs and treatments, and each of these are recommended as reference genes for transcript abundance studies in white clover. © Copyright 2018 Elsevier B.V., All rights reserved.
653 $aACt
653 $aGene Selection
653 $aHousekeeping genes
653 $aMRNA level stability
653 $aNormFinder
653 $aPeptide Elongation Factor 1
700 1 $aJOHN, U.
700 1 $aMASON, J.
700 1 $aSPANGENBERG, G.
773 $tFunctional Plant Biology, 2018, Volume 45, Issue 7, Pages 737-744. Doi: https://doi.org/10.1071/FP17304
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