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Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
21/02/2014 |
Actualizado : |
29/10/2019 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
GAIERO, P.; MAZZELLA, C.; VILARÓ, F.; SPERANZA, P.; DE JONG, H. |
Afiliación : |
PAOLA GAIERO, Department of Plant Biology, Facultad de Agronomía, Universidad de la República, Uruguay; Laboratory of Genetics, Wageningen University, Netherlands; CRISTINA MAZZELLA, Department of Plant Biology, Facultad de Agronomía, Universidad de la República, Uruguay; FRANCISCO LUIS VILARO PAREJA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; PABLO SPERANZA, Department of Plant Biology, Facultad de Agronomía, Universidad de la República, Uruguay; HANS DE JONG, Laboratory of Genetics, Wageningen University, Netherlands. |
Título : |
Pairing analysis and in situ Hybridisation reveal autopolyploid-like behaviour in Solanum commersonii × S. tuberosum (potato) interspecific hybrids. |
Fecha de publicación : |
2017 |
Fuente / Imprenta : |
Euphytica, 1 July 2017, Volume 213, Issue 7, Article number 137. OPEN ACCESS. |
ISSN : |
0014-2336 |
DOI : |
10.1007/s10681-017-1922-4 |
Idioma : |
Inglés |
Notas : |
Article history: Received: 29 December 2016 / Accepted: 29 May 2017 / Published online: 14 June 2017. |
Contenido : |
ABSTRACT.
Wild potato relatives are rich sources of desirable traits for introgressive hybridisation into cultivated potato. One of them, Solanum commersonii (2n = 2x = 24, 1EBN, endosperm balance number), is an important species belonging to the potato tertiary genepool. It can be used in potato breeding through bridge crosses and 2n gamete production. Triploid F1 hybrids between S. commersonii (through spontaneous 2n egg formation) and diploid 2EBN Solanum tuberosum Group Phureja were crossed with S. tuberosum Group Tuberosum, resulting in successive backcross 1, 2 and 3 progenies. The main aim of this study was to determine if there are any barriers to homoeologous pairing and recombination in the allotriploid (S. commersonii × potato) hybrids and their backcrosses, and so to predict if S. commersonii chromosomes can be transmitted to the next generation and introgressed into their recipient potato chromosomes. Microscopic observations of spread pollen mother cells suggested no preferential pairing in the triploid hybrids, while chromosome transmission and segregation in further meiotic stages were fairly balanced. Fluorescent in situ hybridisation with BAC probes (BAC FISH) was used to obtain markers to trace the meiotic behaviour of specific chromosome pairs. Moreover, genomic in situ hybridisation (GISH) demonstrated no obvious differences in fluorescence signals between the homoeologues suggesting that repetitive sequences did not diverge much between the parental species. As a consequence, we were not able to trace the course of the S. commersonii chromosomes in the successive introgressive hybridisation backcross generations. Our results strongly point at a high genomic similarity between the homoeologous chromosomes promising high suitability of S. commersonii in introgressive hybridisation breeding of potato.
© 2017, The Author(s). MenosABSTRACT.
Wild potato relatives are rich sources of desirable traits for introgressive hybridisation into cultivated potato. One of them, Solanum commersonii (2n = 2x = 24, 1EBN, endosperm balance number), is an important species belonging to the potato tertiary genepool. It can be used in potato breeding through bridge crosses and 2n gamete production. Triploid F1 hybrids between S. commersonii (through spontaneous 2n egg formation) and diploid 2EBN Solanum tuberosum Group Phureja were crossed with S. tuberosum Group Tuberosum, resulting in successive backcross 1, 2 and 3 progenies. The main aim of this study was to determine if there are any barriers to homoeologous pairing and recombination in the allotriploid (S. commersonii × potato) hybrids and their backcrosses, and so to predict if S. commersonii chromosomes can be transmitted to the next generation and introgressed into their recipient potato chromosomes. Microscopic observations of spread pollen mother cells suggested no preferential pairing in the triploid hybrids, while chromosome transmission and segregation in further meiotic stages were fairly balanced. Fluorescent in situ hybridisation with BAC probes (BAC FISH) was used to obtain markers to trace the meiotic behaviour of specific chromosome pairs. Moreover, genomic in situ hybridisation (GISH) demonstrated no obvious differences in fluorescence signals between the homoeologues suggesting that repetitive sequences did not diverge much between the parental spe... Presentar Todo |
Palabras claves : |
BAC FISH; GISH; Introgressive hybridisation; Pairing behaviour; Potato wild relatives. |
Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
URL : |
https://link.springer.com/content/pdf/10.1007%2Fs10681-017-1922-4.pdf
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Marc : |
LEADER 02814naa a2200265 a 4500 001 1012827 005 2019-10-29 008 2017 bl uuuu u00u1 u #d 022 $a0014-2336 024 7 $a10.1007/s10681-017-1922-4$2DOI 100 1 $aGAIERO, P. 245 $aPairing analysis and in situ Hybridisation reveal autopolyploid-like behaviour in Solanum commersonii × S. tuberosum (potato) interspecific hybrids.$h[electronic resource] 260 $c2017 500 $aArticle history: Received: 29 December 2016 / Accepted: 29 May 2017 / Published online: 14 June 2017. 520 $aABSTRACT. Wild potato relatives are rich sources of desirable traits for introgressive hybridisation into cultivated potato. One of them, Solanum commersonii (2n = 2x = 24, 1EBN, endosperm balance number), is an important species belonging to the potato tertiary genepool. It can be used in potato breeding through bridge crosses and 2n gamete production. Triploid F1 hybrids between S. commersonii (through spontaneous 2n egg formation) and diploid 2EBN Solanum tuberosum Group Phureja were crossed with S. tuberosum Group Tuberosum, resulting in successive backcross 1, 2 and 3 progenies. The main aim of this study was to determine if there are any barriers to homoeologous pairing and recombination in the allotriploid (S. commersonii × potato) hybrids and their backcrosses, and so to predict if S. commersonii chromosomes can be transmitted to the next generation and introgressed into their recipient potato chromosomes. Microscopic observations of spread pollen mother cells suggested no preferential pairing in the triploid hybrids, while chromosome transmission and segregation in further meiotic stages were fairly balanced. Fluorescent in situ hybridisation with BAC probes (BAC FISH) was used to obtain markers to trace the meiotic behaviour of specific chromosome pairs. Moreover, genomic in situ hybridisation (GISH) demonstrated no obvious differences in fluorescence signals between the homoeologues suggesting that repetitive sequences did not diverge much between the parental species. As a consequence, we were not able to trace the course of the S. commersonii chromosomes in the successive introgressive hybridisation backcross generations. Our results strongly point at a high genomic similarity between the homoeologous chromosomes promising high suitability of S. commersonii in introgressive hybridisation breeding of potato. © 2017, The Author(s). 653 $aBAC FISH 653 $aGISH 653 $aIntrogressive hybridisation 653 $aPairing behaviour 653 $aPotato wild relatives 700 1 $aMAZZELLA, C. 700 1 $aVILARÓ, F. 700 1 $aSPERANZA, P. 700 1 $aDE JONG, H. 773 $tEuphytica, 1 July 2017, Volume 213, Issue 7, Article number 137. OPEN ACCESS.
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INIA Las Brujas (LB) |
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Biblioteca (s) : |
INIA Tacuarembó. |
Fecha actual : |
20/10/2017 |
Actualizado : |
10/10/2018 |
Tipo de producción científica : |
Abstracts/Resúmenes |
Autor : |
BALMELLI, G.; SIMETO, S.; TORRES, D.; HIRIGOYEN, A.; CASTILLO, A.; ALTIER, N.; PÉREZ, G.; DIEZ, J. |
Afiliación : |
GUSTAVO DANIEL BALMELLI HERNANDEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; SOFIA SIMETO FERRARI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; DIEGO GABRIEL TORRES DINI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; NORA ADRIANA ALTIER MANZINI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
Productivity losses caused by Teratosphaeria nubilosa on Eucalyptus globulus and Eucalyptus maidenii in Uruguay. [Resumen]. |
Fecha de publicación : |
2017 |
Fuente / Imprenta : |
In: Anniversary Congress, 125th, 19-22 September, Freiburg, Germany, 2017. |
Páginas : |
p. 187 |
Idioma : |
Inglés |
Contenido : |
Teratosphaeria nubilosa severely affects young plantations of Eucalyptus globulus and E. maidenii in Uruguay. This work analyzes the
effect of this pathogen on the growth and survival of both species, based on data from a naturally infected field trial. The degree of defoliation was quantified at 21 months old, and its effects on diameter and mortality were evaluated at 62 months old. Defoliation in E. globulus was higher than in E. maidenii, with an average of 52.5 and 28.8%, respectively. Four years after the epidemic, defoliation classes of 40% or higher had significant growth losses on E. globulus, while losses in E. maidenii were significant when defoliation was 30% or higher. An average loss in DAP of 27.5 % in E. globulus and 12.5 % in E. maidenii was observed. The most affected trees, with 70-80% of defoliation in E. globulus and 60% in E. maidenii, had similar growth loss in both species, suffering a reduction in DAP of 43.9% and 42.5%, respectively. By contrast, the average mortality that occurred between 21 and 62 months old was significantly higher in E. globulus (16.7%) than in E. maidenii (3.3%). Mortality in the higher defoliation classes reached 44.7% on E. globulus and 47.1% on E. maidenii. Although E. globulus showed greater susceptibility to the disease and higher productivity losses, the significant defoliation and growth losses registered in E. maidenii suggest that this species is not as suitable as previously thought to replace E. globulus on sites with high risk of T. nubilosa. MenosTeratosphaeria nubilosa severely affects young plantations of Eucalyptus globulus and E. maidenii in Uruguay. This work analyzes the
effect of this pathogen on the growth and survival of both species, based on data from a naturally infected field trial. The degree of defoliation was quantified at 21 months old, and its effects on diameter and mortality were evaluated at 62 months old. Defoliation in E. globulus was higher than in E. maidenii, with an average of 52.5 and 28.8%, respectively. Four years after the epidemic, defoliation classes of 40% or higher had significant growth losses on E. globulus, while losses in E. maidenii were significant when defoliation was 30% or higher. An average loss in DAP of 27.5 % in E. globulus and 12.5 % in E. maidenii was observed. The most affected trees, with 70-80% of defoliation in E. globulus and 60% in E. maidenii, had similar growth loss in both species, suffering a reduction in DAP of 43.9% and 42.5%, respectively. By contrast, the average mortality that occurred between 21 and 62 months old was significantly higher in E. globulus (16.7%) than in E. maidenii (3.3%). Mortality in the higher defoliation classes reached 44.7% on E. globulus and 47.1% on E. maidenii. Although E. globulus showed greater susceptibility to the disease and higher productivity losses, the significant defoliation and growth losses registered in E. maidenii suggest that this species is not as suitable as previously thought to replace E. globulus on sites wit... Presentar Todo |
Palabras claves : |
DEFOLIATION; DISEASE; GROWTH LOSSES; MORTALITY. |
Thesagro : |
ENFERMEDADES DE LAS PLANTAS. |
Asunto categoría : |
H01 Protección de plantas - Aspectos generales |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/7396/1/bALMELLI-2017.pdf
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Marc : |
LEADER 02314nam a2200265 a 4500 001 1057689 005 2018-10-10 008 2017 bl uuuu u00u1 u #d 100 1 $aBALMELLI, G. 245 $aProductivity losses caused by Teratosphaeria nubilosa on Eucalyptus globulus and Eucalyptus maidenii in Uruguay. [Resumen].$h[electronic resource] 260 $aIn: Anniversary Congress, 125th, 19-22 September, Freiburg, Germany$c2017 300 $ap. 187 520 $aTeratosphaeria nubilosa severely affects young plantations of Eucalyptus globulus and E. maidenii in Uruguay. This work analyzes the effect of this pathogen on the growth and survival of both species, based on data from a naturally infected field trial. The degree of defoliation was quantified at 21 months old, and its effects on diameter and mortality were evaluated at 62 months old. Defoliation in E. globulus was higher than in E. maidenii, with an average of 52.5 and 28.8%, respectively. Four years after the epidemic, defoliation classes of 40% or higher had significant growth losses on E. globulus, while losses in E. maidenii were significant when defoliation was 30% or higher. An average loss in DAP of 27.5 % in E. globulus and 12.5 % in E. maidenii was observed. The most affected trees, with 70-80% of defoliation in E. globulus and 60% in E. maidenii, had similar growth loss in both species, suffering a reduction in DAP of 43.9% and 42.5%, respectively. By contrast, the average mortality that occurred between 21 and 62 months old was significantly higher in E. globulus (16.7%) than in E. maidenii (3.3%). Mortality in the higher defoliation classes reached 44.7% on E. globulus and 47.1% on E. maidenii. Although E. globulus showed greater susceptibility to the disease and higher productivity losses, the significant defoliation and growth losses registered in E. maidenii suggest that this species is not as suitable as previously thought to replace E. globulus on sites with high risk of T. nubilosa. 650 $aENFERMEDADES DE LAS PLANTAS 653 $aDEFOLIATION 653 $aDISEASE 653 $aGROWTH LOSSES 653 $aMORTALITY 700 1 $aSIMETO, S. 700 1 $aTORRES, D. 700 1 $aHIRIGOYEN, A. 700 1 $aCASTILLO, A. 700 1 $aALTIER, N. 700 1 $aPÉREZ, G. 700 1 $aDIEZ, J.
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