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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
05/12/2018 |
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Actualizado : |
26/08/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
RUBIO, L.; HERNÁNDEZ, L.; BENÍTEZ, M. J.; ARRUABARRENA, A.; RIVAS, F.; COLINA, R.; MAESO, D. |
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Afiliación : |
LETICIA PAOLA RUBIO CATTANI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; LESTER HERNÁNDEZ RODRÍGUEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay.; MARÍA JOSÉ BENÍTEZ GALEANO, Laboratorio de Virología Molecular, Centro Universitario Regional Litoral Norte (CENUR- Litoral Norte), Universidad de la República, Uruguay; ANA ARRUABARRENA PASCOVICH, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CARLOS FERNANDO RIVAS GRELA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RODNEY COLINA, Laboratorio de Virología Molecular, Centro Universitario Regional Litoral Norte (CENUR- Litoral Norte), Universidad de la República, Uruguay; DIEGO CESAR MAESO TOZZI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Biological and molecular characterization of Uruguayan citrus tristeza virus field isolates. |
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Fecha de publicación : |
2019 |
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Fuente / Imprenta : |
Journal of Plant Pathology, 2019, volume 101, Issue 1, pages 97-105. |
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ISSN : |
1125-4653 // Online ISSN 2239-7264 |
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DOI : |
10.1007/s42161-018-0149-0 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 06 June 2018 // Accepted 29 July 2018 // First Online 13 August 2018 // Published 15 February 2019.
Acknowledgements: This research was funded by Instituto Nacional de Investigación Agropecuaria, Uruguay (project CT-06), through the National Program of Citrus, in collaboration with the Laboratorio de Virología Molecular, Centro Universitario Regional Litoral Norte de la Universidad de la República.
Funding: This study was funded by Instituto Nacional de Investigación Agropecuaria, Uruguay (project CT-06). |
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Contenido : |
ABSTRACT.
Citrus tristeza virus (CTV) is the causal agent of the most important viral disease of citrus. Symptoms that may affect the productive potential of citrus plants are observed in Uruguayan orchards even though resistant rootstocks are used. CTVis fully eliminated in propagative materials by the National Sanitation and Certification Program, but since the virus and its vector are widespread in the country, the risk of infection in the field persists. In this situation, using mild CTV strains in a cross-protection program would be a useful alternative to attempt to increase yield and quality of the local citrus industry. To this aim, this study assessed the biological and molecular characteristics of 32 local CTV isolates. Bioassays were conducted in a greenhouse with controlled conditions. Each isolate was graft-inoculated on Mexican lime, sweet orange, sour orange and Duncan grapefruit indicator plants. Symptoms and their intensity were evaluated. Molecular characterization was carried out by RT-PCR amplification, using primers for the p25, p20 and p23 genes. PCR products were sequenced, nucleotide sequences were aligned with international reference strains and phylogenetic trees were constructed. Results of the biological and molecular analysis showed the prevalence of severe CTV isolates with a high genetic variability. Two out of 32 characterized isolates were selected as mild CTV isolates to be tested as candidates for future cross-protection experiments. The survey showed a complex scenario for the management of CTV in Uruguay.
© Società Italiana di Patologia Vegetale (S.I.Pa.V.) 2018 MenosABSTRACT.
Citrus tristeza virus (CTV) is the causal agent of the most important viral disease of citrus. Symptoms that may affect the productive potential of citrus plants are observed in Uruguayan orchards even though resistant rootstocks are used. CTVis fully eliminated in propagative materials by the National Sanitation and Certification Program, but since the virus and its vector are widespread in the country, the risk of infection in the field persists. In this situation, using mild CTV strains in a cross-protection program would be a useful alternative to attempt to increase yield and quality of the local citrus industry. To this aim, this study assessed the biological and molecular characteristics of 32 local CTV isolates. Bioassays were conducted in a greenhouse with controlled conditions. Each isolate was graft-inoculated on Mexican lime, sweet orange, sour orange and Duncan grapefruit indicator plants. Symptoms and their intensity were evaluated. Molecular characterization was carried out by RT-PCR amplification, using primers for the p25, p20 and p23 genes. PCR products were sequenced, nucleotide sequences were aligned with international reference strains and phylogenetic trees were constructed. Results of the biological and molecular analysis showed the prevalence of severe CTV isolates with a high genetic variability. Two out of 32 characterized isolates were selected as mild CTV isolates to be tested as candidates for future cross-protection experiments. The su... Presentar Todo |
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Palabras claves : |
BIOLOGICAL INDEXING; CITRUS TRISTEZA VIRUS (CTV); CLOSTEROVIRIDAE; CLOSTEROVIRUS; PHYLOGENETIC ANALYSIS. |
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Thesagro : |
CITRUS. |
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Asunto categoría : |
H20 Enfermedades de las plantas |
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Marc : |
LEADER 03065naa a2200301 a 4500
001 1059343
005 2019-08-26
008 2019 bl uuuu u00u1 u #d
022 $a1125-4653 // Online ISSN 2239-7264
024 7 $a10.1007/s42161-018-0149-0$2DOI
100 1 $aRUBIO, L.
245 $aBiological and molecular characterization of Uruguayan citrus tristeza virus field isolates.$h[electronic resource]
260 $c2019
500 $aArticle history: Received 06 June 2018 // Accepted 29 July 2018 // First Online 13 August 2018 // Published 15 February 2019. Acknowledgements: This research was funded by Instituto Nacional de Investigación Agropecuaria, Uruguay (project CT-06), through the National Program of Citrus, in collaboration with the Laboratorio de Virología Molecular, Centro Universitario Regional Litoral Norte de la Universidad de la República. Funding: This study was funded by Instituto Nacional de Investigación Agropecuaria, Uruguay (project CT-06).
520 $aABSTRACT. Citrus tristeza virus (CTV) is the causal agent of the most important viral disease of citrus. Symptoms that may affect the productive potential of citrus plants are observed in Uruguayan orchards even though resistant rootstocks are used. CTVis fully eliminated in propagative materials by the National Sanitation and Certification Program, but since the virus and its vector are widespread in the country, the risk of infection in the field persists. In this situation, using mild CTV strains in a cross-protection program would be a useful alternative to attempt to increase yield and quality of the local citrus industry. To this aim, this study assessed the biological and molecular characteristics of 32 local CTV isolates. Bioassays were conducted in a greenhouse with controlled conditions. Each isolate was graft-inoculated on Mexican lime, sweet orange, sour orange and Duncan grapefruit indicator plants. Symptoms and their intensity were evaluated. Molecular characterization was carried out by RT-PCR amplification, using primers for the p25, p20 and p23 genes. PCR products were sequenced, nucleotide sequences were aligned with international reference strains and phylogenetic trees were constructed. Results of the biological and molecular analysis showed the prevalence of severe CTV isolates with a high genetic variability. Two out of 32 characterized isolates were selected as mild CTV isolates to be tested as candidates for future cross-protection experiments. The survey showed a complex scenario for the management of CTV in Uruguay. © Società Italiana di Patologia Vegetale (S.I.Pa.V.) 2018
650 $aCITRUS
653 $aBIOLOGICAL INDEXING
653 $aCITRUS TRISTEZA VIRUS (CTV)
653 $aCLOSTEROVIRIDAE
653 $aCLOSTEROVIRUS
653 $aPHYLOGENETIC ANALYSIS
700 1 $aHERNÁNDEZ, L.
700 1 $aBENÍTEZ, M. J.
700 1 $aARRUABARRENA, A.
700 1 $aRIVAS, F.
700 1 $aCOLINA, R.
700 1 $aMAESO, D.
773 $tJournal of Plant Pathology, 2019, volume 101, Issue 1, pages 97-105.
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Registro original : |
INIA Las Brujas (LB) |
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Biblioteca
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Identificación
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Origen
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Tipo / Formato
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Clasificación
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Registro
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha actual : |
04/02/2020 |
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Actualizado : |
05/09/2022 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
BRITO, L.B; RIET-CORREA, F.; ALMEIDA, V.M.; SILVA FILHO, G.B.; CHAVES, A.S.; BRAGA, T.C.; NETO, J.E.; MENDONÇA, F.S. |
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Afiliación : |
LUIZ B. BRITO, Post-Graduate Program in Veterinary Medicine, Universidade Federal Rural de Pernambuco, Recife, PE, Brazil; FRANKLIN RIET-CORREA AMARAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; VALDIR M. ALMEIDA, Hospital Veterinário, Centro de Saúde e Tecnologia Rural (CSTR), Universidade Federal de Campina Grande (UFCG), Campus de Patos, Patos, PB, Brazil; GIVALDO B. SILVA FILHO, Laboratório de Diagnóstico Animal, Departamento de Morfologia e Fisiologia Animal, Universidade Federal Rural de Pernambuco (UFRPE), Recife, PE, Brazil; HISADORA A.S. CHAVES5, Laboratório de Diagnóstico Animal, Departamento de Morfologia e Fisiologia Animal, Universidade Federal Rural de Pernambuco (UFRPE), Recife, PE, Brazil; THAIZA C. BRAGA, Laboratório de Diagnóstico Animal, Departamento de Morfologia e Fisiologia Animal, Universidade Federal Rural de Pernambuco (UFRPE), Recife, PE, Brazil; JOAQUIM EVÊNCIO NETO, Laboratório de Diagnóstico Animal, Departamento de Morfologia e Fisiologia Animal, Universidade Federal Rural de Pernambuco (UFRPE), Recife, PE, Brazil; FÁBIO S. MENDONÇA, Laboratório de Diagnóstico Animal, Departamento de Morfologia e Fisiologia Animal, Universidade Federal Rural de Pernambuco (UFRPE), Recife, PE, Brazil. |
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Título : |
Spontaneous poisoning by Ricinus communis leaves (Euphorbiaceae) in goats. [Intoxicação espontânea por folhas de Ricinus communis (Euphorbiaceae) em caprinos.] |
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Fecha de publicación : |
2019 |
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Fuente / Imprenta : |
Pesquisa Veterinaria Brasileira, 1 February 2019, Volume 39, Issue 2, Pages 123-128. OPEN ACCESS. Doi: http://dx.doi.org/10.1590/1678-5150-pvb-5992 |
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ISSN : |
0100-736X (Print) / 1678-5150 (Online) |
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DOI : |
10.1590/1678-5150-pvb-5992 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: August 05, 2018 / Accepted: October 25, 2018.
Corresponding autor: Fábio S. Mendonça - fabio.mendonca@pq.cnpq.br |
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Contenido : |
ABSTRACT.
The aim of this study was to report the clinical and pathological aspects of an outbreak of poisoning by the ingestion of Ricinus communis leaves in a herd of goats at Pernambuco, northeastern Brazil. Within 3-5 hours after ingesting the sprouts and young shrubs of the plant, twenty Toggenburg female goats and two adults crossbred wethers presented acute neurological clinical signs, which were initially characterized by decreased locomotor activity that later evolved to severe ataxia, depression, incoordination and staggering gait. Four goat that died spontaneously were necropsied. Gross lesions were unspecific and consisted in focal areas of lungs edema, petechial hemorrhages in the epicardium and congestion and enlargement of liver. The contents of the rumen, reticulum and omasum were dry and contained leaves of the plant. Histologically there were no lesions in the CNS. In the liver, the main lesion consisted in cytoplasmic vacuolization and necrosis of hepatocytes. Eighteen goats recovered after a supportive therapy with activated charcoal, glycated isotonic solution, dexamethasone and vitamin B12. There is no specific therapy for poisoning by R. communis, however supportive and symptomatic treatments are recommended and should be based on the clinical signs. © 2019 Colegio Brasileiro de Patologia Animal.All rights reserved.
RESUMO.
O objetivo deste estudo foi relatar os aspectos clínicos e patológicos de um surto de intoxicação pelas folhas de Ricinus communis em um rebanho de caprinos em Pernambuco, Nordeste do Brasil. Três a cinco horas após a ingestão dos brotos e arbustos jovens da planta, vinte cabras da raça Toggenburg e dois machos mestiços apresentaram quadro clínico neurológico agudo caracterizado principalmente pela diminuição da atividade locomotora, grave ataxia, depressão, incoordenação e marcha cambaleante. Quatro caprinos morreram espontaneamente e foram necropsiados. Macroscopicamente, as lesões eram inespecíficas e consistiam em áreas focais de edema pulmonar, hemorragias petequiais epicárdicas e aumento do volume e congestão do fígado. Os conteúdos do rumem, retículo e omaso eram ressecados e continham folhas da planta. Histologicamente, não foram observadas lesões no SNC. No fígado, havia vacuolização citoplasmática e necrose de hepatócitos. Dezoito caprinos se recuperaram após receberem terapia de suporte com carvão ativado, soro glicosado, dexametasona e vitamina B12. Não existe terapêutica especifica para a intoxicação pelas folhas de R. Communis. Os tratamentos sintomáticos e de suporte são recomendados e devem basear-se nos sinais clínicos. MenosABSTRACT.
The aim of this study was to report the clinical and pathological aspects of an outbreak of poisoning by the ingestion of Ricinus communis leaves in a herd of goats at Pernambuco, northeastern Brazil. Within 3-5 hours after ingesting the sprouts and young shrubs of the plant, twenty Toggenburg female goats and two adults crossbred wethers presented acute neurological clinical signs, which were initially characterized by decreased locomotor activity that later evolved to severe ataxia, depression, incoordination and staggering gait. Four goat that died spontaneously were necropsied. Gross lesions were unspecific and consisted in focal areas of lungs edema, petechial hemorrhages in the epicardium and congestion and enlargement of liver. The contents of the rumen, reticulum and omasum were dry and contained leaves of the plant. Histologically there were no lesions in the CNS. In the liver, the main lesion consisted in cytoplasmic vacuolization and necrosis of hepatocytes. Eighteen goats recovered after a supportive therapy with activated charcoal, glycated isotonic solution, dexamethasone and vitamin B12. There is no specific therapy for poisoning by R. communis, however supportive and symptomatic treatments are recommended and should be based on the clinical signs. © 2019 Colegio Brasileiro de Patologia Animal.All rights reserved.
RESUMO.
O objetivo deste estudo foi relatar os aspectos clínicos e patológicos de um surto de intoxicação pelas folhas de Ricinus communi... Presentar Todo |
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Palabras claves : |
Ataxia; Depression; Euphorbiaceae; Plant poisoning; Ricinus communis leaves; Ruminants; Toxicoses. |
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Asunto categoría : |
-- |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/16709/1/Pesq.-Vet.-Bras.-392p.123-128-fevereiro-2019.pdf
http://www.scielo.br/pdf/pvb/v39n2/1678-5150-pvb-39-02-123.pdf
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Marc : |
LEADER 03897naa a2200325 a 4500
001 1060760
005 2022-09-05
008 2019 bl uuuu u00u1 u #d
022 $a0100-736X (Print) / 1678-5150 (Online)
024 7 $a10.1590/1678-5150-pvb-5992$2DOI
100 1 $aBRITO, L.B
245 $aSpontaneous poisoning by Ricinus communis leaves (Euphorbiaceae) in goats. [Intoxicação espontânea por folhas de Ricinus communis (Euphorbiaceae) em caprinos.]$h[electronic resource]
260 $c2019
500 $aArticle history: Received: August 05, 2018 / Accepted: October 25, 2018. Corresponding autor: Fábio S. Mendonça - fabio.mendonca@pq.cnpq.br
520 $aABSTRACT. The aim of this study was to report the clinical and pathological aspects of an outbreak of poisoning by the ingestion of Ricinus communis leaves in a herd of goats at Pernambuco, northeastern Brazil. Within 3-5 hours after ingesting the sprouts and young shrubs of the plant, twenty Toggenburg female goats and two adults crossbred wethers presented acute neurological clinical signs, which were initially characterized by decreased locomotor activity that later evolved to severe ataxia, depression, incoordination and staggering gait. Four goat that died spontaneously were necropsied. Gross lesions were unspecific and consisted in focal areas of lungs edema, petechial hemorrhages in the epicardium and congestion and enlargement of liver. The contents of the rumen, reticulum and omasum were dry and contained leaves of the plant. Histologically there were no lesions in the CNS. In the liver, the main lesion consisted in cytoplasmic vacuolization and necrosis of hepatocytes. Eighteen goats recovered after a supportive therapy with activated charcoal, glycated isotonic solution, dexamethasone and vitamin B12. There is no specific therapy for poisoning by R. communis, however supportive and symptomatic treatments are recommended and should be based on the clinical signs. © 2019 Colegio Brasileiro de Patologia Animal.All rights reserved. RESUMO. O objetivo deste estudo foi relatar os aspectos clínicos e patológicos de um surto de intoxicação pelas folhas de Ricinus communis em um rebanho de caprinos em Pernambuco, Nordeste do Brasil. Três a cinco horas após a ingestão dos brotos e arbustos jovens da planta, vinte cabras da raça Toggenburg e dois machos mestiços apresentaram quadro clínico neurológico agudo caracterizado principalmente pela diminuição da atividade locomotora, grave ataxia, depressão, incoordenação e marcha cambaleante. Quatro caprinos morreram espontaneamente e foram necropsiados. Macroscopicamente, as lesões eram inespecíficas e consistiam em áreas focais de edema pulmonar, hemorragias petequiais epicárdicas e aumento do volume e congestão do fígado. Os conteúdos do rumem, retículo e omaso eram ressecados e continham folhas da planta. Histologicamente, não foram observadas lesões no SNC. No fígado, havia vacuolização citoplasmática e necrose de hepatócitos. Dezoito caprinos se recuperaram após receberem terapia de suporte com carvão ativado, soro glicosado, dexametasona e vitamina B12. Não existe terapêutica especifica para a intoxicação pelas folhas de R. Communis. Os tratamentos sintomáticos e de suporte são recomendados e devem basear-se nos sinais clínicos.
653 $aAtaxia
653 $aDepression
653 $aEuphorbiaceae
653 $aPlant poisoning
653 $aRicinus communis leaves
653 $aRuminants
653 $aToxicoses
700 1 $aRIET-CORREA, F.
700 1 $aALMEIDA, V.M.
700 1 $aSILVA FILHO, G.B.
700 1 $aCHAVES, A.S.
700 1 $aBRAGA, T.C.
700 1 $aNETO, J.E.
700 1 $aMENDONÇA, F.S.
773 $tPesquisa Veterinaria Brasileira, 1 February 2019, Volume 39, Issue 2, Pages 123-128. OPEN ACCESS. Doi: http://dx.doi.org/10.1590/1678-5150-pvb-5992
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