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 | Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha : |
18/09/2014 |
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Actualizado : |
26/11/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
SOTELO, M.; IRISARRI, P.; LORITE, M.J.; CASARETTO, E.; REBUFFO, M.; SANJUAN, J.; MONZA, J. |
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Afiliación : |
MONICA IRENE REBUFFO GFELLER, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Diversity of rhizobia nodulating Lotus corniculatus grown in northern and southern regions of Uruguay. |
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Fecha de publicación : |
2012 |
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Fuente / Imprenta : |
Applied Soil Ecology,Volume 49, September 2011, Pages 197-207. |
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ISSN : |
0929-1393 |
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DOI : |
10.1016/j.apsoil.2011.05.005 |
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Idioma : |
Inglés |
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Notas : |
Article history: Article history: Received 6 October 2010/Received in revised form 12 May 2011/Accepted 13 May 2011. |
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Contenido : |
Abstract:
Diversity of rhizobia nodulating Lotus corniculatus grown in geographical regions with different rainfall regimes in northern and southern Uruguay, was estimated using 168 root nodule isolates. ERIC-PCR analysis revealed no correlation between observed fingerprints and the geographical origin of isolates. Despite the commercial strain U510 has been used for decades to inoculate L. corniculatus, none of the isolates corresponded to this strain. Phylogenetic analyses using 16S rRNA and atpD genes, and ITS sequences clustered all the isolates within genus Mesorhizobium. A great majority of the isolates likely belong to the species M. huakuii, as does the commercial strain U510. The remaining isolates were closely related to either M. septentrionale or M. caraganae. Although no M. loti-like bacteria were identified, all isolates carried symbiotic genes closely related to M. loti and other narrow host range Lotus rhizobia. A significant portion of the Uruguayan isolates were as efficient as the reference strain U510 in symbiosis with L. corniculatus. A few of the isolates were also capable of nitrogen fixation in symbiosis with L. uliginosus, albeit with lower efficiency than reference strains. Our results indicate that rhizobia nodulating L. corniculatus in Uruguay are genetically and phenotypically diverse, and that the commercial strain U510 is probably not adapted to survive the Uruguayan edaphoclimatic conditions. |
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Palabras claves : |
16S RRNA; ATPD; ITS; MESORHIZOBIUM; NIFH; NODC. |
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Thesagro : |
LEGUMINOSAS FORRAJERAS; LOTUS CORNICULATUS; LOTUS ULIGINOSUS; RIZOBIOS. |
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Asunto categoría : |
F01 Cultivo |
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Marc : |
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001 1050393
005 2019-11-26
008 2012 bl uuuu u00u1 u #d
022 $a0929-1393
024 7 $a10.1016/j.apsoil.2011.05.005$2DOI
100 1 $aSOTELO, M.
245 $aDiversity of rhizobia nodulating Lotus corniculatus grown in northern and southern regions of Uruguay.$h[electronic resource]
260 $c2012
500 $aArticle history: Article history: Received 6 October 2010/Received in revised form 12 May 2011/Accepted 13 May 2011.
520 $aAbstract: Diversity of rhizobia nodulating Lotus corniculatus grown in geographical regions with different rainfall regimes in northern and southern Uruguay, was estimated using 168 root nodule isolates. ERIC-PCR analysis revealed no correlation between observed fingerprints and the geographical origin of isolates. Despite the commercial strain U510 has been used for decades to inoculate L. corniculatus, none of the isolates corresponded to this strain. Phylogenetic analyses using 16S rRNA and atpD genes, and ITS sequences clustered all the isolates within genus Mesorhizobium. A great majority of the isolates likely belong to the species M. huakuii, as does the commercial strain U510. The remaining isolates were closely related to either M. septentrionale or M. caraganae. Although no M. loti-like bacteria were identified, all isolates carried symbiotic genes closely related to M. loti and other narrow host range Lotus rhizobia. A significant portion of the Uruguayan isolates were as efficient as the reference strain U510 in symbiosis with L. corniculatus. A few of the isolates were also capable of nitrogen fixation in symbiosis with L. uliginosus, albeit with lower efficiency than reference strains. Our results indicate that rhizobia nodulating L. corniculatus in Uruguay are genetically and phenotypically diverse, and that the commercial strain U510 is probably not adapted to survive the Uruguayan edaphoclimatic conditions.
650 $aLEGUMINOSAS FORRAJERAS
650 $aLOTUS CORNICULATUS
650 $aLOTUS ULIGINOSUS
650 $aRIZOBIOS
653 $a16S RRNA
653 $aATPD
653 $aITS
653 $aMESORHIZOBIUM
653 $aNIFH
653 $aNODC
700 1 $aIRISARRI, P.
700 1 $aLORITE, M.J.
700 1 $aCASARETTO, E.
700 1 $aREBUFFO, M.
700 1 $aSANJUAN, J.
700 1 $aMONZA, J.
773 $tApplied Soil Ecology,Volume 49, September 2011, Pages 197-207.
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