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| Registros recuperados : 238 | |
| 32. |  | ROVIRA, P.J. Inocuidad de carnes: pasado, presente, futuro. [Resumen] Biotecnología Animal In: REDBIO; INIA (Instituto Nacional de Investigación Agropecuaria); REDBIO Argentina. X Encuentro Latinoamericano y del Caribe de Biotecnología Agropecuaria y XI Simposio Redbio Argentina. Libro de Resúmenes. Montevideo 12 - 15 Noviembre 2019. Montevideo (UY): INIA, 2019. p. 158. (INIA Serie Técnica; 253)| Biblioteca(s): INIA Las Brujas. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
27/02/2023 |
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Actualizado : |
15/03/2023 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
ROVIRA, P.J. |
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Afiliación : |
PABLO JUAN ROVIRA SANZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Short-term impact of oxytetracycline administration on the fecal microbiome, resistome and virulome of grazing cattle. |
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Fecha de publicación : |
2023 |
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Fuente / Imprenta : |
Antibiotics, 2023, 12(3):470. OPEN ACCESS. doi: https://doi.org/10.3390/antibiotics12030470 |
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ISSN : |
eISSN 2079-6382 |
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DOI : |
10.3390/antibiotics12030470 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 27 December 2022; Revised 14 February 2023; Accepted 22 February 2023; Published 25 February 2023. -- Academic Editor: Carlos M. Franco. -- Funding: This research was funded by the Instituto Nacional de Investigación Agropecuaria (INIA Uruguay), grant number INIA CL 48. The APC was funded by INIA CL 48 and INIA CL 52. -- This article belongs to the Special Issue Antimicrobial Stewardship in Livestock (https://www.mdpi.com/journal/antibiotics/special_issues/Livestock_Antibiotics). -- LICENSE: Licensee MDPI, Basel, Switzerland. This article is an open access article
distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
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Contenido : |
ABSTRACT.- Antimicrobial resistance (AMR) is an important public health concern around the world. Limited information exists about AMR in grasslands-based systems where antibiotics are seldom used in beef cattle. The present study investigated the impacts of oxytetracycline (OTC) on the microbiome, antibiotic resistance genes (ARGs), and virulence factor genes (VFGs) in grazing steers with no previous exposure to antibiotic treatments. Four steers were injected with a single dose of OTC (TREAT), and four steers were kept as control (CONT). The effects of OTC on fecal microbiome, ARGs, and VFGs were assessed for 14 days using 16S rRNA sequencing and shotgun metagenomics. Alpha and beta microbiome diversities were significantly affected by OTC. Following treatment, less than 8% of bacterial genera had differential abundance between CONT and TREAT samples. Seven ARGs conferring resistance to tetracycline (tet32, tet40, tet44, tetO, tetQ, tetW, and tetW/N/W) increased their abundance in the post-TREAT samples compared to CONT samples. In addition, OTC use was associated with the enrichment of macrolide and lincosamide ARGs (mel and lnuC, respectively). The use of OTC had no significant effect on VFGs. In conclusion, OTC induced short-term alterations of the fecal microbiome and enrichment of ARGs in the feces of grazing beef cattle. Copyright: © 2023 by the author. |
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Palabras claves : |
Antibiotic resistance genes; Fecal microbiome; Grazing cattle; Oxytetracycline; Virulence. |
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Asunto categoría : |
L01 Ganadería |
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URL : |
https://www.mdpi.com/2079-6382/12/3/470/pdf
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Marc : |
LEADER 02843naa a2200217 a 4500
001 1063962
005 2023-03-15
008 2023 bl uuuu u00u1 u #d
022 $aeISSN 2079-6382
024 7 $a10.3390/antibiotics12030470$2DOI
100 1 $aROVIRA, P.J.
245 $aShort-term impact of oxytetracycline administration on the fecal microbiome, resistome and virulome of grazing cattle.$h[electronic resource]
260 $c2023
500 $aArticle history: Received 27 December 2022; Revised 14 February 2023; Accepted 22 February 2023; Published 25 February 2023. -- Academic Editor: Carlos M. Franco. -- Funding: This research was funded by the Instituto Nacional de Investigación Agropecuaria (INIA Uruguay), grant number INIA CL 48. The APC was funded by INIA CL 48 and INIA CL 52. -- This article belongs to the Special Issue Antimicrobial Stewardship in Livestock (https://www.mdpi.com/journal/antibiotics/special_issues/Livestock_Antibiotics). -- LICENSE: Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
520 $aABSTRACT.- Antimicrobial resistance (AMR) is an important public health concern around the world. Limited information exists about AMR in grasslands-based systems where antibiotics are seldom used in beef cattle. The present study investigated the impacts of oxytetracycline (OTC) on the microbiome, antibiotic resistance genes (ARGs), and virulence factor genes (VFGs) in grazing steers with no previous exposure to antibiotic treatments. Four steers were injected with a single dose of OTC (TREAT), and four steers were kept as control (CONT). The effects of OTC on fecal microbiome, ARGs, and VFGs were assessed for 14 days using 16S rRNA sequencing and shotgun metagenomics. Alpha and beta microbiome diversities were significantly affected by OTC. Following treatment, less than 8% of bacterial genera had differential abundance between CONT and TREAT samples. Seven ARGs conferring resistance to tetracycline (tet32, tet40, tet44, tetO, tetQ, tetW, and tetW/N/W) increased their abundance in the post-TREAT samples compared to CONT samples. In addition, OTC use was associated with the enrichment of macrolide and lincosamide ARGs (mel and lnuC, respectively). The use of OTC had no significant effect on VFGs. In conclusion, OTC induced short-term alterations of the fecal microbiome and enrichment of ARGs in the feces of grazing beef cattle. Copyright: © 2023 by the author.
653 $aAntibiotic resistance genes
653 $aFecal microbiome
653 $aGrazing cattle
653 $aOxytetracycline
653 $aVirulence
773 $tAntibiotics, 2023, 12(3):470. OPEN ACCESS. doi: https://doi.org/10.3390/antibiotics12030470
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