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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
18/10/2017 |
Actualizado : |
15/10/2019 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
BOSCHI, F.; SCHVARTZMAN, C.; MURCHIO, S.; FERREIRA, V.; SIRI, M.; GALVÁN, G.; SMOKER, M.; STRANSFEL, L.; ZYPFEL, C.; VILARÓ, F.; DALLA RIZZA, M. |
Afiliación : |
FEDERICO BOSCHI, INASE (Instituto Nacional de Semillas).; CLAUDIA SCHVARTZMAN DISEGNI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARIA SARA MURCHIO VIGNOLO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; VIRGINIA FERREIRA, Universidad de la República (UdelaR)/ Facultad de Química; MARÍA SIRI, Universidad de la República (UdelaR)/ Facultad de Química; GUILLERMO GALVÁN, Universidad de la República (UdelaR)/ Facultad de Agronomía; MATTHEW SMOKER, The Sainsbury Laboratory, Norwich Research Park, Norwich, United Kingdom; LENA STRANSFEL, The Sainsbury Laboratory, Norwich Research Park, Norwich, United Kingdom; CYRIL ZYPFEL, The Sainsbury Laboratory, Norwich Research Park, Norwich, United Kingdom; FRANCISCO LUIS VILARO PAREJA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARCO DALLA RIZZA VILARO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
Enhanced bacterial wilt resistance in potato through expression of arabidopsis efr and introgression of quantitative resistance from solanum commersonii. |
Fecha de publicación : |
2017 |
Fuente / Imprenta : |
Frontiers in Plant Sciences, Volume 8, 25 September 2017, Article number 1642. OPEN ACCESS. |
DOI : |
10.3389/fpls.2017.01642 |
Idioma : |
Inglés |
Notas : |
THIS ARTICLE IS PART OF THE RESEARCH TOPIC: Plant Pathogenic Ralstonia spp. From the Field to the Lab and Back Again: mechanisms of pathogen virulence and host resistance, population biology, community ecology and strategies for bacterial wilt disease management.
Article history: Received: 29 May 2017 / Accepted: 07 September 2017 / Published: 25 September 2017. |
Contenido : |
ABSTRACT.
Bacterial wilt (BW) caused by Ralstonia solanacearum is responsible for substantial losses in cultivated potato (Solanum tuberosum) crops worldwide. Resistance genes have been identified in wild species; however, introduction of these through classical breeding has achieved only partial resistance, which has been linked to poor agronomic performance. The Arabidopsis thaliana (At) pattern recognition receptor elongation factor-Tu (EF-Tu) receptor (EFR) recognizes the bacterial pathogen-associated molecular pattern EF-Tu (and its derived peptide elf18) to confer anti-bacterial immunity. Previous work has shown that transfer of AtEFR into tomato confers increased resistance to R. solanacearum. Here, we evaluated whether the transgenic expression of AtEFR would similarly increase BW resistance in a commercial potato line (INIA Iporá), as well as in a breeding potato line (09509.6) in which quantitative resistance has been introgressed from the wild potato relative Solanum commersonii. Resistance to R. solanacearum was evaluated by damaged root inoculation under controlled conditions. Both INIA Iporá and 09509.6 potato lines expressing AtEFR showed greater resistance to R. solanacearum, with no detectable bacteria in tubers evaluated by multiplex-PCR and plate counting. Notably, AtEFR expression and the introgression of quantitative resistance from S. commersonii had a significant additive effect in 09509.6-AtEFR lines. These results show that the combination of heterologous expression of AtEFR with quantitative resistance introgressed from wild relatives is a promising strategy to develop BW resistance in potato.
© 2017 Boschi, Schvartzman, Murchio, Ferreira, Siri, Galván, Smoker, Stransfeld, Zipfel, Vilaró and Dalla-Rizza MenosABSTRACT.
Bacterial wilt (BW) caused by Ralstonia solanacearum is responsible for substantial losses in cultivated potato (Solanum tuberosum) crops worldwide. Resistance genes have been identified in wild species; however, introduction of these through classical breeding has achieved only partial resistance, which has been linked to poor agronomic performance. The Arabidopsis thaliana (At) pattern recognition receptor elongation factor-Tu (EF-Tu) receptor (EFR) recognizes the bacterial pathogen-associated molecular pattern EF-Tu (and its derived peptide elf18) to confer anti-bacterial immunity. Previous work has shown that transfer of AtEFR into tomato confers increased resistance to R. solanacearum. Here, we evaluated whether the transgenic expression of AtEFR would similarly increase BW resistance in a commercial potato line (INIA Iporá), as well as in a breeding potato line (09509.6) in which quantitative resistance has been introgressed from the wild potato relative Solanum commersonii. Resistance to R. solanacearum was evaluated by damaged root inoculation under controlled conditions. Both INIA Iporá and 09509.6 potato lines expressing AtEFR showed greater resistance to R. solanacearum, with no detectable bacteria in tubers evaluated by multiplex-PCR and plate counting. Notably, AtEFR expression and the introgression of quantitative resistance from S. commersonii had a significant additive effect in 09509.6-AtEFR lines. These results show that the combination of heterol... Presentar Todo |
Palabras claves : |
BACTERIAL WILT; EFR; PATTERN RECOGNITION RECEPTOR; POTATO; QUANTITATIVE RESISTANCE; RALSTONIA SOLANACEARUM. |
Thesagro : |
PAPA; SOLANUM COMMERSONII; SOLANUM TUBEROSUM. |
Asunto categoría : |
-- |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/7375/1/Frontiers-in-Plants-2017-fpls-08-01642.pdf
https://www.frontiersin.org/articles/10.3389/fpls.2017.01642/full
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Marc : |
LEADER 03254naa a2200373 a 4500 001 1057663 005 2019-10-15 008 2017 bl uuuu u00u1 u #d 024 7 $a10.3389/fpls.2017.01642$2DOI 100 1 $aBOSCHI, F. 245 $aEnhanced bacterial wilt resistance in potato through expression of arabidopsis efr and introgression of quantitative resistance from solanum commersonii.$h[electronic resource] 260 $c2017 500 $aTHIS ARTICLE IS PART OF THE RESEARCH TOPIC: Plant Pathogenic Ralstonia spp. From the Field to the Lab and Back Again: mechanisms of pathogen virulence and host resistance, population biology, community ecology and strategies for bacterial wilt disease management. Article history: Received: 29 May 2017 / Accepted: 07 September 2017 / Published: 25 September 2017. 520 $aABSTRACT. Bacterial wilt (BW) caused by Ralstonia solanacearum is responsible for substantial losses in cultivated potato (Solanum tuberosum) crops worldwide. Resistance genes have been identified in wild species; however, introduction of these through classical breeding has achieved only partial resistance, which has been linked to poor agronomic performance. The Arabidopsis thaliana (At) pattern recognition receptor elongation factor-Tu (EF-Tu) receptor (EFR) recognizes the bacterial pathogen-associated molecular pattern EF-Tu (and its derived peptide elf18) to confer anti-bacterial immunity. Previous work has shown that transfer of AtEFR into tomato confers increased resistance to R. solanacearum. Here, we evaluated whether the transgenic expression of AtEFR would similarly increase BW resistance in a commercial potato line (INIA Iporá), as well as in a breeding potato line (09509.6) in which quantitative resistance has been introgressed from the wild potato relative Solanum commersonii. Resistance to R. solanacearum was evaluated by damaged root inoculation under controlled conditions. Both INIA Iporá and 09509.6 potato lines expressing AtEFR showed greater resistance to R. solanacearum, with no detectable bacteria in tubers evaluated by multiplex-PCR and plate counting. Notably, AtEFR expression and the introgression of quantitative resistance from S. commersonii had a significant additive effect in 09509.6-AtEFR lines. These results show that the combination of heterologous expression of AtEFR with quantitative resistance introgressed from wild relatives is a promising strategy to develop BW resistance in potato. © 2017 Boschi, Schvartzman, Murchio, Ferreira, Siri, Galván, Smoker, Stransfeld, Zipfel, Vilaró and Dalla-Rizza 650 $aPAPA 650 $aSOLANUM COMMERSONII 650 $aSOLANUM TUBEROSUM 653 $aBACTERIAL WILT 653 $aEFR 653 $aPATTERN RECOGNITION RECEPTOR 653 $aPOTATO 653 $aQUANTITATIVE RESISTANCE 653 $aRALSTONIA SOLANACEARUM 700 1 $aSCHVARTZMAN, C. 700 1 $aMURCHIO, S. 700 1 $aFERREIRA, V. 700 1 $aSIRI, M. 700 1 $aGALVÁN, G. 700 1 $aSMOKER, M. 700 1 $aSTRANSFEL, L. 700 1 $aZYPFEL, C. 700 1 $aVILARÓ, F. 700 1 $aDALLA RIZZA, M. 773 $tFrontiers in Plant Sciences, Volume 8, 25 September 2017, Article number 1642. OPEN ACCESS.
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INIA Las Brujas (LB) |
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha actual : |
03/07/2023 |
Actualizado : |
03/07/2023 |
Tipo de producción científica : |
Artículos en Revistas Agropecuarias |
Autor : |
AMOROS, M.; GALVAN, V.; RODRIGUEZ, A.; AMARAL, J.; ROSSINI, C.; BUENAHORA, J. |
Afiliación : |
MARIA EUGENIA AMOROS MARTINEZ, Laboratorio de Ecología Química, Facultad de Química - Udelar; VERONICA FRANCISCA GALVAN DA CRUZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ABEL EMMANUEL RODRIGUEZ PEDETTI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JUAN ANTONIO AMARAL SORIA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CARMEN ROSSINI, Laboratorio de Ecología Química, Facultad de Química - Udelar; JOSE HERMES BUENAHORA ACOSTA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
Nuevas alternativas de bajo impacto para el control de ninfas de Diaphorina citry y su efecto sobre el parasitoide Tamarixia radiata. |
Complemento del título : |
Hortifruticultura. |
Fecha de publicación : |
2023 |
Fuente / Imprenta : |
Revista INIA Uruguay, Junio 2023, no.73, p.47-51. |
Serie : |
(Revista INIA; 73). |
ISSN : |
1510-9011 |
Idioma : |
Español |
Contenido : |
El HLB es la enfermedad más destructiva de los cítricos en el mundo, y ha sido recientemente detectada en nuestro país, en la localidad de Bella Unión. El control de su vector, Diaphorina citri, es un pilar fundamental en el manejo de esta enfermedad. En el contexto de una citricultura de exportación de fruta para consumo en fresco, este control debe enmarcarse en un manejo integrado de plagas. El monitoreo y el uso de productos de bajo impacto, que sean compatibles con el control biológico y no generen residuos en fruta, son aspectos fundamentales a considerar. |
Palabras claves : |
HUANGLONGBING (HLB); SISTEMA VEGETAL INTENSIVO - INIA. |
Thesagro : |
CITRUS; DIAPHORINA CITRI. |
Asunto categoría : |
A50 Investigación agraria |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/17262/1/Revista-INIA-73-junio-2023-13.pdf
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Marc : |
LEADER 01345naa a2200253 a 4500 001 1064229 005 2023-07-03 008 2023 bl uuuu u00u1 u #d 022 $a1510-9011 100 1 $aAMOROS, M. 245 $aNuevas alternativas de bajo impacto para el control de ninfas de Diaphorina citry y su efecto sobre el parasitoide Tamarixia radiata.$h[electronic resource] 260 $c2023 490 $a(Revista INIA; 73). 520 $aEl HLB es la enfermedad más destructiva de los cítricos en el mundo, y ha sido recientemente detectada en nuestro país, en la localidad de Bella Unión. El control de su vector, Diaphorina citri, es un pilar fundamental en el manejo de esta enfermedad. En el contexto de una citricultura de exportación de fruta para consumo en fresco, este control debe enmarcarse en un manejo integrado de plagas. El monitoreo y el uso de productos de bajo impacto, que sean compatibles con el control biológico y no generen residuos en fruta, son aspectos fundamentales a considerar. 650 $aCITRUS 650 $aDIAPHORINA CITRI 653 $aHUANGLONGBING (HLB) 653 $aSISTEMA VEGETAL INTENSIVO - INIA 700 1 $aGALVAN, V. 700 1 $aRODRIGUEZ, A. 700 1 $aAMARAL, J. 700 1 $aROSSINI, C. 700 1 $aBUENAHORA, J. 773 $tRevista INIA Uruguay, Junio 2023, no.73, p.47-51.
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