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Biblioteca (s) : |
INIA La Estanzuela. |
Fecha : |
07/10/2014 |
Actualizado : |
05/11/2019 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
ALARCÓN-REVERTE, R.; GARCIA, M.A.; WATSON, S.B.; ABDALLAH, I.; SABATÉ, S.; HERNÁNDEZ, M.J.; DAYAN ,F.E.; FISCHER, A.J. |
Afiliación : |
MILTON ALEJANDRO GARCIA LATASA, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. |
Título : |
Concerted action of target-site mutations and high EPSPS activity in glyphosate-resistant junglerice (Echinochloa colona) from California. |
Fecha de publicación : |
2015 |
Fuente / Imprenta : |
Pest Management Science, v. 71, n. 7, p. 996-2007, 2015. |
ISSN : |
1526-4998. |
DOI : |
10.1002/ps.3878 |
Idioma : |
Inglés |
Notas : |
Article history: Received: 4 April 2014 Revised: 31 July 2014/Accepted article published: 13 August 2014 /Published online in Wiley Online Library: 5 September 2014 |
Contenido : |
Abstract:BACKGROUND: Echinochloa colona is an annual weed affecting field crops and orchards in California. An E. colona population
carrying a mutation in the EPSPS gene endowing resistance to glyphosate, the most widely used non-selective herbicide,
was recently identified in the Northern Sacramento Valley of California. Plants from this population, from a suspected
glyphosate-resistant (GR) population, and from one susceptible (S) population collected in the Northern Sacramento Valley of
California, were used to generate three GR and one S selfedlines to study possiblemechanismsinvolved in glyphosate resistance.
RESULTS: Based on the amount of glyphosate required to kill 50% of the plants (LD50), GR lines were 4?9-fold more resistant than
S plants and accumulated less shikimate after glyphosate treatment. GR and S lines did not differ in glyphosate absorption,
translocation or metabolism. A different target-site mutation was found in each of two of the GR lines corresponding to
Pro106Thr and Pro106Ser substitutions; the mutations were found in different homoeologous EPSPS genes. No mutation was
found in the third GR line, which exhibited 1.4-fold higher basal EPSPS activity and a fivefold greater LD50 than S plants.
Quantitative RT-PCR revealed that GR lines had similar or lower EPSPS expression than S plants.
CONCLUSION: It is demonstrated that individuals with different glyphosate resistance mechanisms can coexist in the same
population, individuals from different populations may carry different resistance mechanisms and different mechanisms can
act in concert within single E. colona plants. However, other plant factors or resistance mechanisms appear to modulate plant
expression of EPSPS sensitivity to glyphosate. MenosAbstract:BACKGROUND: Echinochloa colona is an annual weed affecting field crops and orchards in California. An E. colona population
carrying a mutation in the EPSPS gene endowing resistance to glyphosate, the most widely used non-selective herbicide,
was recently identified in the Northern Sacramento Valley of California. Plants from this population, from a suspected
glyphosate-resistant (GR) population, and from one susceptible (S) population collected in the Northern Sacramento Valley of
California, were used to generate three GR and one S selfedlines to study possiblemechanismsinvolved in glyphosate resistance.
RESULTS: Based on the amount of glyphosate required to kill 50% of the plants (LD50), GR lines were 4?9-fold more resistant than
S plants and accumulated less shikimate after glyphosate treatment. GR and S lines did not differ in glyphosate absorption,
translocation or metabolism. A different target-site mutation was found in each of two of the GR lines corresponding to
Pro106Thr and Pro106Ser substitutions; the mutations were found in different homoeologous EPSPS genes. No mutation was
found in the third GR line, which exhibited 1.4-fold higher basal EPSPS activity and a fivefold greater LD50 than S plants.
Quantitative RT-PCR revealed that GR lines had similar or lower EPSPS expression than S plants.
CONCLUSION: It is demonstrated that individuals with different glyphosate resistance mechanisms can coexist in the same
population, individuals from different popula... Presentar Todo |
Palabras claves : |
5-ENOLPYRUVYLSHIKIMATE-3-PHOSPHATE SYNTHASE; EPSPS EXPRESSION; EPSPS INHIBITION ASSAY; POLYPLOIDY; RESISTANCE MECHANISM. |
Thesagro : |
RESISTENCIA A GLIFOSATO. |
Asunto categoría : |
H60 Malezas y escardas |
Marc : |
LEADER 02883naa a2200313 a 4500 001 1050961 005 2019-11-05 008 2015 bl uuuu u00u1 u #d 022 $a1526-4998. 024 7 $a10.1002/ps.3878$2DOI 100 1 $aALARCÓN-REVERTE, R. 245 $aConcerted action of target-site mutations and high EPSPS activity in glyphosate-resistant junglerice (Echinochloa colona) from California.$h[electronic resource] 260 $c2015 500 $aArticle history: Received: 4 April 2014 Revised: 31 July 2014/Accepted article published: 13 August 2014 /Published online in Wiley Online Library: 5 September 2014 520 $aAbstract:BACKGROUND: Echinochloa colona is an annual weed affecting field crops and orchards in California. An E. colona population carrying a mutation in the EPSPS gene endowing resistance to glyphosate, the most widely used non-selective herbicide, was recently identified in the Northern Sacramento Valley of California. Plants from this population, from a suspected glyphosate-resistant (GR) population, and from one susceptible (S) population collected in the Northern Sacramento Valley of California, were used to generate three GR and one S selfedlines to study possiblemechanismsinvolved in glyphosate resistance. RESULTS: Based on the amount of glyphosate required to kill 50% of the plants (LD50), GR lines were 4?9-fold more resistant than S plants and accumulated less shikimate after glyphosate treatment. GR and S lines did not differ in glyphosate absorption, translocation or metabolism. A different target-site mutation was found in each of two of the GR lines corresponding to Pro106Thr and Pro106Ser substitutions; the mutations were found in different homoeologous EPSPS genes. No mutation was found in the third GR line, which exhibited 1.4-fold higher basal EPSPS activity and a fivefold greater LD50 than S plants. Quantitative RT-PCR revealed that GR lines had similar or lower EPSPS expression than S plants. CONCLUSION: It is demonstrated that individuals with different glyphosate resistance mechanisms can coexist in the same population, individuals from different populations may carry different resistance mechanisms and different mechanisms can act in concert within single E. colona plants. However, other plant factors or resistance mechanisms appear to modulate plant expression of EPSPS sensitivity to glyphosate. 650 $aRESISTENCIA A GLIFOSATO 653 $a5-ENOLPYRUVYLSHIKIMATE-3-PHOSPHATE SYNTHASE 653 $aEPSPS EXPRESSION 653 $aEPSPS INHIBITION ASSAY 653 $aPOLYPLOIDY 653 $aRESISTANCE MECHANISM 700 1 $aGARCIA, M.A. 700 1 $aWATSON, S.B. 700 1 $aABDALLAH, I. 700 1 $aSABATÉ, S. 700 1 $aHERNÁNDEZ, M.J. 700 1 $aDAYAN ,F.E. 700 1 $aFISCHER, A.J. 773 $tPest Management Science$gv. 71, n. 7, p. 996-2007, 2015.
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Registros recuperados : 2 | |
1. | | SINCHI, F. I.; ZUIN, J. F.; GARZÓN, J. P.; LÓPEZ, G. E.; CALLE, G. R.; QUITO, F.; GALARZA, D. A.; PEREA, F. P. Addition of clinoptilolite in the diet reduces uterine PMN leukocytes and open days in multiparous lactating dairy cows managed in a mountain tropical pasture-based system. Tropical Animal Health and Production, 2022, Volume 54, Issue 5, Article 281. doi: https://doi.org/10.1007/s11250-022-03308-2 Article history: Received 17 June 2021; Accepted 31 August 2022; Published online 08 September 2022.
Corresponding author: Perea, F.P.; Carrera de Medicina Veterinaria y Zootecnia, Facultad de Ciencias Agropecuarias, Universidad de...Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
Biblioteca(s): INIA Las Brujas. |
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2. | | ESCANDÓN, B.M.; ESPINOZA, J.S.; PEREA, F.P.; FERNANDO QUITO, F.; OCHOA, R.; LÓPEZ, G.E.; GALARZA, D.A.; GARZÓN, J. P. Intrauterine therapy with ozone reduces subclinical endometritis and improves reproductive performance in postpartum dairy cows managed in pasture-based systems. Tropical Animal Health Production,2020. DOI: https://doi.org/10.1007/s11250-020-02298-3 Article history: Received06 December 2019// Accepted13 May 2020// Published22 May 2020. The research was supported by Dirección de Investigación de la Universidad de Cuenca (DIUC), Ecuador, throughout project DIUC-XIV-2016-022.The authors...Biblioteca(s): INIA La Estanzuela. |
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Registros recuperados : 2 | |
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