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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
26/09/2014 |
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Actualizado : |
18/03/2022 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
IBÁÑEZ, F.; REZANO, A.; FREDES, A.; RODRIGUEZ, G.; REGGIO, A.; VILARÓ, F. |
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Afiliación : |
FACUNDO IBÁÑEZ SILVA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ANABELA REZANO MATKOVICH, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; ALEJANDRO DAVID FREDES SIVOPLAS, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; GUSTAVO ADOLFO RODRIGUEZ TEXEIRA, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; ADRIANA MARIA REGGIO BIGANZOLI, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; FRANCISCO LUIS VILARO PAREJA, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. |
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Título : |
Development of a rapid microplate assay for determining pungency in onion breeding program. |
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Fecha de publicación : |
2012 |
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Fuente / Imprenta : |
Acta Horticulturae (ISHS), 2012, no. 935, p. 61-65. |
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ISSN : |
0567-7572 |
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DOI : |
10.17660/ActaHortic.2012.935.8 |
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Idioma : |
Inglés |
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Notas : |
XXVIII International Horticultural Congress on Science and Horticulture for People (IHC2010): International Symposium on New Developments in Plant Genetics and Breeding. |
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Contenido : |
ABSTRACT.
Onions with low levels of pungency and sweet flavor are becoming popular with consumers on an international level. The trend of greater demand in the international market will continue as sweet onions are introduced and promoted as a specialty item. Large-scale screening of onion bulbs for pungency requires a fast and cost-efficient method. The original method which was proposed by Schwimmer and Weston (1961), it is slow and tedious when large quantity of samples is analyzed in breeding programs. Moreover, it involves the use of dinitrophenylhydrazine, a chemical difficult to handle and very toxic. In our breeding program for developing low pungency cultivars, the screening is performed for more than 3000 bulbs annually. A 96-well microplate procedure for pyruvic acid analysis was developed in order to screen a large number of onion bulbs in our breeding rogram. The new method is based on the selective reaction of p-dimethylaminobenzaldehyde with enzymatically produced pyruvic acid. Analysis conditions were optimized with Response Surface Modelling using a Box-Behnken Design. With this method it is possible to determine the pyruvic acid content in 96 onion bulbs in just a few minutes. In a test using our cultivars, this method showed high correlations with spectrophotometric method (R2>0.95). |
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Thesagro : |
AJO. |
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Asunto categoría : |
-- |
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Marc : |
LEADER 02138naa a2200229 a 4500
001 1050611
005 2022-03-18
008 2012 bl uuuu u00u1 u #d
022 $a0567-7572
024 7 $a10.17660/ActaHortic.2012.935.8$2DOI
100 1 $aIBÁÑEZ, F.
245 $aDevelopment of a rapid microplate assay for determining pungency in onion breeding program.$h[electronic resource]
260 $c2012
500 $aXXVIII International Horticultural Congress on Science and Horticulture for People (IHC2010): International Symposium on New Developments in Plant Genetics and Breeding.
520 $aABSTRACT. Onions with low levels of pungency and sweet flavor are becoming popular with consumers on an international level. The trend of greater demand in the international market will continue as sweet onions are introduced and promoted as a specialty item. Large-scale screening of onion bulbs for pungency requires a fast and cost-efficient method. The original method which was proposed by Schwimmer and Weston (1961), it is slow and tedious when large quantity of samples is analyzed in breeding programs. Moreover, it involves the use of dinitrophenylhydrazine, a chemical difficult to handle and very toxic. In our breeding program for developing low pungency cultivars, the screening is performed for more than 3000 bulbs annually. A 96-well microplate procedure for pyruvic acid analysis was developed in order to screen a large number of onion bulbs in our breeding rogram. The new method is based on the selective reaction of p-dimethylaminobenzaldehyde with enzymatically produced pyruvic acid. Analysis conditions were optimized with Response Surface Modelling using a Box-Behnken Design. With this method it is possible to determine the pyruvic acid content in 96 onion bulbs in just a few minutes. In a test using our cultivars, this method showed high correlations with spectrophotometric method (R2>0.95).
650 $aAJO
700 1 $aREZANO, A.
700 1 $aFREDES, A.
700 1 $aRODRIGUEZ, G.
700 1 $aREGGIO, A.
700 1 $aVILARÓ, F.
773 $tActa Horticulturae (ISHS), 2012, no. 935, p. 61-65.
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Registro original : |
INIA Las Brujas (LB) |
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| Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha actual : |
03/12/2019 |
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Actualizado : |
13/02/2020 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
BALDASSINI, P.; PARUELO, J.M. |
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Afiliación : |
IFEVA and Departamento de Métodos Cuantitativos y Sistemas de Información,Facultad de Agronomía,Universidad de Buenos Aires and CONICET ,Buenos Aires, Argentina; JOSÉ PARUELO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay./IFEVA and Departamento de Métodos Cuantitativos y Sistemas de Información,Facultad de Agronomía,Universidad de Buenos Aires and CONICET,Argentina./IECA,Facultad de Ciencias, Argentina. |
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Título : |
Deforestation and current management practices reduce soil organic carbon in the semi-arid Chaco, Argentina. |
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Fecha de publicación : |
2020 |
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Fuente / Imprenta : |
Agricultural Systems, February 2020, Volume 178, Article number 102749. DOI: https://doi.org/10.1016/j.agsy.2019.102749 |
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DOI : |
10.1016/j.agsy.2019.102749 |
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Idioma : |
Inglés |
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Notas : |
Article history:Received 10 April2019/ Received in revised form 1 October 2019/Accepted 12 November 2019. Corresponding author:
E-mail addresses: pbaldass@agro.uba.ar (P. Baldassini), jparuelo@inia.org.uy (J.M. Paruelo).Acknowledgments: This work was carried out with the aid of a grant from the InterAmerican Institute for Global Change Research (IAI) CRN3095, which is supported by the US National Science Foundation (Grant GEO1128040). This research was also supported by UBA, CONICET and FONCyT-PICT 2199. We thank C. Bagnato, S. Aguiar, L. Amdan, L
Ciuffoli for their assistance with field work and samples processing. We thank Agropecuaria Andorrana and Anta del Dorado for allowing us
carry our fieldwork in their farms. We are grateful to F. Mónico and the staff of INTA AER J.V. Gonzalez for their logistic support. |
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Palabras claves : |
CENTURY; HANPP; LAND USE CHANGE; NET PRIMARY PRODUCTION; SOIL ORGANIC CARBON. |
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Thesagro : |
ARGENTINA; DEFORESTACION. |
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Asunto categoría : |
-- |
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Marc : |
LEADER 01587naa a2200229 a 4500
001 1060495
005 2020-02-13
008 2020 bl uuuu u00u1 u #d
024 7 $a10.1016/j.agsy.2019.102749$2DOI
100 1 $aBALDASSINI, P.
245 $aDeforestation and current management practices reduce soil organic carbon in the semi-arid Chaco, Argentina.$h[electronic resource]
260 $c2020
500 $aArticle history:Received 10 April2019/ Received in revised form 1 October 2019/Accepted 12 November 2019. Corresponding author: E-mail addresses: pbaldass@agro.uba.ar (P. Baldassini), jparuelo@inia.org.uy (J.M. Paruelo).Acknowledgments: This work was carried out with the aid of a grant from the InterAmerican Institute for Global Change Research (IAI) CRN3095, which is supported by the US National Science Foundation (Grant GEO1128040). This research was also supported by UBA, CONICET and FONCyT-PICT 2199. We thank C. Bagnato, S. Aguiar, L. Amdan, L Ciuffoli for their assistance with field work and samples processing. We thank Agropecuaria Andorrana and Anta del Dorado for allowing us carry our fieldwork in their farms. We are grateful to F. Mónico and the staff of INTA AER J.V. Gonzalez for their logistic support.
650 $aARGENTINA
650 $aDEFORESTACION
653 $aCENTURY
653 $aHANPP
653 $aLAND USE CHANGE
653 $aNET PRIMARY PRODUCTION
653 $aSOIL ORGANIC CARBON
700 1 $aPARUELO, J.M.
773 $tAgricultural Systems, February 2020, Volume 178, Article number 102749. DOI: https://doi.org/10.1016/j.agsy.2019.102749
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