|
|
Registro completo
|
Biblioteca (s) : |
INIA La Estanzuela. |
Fecha : |
19/09/2014 |
Actualizado : |
23/10/2019 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
ALEM, D.; NARANCIO, R.; DIAZ, P.; REBUFFO, M.; ZARZA, R.; DALLA RIZZA, M. |
Afiliación : |
DIEGO MARTIN ALEM GLISON, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; RAFAEL NARANCIO FERES, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; PAOLA ALEXANDRA DIAZ DELLAVALLE, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; MONICA IRENE REBUFFO GFELLER, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; RODRIGO TABARE ZARZA FUENTES, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; MARCO DALLA RIZZA VILARO, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. |
Título : |
Molecular characterization of Lotus corniculatus cultivars using transferable microsatellite markers. |
Fecha de publicación : |
2011 |
Fuente / Imprenta : |
Ciencia e Investigación Agraria, Santiago de Chile, v. 38, n. 3, p. 463-471, 2011. |
ISSN : |
0304-5609 |
DOI : |
10.4067/S0718-16202011000300015 |
Idioma : |
Inglés |
Notas : |
Article history: Received September 7 2010. /Accepted June 21, 2011. |
Contenido : |
Abstract :Lotus corniculatus L. is the most important agricultural species in the genus Lotus and is the most widely distributed Lotus species worldwide. L. corniculatus genotypes form complex groups that are difficult to recognize both morphologically and biochemically. Given the extensive and expensive process of isolating Simple Sequence Repeats (SSR, also called microsatellites), the possibility of using microsatellites already identified in related species is highly attractive. The aim of this work was the identification and validation of transferable microsatellite markers in L. corniculatus, and using those markers to study the genetic variability among four cultivars. Each cultivar of L. corniculatus was represented by 15 genotypes. Ten microsatellite markers were evaluated, and from those, four were selected based on their discriminative values observed among cultivars. We detected 29 alleles for the four markers, and there was an average of 7.25 alleles per locus. The marker TM0197 had the fewest number of alleles (5) and TM0083 had the highest number of alleles (10). The polymorphic information content (PIC) for the selected markers varied from 0.19 to 0.35, and the markers were therefore classified as highly informative. Based on the markers, we found high variability between individuals of the same cultivar. The use of transferable microsatellite markers could be useful to differentiate individuals at a relatively low cost, showing a great potential for use in breeding programs. MenosAbstract :Lotus corniculatus L. is the most important agricultural species in the genus Lotus and is the most widely distributed Lotus species worldwide. L. corniculatus genotypes form complex groups that are difficult to recognize both morphologically and biochemically. Given the extensive and expensive process of isolating Simple Sequence Repeats (SSR, also called microsatellites), the possibility of using microsatellites already identified in related species is highly attractive. The aim of this work was the identification and validation of transferable microsatellite markers in L. corniculatus, and using those markers to study the genetic variability among four cultivars. Each cultivar of L. corniculatus was represented by 15 genotypes. Ten microsatellite markers were evaluated, and from those, four were selected based on their discriminative values observed among cultivars. We detected 29 alleles for the four markers, and there was an average of 7.25 alleles per locus. The marker TM0197 had the fewest number of alleles (5) and TM0083 had the highest number of alleles (10). The polymorphic information content (PIC) for the selected markers varied from 0.19 to 0.35, and the markers were therefore classified as highly informative. Based on the markers, we found high variability between individuals of the same cultivar. The use of transferable microsatellite markers could be useful to differentiate individuals at a relatively low cost, showing a great potential for use in b... Presentar Todo |
Palabras claves : |
GENETIC VARIABILITY; LOTUS JAPONICUS; MICROSATELLITES; SIMPLE SEQUENCE REPEATS; SSR; VARIABILIDAD GENÉTICA. |
Thesagro : |
CULTIVOS. |
Asunto categoría : |
F01 Cultivo |
URL : |
http://www.scielo.cl/pdf/ciagr/v38n3/art15.pdf
|
Marc : |
LEADER 02470naa a2200301 a 4500 001 1050403 005 2019-10-23 008 2011 bl uuuu u00u1 u #d 022 $a0304-5609 024 7 $a10.4067/S0718-16202011000300015$2DOI 100 1 $aALEM, D. 245 $aMolecular characterization of Lotus corniculatus cultivars using transferable microsatellite markers.$h[electronic resource] 260 $c2011 500 $aArticle history: Received September 7 2010. /Accepted June 21, 2011. 520 $aAbstract :Lotus corniculatus L. is the most important agricultural species in the genus Lotus and is the most widely distributed Lotus species worldwide. L. corniculatus genotypes form complex groups that are difficult to recognize both morphologically and biochemically. Given the extensive and expensive process of isolating Simple Sequence Repeats (SSR, also called microsatellites), the possibility of using microsatellites already identified in related species is highly attractive. The aim of this work was the identification and validation of transferable microsatellite markers in L. corniculatus, and using those markers to study the genetic variability among four cultivars. Each cultivar of L. corniculatus was represented by 15 genotypes. Ten microsatellite markers were evaluated, and from those, four were selected based on their discriminative values observed among cultivars. We detected 29 alleles for the four markers, and there was an average of 7.25 alleles per locus. The marker TM0197 had the fewest number of alleles (5) and TM0083 had the highest number of alleles (10). The polymorphic information content (PIC) for the selected markers varied from 0.19 to 0.35, and the markers were therefore classified as highly informative. Based on the markers, we found high variability between individuals of the same cultivar. The use of transferable microsatellite markers could be useful to differentiate individuals at a relatively low cost, showing a great potential for use in breeding programs. 650 $aCULTIVOS 653 $aGENETIC VARIABILITY 653 $aLOTUS JAPONICUS 653 $aMICROSATELLITES 653 $aSIMPLE SEQUENCE REPEATS 653 $aSSR 653 $aVARIABILIDAD GENÉTICA 700 1 $aNARANCIO, R. 700 1 $aDIAZ, P. 700 1 $aREBUFFO, M. 700 1 $aZARZA, R. 700 1 $aDALLA RIZZA, M. 773 $tCiencia e Investigación Agraria, Santiago de Chile$gv. 38, n. 3, p. 463-471, 2011.
Descargar
Esconder MarcPresentar Marc Completo |
Registro original : |
INIA La Estanzuela (LE) |
|
Biblioteca
|
Identificación
|
Origen
|
Tipo / Formato
|
Clasificación
|
Cutter
|
Registro
|
Volumen
|
Estado
|
Volver
|
|
Registro completo
|
Biblioteca (s) : |
INIA Las Brujas; INIA Tacuarembó; INIA Treinta y Tres. |
Fecha actual : |
21/02/2014 |
Actualizado : |
22/02/2014 |
Autor : |
García, S.Paullier, J.Pagani, C. |
Afiliación : |
INIA Las Brujas |
Título : |
Control de enfermedades y plagas en cebolla y ajo |
Fecha de publicación : |
1997 |
Fuente / Imprenta : |
Treinta y Tres (Uruguay): INIA. Programa Nacional de Horticultura, 1997. |
Páginas : |
p. 28-31 |
Serie : |
(INIA Serie Actividades de Difusión ; 126) |
Idioma : |
Español |
Notas : |
INIA Treinta y Tres |
Thesagro : |
AJO; ALLIUM CEPA; ALLIUM SATIVUM; CEBOLLA; CONTROL DE ENFERMEDADES; CONTROL DE PLAGAS. |
Asunto categoría : |
-- |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/260/1/18429180808151559.pdf
|
Marc : |
LEADER 00646naa a2200217 a 4500 001 1007551 005 2014-02-22 008 1997 bl uuuu u00u1 u #d 100 1 $aGARCÍA, S.PAULLIER, J.PAGANI, C. 245 $aControl de enfermedades y plagas en cebolla y ajo 260 $c1997 300 $ap. 28-31 490 $a(INIA Serie Actividades de Difusión ; 126) 500 $aINIA Treinta y Tres 650 $aAJO 650 $aALLIUM CEPA 650 $aALLIUM SATIVUM 650 $aCEBOLLA 650 $aCONTROL DE ENFERMEDADES 650 $aCONTROL DE PLAGAS 773 $tTreinta y Tres (Uruguay): INIA. Programa Nacional de Horticultura, 1997.
Descargar
Esconder MarcPresentar Marc Completo |
Registro original : |
INIA Las Brujas (LB) |
|
No copia registrada de este documento.
|
Volver
|
Expresión de búsqueda válido. Check! |
|
|