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Biblioteca (s) : |
INIA Treinta y Tres. |
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Fecha : |
22/12/2020 |
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Actualizado : |
11/03/2021 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
CASTILLO, A.; LOPEZ, V.; TAVARES, E.; SANTIÑAQUE, F.; DALLA RIZZA, M. |
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Afiliación : |
ALICIA MARIA CASTILLO SALLE, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; VICTOR JULIAN LOPEZ DEL PUERTO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; E. TAVARES, Forestal Oriental, Paysandú Uruguay.; F. SANTIÑAQUE, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay.; MARCO DALLA RIZZA VILARO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Polyploid induction of Eucalyptus dunnii Maiden to generate waviability in breeding programs. [Polyploid induction of Eucalyptus dunnii Maiden to generate variability in breeding programs]. |
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Complemento del título : |
Special Issue X Encuentro Latinoamericano y del Caribe de Biotecnología Agropecuaria; XII Simposio REDBIO Argentina. Roca, William, Ed. 12 al 15 de noviembre de 2019, Montevideo, Uruguay. |
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Fecha de publicación : |
2020 |
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Fuente / Imprenta : |
Agrociencia Uruguay 2020, v. 24, no. NE2, Article 381. DOI: 10.31285/AGRO.24.413 |
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Páginas : |
9 p. |
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ISSN : |
2301-1548 |
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DOI : |
10.31285/AGRO.24.381 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 29 Jun. 2020 // Accepted 28 Sep. 2020 // Published 17 Dec 2020. Comité científico editor: Dra. Marisa López-Bilbao (INTA, Hurlingham, Provincia de Buenos Aires, Argentina); Dra. Sandra Sharry (Universidad Nacional de la Plata, La Plata, Buenos Aires, Argentina); Dra. Alicia Castillo (Investigadora Principal, Unidad de Biotecnología de INIA, Uruguay) ; Dr. Juan Izquierdo (Profesor Libre Facultad de Agronomía, Udelar, Presidente Academia Chilena Ciencias Agronómicas) ; Dr. Gerardo Gallego (Coordinador del Laboratorio de Biotecnología Vegetal del Centro Internacional de Agricultura Tropical-CIAT Cali, Colombia) ; Dra. Elizabeth Hodson (Profesora emérita de la Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá, Colombia); Dr. Paul Chavarriaga (líder de la plataforma de transformación genética y edición de genomas del Centro Internacional de Agricultura Tropical-CIAT Cali, Colombia) ; Dr. Marco Dalla-Rizza (Coordinador de la Unidad de Biotecnología, Investigador principal referente, presidente REDBIO 2016-2020). |
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Contenido : |
Eucalyptus dunnii Maiden produces good quality cellulose pulp, showing good frost tolerance. However, in Uru-guay, it needs more than six years to reach the reproductive stage. Genome duplication was proposed as a strategy to obtain useful variability. The application of mitosis inhibitors for chromosomal duplication, in explants growing in vitro, was evaluated. Two antimitotic agents were used: colchicine and oryzalin in different concen-trations and exposure times, in two types of explants: explants growing in vitro and pre-germinated seeds. The number of chloroplasts was used as a rapid ploidy estimator and confirmed by flow cytometry. For chloroplast count, fluorescein diacetate (FDA) applied to in vitro leaves was used for staining. Oryzalin was effective for inducing plant duplication in E. dunnii from in vitro explants. In pre-germinated seeds, both antimitotic agents induced polyploids. The average number of chloroplasts was 5.5 in diploid control plants and more than 7 in tetraploids. Obtained plantlets were successfully cloned in the greenhouse. This is the first report on artificial polyploidy obtained in E. dunnii. |
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Palabras claves : |
COLCHICINA; COLCHICINE; CULTIVO IN VITRO; EUCALIPTO; IN VITRO CULTURE; ORYZALIN; ORYZALINE; POLIPLOIDIA; POLYPLOIDY. |
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Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/14914/1/Castillo-Agrociencia-2020.pdf
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Marc : |
LEADER 03227naa a2200325 a 4500
001 1061636
005 2021-03-11
008 2020 bl uuuu u00u1 u #d
022 $a2301-1548
024 7 $a10.31285/AGRO.24.381$2DOI
100 1 $aCASTILLO, A.
245 $aPolyploid induction of Eucalyptus dunnii Maiden to generate waviability in breeding programs. [Polyploid induction of Eucalyptus dunnii Maiden to generate variability in breeding programs].$h[electronic resource]
260 $c2020
300 $a9 p.
500 $aArticle history: Received 29 Jun. 2020 // Accepted 28 Sep. 2020 // Published 17 Dec 2020. Comité científico editor: Dra. Marisa López-Bilbao (INTA, Hurlingham, Provincia de Buenos Aires, Argentina); Dra. Sandra Sharry (Universidad Nacional de la Plata, La Plata, Buenos Aires, Argentina); Dra. Alicia Castillo (Investigadora Principal, Unidad de Biotecnología de INIA, Uruguay) ; Dr. Juan Izquierdo (Profesor Libre Facultad de Agronomía, Udelar, Presidente Academia Chilena Ciencias Agronómicas) ; Dr. Gerardo Gallego (Coordinador del Laboratorio de Biotecnología Vegetal del Centro Internacional de Agricultura Tropical-CIAT Cali, Colombia) ; Dra. Elizabeth Hodson (Profesora emérita de la Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá, Colombia); Dr. Paul Chavarriaga (líder de la plataforma de transformación genética y edición de genomas del Centro Internacional de Agricultura Tropical-CIAT Cali, Colombia) ; Dr. Marco Dalla-Rizza (Coordinador de la Unidad de Biotecnología, Investigador principal referente, presidente REDBIO 2016-2020).
520 $aEucalyptus dunnii Maiden produces good quality cellulose pulp, showing good frost tolerance. However, in Uru-guay, it needs more than six years to reach the reproductive stage. Genome duplication was proposed as a strategy to obtain useful variability. The application of mitosis inhibitors for chromosomal duplication, in explants growing in vitro, was evaluated. Two antimitotic agents were used: colchicine and oryzalin in different concen-trations and exposure times, in two types of explants: explants growing in vitro and pre-germinated seeds. The number of chloroplasts was used as a rapid ploidy estimator and confirmed by flow cytometry. For chloroplast count, fluorescein diacetate (FDA) applied to in vitro leaves was used for staining. Oryzalin was effective for inducing plant duplication in E. dunnii from in vitro explants. In pre-germinated seeds, both antimitotic agents induced polyploids. The average number of chloroplasts was 5.5 in diploid control plants and more than 7 in tetraploids. Obtained plantlets were successfully cloned in the greenhouse. This is the first report on artificial polyploidy obtained in E. dunnii.
653 $aCOLCHICINA
653 $aCOLCHICINE
653 $aCULTIVO IN VITRO
653 $aEUCALIPTO
653 $aIN VITRO CULTURE
653 $aORYZALIN
653 $aORYZALINE
653 $aPOLIPLOIDIA
653 $aPOLYPLOIDY
700 1 $aLOPEZ, V.
700 1 $aTAVARES, E.
700 1 $aSANTIÑAQUE, F.
700 1 $aDALLA RIZZA, M.
773 $tAgrociencia Uruguay 2020$gv. 24, no. NE2, Article 381. DOI: 10.31285/AGRO.24.413
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Registro original : |
INIA Treinta y Tres (TT) |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
11/03/2024 |
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Actualizado : |
11/03/2024 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
FARMAN, M.L.; ASCARI, J.P.; RAHNAMA, M.; PONTE, E.M DEL; PEDLEY, K.F.; MARTÍNEZ, S.; FERNANDES, J.M.C.; VALENT, B. |
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Afiliación : |
MARK L. FARMAN, Department of Plant Pathology, University of Kentucky, Lexington, United States; JOAO P. ASCARI, Departamento de Fitopatologia, Universidade Federal de Viçosa, MG, Viçosa, 36570-900, Brazil; MOSTAFA RAHNAMA, Department of Plant Pathology, University of Kentucky, Lexington, United States; EMERSON M DEL PONTE, Departamento de Fitopatologia, Universidade Federal de Viçosa, MG, Viçosa, 36570-900, Brazil; KERRY F. PEDLEY, U.S. Department of Agriculture-Agricultural Research Service, Foreign Disease Weed Science Research Unit, Fort Detrick, MD 21702, United States; SEBASTIÁN MARTÍNEZ KOPP, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JOSÉ MAURÍCIO C. FERNANDES, Embrapa Trigo, RS, Passo Fundo, 99050-970, Brazil; BARBARA VALENT, Department of Plant Pathology, Kansas State University, Manhattan, KS 66506, United States. |
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Título : |
A reevaluation of phylogenomic data reveals that current understanding in wheat blast population biology and epidemiology is obfuscated by oversights in population sampling. |
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Complemento del título : |
Population biology. |
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Fecha de publicación : |
2024 |
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Fuente / Imprenta : |
Phytopathology. 2024, 114(1): 220-225. https://doi.org/10.1094/PHYTO-01-23-0025-R |
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ISSN : |
0031-949X |
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DOI : |
10.1094/PHYTO-01-23-0025-R |
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Idioma : |
Inglés |
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Notas : |
Article history: Accepted for publication 10 July 2023; Published online 23 December 2023. -- Corresponding author: M. L. Farman; farman@uky.edu . -- Document type: Article Green Open Access. -- Funding:Support was provided by the U.S. Department of Agriculture, Agriculture and Food Research Initiative (grant 2013-68004-20378, multistate project NE1602); Agricultural Research Service (project 8044-22000-046-00D and Hatchproject KY012037); the National Science Foundation (grant MCB-1716491); the University of Kentucky College of Agriculture Food and the Environment; Fundaçãode Amparo à Pesquisa do Estado de Minas Gerais (grant APQ-03072-18 to E. M. DelPonte); Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (PROEX);and National Council for Scientific and Technological Development (CNPq) (grant310208/2019-0). E. M. Del Ponte was supported by CNPq through a Productivity Research Fellowship (project 310208/2019-0). J. P. Ascari was supported by CNPq through a doctoral scholarship. -- Supplementary material is available online: |
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Contenido : |
ABSTRACT.- Wheat blast, caused by the Pyricularia oryzae Triticum lineage (PoT), first emerged in Brazil and quickly spread to neighboring countries. Its recent appearance in Bangladesh and Zambia highlights a need to understand the disease's population biology and epidemiology so as to mitigate pandemic outbreaks. Current knowledge is mostly based on characterizations of Brazilian wheat blast isolates and comparison with isolates from non-wheat, endemic grasses. These foregoing studies concluded that the wheat blast population lacks host specificity and, as a result, undergoes extensive gene flow with populations infecting non-wheat hosts. Additionally, based on genetic similarity between wheat blast and isolates infecting Urochloa species, it was proposed that the disease originally emerged via a host jump from this grass and that Urochloa likely plays a central role in wheat blast epidemiology owing to its widespread use as a pasture grass. However, due to inconsistencies with broader phylogenetic studies, we suspected that these seminal studies had not actually sampled the populations normally found on endemic grasses and, instead, had repeatedly isolated members of PoT and the related Lolium pathogen lineage (PoL1). Re-analysis of the Brazilian data as part of a comprehensive, global, phylogenomic dataset that included a small number of South American isolates sampled away from wheat confirmed our suspicion and identified four new P. oryzae lineages on grass hosts. As a result, the conclusions underpinning current understanding in wheat blast's evolution, population biology, and epidemiology are unsubstantiated and could be equivocal. @ This article is in the public domain and not copyrightable. It may be freely reprinted with customary crediting of the source. The American Phytopathological Society, 2023. MenosABSTRACT.- Wheat blast, caused by the Pyricularia oryzae Triticum lineage (PoT), first emerged in Brazil and quickly spread to neighboring countries. Its recent appearance in Bangladesh and Zambia highlights a need to understand the disease's population biology and epidemiology so as to mitigate pandemic outbreaks. Current knowledge is mostly based on characterizations of Brazilian wheat blast isolates and comparison with isolates from non-wheat, endemic grasses. These foregoing studies concluded that the wheat blast population lacks host specificity and, as a result, undergoes extensive gene flow with populations infecting non-wheat hosts. Additionally, based on genetic similarity between wheat blast and isolates infecting Urochloa species, it was proposed that the disease originally emerged via a host jump from this grass and that Urochloa likely plays a central role in wheat blast epidemiology owing to its widespread use as a pasture grass. However, due to inconsistencies with broader phylogenetic studies, we suspected that these seminal studies had not actually sampled the populations normally found on endemic grasses and, instead, had repeatedly isolated members of PoT and the related Lolium pathogen lineage (PoL1). Re-analysis of the Brazilian data as part of a comprehensive, global, phylogenomic dataset that included a small number of South American isolates sampled away from wheat confirmed our suspicion and identified four new P. oryzae lineages on grass hosts. As a... Presentar Todo |
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Palabras claves : |
Magnaporthe oryzae; Phylogenetics. |
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Asunto categoría : |
F01 Cultivo |
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URL : |
https://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO-01-23-0025-R
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Marc : |
LEADER 03766naa a2200265 a 4500
001 1064501
005 2024-03-11
008 2024 bl uuuu u00u1 u #d
022 $a0031-949X
024 7 $a10.1094/PHYTO-01-23-0025-R$2DOI
100 1 $aFARMAN, M.L.
245 $aA reevaluation of phylogenomic data reveals that current understanding in wheat blast population biology and epidemiology is obfuscated by oversights in population sampling.$h[electronic resource]
260 $c2024
500 $aArticle history: Accepted for publication 10 July 2023; Published online 23 December 2023. -- Corresponding author: M. L. Farman; farman@uky.edu . -- Document type: Article Green Open Access. -- Funding:Support was provided by the U.S. Department of Agriculture, Agriculture and Food Research Initiative (grant 2013-68004-20378, multistate project NE1602); Agricultural Research Service (project 8044-22000-046-00D and Hatchproject KY012037); the National Science Foundation (grant MCB-1716491); the University of Kentucky College of Agriculture Food and the Environment; Fundaçãode Amparo à Pesquisa do Estado de Minas Gerais (grant APQ-03072-18 to E. M. DelPonte); Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (PROEX);and National Council for Scientific and Technological Development (CNPq) (grant310208/2019-0). E. M. Del Ponte was supported by CNPq through a Productivity Research Fellowship (project 310208/2019-0). J. P. Ascari was supported by CNPq through a doctoral scholarship. -- Supplementary material is available online:
520 $aABSTRACT.- Wheat blast, caused by the Pyricularia oryzae Triticum lineage (PoT), first emerged in Brazil and quickly spread to neighboring countries. Its recent appearance in Bangladesh and Zambia highlights a need to understand the disease's population biology and epidemiology so as to mitigate pandemic outbreaks. Current knowledge is mostly based on characterizations of Brazilian wheat blast isolates and comparison with isolates from non-wheat, endemic grasses. These foregoing studies concluded that the wheat blast population lacks host specificity and, as a result, undergoes extensive gene flow with populations infecting non-wheat hosts. Additionally, based on genetic similarity between wheat blast and isolates infecting Urochloa species, it was proposed that the disease originally emerged via a host jump from this grass and that Urochloa likely plays a central role in wheat blast epidemiology owing to its widespread use as a pasture grass. However, due to inconsistencies with broader phylogenetic studies, we suspected that these seminal studies had not actually sampled the populations normally found on endemic grasses and, instead, had repeatedly isolated members of PoT and the related Lolium pathogen lineage (PoL1). Re-analysis of the Brazilian data as part of a comprehensive, global, phylogenomic dataset that included a small number of South American isolates sampled away from wheat confirmed our suspicion and identified four new P. oryzae lineages on grass hosts. As a result, the conclusions underpinning current understanding in wheat blast's evolution, population biology, and epidemiology are unsubstantiated and could be equivocal. @ This article is in the public domain and not copyrightable. It may be freely reprinted with customary crediting of the source. The American Phytopathological Society, 2023.
653 $aMagnaporthe oryzae
653 $aPhylogenetics
700 1 $aASCARI, J.P.
700 1 $aRAHNAMA, M.
700 1 $aPONTE, E.M DEL
700 1 $aPEDLEY, K.F.
700 1 $aMARTÍNEZ, S.
700 1 $aFERNANDES, J.M.C.
700 1 $aVALENT, B.
773 $tPhytopathology. 2024, 114(1): 220-225. https://doi.org/10.1094/PHYTO-01-23-0025-R
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