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| 5. |  | NÚÑEZ, A.; RUBIO, V.; MOREIRA, J. Accumulated effects of contrasting phosphorus balances in the evolution of soil available phosphorus. [abstract]. Theme 1 - Phosphorus forms, availability and cycling in soils. Oral presentation. In: Michelini, D.; Garaycochea, S. (Eds.). 7th Phosphorus in Soils and Plants Symposium (PSP7). "Towards a sustainable phosphorus utilization in agroecosystems." Book of abstracts. PSP7, 3-7 October 2022, Montevideo, Uruguay. p.19. Published By: The organizing committee of the 7th Symposium on Phosphorus in Soils and Plants (PSP7)- National Agricultural Research Institute and School of Agronomy, Universidad de la República, Uruguay.| Biblioteca(s): INIA Las Brujas. |
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| 7. | | RUBIO, V.; QUINCKE, A.; ERNST, O. Deep tillage and nitrogen do not remediate cumulative soil deterioration effects of continuous cropping. Soil Tillage, Conservation, and Management. Agronomy Journal, 2021, Volume 113, Issue 6, Pages 5584-5596. doi: https://doi.org/10.1002/agj2.20927 Article history: Received 19 July 2021; Accepted 12 October 2021; Published online 25 November 2021.
Corresponding author: Rubio, V.; Instituto Nacional de Investigación Agropecuaria (INIA), Programa de Producción y Sustentabilidad...| Biblioteca(s): INIA Las Brujas. |
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| 8. | | RUBIO, V.; CASTRO, M.; DIAZ, R. Efecto de la variabilidad climática en el rendimiento de trigo en Uruguay. [Poster]. In: German, S.; Quincke, M.; Vázquez, D.; Castro, M.; Pereyra, S.; Silva, P.; García, A. (Eds.). Seminario Internacional "1914-2014: Un siglo de mejoramiento de trigo en La Estanzuela". Montevideo (UY): INIA, 2018. p. 131. (INIA Serie Técnica; 241).| Biblioteca(s): INIA La Estanzuela. |
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| 9. | | RUBIO, V.; CASTRO, M.; DIAZ, R. Efecto de la variabilidad climática en el rendimiento de trigo en Uruguay. In: SEMINARIO INTERNACIONAL DE TRIGO, 2014, La Estanzuela, Colonia, UY. GERMÁN, S., et al. (Org.). 1914-2014, un siglo de mejoramiento de trigo en La Estanzuela: un valioso legado para el futuro: resúmenes; posters. La Estanzuela, Colonia, UY: INIA, 2014. p. 42.| Biblioteca(s): INIA La Estanzuela. |
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| 15. | | RUBIO, V.; SAWCHIK, J.; ERNST, O.; QUINCKE, A.; SIRI, G. Calibración/validacion del modelo AMG para simular la evolucion del stock de carbono orgánico en suelos de Uruguay. In: Congreso Uruguayo de Suelos, 1.; Encuentro de la Sociedad Uruguaya de Ciencias del Suelo, 6., 2014, Colonia del Sacramento, UY. Intensificando el conocimiento del suelo y medioambiente para producir más y mejor; presentaciones orales: propiedades físicas químicas biológicas. [Montevideo: SUCS], 2014. p. 31.| Biblioteca(s): INIA La Estanzuela. |
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| 16. | | FILIPPI, D.; SAWCHIK, J.; TIECHER, T.; RUBIO, V. Efecto de cultivos de cobertura de invierno y su época de quema sobre estabilidad de agregados, stock de carbono y productividad de la soja. Resumen: 661. In: Congreso Latinoamericano de Ciencias de Suelo, 22.; 2do Congreso Uruguayo de Suelos, 2; Encuentro de la SUCS, 10. Diversidad Productiva:
pilar del manejo sostenible de los suelos. Montevideo, Uruguay: SUCS; SLSC, 2019. [Presentación Oral] + [Resumen].| Biblioteca(s): INIA La Estanzuela. |
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| 18. | | DIAZ, R.; RUBIO, V.; GARCIA LAMOTHE, A.; QUINCKE, A. Efecto de la variabilidad climática en la producción de trigo y medidas de adaptación para reducir riesgos. In: Congreso Uruguayo de Suelos, 1.; Encuentro de la Sociedad Uruguaya de Ciencias del Suelo, 6., 2014, Colonia del Sacramento, UY. Intensificando el conocimiento del suelo y medioambiente para producir más y mejor: presentaciones orales; propiedades físicas químicas biológicas. [Montevideo: SUCS], 2014. p. 50.| Biblioteca(s): INIA La Estanzuela. |
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| Registros recuperados : 32 | |
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 | Acceso al texto completo restringido a Biblioteca INIA Treinta y Tres. Por información adicional contacte bibliott@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Treinta y Tres. |
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Fecha actual : |
26/09/2014 |
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Actualizado : |
11/10/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
A - 2 |
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Autor : |
ABREO, E.; MARTINEZ, S.; BETTUCCI, L.; LUPO, S. |
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Afiliación : |
SEBASTIAN MARTINEZ KOPP, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. |
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Título : |
Phaeomoniella chlamydospora and Phaeoacremonium ssp. in gravevines from Uruguay. |
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Fecha de publicación : |
2011 |
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Fuente / Imprenta : |
Phytopathologia Mediterranea. 2011. v.50 (Supplement), p.77-85. |
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ISSN : |
1593-2095 |
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DOI : |
10.14601/Phytopathol_Mediterr-8682 |
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Idioma : |
Inglés |
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Notas : |
Article history: accepted for publication: February 15, 2010; published 2011.
http://dx.doi.org/10.14601/Phytopathol_Mediterr-8682 |
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Contenido : |
Summary
Symptoms corresponding to esca and Petri diseases have been described in Uruguay as being associated with Phaeomoniella chlamydospora and Phaeoacremonium spp. Isolates of Phaeoacremonium spp. recovered from diseased grapevines were characterized and identified. Additionally, specific primers developed for Pa. chlamydospora and Phaeoacremonium spp. were evaluated for direct detection of these fungi in asymptomatic grapevine tissues. The bark was removed, and the trunk underneath was surface-disinfected.
Chips from symptomatic grapevines were plated on potato dextrose agar and incubated at 25°C. Isolates were identified morphologically and phylogenetically. Sequences of ITS rDNA, ?-tubulin and actin genes made it possible to identify 52 isolates as Pa. chlamydospora, 33 as Pm. aleophilum, and one as Pm. australiense. The Pm. aleophilum isolates were divided into three groups by their growth pattern and their colony shape at 37°C: a) white colonies with yellow or brown reverse; b) brown colonies with clear margin and a dark center on the reverse; c) brown colonies with dark-brown diffusible pigments and brown reverse. Further studies are required to explain these differences. Primers Pac1f/Pac2r specific for Phaeoacremonium spp., Pal1N/Pal2 specific for Pm. aleophilum, F2bt/R1bt specific for Pm. aleophilum and ten other Phaeoacremonium species, and Pch1/Pch2 and Pmo1f/Pmo2r, both specific for Pa. chlamydospora were evaluated on the DNA of target fungi and some of other fungi frequently isolated from diseased vine tissues. F2bt/R1bt and Pmo1f/ Pmo2r were selected, and were used in a nested PCR to detect Phaeoacremonium spp. and Pa. chlamydospora in asymptomatic canes of nursery mother grapevines. Nine out of ten sampled grapevines tested positive for one
of the fungi. Molecular diagnosis is potentially a useful method to assess the health of mother grapevines. MenosSummary
Symptoms corresponding to esca and Petri diseases have been described in Uruguay as being associated with Phaeomoniella chlamydospora and Phaeoacremonium spp. Isolates of Phaeoacremonium spp. recovered from diseased grapevines were characterized and identified. Additionally, specific primers developed for Pa. chlamydospora and Phaeoacremonium spp. were evaluated for direct detection of these fungi in asymptomatic grapevine tissues. The bark was removed, and the trunk underneath was surface-disinfected.
Chips from symptomatic grapevines were plated on potato dextrose agar and incubated at 25°C. Isolates were identified morphologically and phylogenetically. Sequences of ITS rDNA, ?-tubulin and actin genes made it possible to identify 52 isolates as Pa. chlamydospora, 33 as Pm. aleophilum, and one as Pm. australiense. The Pm. aleophilum isolates were divided into three groups by their growth pattern and their colony shape at 37°C: a) white colonies with yellow or brown reverse; b) brown colonies with clear margin and a dark center on the reverse; c) brown colonies with dark-brown diffusible pigments and brown reverse. Further studies are required to explain these differences. Primers Pac1f/Pac2r specific for Phaeoacremonium spp., Pal1N/Pal2 specific for Pm. aleophilum, F2bt/R1bt specific for Pm. aleophilum and ten other Phaeoacremonium species, and Pch1/Pch2 and Pmo1f/Pmo2r, both specific for Pa. chlamydospora were evaluated on the DNA of target fungi and some of other ... Presentar Todo |
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Palabras claves : |
ASYMPTOMATIC CANES; ESCA; MONITORING; PETRI DISEASE; SPECIFIC PRIMERS. |
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Thesagro : |
ENFERMEDADES DE LA VID; ENFERMEDADES DE LAS PLANTAS; URUGUAY. |
|
Asunto categoría : |
H20 Enfermedades de las plantas |
|
Marc : |
LEADER 02850naa a2200289 a 4500
001 1050566
005 2019-10-11
008 2011 bl uuuu u00u1 u #d
022 $a1593-2095
024 7 $a10.14601/Phytopathol_Mediterr-8682$2DOI
100 1 $aABREO, E.
245 $aPhaeomoniella chlamydospora and Phaeoacremonium ssp. in gravevines from Uruguay.$h[electronic resource]
260 $c2011
500 $aArticle history: accepted for publication: February 15, 2010; published 2011. http://dx.doi.org/10.14601/Phytopathol_Mediterr-8682
520 $aSummary Symptoms corresponding to esca and Petri diseases have been described in Uruguay as being associated with Phaeomoniella chlamydospora and Phaeoacremonium spp. Isolates of Phaeoacremonium spp. recovered from diseased grapevines were characterized and identified. Additionally, specific primers developed for Pa. chlamydospora and Phaeoacremonium spp. were evaluated for direct detection of these fungi in asymptomatic grapevine tissues. The bark was removed, and the trunk underneath was surface-disinfected. Chips from symptomatic grapevines were plated on potato dextrose agar and incubated at 25°C. Isolates were identified morphologically and phylogenetically. Sequences of ITS rDNA, ?-tubulin and actin genes made it possible to identify 52 isolates as Pa. chlamydospora, 33 as Pm. aleophilum, and one as Pm. australiense. The Pm. aleophilum isolates were divided into three groups by their growth pattern and their colony shape at 37°C: a) white colonies with yellow or brown reverse; b) brown colonies with clear margin and a dark center on the reverse; c) brown colonies with dark-brown diffusible pigments and brown reverse. Further studies are required to explain these differences. Primers Pac1f/Pac2r specific for Phaeoacremonium spp., Pal1N/Pal2 specific for Pm. aleophilum, F2bt/R1bt specific for Pm. aleophilum and ten other Phaeoacremonium species, and Pch1/Pch2 and Pmo1f/Pmo2r, both specific for Pa. chlamydospora were evaluated on the DNA of target fungi and some of other fungi frequently isolated from diseased vine tissues. F2bt/R1bt and Pmo1f/ Pmo2r were selected, and were used in a nested PCR to detect Phaeoacremonium spp. and Pa. chlamydospora in asymptomatic canes of nursery mother grapevines. Nine out of ten sampled grapevines tested positive for one of the fungi. Molecular diagnosis is potentially a useful method to assess the health of mother grapevines.
650 $aENFERMEDADES DE LA VID
650 $aENFERMEDADES DE LAS PLANTAS
650 $aURUGUAY
653 $aASYMPTOMATIC CANES
653 $aESCA
653 $aMONITORING
653 $aPETRI DISEASE
653 $aSPECIFIC PRIMERS
700 1 $aMARTINEZ, S.
700 1 $aBETTUCCI, L.
700 1 $aLUPO, S.
773 $tPhytopathologia Mediterranea. 2011.$gv.50 (Supplement), p.77-85.
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