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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
Fecha : |
12/07/2022 |
Actualizado : |
12/07/2022 |
Tipo de producción científica : |
Capítulo en Libro Técnico-Científico |
Autor : |
KALDS, P.; CRISPO, M.; TESSON, L.; ANEGÓN, I.; CHEN KEY, Y.; WANG, X.; MENCHACA, A. |
Afiliación : |
PETER KALDS, Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, China.; MARTINA CRISPO, Unidad de Biotecnología en Animales de Laboratorio (UBAL), Institut Pasteur de Montevideo, Montevideo, Uruguay.; LAURENT TESSON, INSERM, Centre de Recherche en Transplantation et Immunologie, UMR 1064, Transgenesis Rat ImmunoPhenomic Facility (TRIP), Nantes, France.; IGNACIO ANEGÓN, INSERM, Centre de Recherche en Transplantation et Immunologie, UMR 1064, Nantes, France.; YULIN CHEN KEY, Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, China.; XIAOLONG WANG, Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, China.; JOSE ALEJO MENCHACA BARBEITO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay./Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay. |
Título : |
Generation of Double-Muscled Sheep and Goats by CRISPR /Cas9-Mediated Knockout of the Myostatin Gene. |
Complemento del título : |
Chapter 16. |
Fecha de publicación : |
2022 |
Fuente / Imprenta : |
Methods in Molecular Biology, 2022, Volume 2495, Pages 295-323. Doi: https://doi.org/10.1007/978-1-0716-2301-5_16 |
ISBN : |
Online: 978-1-0716-2301-5 |
DOI : |
10.1007/978-1-0716-2301-5_16 |
Idioma : |
Inglés |
Contenido : |
Abstract:
The myostatin (MSTN) gene has shown to play a critical role in the regulation of skeletal muscle mass, and the translational inhibition of this gene has shown increased muscle mass, generating what is known as ?double-muscling phenotype.? Disruption of the MSTN gene expression using the CRISPR/Cas9 genome-editing system has shown improved muscle development and growth rates in livestock species, including sheep and goats. Here, we describe procedures for the generation of MSTN knockout sheep and goats using the microinjection approach of the CRISPR/Cas9 system, including the selection of targeting sgRNAs, the construction of CRISPR/Cas9 targeting vector, the in vitro examination of system efficiency, the in vivo targeting to generate MSTN knockout founders, the genomic and phenotypic characterization of the generated offspring, and the assessment of off-target effects in gene-edited founders through targeted validation of predicted off-target sites, as well as genome-wide off-target analysis by whole-genome sequencing. Editing the MSTN gene using the CRISPR/Cas9 system might be a rapid and promising alternative to promote meat production in livestock. |
Palabras claves : |
CRISPR/Cas9; Genome editing; Goats; Knockout; Meat production; Microinjection; MSTN; Sheep; Small ruminants. |
Thesagro : |
CABRAS; OVEJA; PRODUCCION DE CARNE. |
Asunto categoría : |
-- |
Marc : |
LEADER 02176naa a2200349 a 4500 001 1063414 005 2022-07-12 008 2022 bl uuuu u00u1 u #d 024 7 $a10.1007/978-1-0716-2301-5_16$2DOI 100 1 $aKALDS, P. 245 $aGeneration of Double-Muscled Sheep and Goats by CRISPR /Cas9-Mediated Knockout of the Myostatin Gene.$h[electronic resource] 260 $c2022 520 $aAbstract: The myostatin (MSTN) gene has shown to play a critical role in the regulation of skeletal muscle mass, and the translational inhibition of this gene has shown increased muscle mass, generating what is known as ?double-muscling phenotype.? Disruption of the MSTN gene expression using the CRISPR/Cas9 genome-editing system has shown improved muscle development and growth rates in livestock species, including sheep and goats. Here, we describe procedures for the generation of MSTN knockout sheep and goats using the microinjection approach of the CRISPR/Cas9 system, including the selection of targeting sgRNAs, the construction of CRISPR/Cas9 targeting vector, the in vitro examination of system efficiency, the in vivo targeting to generate MSTN knockout founders, the genomic and phenotypic characterization of the generated offspring, and the assessment of off-target effects in gene-edited founders through targeted validation of predicted off-target sites, as well as genome-wide off-target analysis by whole-genome sequencing. Editing the MSTN gene using the CRISPR/Cas9 system might be a rapid and promising alternative to promote meat production in livestock. 650 $aCABRAS 650 $aOVEJA 650 $aPRODUCCION DE CARNE 653 $aCRISPR/Cas9 653 $aGenome editing 653 $aGoats 653 $aKnockout 653 $aMeat production 653 $aMicroinjection 653 $aMSTN 653 $aSheep 653 $aSmall ruminants 700 1 $aCRISPO, M. 700 1 $aTESSON, L. 700 1 $aANEGÓN, I. 700 1 $aCHEN KEY, Y. 700 1 $aWANG, X. 700 1 $aMENCHACA, A. 773 $tMethods in Molecular Biology, 2022, Volume 2495, Pages 295-323. Doi: https://doi.org/10.1007/978-1-0716-2301-5_16
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INIA La Estanzuela (LE) |
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Biblioteca (s) : |
INIA Las Brujas; INIA Tacuarembó. |
Fecha actual : |
21/02/2014 |
Actualizado : |
21/10/2019 |
Autor : |
Monteverde, E.Speranza, P.Galván, G. |
Título : |
Análisis preliminar de la diversidad genética en una colección de variedades criollas de cebolla del Uruguay |
Fecha de publicación : |
2009 |
Fuente / Imprenta : |
Las Brujas, Canelones (Uruguay): INIA, 2009. |
Páginas : |
p. 21-26 |
Serie : |
(INIA Serie Actividades de Difusión ; 564) |
Idioma : |
Español |
Notas : |
INIA Las Brujas |
Thesagro : |
CEBOLLA; URUGUAY. |
Asunto categoría : |
-- F01 Cultivo |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/549/1/18429010409154339.pdf
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Marc : |
LEADER 00551naa a2200169 a 4500 001 1008444 005 2019-10-21 008 2009 bl uuuu u00u1 u #d 100 1 $aMONTEVERDE, E.SPERANZA, P.GALVÁN, G. 245 $aAnálisis preliminar de la diversidad genética en una colección de variedades criollas de cebolla del Uruguay 260 $c2009 300 $ap. 21-26 490 $a(INIA Serie Actividades de Difusión ; 564) 500 $aINIA Las Brujas 650 $aCEBOLLA 650 $aURUGUAY 773 $tLas Brujas, Canelones (Uruguay): INIA, 2009.
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