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Registro completo
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Biblioteca (s) : |
INIA Treinta y Tres. |
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Fecha : |
05/04/2022 |
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Actualizado : |
01/09/2022 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
SCHVARTZMAN, C.; FRESIA, P.; MURCHIO, S.; MUJICA, V.; DALLA RIZZA, M. |
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Afiliación : |
CLAUDIA SCHVARTZMAN DISEGNI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; PABLO FRESIA, Unidad Mixta UMPI, Institut Pasteur Montevideo + INIA, Montevideo, Uruguay; MARIA SARA MURCHIO VIGNOLO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARIA VALENTINA MUJICA TELIZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARCO DALLA RIZZA VILARO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
RNAi in Piezodorus guildinii (Hemiptera: Pentatomidae): transcriptome assembly for the development of pest control strategies. |
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Fecha de publicación : |
2022 |
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Fuente / Imprenta : |
Frontiers in Plant Science, 01 April 2022, volume13, article 804839. OPEN ACCESS. Doi: https://doi.org/10.3389/fpls.2022.804839 |
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Páginas : |
13 p. |
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DOI : |
10.3389/fpls.2022.804839 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: 29 October 2021; Accepted: 28 February 2022; Published: 01 April 2022.
Correspondence: Marco Dalla-Rizza, mdallarizza@inia.org.uy
Al abrir el registro se visualiza el enlace al material suplementario. |
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Contenido : |
Red-banded stink bug Piezodorus guildinii (P. guildinii) has been described as the most damaging stink bug regarding soybean crops, leading to seed injury, low germination percentages, and foliar retention, at low population densities. In recent years, RNA interference (RNAi), a conserved eukaryote silencing mechanism has been explored to develop species-selective pesticides. In this work, we evaluated RNAi in P. guildinii to develop new pest-control strategies. For this, we assembled and annotated a P. guildinii transcriptome from a pool of all developmental stages. Analysis of this transcriptome led to the identification of 56 genes related to the silencing process encompassing siRNA, miRNA, and piRNA pathways. To evaluate the functionality of RNAi machinery, P. guildinii adults were injected with 28 ng/mg of body weight of double stranded RNA (dsRNA) targeting vATPase A. A mortality of 35 and 51.6% was observed after 7 and 14 days, respectively, and a downregulation of vATPase A gene of 84% 72 h post-injection. In addition, Dicer-2 and Argonaute-2 genes, core RNAi proteins, were upregulated 1.8-fold 48 h after injection. These findings showed for the first time that RNAi is functional in P. guildinii and the silencing of essential genes has a significant effect in adult viability. Taken together, the work reported here shows that RNAi could be an interesting approach for the development of red-banded stink bug control strategies. |
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Palabras claves : |
DSRNA,; PEST CONTROL; RNA-SEQ; RNAI; STINK BUG; VATPASE A,. |
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Asunto categoría : |
H10 Plagas de las plantas |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/16649/1/Frontiers-Plant-Science-2022-Schvartzman.pdf
http://www.ainfo.inia.uy/digital/bitstream/item/16650/1/Frontiers-Plant-Science-2022-Schvartzman-1.PDF
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Marc : |
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024 7 $a10.3389/fpls.2022.804839$2DOI
100 1 $aSCHVARTZMAN, C.
245 $aRNAi in Piezodorus guildinii (Hemiptera$bPentatomidae): transcriptome assembly for the development of pest control strategies.$h[electronic resource]
260 $c2022
300 $a13 p.
500 $aArticle history: Received: 29 October 2021; Accepted: 28 February 2022; Published: 01 April 2022. Correspondence: Marco Dalla-Rizza, mdallarizza@inia.org.uy Al abrir el registro se visualiza el enlace al material suplementario.
520 $aRed-banded stink bug Piezodorus guildinii (P. guildinii) has been described as the most damaging stink bug regarding soybean crops, leading to seed injury, low germination percentages, and foliar retention, at low population densities. In recent years, RNA interference (RNAi), a conserved eukaryote silencing mechanism has been explored to develop species-selective pesticides. In this work, we evaluated RNAi in P. guildinii to develop new pest-control strategies. For this, we assembled and annotated a P. guildinii transcriptome from a pool of all developmental stages. Analysis of this transcriptome led to the identification of 56 genes related to the silencing process encompassing siRNA, miRNA, and piRNA pathways. To evaluate the functionality of RNAi machinery, P. guildinii adults were injected with 28 ng/mg of body weight of double stranded RNA (dsRNA) targeting vATPase A. A mortality of 35 and 51.6% was observed after 7 and 14 days, respectively, and a downregulation of vATPase A gene of 84% 72 h post-injection. In addition, Dicer-2 and Argonaute-2 genes, core RNAi proteins, were upregulated 1.8-fold 48 h after injection. These findings showed for the first time that RNAi is functional in P. guildinii and the silencing of essential genes has a significant effect in adult viability. Taken together, the work reported here shows that RNAi could be an interesting approach for the development of red-banded stink bug control strategies.
653 $aDSRNA,
653 $aPEST CONTROL
653 $aRNA-SEQ
653 $aRNAI
653 $aSTINK BUG
653 $aVATPASE A,
700 1 $aFRESIA, P.
700 1 $aMURCHIO, S.
700 1 $aMUJICA, V.
700 1 $aDALLA RIZZA, M.
773 $tFrontiers in Plant Science, 01 April 2022, volume13, article 804839. OPEN ACCESS. Doi: https://doi.org/10.3389/fpls.2022.804839
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INIA Treinta y Tres (TT) |
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| Registros recuperados : 6 | |
| 1. |  | KHU, D.; REYNO, R.; BRUMMER, E.; MONTEROS, M. Screening methods for aluminum tolerance in Alfalfa. Crop Science, 2012, v. 52, p. 161 - 167. DOI: https://doi.org/10.2135/cropsci2011.05.0256 52 Article history: Published online 4 Oct. 2011. Acknowledgments:This work was funded by the Samuel Roberts Noble Foundation. We thank Christy Motes, Kazuyo Ueda, and Will Chaney
for their assistance with the whole plant assay in media and...| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA Tacuarembó. |
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| 3. | | REYNO, R.; DONG-MAN, K.; MONTEROS, M.J.; BOUTON, J.H.; PARROTT, W.; BRUMMER, E.C. Evaluation of two transgenes for aluminum tolerance in alfalfa. Crop Science, 2013, v. 53, no. 4, p. 1581-1588. DOI: https://doi.org/10.2135/cropsci2012.12.0676 History article: Received 3 Dec. 2012. Acknowledgments The authors would like to thank Donald Wood, Jonathan Markham, and Wesley Dean for their support with the greenhouse screening, Dr. Nathan Hancock for helping with the Southern blot...| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA Tacuarembó. |
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| 4. | | KHU, D.; REYNO, R.; HAN, Y.; ZHAO, P.; BOUTON, J.; BRUMMER, E.; MONTEROS, M. Identification of Aluminum Tolerance Quantitative Trait Loci in Tetraploid Alfalfa. Crop Science, 2013, v. 53, no 1, p. 148 - 163. DO: https://doi.org/10.2135/cropsci2012.03.0181 History article: Received 15 Mar. 2012. Acknowledgments: We would like to thank Kazuyo Ueda, Christy Motes, Will Chaney, Zachary Ishikawa, and Craig Schluttenhofer for their
assistance with tissue culture and Xuehui Li for developing the...| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA Tacuarembó. |
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| 6. | | KHU, D.; REYNO, R.; BRUMMER, E.; BOUTON, J.; HAN, Y.; MONTEROS, M. QTL mapping of aluminum tolerance in tetraploid alfalfa In: Huyghe, C., ed. Sustainable use of Genetic Diversity in Forage and Turf Breeding. Lusignan, (FR): Springer, 2010, cap. 64. 437-442| Tipo: Capítulo en Libro Técnico-Científico |
| Biblioteca(s): INIA Tacuarembó. |
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| Registros recuperados : 6 | |
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