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| Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
Registro completo
|
Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
17/05/2022 |
Actualizado : |
02/12/2022 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
PASSOS, J. R. S.; GUERREIRO, D. D.; OTÁVIO, K. S.; SANTOS-NETO, P. C. DOS; SOUZA-NEVES, M.; CUADRO, F.; NUÑEZ-OLIVERA, R.; CRISPO, M.; BEZERRA, M. J. B.; SILVA, R. F.; LIMA, L. F.; FIGUEIREDO, J. R.; BUSTAMANTE-FILHO, I. C.; MENCHACA, A.; MOURA, A. A. |
Afiliación : |
JOSÉ RENATO S. PASSOS, Laboratório de Fisiologia e Ciências Ômicas, Departamento de Zootecnia, Universidade Federal do Ceará, Fortaleza, Brazil; DENISE D. GUERREIRO, Laboratório de Fisiologia e Ciências Ômicas, Departamento de Zootecnia, Universidade Federal do Ceará, Fortaleza, Brazil; KAMILA S. OTÁVIO, Laboratório de Fisiologia e Ciências Ômicas, Departamento de Zootecnia, Universidade Federal do Ceará, Fortaleza, Brazil; P. C. DOS SANTOS-NETO, Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay; MARCELA SOUZA-NEVES, Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay; FEDERICO CUADRO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay; RICHARD NUÑEZ-OLIVERA, Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay; MARTINA CRISPO, Unidad de Biotecnología en Animales de Laboratorio, Institut Pasteur de Montevideo, Montevideo, Uruguay; MARIA JÚLIA B. BEZERRA, Laboratório de Fisiologia e Ciências Ômicas, Departamento de Zootecnia, Universidade Federal do Ceará, Fortaleza, Brazil; RENATO F. SILVA, Laboratório de Manipulação de Oócitos e Folículos Ovarianos Pré-antrais - LAMOFOPA - Faculdade de Veterinária, Universidade Estadual do Ceará, Fortaleza, Brazil; LARITZA F. LIMA, Laboratório de Manipulação de Oócitos e Folículos Ovarianos Pré-antrais - LAMOFOPA - Faculdade de Veterinária, Universidade Estadual do Ceará, Fortaleza, Brazil; JOSÉ RICARDO FIGUEIREDO, Laboratório de Manipulação de Oócitos e Folículos Ovarianos Pré-antrais - LAMOFOPA - Faculdade de Veterinária, Universidade Estadual do Ceará, Fortaleza, Brazil; IVAN C. BUSTAMANTE-FILHO, aboratório de Biotecnologia da Reprodução Animal, Programa de Pós-graduação em Biotecnologia, Universidade do Vale do Taquari, Lajeado, Brazil; JOSE ALEJO MENCHACA BARBEITO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay; ARLINDO A. MOURA, Laboratório de Fisiologia e Ciências Ômicas, Departamento de Zootecnia, Universidade Federal do Ceará, Fortaleza, Brazil. |
Título : |
Global proteomic analysis of preimplantational ovine embryos produced in vitro. |
Fecha de publicación : |
2022 |
Fuente / Imprenta : |
Reproduction in Domestic Animals, 2022, Volume 57, Issue 7; pages 784-797. doi: https://doi.org/10.1111/rda.14122 |
ISSN : |
0936-6768 |
DOI : |
10.1111/rda.14122 |
Idioma : |
Inglés |
Notas : |
Article history: Received 15 February 2022; Accepted 1 April 2022. -- Funding text - The experiments presently described were conducted at the facilities of the (Fundacion IRAUy, Montevideo, Uruguay) and at the (UBAL) of the , Uruguay. Specially, the authors thank Dr. Rosario Durán and Dr. Alejandro Leyva for kindly assisting us in the proteomic experiment. Finnacial support was provided by Fundacion IRAUy; PRONEX 02/2015 (Programa de Apoio a Núcleos de Excelência Pronex/Funcap/CNPq); the Brazilian Research Council?CNPq (grants # 313160/2017‐1 and 438773/2018‐7); Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Brazil. Instituto de Reproducción Animal Uruguay Unidad de Biotecnología en Animales de Laboratorio Institut Pasteur de Montevideo. -- Corresponding author: A. Moura, A.; Laboratório de Fisiologia e Ciências Ômicas, Departamento de Zootecnia, Universidade Federal do Ceará, Fortaleza, Brazil; email:arlindo.moura@gmail.com -- Menchaca, A.; Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay; mail:menchaca.alejo@gmail.com |
Contenido : |
ABSTRACT. -The present study was conducted to characterize the major proteome of preimplantation (D6) ovine embryos produced in vitro. COCs were aspirated from antral follicles (2–6 mm), matured and fertilized in vitro and cultured until day six. Proteins were ex- tracted separately from three pools of 45 embryos and separately run in SDS-PAGE. Proteins from each pool were individually subjected to in-gel digestion followed by LC-MS/MS. Three ‘raw files’ and protein lists were produced by Pattern Lab software, but only proteins present in all three lists were used for the bioinformatics analyses. There were 2,262 proteins identified in the 6-day-old ovine
embryos, including al- bumin, zona pellucida glycoprotein 2, 3 and 4, peptidyl arginine deiminase 6, actin cytoplasmic 1, gamma-actin 1, pyruvate kinase, heat shock protein 90 and protein disulfide isomerase, among others. Major biological processes linked to the sheep embryo proteome were translation, protein transport and protein stabilization, and molecular functions, defined as ATP binding, oxygen carrier activity and oxygen bind- ing. There were 42 enriched functional clusters
according to the 2,147 genes (UniProt database). Ten selected clusters with potential association with embryo development included translation, structural constituent of ribosomes, ribosomes, nucleosomes, structural constituent of the cytoskeleton, microtubule-based process, translation initiation factor activity, regulation of translational initiation, cell body and nucleotide biosynthetic process. The most representative KEEG pathways were ribosome, oxida- tive phosphorylation, glutathione metabolism, gap junction, mineral absorption, DNA replication and cGMP-PKG signalling pathway. Analyses of functional clusters clearly showed differences associated
with the proteome of preimplantation (D6) sheep em- bryos generated after in vitro fertilization in comparison with in vivo counterparts (Sanchez et al., 2021; https://doi.org/10.1111/rda.13897), confirming that the quality of in vitro derived blastocysts are unlike those produced in vivo. The present study portrays the first comprehensive overview of the proteome of preimplantational ovine embryos grown in vitro.
© 2022 Wiley-VCH GmbH. MenosABSTRACT. -The present study was conducted to characterize the major proteome of preimplantation (D6) ovine embryos produced in vitro. COCs were aspirated from antral follicles (2–6 mm), matured and fertilized in vitro and cultured until day six. Proteins were ex- tracted separately from three pools of 45 embryos and separately run in SDS-PAGE. Proteins from each pool were individually subjected to in-gel digestion followed by LC-MS/MS. Three ‘raw files’ and protein lists were produced by Pattern Lab software, but only proteins present in all three lists were used for the bioinformatics analyses. There were 2,262 proteins identified in the 6-day-old ovine
embryos, including al- bumin, zona pellucida glycoprotein 2, 3 and 4, peptidyl arginine deiminase 6, actin cytoplasmic 1, gamma-actin 1, pyruvate kinase, heat shock protein 90 and protein disulfide isomerase, among others. Major biological processes linked to the sheep embryo proteome were translation, protein transport and protein stabilization, and molecular functions, defined as ATP binding, oxygen carrier activity and oxygen bind- ing. There were 42 enriched functional clusters
according to the 2,147 genes (UniProt database). Ten selected clusters with potential association with embryo development included translation, structural constituent of ribosomes, ribosomes, nucleosomes, structural constituent of the cytoskeleton, microtubule-based process, translation initiation factor activity, regulation of translational i... Presentar Todo |
Palabras claves : |
Embryo development; In vitro fertilization; Mass spectrometry; Oocyte; Ovine; PLATAFORMA SALUD ANIMAL; Proteins. |
Asunto categoría : |
L10 Genética y mejoramiento animal |
Marc : |
LEADER 04571naa a2200409 a 4500 001 1063149 005 2022-12-02 008 2022 bl uuuu u00u1 u #d 022 $a0936-6768 024 7 $a10.1111/rda.14122$2DOI 100 1 $aPASSOS, J. R. S. 245 $aGlobal proteomic analysis of preimplantational ovine embryos produced in vitro.$h[electronic resource] 260 $c2022 500 $aArticle history: Received 15 February 2022; Accepted 1 April 2022. -- Funding text - The experiments presently described were conducted at the facilities of the (Fundacion IRAUy, Montevideo, Uruguay) and at the (UBAL) of the , Uruguay. Specially, the authors thank Dr. Rosario Durán and Dr. Alejandro Leyva for kindly assisting us in the proteomic experiment. Finnacial support was provided by Fundacion IRAUy; PRONEX 02/2015 (Programa de Apoio a Núcleos de Excelência Pronex/Funcap/CNPq); the Brazilian Research Council?CNPq (grants # 313160/2017‐1 and 438773/2018‐7); Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Brazil. Instituto de Reproducción Animal Uruguay Unidad de Biotecnología en Animales de Laboratorio Institut Pasteur de Montevideo. -- Corresponding author: A. Moura, A.; Laboratório de Fisiologia e Ciências Ômicas, Departamento de Zootecnia, Universidade Federal do Ceará, Fortaleza, Brazil; email:arlindo.moura@gmail.com -- Menchaca, A.; Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay; mail:menchaca.alejo@gmail.com 520 $aABSTRACT. -The present study was conducted to characterize the major proteome of preimplantation (D6) ovine embryos produced in vitro. COCs were aspirated from antral follicles (2–6 mm), matured and fertilized in vitro and cultured until day six. Proteins were ex- tracted separately from three pools of 45 embryos and separately run in SDS-PAGE. Proteins from each pool were individually subjected to in-gel digestion followed by LC-MS/MS. Three ‘raw files’ and protein lists were produced by Pattern Lab software, but only proteins present in all three lists were used for the bioinformatics analyses. There were 2,262 proteins identified in the 6-day-old ovine embryos, including al- bumin, zona pellucida glycoprotein 2, 3 and 4, peptidyl arginine deiminase 6, actin cytoplasmic 1, gamma-actin 1, pyruvate kinase, heat shock protein 90 and protein disulfide isomerase, among others. Major biological processes linked to the sheep embryo proteome were translation, protein transport and protein stabilization, and molecular functions, defined as ATP binding, oxygen carrier activity and oxygen bind- ing. There were 42 enriched functional clusters according to the 2,147 genes (UniProt database). Ten selected clusters with potential association with embryo development included translation, structural constituent of ribosomes, ribosomes, nucleosomes, structural constituent of the cytoskeleton, microtubule-based process, translation initiation factor activity, regulation of translational initiation, cell body and nucleotide biosynthetic process. The most representative KEEG pathways were ribosome, oxida- tive phosphorylation, glutathione metabolism, gap junction, mineral absorption, DNA replication and cGMP-PKG signalling pathway. Analyses of functional clusters clearly showed differences associated with the proteome of preimplantation (D6) sheep em- bryos generated after in vitro fertilization in comparison with in vivo counterparts (Sanchez et al., 2021; https://doi.org/10.1111/rda.13897), confirming that the quality of in vitro derived blastocysts are unlike those produced in vivo. The present study portrays the first comprehensive overview of the proteome of preimplantational ovine embryos grown in vitro. © 2022 Wiley-VCH GmbH. 653 $aEmbryo development 653 $aIn vitro fertilization 653 $aMass spectrometry 653 $aOocyte 653 $aOvine 653 $aPLATAFORMA SALUD ANIMAL 653 $aProteins 700 1 $aGUERREIRO, D. D. 700 1 $aOTÁVIO, K. S. 700 1 $aSANTOS-NETO, P. C. DOS 700 1 $aSOUZA-NEVES, M. 700 1 $aCUADRO, F. 700 1 $aNUÑEZ-OLIVERA, R. 700 1 $aCRISPO, M. 700 1 $aBEZERRA, M. J. B. 700 1 $aSILVA, R. F. 700 1 $aLIMA, L. F. 700 1 $aFIGUEIREDO, J. R. 700 1 $aBUSTAMANTE-FILHO, I. C. 700 1 $aMENCHACA, A. 700 1 $aMOURA, A. A. 773 $tReproduction in Domestic Animals, 2022, Volume 57, Issue 7; pages 784-797. doi: https://doi.org/10.1111/rda.14122
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| Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
Fecha actual : |
09/10/2019 |
Actualizado : |
09/10/2019 |
Tipo de producción científica : |
Poster |
Autor : |
MICHELOUD, J.F.; COLQUE CARO, L.A.; SUAREZ, V.H.; RIET-CORREA, F. |
Afiliación : |
JUAN FRANCISCO MICHELOUD, AISA-IIACS Salta/INTA. RN 68, km 72- Salta, Argentina.; LUIS ADRIAN COLQUE CARO, Facultad de Ciencias Agrarias y Veterinarias-UCASAL.; VICTOR HUMBERTO SUAREZ, AISA-IIACS Salta/INTA. RN 68, km 72- Salta, Argentina.; FRANKLIN RIET-CORREA AMARAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
Uso de tiras reactivas de orina para estimar la prevalencia en rodeos afectados por hematuria enzootica bovina. |
Fecha de publicación : |
2016 |
Fuente / Imprenta : |
In:Reunión Científica Técnica de la Asociación Argentina de Veterinarios de Laboratorios de diagnóstico (AAVLD), 21, San Salvador de Jujuy, Argentina, 2016. |
Idioma : |
Español |
Contenido : |
INTRODUCCIÓN: La hematuria enzoótica Bovina (HEB) es una enfermedad mundialmente distribuida causada por la ingestión prolongada de ptaquilosidos (PT) presentes en algunos helechos (P. arachnoideum)1. Se caracteriza por la presencia de lesiones neoplásicas en la vejiga urinaria siendo el signo principal la aparición de sangre en orina. La presencia de hematuria, puede ser usada como estimador de prevalencia en rodeos donde la enfermedad es endémica2. Este trabajo evalúa el uso de las tiras reactivas de orina como método para cuantificar la presencia de hematuria en " rodeos endémicos " y " no endémicos " para HEB en comparación con la evaluación del sedimento urinario. |
Palabras claves : |
HEMATURIA ENZOÓTICA BOVINA (HEB); PLATAFORMA SALUD ANIMAL. |
Asunto categoría : |
L73 Enfermedades de los animales |
Marc : |
LEADER 01373nam a2200169 a 4500 001 1060304 005 2019-10-09 008 2016 bl uuuu u00u1 u #d 100 1 $aMICHELOUD, J.F. 245 $aUso de tiras reactivas de orina para estimar la prevalencia en rodeos afectados por hematuria enzootica bovina.$h[electronic resource] 260 $aIn:Reunión Científica Técnica de la Asociación Argentina de Veterinarios de Laboratorios de diagnóstico (AAVLD), 21, San Salvador de Jujuy, Argentina$c2016 520 $aINTRODUCCIÓN: La hematuria enzoótica Bovina (HEB) es una enfermedad mundialmente distribuida causada por la ingestión prolongada de ptaquilosidos (PT) presentes en algunos helechos (P. arachnoideum)1. Se caracteriza por la presencia de lesiones neoplásicas en la vejiga urinaria siendo el signo principal la aparición de sangre en orina. La presencia de hematuria, puede ser usada como estimador de prevalencia en rodeos donde la enfermedad es endémica2. Este trabajo evalúa el uso de las tiras reactivas de orina como método para cuantificar la presencia de hematuria en " rodeos endémicos " y " no endémicos " para HEB en comparación con la evaluación del sedimento urinario. 653 $aHEMATURIA ENZOÓTICA BOVINA (HEB) 653 $aPLATAFORMA SALUD ANIMAL 700 1 $aCOLQUE CARO, L.A. 700 1 $aSUAREZ, V.H. 700 1 $aRIET-CORREA, F.
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