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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
Fecha : |
21/02/2014 |
Actualizado : |
22/02/2014 |
Autor : |
Castro, A. ; Hoffman, E. ; Ernst, O. |
Título : |
Evolución del rendimiento en grano y sus componentes : situación actual y perspectivas del mejoramiento genetico |
Fecha de publicación : |
1993 |
Fuente / Imprenta : |
ln: Reunión Nacional de Investigadores de Cebada, 4 : 1993 jun 3-4 : Palmar ; Mesa Nacional de Entidades de Cebada Cervecera [Memorias]. Montevideo (Uruguay): INIA; LATU; SALUS, 1993. |
Páginas : |
p60-70 |
ISBN : |
9974-0-0034-3 |
Idioma : |
Español |
Notas : |
Edición preliminar |
Thesagro : |
CEBADA; CEBADA CERVECERA; FACTORES DE RENDIMIENTO; FITOMEJORAMIENTO; GRANOS; RENDIMIENTO; URUGUAY. |
Asunto categoría : |
-- |
Marc : |
LEADER 00849naa a2200253 a 4500 001 1044635 005 2014-02-22 008 1993 bl uuuu u00u1 u #d 020 $a 9974-0-0034-3 100 1 $aCASTRO, A. 245 $aEvolución del rendimiento en grano y sus componentes$bsituación actual y perspectivas del mejoramiento genetico 260 $c1993 300 $ap60-70 500 $aEdición preliminar 650 $aCEBADA 650 $aCEBADA CERVECERA 650 $aFACTORES DE RENDIMIENTO 650 $aFITOMEJORAMIENTO 650 $aGRANOS 650 $aRENDIMIENTO 650 $aURUGUAY 700 1 $aHOFFMAN, E. 700 1 $aERNST, O. 773 $tln: Reunión Nacional de Investigadores de Cebada, 4 : 1993 jun 3-4 : Palmar ; Mesa Nacional de Entidades de Cebada Cervecera [Memorias]. Montevideo (Uruguay): INIA; LATU; SALUS, 1993.
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INIA La Estanzuela (LE) |
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| Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
Fecha actual : |
12/07/2022 |
Actualizado : |
12/07/2022 |
Tipo de producción científica : |
Capítulo en Libro Técnico-Científico |
Autor : |
KALDS, P.; CRISPO, M.; TESSON, L.; ANEGÓN, I.; CHEN KEY, Y.; WANG, X.; MENCHACA, A. |
Afiliación : |
PETER KALDS, Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, China.; MARTINA CRISPO, Unidad de Biotecnología en Animales de Laboratorio (UBAL), Institut Pasteur de Montevideo, Montevideo, Uruguay.; LAURENT TESSON, INSERM, Centre de Recherche en Transplantation et Immunologie, UMR 1064, Transgenesis Rat ImmunoPhenomic Facility (TRIP), Nantes, France.; IGNACIO ANEGÓN, INSERM, Centre de Recherche en Transplantation et Immunologie, UMR 1064, Nantes, France.; YULIN CHEN KEY, Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, China.; XIAOLONG WANG, Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, China.; JOSE ALEJO MENCHACA BARBEITO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay./Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay. |
Título : |
Generation of Double-Muscled Sheep and Goats by CRISPR /Cas9-Mediated Knockout of the Myostatin Gene. |
Complemento del título : |
Chapter 16. |
Fecha de publicación : |
2022 |
Fuente / Imprenta : |
Methods in Molecular Biology, 2022, Volume 2495, Pages 295-323. Doi: https://doi.org/10.1007/978-1-0716-2301-5_16 |
ISBN : |
Online: 978-1-0716-2301-5 |
DOI : |
10.1007/978-1-0716-2301-5_16 |
Idioma : |
Inglés |
Contenido : |
Abstract:
The myostatin (MSTN) gene has shown to play a critical role in the regulation of skeletal muscle mass, and the translational inhibition of this gene has shown increased muscle mass, generating what is known as ?double-muscling phenotype.? Disruption of the MSTN gene expression using the CRISPR/Cas9 genome-editing system has shown improved muscle development and growth rates in livestock species, including sheep and goats. Here, we describe procedures for the generation of MSTN knockout sheep and goats using the microinjection approach of the CRISPR/Cas9 system, including the selection of targeting sgRNAs, the construction of CRISPR/Cas9 targeting vector, the in vitro examination of system efficiency, the in vivo targeting to generate MSTN knockout founders, the genomic and phenotypic characterization of the generated offspring, and the assessment of off-target effects in gene-edited founders through targeted validation of predicted off-target sites, as well as genome-wide off-target analysis by whole-genome sequencing. Editing the MSTN gene using the CRISPR/Cas9 system might be a rapid and promising alternative to promote meat production in livestock. |
Palabras claves : |
CRISPR/Cas9; Genome editing; Goats; Knockout; Meat production; Microinjection; MSTN; Sheep; Small ruminants. |
Thesagro : |
CABRAS; OVEJA; PRODUCCION DE CARNE. |
Asunto categoría : |
-- |
Marc : |
LEADER 02176naa a2200349 a 4500 001 1063414 005 2022-07-12 008 2022 bl uuuu u00u1 u #d 024 7 $a10.1007/978-1-0716-2301-5_16$2DOI 100 1 $aKALDS, P. 245 $aGeneration of Double-Muscled Sheep and Goats by CRISPR /Cas9-Mediated Knockout of the Myostatin Gene.$h[electronic resource] 260 $c2022 520 $aAbstract: The myostatin (MSTN) gene has shown to play a critical role in the regulation of skeletal muscle mass, and the translational inhibition of this gene has shown increased muscle mass, generating what is known as ?double-muscling phenotype.? Disruption of the MSTN gene expression using the CRISPR/Cas9 genome-editing system has shown improved muscle development and growth rates in livestock species, including sheep and goats. Here, we describe procedures for the generation of MSTN knockout sheep and goats using the microinjection approach of the CRISPR/Cas9 system, including the selection of targeting sgRNAs, the construction of CRISPR/Cas9 targeting vector, the in vitro examination of system efficiency, the in vivo targeting to generate MSTN knockout founders, the genomic and phenotypic characterization of the generated offspring, and the assessment of off-target effects in gene-edited founders through targeted validation of predicted off-target sites, as well as genome-wide off-target analysis by whole-genome sequencing. Editing the MSTN gene using the CRISPR/Cas9 system might be a rapid and promising alternative to promote meat production in livestock. 650 $aCABRAS 650 $aOVEJA 650 $aPRODUCCION DE CARNE 653 $aCRISPR/Cas9 653 $aGenome editing 653 $aGoats 653 $aKnockout 653 $aMeat production 653 $aMicroinjection 653 $aMSTN 653 $aSheep 653 $aSmall ruminants 700 1 $aCRISPO, M. 700 1 $aTESSON, L. 700 1 $aANEGÓN, I. 700 1 $aCHEN KEY, Y. 700 1 $aWANG, X. 700 1 $aMENCHACA, A. 773 $tMethods in Molecular Biology, 2022, Volume 2495, Pages 295-323. Doi: https://doi.org/10.1007/978-1-0716-2301-5_16
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