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 | Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha : |
18/12/2020 |
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Actualizado : |
18/12/2020 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
DORSCH, M.; REGIDOR-CERRILLO, J.; ORTEGA-MORA, L.M.; SCIOLI, M.V.; MORRELL, E.L.; CANTÓN, G.J.; MOORE, D.P.; HECKER, Y.P. |
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Afiliación : |
MATÍAS ANDRÉS DORSCH, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay;Faculty of Agrarian Sciences, National University of Mar del Plata (UNMdP), Mar del Plata, Argentina; JAVIER REGIDOR-CERRILLO, Animal Health Department, Faculty of Veterinary Sciences, Complutense University of Madrid, Madrid, Spain.; LUIS M. ORTEGA-MORA, Animal Health Department, Faculty of Veterinary Sciences, Complutense University of Madrid, Madrid, Spain.; MARÍA V. SCIOLI, María V. Scioli National Institute of Agrarian Technology (INTA), Buenos Aires, Argentina.; ELEONORA L. MORRELL, National Institute of Agrarian Technology (INTA), Buenos Aires, Argentina.; GERMÁN J. CANTÓN, National Institute of Agrarian Technology (INTA), Buenos Aires, Argentina.; DADÍN P. MOORE, Faculty of Agrarian Sciences, National University of Mar del Plata (UNMdP), Mar del Plata, Argentina.; YANINA P. HECKER, National Institute of Agrarian Technology (INTA), Buenos Aires, Argentina. |
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Título : |
Microsatellite genotyping reveals extensive genetic diversity in bovine Neospora caninum from the humid Pampa region in Argentina. |
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Fecha de publicación : |
2020 |
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Fuente / Imprenta : |
Parasitology Research, December 2020, Volume 119, Issue 12, Pages 4049-4059. Doi: https://doi-org/10.1007/s00436-020-06922-x |
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DOI : |
10.1007/s00436-020-06922-x |
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Idioma : |
Inglés |
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Notas : |
Article history:Received: 12 August 2020 /Accepted: 5 October 2020 |
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Contenido : |
Abstract: Neospora caninum is an apicomplexan protozoan and a major cause of abortion in cattle worldwide. In the Argentinian Humid Pampa, bovine neosporosis causes severe economic losses. Despite this, information on the genetic structure of N. caninum in this region is limited. Therefore, this study aimed to genetically characterize N. caninum isolates associated with bovine abortion in the Humid Pampa region. For this purpose, spontaneous bovine fetal tissues submitted for diagnosis to the Veterinary Diagnostic Service at INTA Balcarce during 2008?2019 were assessed by PCR, indirect fluorescent antibody test (IFAT), and histologic analysis. PCR-positive samples were tested by multilocus microsatellite genotyping (MLGs) using 9 microsatellite markers. Thirty-one different genotypes were identified from 32 samples with at least seven markers. Argentinian MLGs were grouped into two clonal clusters when analyzed using eBURST network and principal coordinate analysis. No segregation based on the year of collection, animal biotype, or geographic origin was observed. In addition, the presence of linkage disequilibrium supported the clonal propagation of Argentinian MLGs. One Argentinian subpopulation was associated with isolates from Spain, Uruguay, Brazil, and Mexico, and the other one was linked to isolates from Scotland, Spain, and Germany. These findings reveal the presence of two clonal subpopulations of N. caninum in the Humid Pampa. |
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Palabras claves : |
ABORTION; BOVINE; MICROSATELLITE MARKERS; NEOSPORA CANINUM; PLATAFORMA SALUD ANIMAL. |
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Thesagro : |
ARGENTINA. |
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Asunto categoría : |
-- |
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Marc : |
LEADER 02492naa a2200301 a 4500
001 1061592
005 2020-12-18
008 2020 bl uuuu u00u1 u #d
024 7 $a10.1007/s00436-020-06922-x$2DOI
100 1 $aDORSCH, M.
245 $aMicrosatellite genotyping reveals extensive genetic diversity in bovine Neospora caninum from the humid Pampa region in Argentina.$h[electronic resource]
260 $c2020
500 $aArticle history:Received: 12 August 2020 /Accepted: 5 October 2020
520 $aAbstract: Neospora caninum is an apicomplexan protozoan and a major cause of abortion in cattle worldwide. In the Argentinian Humid Pampa, bovine neosporosis causes severe economic losses. Despite this, information on the genetic structure of N. caninum in this region is limited. Therefore, this study aimed to genetically characterize N. caninum isolates associated with bovine abortion in the Humid Pampa region. For this purpose, spontaneous bovine fetal tissues submitted for diagnosis to the Veterinary Diagnostic Service at INTA Balcarce during 2008?2019 were assessed by PCR, indirect fluorescent antibody test (IFAT), and histologic analysis. PCR-positive samples were tested by multilocus microsatellite genotyping (MLGs) using 9 microsatellite markers. Thirty-one different genotypes were identified from 32 samples with at least seven markers. Argentinian MLGs were grouped into two clonal clusters when analyzed using eBURST network and principal coordinate analysis. No segregation based on the year of collection, animal biotype, or geographic origin was observed. In addition, the presence of linkage disequilibrium supported the clonal propagation of Argentinian MLGs. One Argentinian subpopulation was associated with isolates from Spain, Uruguay, Brazil, and Mexico, and the other one was linked to isolates from Scotland, Spain, and Germany. These findings reveal the presence of two clonal subpopulations of N. caninum in the Humid Pampa.
650 $aARGENTINA
653 $aABORTION
653 $aBOVINE
653 $aMICROSATELLITE MARKERS
653 $aNEOSPORA CANINUM
653 $aPLATAFORMA SALUD ANIMAL
700 1 $aREGIDOR-CERRILLO, J.
700 1 $aORTEGA-MORA, L.M.
700 1 $aSCIOLI, M.V.
700 1 $aMORRELL, E.L.
700 1 $aCANTÓN, G.J.
700 1 $aMOORE, D.P.
700 1 $aHECKER, Y.P.
773 $tParasitology Research, December 2020, Volume 119, Issue 12, Pages 4049-4059. Doi: https://doi-org/10.1007/s00436-020-06922-x
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INIA La Estanzuela (LE) |
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| Acceso al texto completo restringido a Biblioteca INIA Tacuarembó. Por información adicional contacte bibliotb@tb.inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Tacuarembó. |
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Fecha actual : |
09/09/2014 |
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Actualizado : |
04/02/2020 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
CHANVALLON, A.; BLACHE, D.; CHADWICK, A.; ESMAILI, T.; HAWKEN, P.A.R.; MARTIN, G.B.; VIÑOLES, C.; FABRE-NYS, C. |
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Afiliación : |
A. CHANVALLON, Centre Interrégional d’Information et de Recherche en Production Ovine, Site Expérimental du Mourier, 87800 St Priest Ligoure, FranceUMR 6175 INRA Physiologie de la Reproduction et des Comportements - CNRS - Université de Tours - Haras Nationaux, 37380; D. BLACHE, UWA Institute of Agriculture, The University of Western Australia, Crawley 6009, Perth, Australia; A. CHADWICK, UWA Institute of Agriculture, The University of Western Australia, Crawley 6009, Perth, Australia; T. ESMAILI, UWA Institute of Agriculture, The University of Western Australia, Crawley 6009, Perth, Australia; P.A.R. HAWKEN, UWA Institute of Agriculture, The University of Western Australia, Crawley 6009, Perth, Australia; G.B. MARTIN, UWA Institute of Agriculture, The University of Western Australia, Crawley 6009, Perth, Australia; CAROLINA VIÑOLES GIL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; UWA Institute of Agriculture, The University of Western Australia, Crawley 6009, Perth, Australia; C. FABRE-NYS, UMR 6175 INRA Physiologie de la Reproduction et des Comportements - CNRS - Université de Tours - Haras Nationaux, 37380 Nouzilly, France. |
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Título : |
Sexual experience and temperament affect the response of Merino ewes to the ram effect during the anoestrous season. |
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Fecha de publicación : |
2010 |
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Fuente / Imprenta : |
Animal Reproduction Science, 2010, v. 119, p. 205-211. |
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DOI : |
10.1016/j.anireprosci.2010.02.003 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 26 October 2009 // Received in revised form 11 January 2010 // Accepted 4 February 2010 // Available online 11 February 2010. Corresponding author at: : Audrey.Chanvallon@tours.inra.fr // Acknowledgments: This research was supported by the Australian Research Council (Project DP0558952) and by Meat & Livestock Australia (Project MS027 ?LambMax?). We thank Steve Gray for his assistance in the care and management of the animals, and Margaret Blackberry for her assistance with the hormone assays. This work was a part of Audrey Chanvallon?s PhD project and was supported by CIFRE Convention and the ANRT. We thank also the Région Centre. |
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Contenido : |
Abstract: In seasonally anoestrous ewes of many breeds, the introduction of rams triggers an increase in gonadotrophin secretion that induces ovulation, a phenomenon known as the ram effect. The ram effect is a practical method for mating ewes outside the natural breeding season, and also can provide synchronised lambing, but the variability of the response, especially in young animals, reduces its potential for widespread application. The aim of our study was to assess two factors that are thought to contribute to the variability in young ewes: temperament and sexual experience. We used anovulatory ewes from a flock that had been genetically selected for calm or nervous temperament and compared the endocrine and ovarian responses to the ram effect in four groups (each n = 15): calm and parous (3-6 years old); calm and nulliparous (2 years old); nervous and parous; and nervous and nulliparous. Parous ewes, independently of their temperament, exhibited a faster endocrine response and a higher proportion of females cycling after ram introduction than nulliparous ewes. Nervous ewes exhibited a higher proportion of females cycling after ram introduction than calm ewes, but only in the nulliparous group. We conclude that temperament exerts little influence on the response to the ram effect in sexually experienced ewes, and that females of nervous temperament appear to respond better when sexually naive. Both sexual experience and temperament need to be taken into consideration when flock management involves the ram effect. Finally, some ewes were cyclic at ram introduction, yet exhibited an increase in LH secretion even in the presence of high concentrations of progesterone. The mechanism by which the inhibitory effect of progesterone on LH secretion was bypassed needs to be clarified. MenosAbstract: In seasonally anoestrous ewes of many breeds, the introduction of rams triggers an increase in gonadotrophin secretion that induces ovulation, a phenomenon known as the ram effect. The ram effect is a practical method for mating ewes outside the natural breeding season, and also can provide synchronised lambing, but the variability of the response, especially in young animals, reduces its potential for widespread application. The aim of our study was to assess two factors that are thought to contribute to the variability in young ewes: temperament and sexual experience. We used anovulatory ewes from a flock that had been genetically selected for calm or nervous temperament and compared the endocrine and ovarian responses to the ram effect in four groups (each n = 15): calm and parous (3-6 years old); calm and nulliparous (2 years old); nervous and parous; and nervous and nulliparous. Parous ewes, independently of their temperament, exhibited a faster endocrine response and a higher proportion of females cycling after ram introduction than nulliparous ewes. Nervous ewes exhibited a higher proportion of females cycling after ram introduction than calm ewes, but only in the nulliparous group. We conclude that temperament exerts little influence on the response to the ram effect in sexually experienced ewes, and that females of nervous temperament appear to respond better when sexually naive. Both sexual experience and temperament need to be taken into consideration wh... Presentar Todo |
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Palabras claves : |
CYCLICITY; EXPERIENCE; MALE EFFECT; REPRODUCCIÓN; SHEEP; TEMPERAMENT. |
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Thesagro : |
OVINOS. |
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Asunto categoría : |
L01 Ganadería |
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Marc : |
LEADER 03332naa a2200313 a 4500
001 1050084
005 2020-02-04
008 2010 bl uuuu u00u1 u #d
024 7 $a10.1016/j.anireprosci.2010.02.003$2DOI
100 1 $aCHANVALLON, A.
245 $aSexual experience and temperament affect the response of Merino ewes to the ram effect during the anoestrous season.
260 $c2010
500 $aArticle history: Received 26 October 2009 // Received in revised form 11 January 2010 // Accepted 4 February 2010 // Available online 11 February 2010. Corresponding author at: : Audrey.Chanvallon@tours.inra.fr // Acknowledgments: This research was supported by the Australian Research Council (Project DP0558952) and by Meat & Livestock Australia (Project MS027 ?LambMax?). We thank Steve Gray for his assistance in the care and management of the animals, and Margaret Blackberry for her assistance with the hormone assays. This work was a part of Audrey Chanvallon?s PhD project and was supported by CIFRE Convention and the ANRT. We thank also the Région Centre.
520 $aAbstract: In seasonally anoestrous ewes of many breeds, the introduction of rams triggers an increase in gonadotrophin secretion that induces ovulation, a phenomenon known as the ram effect. The ram effect is a practical method for mating ewes outside the natural breeding season, and also can provide synchronised lambing, but the variability of the response, especially in young animals, reduces its potential for widespread application. The aim of our study was to assess two factors that are thought to contribute to the variability in young ewes: temperament and sexual experience. We used anovulatory ewes from a flock that had been genetically selected for calm or nervous temperament and compared the endocrine and ovarian responses to the ram effect in four groups (each n = 15): calm and parous (3-6 years old); calm and nulliparous (2 years old); nervous and parous; and nervous and nulliparous. Parous ewes, independently of their temperament, exhibited a faster endocrine response and a higher proportion of females cycling after ram introduction than nulliparous ewes. Nervous ewes exhibited a higher proportion of females cycling after ram introduction than calm ewes, but only in the nulliparous group. We conclude that temperament exerts little influence on the response to the ram effect in sexually experienced ewes, and that females of nervous temperament appear to respond better when sexually naive. Both sexual experience and temperament need to be taken into consideration when flock management involves the ram effect. Finally, some ewes were cyclic at ram introduction, yet exhibited an increase in LH secretion even in the presence of high concentrations of progesterone. The mechanism by which the inhibitory effect of progesterone on LH secretion was bypassed needs to be clarified.
650 $aOVINOS
653 $aCYCLICITY
653 $aEXPERIENCE
653 $aMALE EFFECT
653 $aREPRODUCCIÓN
653 $aSHEEP
653 $aTEMPERAMENT
700 1 $aBLACHE, D.
700 1 $aCHADWICK, A.
700 1 $aESMAILI, T.
700 1 $aHAWKEN, P.A.R.
700 1 $aMARTIN, G.B.
700 1 $aVIÑOLES, C.
700 1 $aFABRE-NYS, C.
773 $tAnimal Reproduction Science, 2010$gv. 119, p. 205-211.
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