| |
|
|
|
Registro completo
|
|
Biblioteca (s) : |
INIA Las Brujas. |
|
Fecha : |
03/02/2020 |
|
Actualizado : |
20/07/2022 |
|
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
|
Autor : |
MONTENEGRO, M.C.; PERAZA, P.; BALEMIAN, N.; CARBALLO, C.; BARLOCCO, N.; BARRIOS, P.G.; MERNIES, B.; SAADOUN, A.; CASTRO, G.; GUIMARÃES, S.F.; LLAMBÍ, S. |
|
Afiliación : |
M. C. MONTENEGRO, Departamento de Genética y Mejora Animal, Facultad de Veterinaria, Universidad de la República, Montevideo, Uruguay; PABLO PERAZA DOS SANTOS, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; N. BALEMIAN, Departamento de Genética y Mejora Animal, Facultad de Veterinaria, Universidad de la República, Montevideo, Uruguay; C. CARBALLO, Departamento de Producción Animal y Pasturas, Facultad de Agronomía, Universidad de la República, Montevideo, Uruguay; N. BARLOCCO, Departamento de Producción Animal y Pasturas, Facultad de Agronomía, Universidad de la República, Montevideo, Uruguay; P. G. BARRIOS, Departamento de Biometría, Estadística y Cómputo, Facultad de Agronomía, Universidad de la República, Montevideo, Uruguay; B. MERNIES, Departamento de Genética y Mejora Animal, Facultad de Veterinaria, Universidad de la República, Montevideo, Uruguay; A. SAADOUN, Sección Fisiología y Nutrición, Facultad de Ciencias, Universidad de la República, Montevideo, Uruguay; G. CASTRO, Departamento Animales de Granja, Facultad de Veterinaria, Universidad de la República, Montevideo, Uruguay; S.F. GUIMARÃES, Departamento de Zootecnia, Universidade Federal de Viçosa, Minas Gerais, Brasil; S. LLAMBÍ, Departamento de Genética y Mejora Animal, Facultad de Veterinaria, Universidad de la República, Montevideo, Uruguay. |
|
Título : |
Gene expression analysis by RNA-sequencing of Longissimus dorsi muscle of pigs fed diets with differing lipid contents. |
|
Fecha de publicación : |
2019 |
|
Fuente / Imprenta : |
Genetics and Molecular Research, 2019, volume 18, number 4: GMR18307. OPEN ACCESS. Doi: https://doi.org/10.4238/gmr18307 |
|
ISSN : |
1676-5680 |
|
DOI : |
10.4238/gmr18307 |
|
Idioma : |
Inglés |
|
Notas : |
Article history: Received April 05, 2019 / Accepted November 22, 2019 / Published December 31, 2019.
Corresponding Author: M.C. Montenegro - mariadc.montenegro@gmail.com |
|
Contenido : |
ABSTRACT.
We examined the effect of lipid content in the diet on the transcriptome of the Longissimus dorsi muscle in pigs. Our objective was to examine changes at the molecular level affecting economically relevant meat quality characteristics such as intramuscular fat deposition and fatty acid profile. The treatments consisted of isoproteic and isoenergetic diets with differing lipid contents due to addition of rice bran. The control diet (T0) was a normal basic diet and the test diet (T15) had 15% rice bran. The final lipid content (ether extract) in the diets was 3.4 and 4.8% in T0 and T15, respectively. Three male piglets of the Uruguayan creole breed Pampa Rocha were used per treatment, which lasted from weaning at 42 days until 77 days of age. The animals were reared in confinement on deep bedding and were slaughtered at the end of the experiment, when muscle samples were collected. Intramuscular fat content (IMF) and fatty acid composition were analyzed to determine if diets had a phenotypic effect. Gene expression analysis was performed with RNA-seq methodology to carrying out a functional analysis of genes with differential expression between treatments. The added fat to the diet did not affect IMF or fatty acid composition. However, we identified 359 genes with differential expression between treatments.
These genes participate in various metabolic pathways, some of them affecting meat quality. The most relevant genes identified in this regard were PDK4 (up-regulated with T15), which is associated with energy metabolism, FOS, ATF3, MYOD1 and MAFF (all downregulated with T15), which are associated with skeletal muscle growth, and TNC (up-regulated with T15), which is associated with extracellular matrix-receptor interactions. This study of the skeletal muscle transcriptome in pigs can help understand the genetic basis of how diet affects important production traits. MenosABSTRACT.
We examined the effect of lipid content in the diet on the transcriptome of the Longissimus dorsi muscle in pigs. Our objective was to examine changes at the molecular level affecting economically relevant meat quality characteristics such as intramuscular fat deposition and fatty acid profile. The treatments consisted of isoproteic and isoenergetic diets with differing lipid contents due to addition of rice bran. The control diet (T0) was a normal basic diet and the test diet (T15) had 15% rice bran. The final lipid content (ether extract) in the diets was 3.4 and 4.8% in T0 and T15, respectively. Three male piglets of the Uruguayan creole breed Pampa Rocha were used per treatment, which lasted from weaning at 42 days until 77 days of age. The animals were reared in confinement on deep bedding and were slaughtered at the end of the experiment, when muscle samples were collected. Intramuscular fat content (IMF) and fatty acid composition were analyzed to determine if diets had a phenotypic effect. Gene expression analysis was performed with RNA-seq methodology to carrying out a functional analysis of genes with differential expression between treatments. The added fat to the diet did not affect IMF or fatty acid composition. However, we identified 359 genes with differential expression between treatments.
These genes participate in various metabolic pathways, some of them affecting meat quality. The most relevant genes identified in this regard were PDK4 (up-regu... Presentar Todo |
|
Palabras claves : |
Candidate genes; Meat quality; Nutrigenomics; Skeletal muscle; Transcriptomic. |
|
Asunto categoría : |
L10 Genética y mejoramiento animal |
|
URL : |
https://www.geneticsmr.com/sites/default/files/articles/year2019/vol18-4/pdf/gmr18307_-_gene-expression-analysis-rna-sequencing-longissimus-dorsi-muscle-pigs-fed-diets-differing.pdf
|
|
Marc : |
LEADER 03091naa a2200337 a 4500
001 1060743
005 2022-07-20
008 2019 bl uuuu u00u1 u #d
022 $a1676-5680
024 7 $a10.4238/gmr18307$2DOI
100 1 $aMONTENEGRO, M.C.
245 $aGene expression analysis by RNA-sequencing of Longissimus dorsi muscle of pigs fed diets with differing lipid contents.$h[electronic resource]
260 $c2019
500 $aArticle history: Received April 05, 2019 / Accepted November 22, 2019 / Published December 31, 2019. Corresponding Author: M.C. Montenegro - mariadc.montenegro@gmail.com
520 $aABSTRACT. We examined the effect of lipid content in the diet on the transcriptome of the Longissimus dorsi muscle in pigs. Our objective was to examine changes at the molecular level affecting economically relevant meat quality characteristics such as intramuscular fat deposition and fatty acid profile. The treatments consisted of isoproteic and isoenergetic diets with differing lipid contents due to addition of rice bran. The control diet (T0) was a normal basic diet and the test diet (T15) had 15% rice bran. The final lipid content (ether extract) in the diets was 3.4 and 4.8% in T0 and T15, respectively. Three male piglets of the Uruguayan creole breed Pampa Rocha were used per treatment, which lasted from weaning at 42 days until 77 days of age. The animals were reared in confinement on deep bedding and were slaughtered at the end of the experiment, when muscle samples were collected. Intramuscular fat content (IMF) and fatty acid composition were analyzed to determine if diets had a phenotypic effect. Gene expression analysis was performed with RNA-seq methodology to carrying out a functional analysis of genes with differential expression between treatments. The added fat to the diet did not affect IMF or fatty acid composition. However, we identified 359 genes with differential expression between treatments. These genes participate in various metabolic pathways, some of them affecting meat quality. The most relevant genes identified in this regard were PDK4 (up-regulated with T15), which is associated with energy metabolism, FOS, ATF3, MYOD1 and MAFF (all downregulated with T15), which are associated with skeletal muscle growth, and TNC (up-regulated with T15), which is associated with extracellular matrix-receptor interactions. This study of the skeletal muscle transcriptome in pigs can help understand the genetic basis of how diet affects important production traits.
653 $aCandidate genes
653 $aMeat quality
653 $aNutrigenomics
653 $aSkeletal muscle
653 $aTranscriptomic
700 1 $aPERAZA, P.
700 1 $aBALEMIAN, N.
700 1 $aCARBALLO, C.
700 1 $aBARLOCCO, N.
700 1 $aBARRIOS, P.G.
700 1 $aMERNIES, B.
700 1 $aSAADOUN, A.
700 1 $aCASTRO, G.
700 1 $aGUIMARÃES, S.F.
700 1 $aLLAMBÍ, S.
773 $tGenetics and Molecular Research, 2019, volume 18, number 4: GMR18307. OPEN ACCESS. Doi: https://doi.org/10.4238/gmr18307
Descargar
Esconder MarcPresentar Marc Completo |
|
Registro original : |
INIA Las Brujas (LB) |
|
|
Biblioteca
|
Identificación
|
Origen
|
Tipo / Formato
|
Clasificación
|
Cutter
|
Registro
|
Volumen
|
Estado
|
Volver
|
|
|
|
Registro completo
|
|
Biblioteca (s) : |
INIA Treinta y Tres. |
|
Fecha actual : |
02/02/2017 |
|
Actualizado : |
02/02/2017 |
|
Tipo de producción científica : |
Artículos en Revistas Agropecuarias |
|
Autor : |
MACEDO, I.; CASTILLO, J.; SALDAIN, N.E.; MARTÍNEZ, S.; AYALA, W.; HERNANDEZ, J.; SERRON, N.; BORDAGORRI, P.; ZORRILLA DE SAN MARTÍN, G.; TERRA, J.A. |
|
Afiliación : |
IGNACIO MACEDO YAPOR, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; EMILSE JESUS CASTILLO VELAZQUEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; NÉSTOR ELIO SALDAIN CROCCE, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; SEBASTIÁN MARTÍNEZ KOPP, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; WALTER FELIZARDO AYALA SILVERA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JORGE FABIAN HERNANDEZ FERREIRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; NESTOR DANIEL SERRON DUPLATT, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; PIERRE ALEXANDER BORDAGORRI ORREGO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; GONZALO ROBERTO ZORRILLA DE SAN MARTÍN PEREYRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JOSÉ ALFREDO TERRA FERNÁNDEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
|
Título : |
Nuevas rotaciones arroceras: primeros datos de productividad |
|
Fecha de publicación : |
2016 |
|
Fuente / Imprenta : |
Arroz, 2016, v.16, no. 88, p. 34-39. |
|
Idioma : |
Español |
|
Thesagro : |
ARROZ; PASTURAS; ROTACIONES; SOJA; SORGO. |
|
Asunto categoría : |
F01 Cultivo |
|
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/6457/1/Arroz-2016-88-Macedo.pdf
|
|
Marc : |
LEADER 00692naa a2200277 a 4500
001 1056620
005 2017-02-02
008 2016 bl uuuu u00u1 u #d
100 1 $aMACEDO, I.
245 $aNuevas rotaciones arroceras$bprimeros datos de productividad
260 $c2016
650 $aARROZ
650 $aPASTURAS
650 $aROTACIONES
650 $aSOJA
650 $aSORGO
700 1 $aCASTILLO, J.
700 1 $aSALDAIN, N.E.
700 1 $aMARTÍNEZ, S.
700 1 $aAYALA, W.
700 1 $aHERNANDEZ, J.
700 1 $aSERRON, N.
700 1 $aBORDAGORRI, P.
700 1 $aZORRILLA DE SAN MARTÍN, G.
700 1 $aTERRA, J.A.
773 $tArroz, 2016$gv.16, no. 88, p. 34-39.
Descargar
Esconder MarcPresentar Marc Completo |
|
Registro original : |
INIA Treinta y Tres (TT) |
|
|
Biblioteca
|
Identificación
|
Origen
|
Tipo / Formato
|
Clasificación
|
Cutter
|
Registro
|
Volumen
|
Estado
|
Volver
|
| Expresión de búsqueda válido. Check! |
|
|
