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Registro completo
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Biblioteca (s) : |
INIA Treinta y Tres. |
Fecha : |
08/01/2021 |
Actualizado : |
08/01/2021 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
RIVAS, F.; HAMPTON, J.G.; ALTIER, N.; SWAMINATHAN, J.; ROSTÁS, M.; WESSMAN, P.; SAVILLE, D. J.; JACKSON, T. A.; JACKSON, M. A.; GLARE, T. R. |
Afiliación : |
FEDERICO RIVAS FRANCO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay // Bio-Protection Research Center, Lincoln University, Lincoln, New Zealand.; JOHN G. HAMPTON, Bio-Protection Research Center, Lincoln University, New Zealand.; NORA ADRIANA ALTIER MANZINI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JAYANTHI SWAMINATHAN, Faculty of Agriculture and Life Sciences, Lincoln University, New Zealand.; MICHAEL ROSTÁS, Bio-Protection Research Center, Lincoln University, New Zealand.; PER WESSMAN, Department Chemical Process and Pharmaceutical Development, Unit Formulation, Research Institute os Sweden (RISE), Sweden.; DAVID J. SAVILLE, Savile Statistical Consulting Limited, Lincoln, New Zealand.; TREVOR A. JACKSON, AgResearch, Lincoln Research Center, Lincoln, New Zealand.; MARK A. JACKSON, United State Department of Agriculture (USDA), National Center for Agricultural Utilization, Peoria, IL, USA.; TRAVIS R. GLARE, Bio-Protection Research Center, Lincoln University, Lincoln, New Zealand. |
Título : |
Production of Microsclerotia from entomopathogenic fungi and use in maize seed coating as delivery for biocontrol against Fusarim graminearum. |
Fecha de publicación : |
2020 |
Fuente / Imprenta : |
Frontiers in Sustainable Food Systems, December, 2020, volume 4, Article number 606828, Pages 1-13.Open Access. Doi: https://doi.org/10.3389/fsufs.2020.606828 |
DOI : |
10.3389/fsufs.2020.606828 |
Idioma : |
Inglés |
Notas : |
Article history: Received: 15 September 2020 / Accepted: 30 October 2020 / Published: 10 December 2020.
Correspondence: Federico Rivas-Franco frivas@inia.org.uy |
Contenido : |
The commercial use of the entomopathogenic fungi Metarhizium spp. in biopesticides has gained more interest since the discovery that several species of this genus are able to colonize roots. In general, commercial products with Metarhizium are formulated based on conidia for insect pest control. The process of mass production, harvesting, and formulation of infective conidia can be detrimental for conidial viability. Entomopathogenic fungi such as Metarhizium spp. are able to produce high concentrations of resistant structures, known as microsclerotia, when grown in liquid media. Microsclerotia are desiccation tolerant, with excellent storage stability, and are capable of producing high quantities of infective conidia after rehydration. The aim of this study was to evaluate microsclerotia production by different isolates of Metarhizium spp. and determine the effect of microsclerotia coated onto maize seeds on plant growth in the presence of soil-borne pathogen Fusarium graminearum. On average, ~1 × 105 microsclerotia/mL were produced by selected isolates of M. anisopliae (A1080 and F672) and Metarhizium robertsii (F447). Microsclerotia were formulated as granules with diatomaceous earth and used for seed coating, after which propagules produced around 5 × 106 CFU/g of seeds. In the presence of the plant pathogen, maize plants grown from untreated seeds had the lowest growth, while plants treated with the Metarhizium microsclerotia had significantly greater growth than the control plants. Hyphae were observed growing on and in root tissues in all the Metarhizium spp. treatments but not in samples from control plants. Metarhizium hyphal penetration points' on roots were observed 1 month after sowing, indicating the fungi were colonizing roots as endophytes. The results obtained indicate that microsclerotia can be coated onto seeds, providing plant protection against soil plant pathogens and a method to establish Metarhizium in the ecto- and endo-rhizosphere of maize roots, allowing the persistence of this biocontrol agent. MenosThe commercial use of the entomopathogenic fungi Metarhizium spp. in biopesticides has gained more interest since the discovery that several species of this genus are able to colonize roots. In general, commercial products with Metarhizium are formulated based on conidia for insect pest control. The process of mass production, harvesting, and formulation of infective conidia can be detrimental for conidial viability. Entomopathogenic fungi such as Metarhizium spp. are able to produce high concentrations of resistant structures, known as microsclerotia, when grown in liquid media. Microsclerotia are desiccation tolerant, with excellent storage stability, and are capable of producing high quantities of infective conidia after rehydration. The aim of this study was to evaluate microsclerotia production by different isolates of Metarhizium spp. and determine the effect of microsclerotia coated onto maize seeds on plant growth in the presence of soil-borne pathogen Fusarium graminearum. On average, ~1 × 105 microsclerotia/mL were produced by selected isolates of M. anisopliae (A1080 and F672) and Metarhizium robertsii (F447). Microsclerotia were formulated as granules with diatomaceous earth and used for seed coating, after which propagules produced around 5 × 106 CFU/g of seeds. In the presence of the plant pathogen, maize plants grown from untreated seeds had the lowest growth, while plants treated with the Metarhizium microsclerotia had significantly greater growth than the co... Presentar Todo |
Palabras claves : |
BIOCONTROL; ENDOPHYTIC ENTOMOPATHOGENIC FUNGI; FUSARIUM GRAMINEARUM; METARHIZIUM SPP; MICROSCLEROTIA; PLANT GROWTH PROMOTION; PLANT PROTECTION; SEED COATING. |
Asunto categoría : |
H20 Enfermedades de las plantas |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/14918/1/fsufs-04-606828-Rivas.pdf
https://www.frontiersin.org/articles/10.3389/fsufs.2020.606828/full
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Marc : |
LEADER 03370naa a2200349 a 4500 001 1061656 005 2021-01-08 008 2020 bl uuuu u00u1 u #d 024 7 $a10.3389/fsufs.2020.606828$2DOI 100 1 $aRIVAS, F. 245 $aProduction of Microsclerotia from entomopathogenic fungi and use in maize seed coating as delivery for biocontrol against Fusarim graminearum.$h[electronic resource] 260 $c2020 500 $aArticle history: Received: 15 September 2020 / Accepted: 30 October 2020 / Published: 10 December 2020. Correspondence: Federico Rivas-Franco frivas@inia.org.uy 520 $aThe commercial use of the entomopathogenic fungi Metarhizium spp. in biopesticides has gained more interest since the discovery that several species of this genus are able to colonize roots. In general, commercial products with Metarhizium are formulated based on conidia for insect pest control. The process of mass production, harvesting, and formulation of infective conidia can be detrimental for conidial viability. Entomopathogenic fungi such as Metarhizium spp. are able to produce high concentrations of resistant structures, known as microsclerotia, when grown in liquid media. Microsclerotia are desiccation tolerant, with excellent storage stability, and are capable of producing high quantities of infective conidia after rehydration. The aim of this study was to evaluate microsclerotia production by different isolates of Metarhizium spp. and determine the effect of microsclerotia coated onto maize seeds on plant growth in the presence of soil-borne pathogen Fusarium graminearum. On average, ~1 × 105 microsclerotia/mL were produced by selected isolates of M. anisopliae (A1080 and F672) and Metarhizium robertsii (F447). Microsclerotia were formulated as granules with diatomaceous earth and used for seed coating, after which propagules produced around 5 × 106 CFU/g of seeds. In the presence of the plant pathogen, maize plants grown from untreated seeds had the lowest growth, while plants treated with the Metarhizium microsclerotia had significantly greater growth than the control plants. Hyphae were observed growing on and in root tissues in all the Metarhizium spp. treatments but not in samples from control plants. Metarhizium hyphal penetration points' on roots were observed 1 month after sowing, indicating the fungi were colonizing roots as endophytes. The results obtained indicate that microsclerotia can be coated onto seeds, providing plant protection against soil plant pathogens and a method to establish Metarhizium in the ecto- and endo-rhizosphere of maize roots, allowing the persistence of this biocontrol agent. 653 $aBIOCONTROL 653 $aENDOPHYTIC ENTOMOPATHOGENIC FUNGI 653 $aFUSARIUM GRAMINEARUM 653 $aMETARHIZIUM SPP 653 $aMICROSCLEROTIA 653 $aPLANT GROWTH PROMOTION 653 $aPLANT PROTECTION 653 $aSEED COATING 700 1 $aHAMPTON, J.G. 700 1 $aALTIER, N. 700 1 $aSWAMINATHAN, J. 700 1 $aROSTÁS, M. 700 1 $aWESSMAN, P. 700 1 $aSAVILLE, D. J. 700 1 $aJACKSON, T. A. 700 1 $aJACKSON, M. A. 700 1 $aGLARE, T. R. 773 $tFrontiers in Sustainable Food Systems, December, 2020, volume 4, Article number 606828, Pages 1-13.Open Access. Doi: https://doi.org/10.3389/fsufs.2020.606828
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INIA Treinta y Tres (TT) |
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| Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha actual : |
10/09/2014 |
Actualizado : |
15/10/2019 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Circulación / Nivel : |
A - 1 |
Autor : |
ALTESOR, P.; GARCÍA, A.; FONT, E.; RODRÍGUEZ-HARALAMBIDES, A.; VILARO, F.; OESTERHELD, M.; SOLER, R.; GONZÁLEZ, A. |
Afiliación : |
FRANCISCO LUIS VILARO PAREJA, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. |
Título : |
Glycoalkaloids of Wild and Cultivated Solanum: Effects on Specialist and Generalist Insect Herbivores. |
Fecha de publicación : |
2014 |
Fuente / Imprenta : |
Journal of Chemical Ecology, 2014, v.40, no.6, p.599-608. |
ISSN : |
0098-0331 |
DOI : |
10.1007/s10886-014-0447-8 |
Idioma : |
Inglés |
Notas : |
Article history: Received: 25 March 2014 /Revised: 5 May 2014 /Accepted: 9 May 2014 / Published online: 27 May 2014. |
Contenido : |
ABSTRACT.
lant domestication by selective breeding may reduce plant chemical defense in favor of growth. However, few studies have simultaneously studied the defensive chemistry of cultivated plants and their wild congeners in connection to herbivore susceptibility. We compared the constitutive glycoalkaloids (GAs) of cultivated potato, Solanum tuberosum, and a wild congener, S. commersonii, by liquid chromatography coupled to mass spectrometry. We also determined the major herbivores present on the two species in field plots, and tested their preference for the plants and their isolated GAs in two-choice bioassays. Solanum commersonii
had a different GA profile and higher concentrations than S. tuberosum. In the field, S. tuberosum was mostly attacked by the generalist aphids Myzus persicae and Macrosiphum euphorbiae, and by the specialist flea beetle Epitrix argentinensis. In contrast, the most common herbivore on S. commersonii was the specialist sawfly Tequus sp. Defoliation levels were higher on the wild species, probably due to the chewing feeding behavior of Tequus sp. As seen in the field, M. persicae and E. argentinensis preferred leaf disks of the cultivated plant, while Tequus sp. preferred those of the wild one. Congruently, GAs from S. commersonii were
avoided by M. persicae and preferred by Tequus sp. The potato aphid performed well on both species and was not deterred by S. commersonii GAs. These observations suggest that different GA profiles explain the feeding preferences of the different herbivores, and that domestication has altered the defensive capacity of S. tuberosum. However, the wild relative is still subject to severe defoliation by a specialist herbivore that may cue on the GAs.
© 2014 Springer Science+Business Media New York. MenosABSTRACT.
lant domestication by selective breeding may reduce plant chemical defense in favor of growth. However, few studies have simultaneously studied the defensive chemistry of cultivated plants and their wild congeners in connection to herbivore susceptibility. We compared the constitutive glycoalkaloids (GAs) of cultivated potato, Solanum tuberosum, and a wild congener, S. commersonii, by liquid chromatography coupled to mass spectrometry. We also determined the major herbivores present on the two species in field plots, and tested their preference for the plants and their isolated GAs in two-choice bioassays. Solanum commersonii
had a different GA profile and higher concentrations than S. tuberosum. In the field, S. tuberosum was mostly attacked by the generalist aphids Myzus persicae and Macrosiphum euphorbiae, and by the specialist flea beetle Epitrix argentinensis. In contrast, the most common herbivore on S. commersonii was the specialist sawfly Tequus sp. Defoliation levels were higher on the wild species, probably due to the chewing feeding behavior of Tequus sp. As seen in the field, M. persicae and E. argentinensis preferred leaf disks of the cultivated plant, while Tequus sp. preferred those of the wild one. Congruently, GAs from S. commersonii were
avoided by M. persicae and preferred by Tequus sp. The potato aphid performed well on both species and was not deterred by S. commersonii GAs. These observations suggest that different GA profiles explain the fee... Presentar Todo |
Palabras claves : |
GLICOALCALOIDES; MECANISMOS DE DEFENSA; REACCIONES DE DEFENSAS DE PLANTAS; SOLANUM. |
Thesagro : |
DOMESTICACIÓN DE PLANTAS. |
Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
Marc : |
LEADER 02793naa a2200301 a 4500 001 1050125 005 2019-10-15 008 2014 bl uuuu u00u1 u #d 022 $a0098-0331 024 7 $a10.1007/s10886-014-0447-8$2DOI 100 1 $aALTESOR, P. 245 $aGlycoalkaloids of Wild and Cultivated Solanum$bEffects on Specialist and Generalist Insect Herbivores.$h[electronic resource] 260 $c2014 500 $aArticle history: Received: 25 March 2014 /Revised: 5 May 2014 /Accepted: 9 May 2014 / Published online: 27 May 2014. 520 $aABSTRACT. lant domestication by selective breeding may reduce plant chemical defense in favor of growth. However, few studies have simultaneously studied the defensive chemistry of cultivated plants and their wild congeners in connection to herbivore susceptibility. We compared the constitutive glycoalkaloids (GAs) of cultivated potato, Solanum tuberosum, and a wild congener, S. commersonii, by liquid chromatography coupled to mass spectrometry. We also determined the major herbivores present on the two species in field plots, and tested their preference for the plants and their isolated GAs in two-choice bioassays. Solanum commersonii had a different GA profile and higher concentrations than S. tuberosum. In the field, S. tuberosum was mostly attacked by the generalist aphids Myzus persicae and Macrosiphum euphorbiae, and by the specialist flea beetle Epitrix argentinensis. In contrast, the most common herbivore on S. commersonii was the specialist sawfly Tequus sp. Defoliation levels were higher on the wild species, probably due to the chewing feeding behavior of Tequus sp. As seen in the field, M. persicae and E. argentinensis preferred leaf disks of the cultivated plant, while Tequus sp. preferred those of the wild one. Congruently, GAs from S. commersonii were avoided by M. persicae and preferred by Tequus sp. The potato aphid performed well on both species and was not deterred by S. commersonii GAs. These observations suggest that different GA profiles explain the feeding preferences of the different herbivores, and that domestication has altered the defensive capacity of S. tuberosum. However, the wild relative is still subject to severe defoliation by a specialist herbivore that may cue on the GAs. © 2014 Springer Science+Business Media New York. 650 $aDOMESTICACIÓN DE PLANTAS 653 $aGLICOALCALOIDES 653 $aMECANISMOS DE DEFENSA 653 $aREACCIONES DE DEFENSAS DE PLANTAS 653 $aSOLANUM 700 1 $aGARCÍA, A. 700 1 $aFONT, E. 700 1 $aRODRÍGUEZ-HARALAMBIDES, A. 700 1 $aVILARO, F. 700 1 $aOESTERHELD, M. 700 1 $aSOLER, R. 700 1 $aGONZÁLEZ, A. 773 $tJournal of Chemical Ecology, 2014$gv.40, no.6, p.599-608.
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