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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
07/03/2023 |
Actualizado : |
07/03/2023 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
MUSSIO, P.; MARTÍNEZ, I.; LUZARDO, S.; NAVARRO, A.; LEOTTA, G.; VARELA, G. |
Afiliación : |
PAULA MUSSIO, Departamento de Microbiología, Laboratorio Tecnológico del Uruguay, Montevideo, Uruguay; INÉS MARTÍNEZ, Latitud, Fundación LATU, Montevideo, Uruguay; SANTIAGO FELIPE LUZARDO VILLAR, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ARMANDO NAVARRO, Departamento de Salud Pública, Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico City, Mexico; GERARDO LEOTTA, Instituto de Ciencia y Tecnología de Sistemas Alimentarios Sustentables, UEDD INTA-CONICET, Buenos Aires, Argentina; GUSTAVO VARELA, Departamento de Bacteriología y Virología, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay. |
Título : |
Phenotypic and genotypic characterization of Shiga toxin-producing Escherichia coli strains recovered from bovine carcasses in Uruguay. |
Complemento del título : |
Original research. |
Fecha de publicación : |
2023 |
Fuente / Imprenta : |
Frontiers in Microbiology, 2023, volume 14, article 1130170. OPEN ACCESS. doi: https://doi.org/10.3389/fmicb.2023.1130170 |
DOI : |
10.3389/fmicb.2023.1130170 |
Idioma : |
Inglés |
Notas : |
Article history: Received 22 December 2022; Accepted 13 February 2023; Published 06 March 2023. -- Correspondence authors: Paula Mussio, email: paumussio@gmail.com ; Gustavo Varela, email: gvarela@higiene.edu.uy -- Edited by: Vinicius Castro, University of Lethbridge, Canada. -- Specialty Section:
This article was submitted to Food Microbiology, a section of the journal Frontiers in Microbiology. -- This article is part of the Research Topic
From Farm Gate to Food Plate: Current Challenges in Foodborne Microorganism Detection, Epidemiology, and Genetic Diversity (https://www.frontiersin.org/research-topics/49842/from-farm-gate-to-food-plate-current-challenges-in-foodborne-microorganism-detection-epidemiology-and-genetic-diversity#articles ). -- LICCENSE: This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY), (http://creativecommons.org/licenses/by/4.0/ ). -- Supplementary material: The Supplementary material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fmicb.2023.1130170/full#supplementary-material . -- FUNDING: This work was supported by Agencia Nacional de Investigación e Innovación (ANII), Fondo Sectorial Innovagro - Inocuidad, Grant: FSA_I_2017_1_140224. |
Contenido : |
Introduction: Shiga toxin-producing Escherichia coli (STEC) is a zoonotic pathogen that cause food-borne diseases in humans. Cattle and derived foodstuffs play a known role as reservoir and vehicles, respectively. In Uruguay, information about the characteristics of circulating STEC in meat productive chain is scarce. The aim was to characterize STEC strains recovered from 800 bovine carcasses of different slaughterhouses. Methods: To characterize STEC strains we use classical microbiological procedures, Whole Genome Sequencing (WGS) and FAO/WHO risk criteria. |
Palabras claves : |
Bovine carcasses; Shiga toxin-producing Escherichia coli (STEC); URUGUAY; Virulence factors; Whole Genome Sequencing (WGS); Zoonotic pathogen. |
Asunto categoría : |
L40 Estructura animal |
URL : |
https://www.frontiersin.org/articles/10.3389/fmicb.2023.1130170/pdf
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Marc : |
LEADER 02780naa a2200277 a 4500 001 1063967 005 2023-03-07 008 2023 bl uuuu u00u1 u #d 024 7 $a10.3389/fmicb.2023.1130170$2DOI 100 1 $aMUSSIO, P. 245 $aPhenotypic and genotypic characterization of Shiga toxin-producing Escherichia coli strains recovered from bovine carcasses in Uruguay.$h[electronic resource] 260 $c2023 500 $aArticle history: Received 22 December 2022; Accepted 13 February 2023; Published 06 March 2023. -- Correspondence authors: Paula Mussio, email: paumussio@gmail.com ; Gustavo Varela, email: gvarela@higiene.edu.uy -- Edited by: Vinicius Castro, University of Lethbridge, Canada. -- Specialty Section: This article was submitted to Food Microbiology, a section of the journal Frontiers in Microbiology. -- This article is part of the Research Topic From Farm Gate to Food Plate: Current Challenges in Foodborne Microorganism Detection, Epidemiology, and Genetic Diversity (https://www.frontiersin.org/research-topics/49842/from-farm-gate-to-food-plate-current-challenges-in-foodborne-microorganism-detection-epidemiology-and-genetic-diversity#articles ). -- LICCENSE: This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY), (http://creativecommons.org/licenses/by/4.0/ ). -- Supplementary material: The Supplementary material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fmicb.2023.1130170/full#supplementary-material . -- FUNDING: This work was supported by Agencia Nacional de Investigación e Innovación (ANII), Fondo Sectorial Innovagro - Inocuidad, Grant: FSA_I_2017_1_140224. 520 $aIntroduction: Shiga toxin-producing Escherichia coli (STEC) is a zoonotic pathogen that cause food-borne diseases in humans. Cattle and derived foodstuffs play a known role as reservoir and vehicles, respectively. In Uruguay, information about the characteristics of circulating STEC in meat productive chain is scarce. The aim was to characterize STEC strains recovered from 800 bovine carcasses of different slaughterhouses. Methods: To characterize STEC strains we use classical microbiological procedures, Whole Genome Sequencing (WGS) and FAO/WHO risk criteria. 653 $aBovine carcasses 653 $aShiga toxin-producing Escherichia coli (STEC) 653 $aURUGUAY 653 $aVirulence factors 653 $aWhole Genome Sequencing (WGS) 653 $aZoonotic pathogen 700 1 $aMARTÍNEZ, I. 700 1 $aLUZARDO, S. 700 1 $aNAVARRO, A. 700 1 $aLEOTTA, G. 700 1 $aVARELA, G. 773 $tFrontiers in Microbiology, 2023, volume 14, article 1130170. OPEN ACCESS. doi: https://doi.org/10.3389/fmicb.2023.1130170
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| Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha actual : |
16/08/2019 |
Actualizado : |
16/08/2019 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Circulación / Nivel : |
Internacional - -- |
Autor : |
HERNÁNDEZ-RODRÍGUEZ, L.; RAMOS-GONZÁLEZ, P. L.; SISTACHS-VEGA, V.; ZAMORA-RODRÍGUEZ, V.; BATISTA-LE RIVEREND, L.; RAMOS-LEAL, M.; PEÑA-BÁRZAGA, I.; LLANES-ALVAREZ, Y. |
Afiliación : |
LESTER HERNÁNDEZ RODRÍGUEZ, Instituto de Investigaciones en Fruticultura Tropical (IIFT), Cuba; INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay.; P. L. RAMOS GONZÁLEZ, Instituto de Investigaciones en Fruticultura Tropical (IIFT), Cuba; Instituto Biológico de São Paulo, Brazil.; V. SISTACHS-VEGA, Statistics Department, Faculty of Mathematics, Universidad de La Habana, Cuba.; V. ZAMORA-RODRI?GUEZ, Instituto de Investigaciones en Fruticultura Tropical (IIFT), Cuba.; L. BATISTA-LE RIVEREND, Instituto de Investigaciones en Fruticultura Tropical (IIFT), Cuba; M. RAMOS-LEAL, Department, Faculty of Mathematics, Universidad de La Habana, Cuba.; I. PEN?A-BA?RZAGA, Instituto de Investigaciones en Fruticultura Tropical (IIFT), Cuba.; Y. LLANES-ALVAREZ, Instituto de Investigaciones en Fruticultura Tropical (IIFT), Cuba. |
Título : |
The viral complex associated with mealybug wilt disease of pineapple in Cuba. |
Fecha de publicación : |
2019 |
Fuente / Imprenta : |
Acta Horticulturae, 30 April 2019, Volume 1239, pages 203-212. |
Serie : |
(Acta Horticulturae; 1239). |
ISBN : |
978-94-62612-36-5 |
ISSN : |
0567-7572 (print) // 2406-6168 (electronic) |
DOI : |
10.17660/ActaHortic.2019.1239.25 |
Idioma : |
Inglés |
Notas : |
Article history: Published 30 april 2019. In: Acta Horticulturae (ISHS) 1239: IX International Pineapple Symposium. Editors: D.P. Bartholomew, D.H. Reinhardt, F.V. Duarte Souza. Convener: J. Valdés-Infante Herrero .
Funding text: Part of these results are included in the Ph.D. thesis document of Lester Hernández-Rodrı́guez, |
Contenido : |
ABSTRACT.
Mealybug wilt of pineapple (MWP) disease has been associated with the infection by a complex of ampeloviruses (Pineapple mealybug wilt-associated virus 1 (PMWaV-1), PMWaV-2, PMWaV-3, PMWaV-4 and PMWaV-5)) (Closteroviridae) and badnaviruses (Pineapple bacilliform CO virus (PBCOV) and Pineapple bacilliform ER virus (PBERV) (Caulimoviridae)). In Cuba, PMWaV-1, PMWaV-2, PMWaV-3 and PBCOV are widespread in commercial pineapple fields, but the association of these viruses with the aetiology of the disease remains unknown. During 2009 to 2012, a survey to detect PMWaV-1, PMWaV-2 and PMWaV-3 by RT-PCR, and PBCOV by non-radioactive Dot Blot hybridization was carried out on samples collected from asymptomatic or MWP symptomatic pineapple plants from 24 commercial fields in 10 provinces and Isla de la Juventud. PMWaV-1, PMWaV-2, PMWaV-3, and PBCOV occurred in simple or mixed infections, whereas PMWaV-2 was the viral species most frequently detected in symptomatic plants. The positive or negative diagnostic results of the RT-PCR to detect PMWaV-2 was more reliably associated with the MWP symptomatic or asymptomatic status of the sampled plants, respectively, than those from PMWaV-1, PMWaV-3 or PBCOV detection techniques. This work discloses the correlation between the infection by PMWaV-2 and the presence of MWP symptoms in Cuba, supporting previous studies indicating the major role of this virus triggering MWP disease.
© 2019 International Society for Horticultural Science. All rights reserved. MenosABSTRACT.
Mealybug wilt of pineapple (MWP) disease has been associated with the infection by a complex of ampeloviruses (Pineapple mealybug wilt-associated virus 1 (PMWaV-1), PMWaV-2, PMWaV-3, PMWaV-4 and PMWaV-5)) (Closteroviridae) and badnaviruses (Pineapple bacilliform CO virus (PBCOV) and Pineapple bacilliform ER virus (PBERV) (Caulimoviridae)). In Cuba, PMWaV-1, PMWaV-2, PMWaV-3 and PBCOV are widespread in commercial pineapple fields, but the association of these viruses with the aetiology of the disease remains unknown. During 2009 to 2012, a survey to detect PMWaV-1, PMWaV-2 and PMWaV-3 by RT-PCR, and PBCOV by non-radioactive Dot Blot hybridization was carried out on samples collected from asymptomatic or MWP symptomatic pineapple plants from 24 commercial fields in 10 provinces and Isla de la Juventud. PMWaV-1, PMWaV-2, PMWaV-3, and PBCOV occurred in simple or mixed infections, whereas PMWaV-2 was the viral species most frequently detected in symptomatic plants. The positive or negative diagnostic results of the RT-PCR to detect PMWaV-2 was more reliably associated with the MWP symptomatic or asymptomatic status of the sampled plants, respectively, than those from PMWaV-1, PMWaV-3 or PBCOV detection techniques. This work discloses the correlation between the infection by PMWaV-2 and the presence of MWP symptoms in Cuba, supporting previous studies indicating the major role of this virus triggering MWP disease.
© 2019 International Society for Horticultural Science. ... Presentar Todo |
Palabras claves : |
AMPELOVIRUS; ANANAS COMOSUS; BADNAVIRUS; CAULIMOVIRIDAE; CLOSTEROVIRIDAE; MWP; PBCOV; PMWaV. |
Asunto categoría : |
F01 Cultivo |
Marc : |
LEADER 02929naa a2200361 a 4500 001 1060012 005 2019-08-16 008 2019 bl uuuu u00u1 u #d 020 $a978-94-62612-36-5 022 $a0567-7572 (print) // 2406-6168 (electronic) 024 7 $a10.17660/ActaHortic.2019.1239.25$2DOI 100 1 $aHERNÁNDEZ-RODRÍGUEZ, L. 245 $aThe viral complex associated with mealybug wilt disease of pineapple in Cuba.$h[electronic resource] 260 $c2019 490 $a(Acta Horticulturae; 1239). 500 $aArticle history: Published 30 april 2019. In: Acta Horticulturae (ISHS) 1239: IX International Pineapple Symposium. Editors: D.P. Bartholomew, D.H. Reinhardt, F.V. Duarte Souza. Convener: J. Valdés-Infante Herrero . Funding text: Part of these results are included in the Ph.D. thesis document of Lester Hernández-Rodrı́guez, 520 $aABSTRACT. Mealybug wilt of pineapple (MWP) disease has been associated with the infection by a complex of ampeloviruses (Pineapple mealybug wilt-associated virus 1 (PMWaV-1), PMWaV-2, PMWaV-3, PMWaV-4 and PMWaV-5)) (Closteroviridae) and badnaviruses (Pineapple bacilliform CO virus (PBCOV) and Pineapple bacilliform ER virus (PBERV) (Caulimoviridae)). In Cuba, PMWaV-1, PMWaV-2, PMWaV-3 and PBCOV are widespread in commercial pineapple fields, but the association of these viruses with the aetiology of the disease remains unknown. During 2009 to 2012, a survey to detect PMWaV-1, PMWaV-2 and PMWaV-3 by RT-PCR, and PBCOV by non-radioactive Dot Blot hybridization was carried out on samples collected from asymptomatic or MWP symptomatic pineapple plants from 24 commercial fields in 10 provinces and Isla de la Juventud. PMWaV-1, PMWaV-2, PMWaV-3, and PBCOV occurred in simple or mixed infections, whereas PMWaV-2 was the viral species most frequently detected in symptomatic plants. The positive or negative diagnostic results of the RT-PCR to detect PMWaV-2 was more reliably associated with the MWP symptomatic or asymptomatic status of the sampled plants, respectively, than those from PMWaV-1, PMWaV-3 or PBCOV detection techniques. This work discloses the correlation between the infection by PMWaV-2 and the presence of MWP symptoms in Cuba, supporting previous studies indicating the major role of this virus triggering MWP disease. © 2019 International Society for Horticultural Science. All rights reserved. 653 $aAMPELOVIRUS 653 $aANANAS COMOSUS 653 $aBADNAVIRUS 653 $aCAULIMOVIRIDAE 653 $aCLOSTEROVIRIDAE 653 $aMWP 653 $aPBCOV 653 $aPMWaV 700 1 $aRAMOS-GONZÁLEZ, P. L. 700 1 $aSISTACHS-VEGA, V. 700 1 $aZAMORA-RODRÍGUEZ, V. 700 1 $aBATISTA-LE RIVEREND, L. 700 1 $aRAMOS-LEAL, M. 700 1 $aPEÑA-BÁRZAGA, I. 700 1 $aLLANES-ALVAREZ, Y. 773 $tActa Horticulturae, 30 April 2019, Volume 1239, pages 203-212.
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