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Registro completo
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Biblioteca (s) : |
INIA Treinta y Tres. |
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Fecha : |
22/01/2021 |
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Actualizado : |
14/04/2021 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
DELPIAZZO, R.; BARCELLOS, M.; BARROS, S.; BENTANCOR, L.; FRAGA, M.; GIL, J.; IRAOLA, G.; MORSELLA, C.; PAOLICCHI, F.; PÉREZ, R.; RIET-CORREA, F.; SANGUINETTI, M.; SILVA, A.; SILVEIRA, C.S.; CALLEROS, L. |
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Afiliación : |
RAFAEL DELPIAZZO, Universidad de la República Oriental del Uruguay. Facultad de Veterinaria. Estación Experimental "Dr. Mario A. Cassinoni". Departamento de Salud de los Sistemas Pecuarios. Paysandú, Uruguay.; MAILA BARCELLOS, Universidad de la República Oriental del Uruguay. Facultad de Ciencias. Sección Genética Evolutiva. Montevideo, Uruguay.; SOFÍA BARROS, Universidad de la República Oriental del Uruguay. Facultad de Ciencias. Sección Genética Evolutiva. Montevideo, Uruguay.; LAURA BENTANCOR, Universidad de la República Oriental del Uruguay. Facultad de Medicina. Instituto de Higiene. Departamento de Bacteriología y Virología. Montevideo, Uruguay.; MARTIN FRAGA COTELO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JORGE GIL, Universidad de la República Oriental del Uruguay. Facultad de Veterinaria. Estación Experimental "Dr. Mario A. Cassinoni". Departamento de Salud de los Sistemas Pecuarios. Paysandú, Uruguay.; GREGORIO IRAOLA, Institut Pasteur de Montevideo. Laboratorio de Genómica Microbiana, Montevideo, Uruguay. / Universidad Mayor. Facultad de Ciencias. Centro de Biología Integrativa. Santiago de Chile, Chile.; CLAUDIA MORSELLA, Estación Experimental INTA Balcarce. Laboratorio de Bacteriología. Balcarce, Buenos Aires, Argentina.; FERNANDO PAOLICCHI, Estación Experimental INTA Balcarce. Laboratorio de Bacteriología. Balcarce, Buenos Aires, Argentina.; RUBEN PEREZ, Universidad de la República Oriental del Uruguay. Facultad de Ciencias. Sección Genética Evolutiva. Montevideo, Uruguay.; FRANKLIN RIET-CORREA AMARAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; Universidad de la República Oriental del Uruguay. Facultad de Ciencias. Sección Genética Evolutiva. Montevideo, Uruguay.; ALFONSO SILVA, Universidad de la República Oriental del Uruguay. Facultad de Ciencias. Sección Genética Evolutiva. Montevideo, Uruguay.; CAROLINE DA SILVA SILVEIRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; LUCÍA CALLEROS, Universidad de la República Oriental del Uruguay. Facultad de Ciencias. Sección Genética Evolutiva. Montevideo, Uruguay. |
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Título : |
Accurate and fast identification of Campylobacter fetus in bulls by real-time PCR targeting a 16S rRNA gene sequence. |
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Fecha de publicación : |
2021 |
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Fuente / Imprenta : |
Veterinary and Animal Science, January 2021, vol.11 no. 100165, 5 p. OPEN ACCESS. Doi: https://doi.org/10.1016/j.vas.2020.100163 |
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DOI : |
10.1016/j.vas.2020.100163 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 21 October 2020 / Received in revised form 20 December 2020 / Accepted 22 December 2020 / available online 24 December 2020.
Corresponding author: laurabet@higiene.edu.uy |
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Contenido : |
Campylobacter fetus is an important animal pathogen that causes infectious infertility, embryonic mortality and abortions in cattle and sheep flocks. There are two recognized subspecies related with reproductive disorders in livestock: Campylobacter fetus subsp. fetus (Cff) and Campylobacter fetus subsp. venerealis (Cfv). Rapid and reliable detection of this pathogenic species in bulls is of upmost importance for disease control in dairy and beef herds as they are asymptomatic carriers. The aim of the present work was to assess the performance a real-time PCR (qPCR) method for the diagnosis of Campylobacter fetus in samples from bulls, comparing it with culture and isolation methods. 520 preputial samples were both cultured in Skirrow?s medium and analyzed by qPCR. The estimated sensitivity of qPCR was 90.9% (95% CI, 69.4%?100%), and the specificity was 99.4% (95% CI, 98.6% - 100%). The proportion of C. fetus positive individuals was 2.1% by isolation and 2.5% by qPCR. Isolates were identified by biochemical tests as Cfv (n = 9) and Cff (n = 2). Our findings support the use of qPCR for fast and accurate detection of C. fetus directly from field samples of preputial smegma of bulls. The qPCR method showed to be suitable for massive screenings because it can be performed in pooled samples without losing accuracy and sensitivity. |
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Palabras claves : |
BOVINE GENITAL CAMPYLOBACTERIOSIS; CAMPYLOBACTER FETUS; MOLECULAR DIAGNOSIS; MOLECULAR DIAGNOSTICS; QPCR. |
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Asunto categoría : |
L73 Enfermedades de los animales |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/14934/1/Veterinary-Animal-Science-2021-100163.pdf
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Marc : |
LEADER 02681naa a2200373 a 4500
001 1061678
005 2021-04-14
008 2021 bl uuuu u00u1 u #d
024 7 $a10.1016/j.vas.2020.100163$2DOI
100 1 $aDELPIAZZO, R.
245 $aAccurate and fast identification of Campylobacter fetus in bulls by real-time PCR targeting a 16S rRNA gene sequence.$h[electronic resource]
260 $c2021
500 $aArticle history: Received 21 October 2020 / Received in revised form 20 December 2020 / Accepted 22 December 2020 / available online 24 December 2020. Corresponding author: laurabet@higiene.edu.uy
520 $aCampylobacter fetus is an important animal pathogen that causes infectious infertility, embryonic mortality and abortions in cattle and sheep flocks. There are two recognized subspecies related with reproductive disorders in livestock: Campylobacter fetus subsp. fetus (Cff) and Campylobacter fetus subsp. venerealis (Cfv). Rapid and reliable detection of this pathogenic species in bulls is of upmost importance for disease control in dairy and beef herds as they are asymptomatic carriers. The aim of the present work was to assess the performance a real-time PCR (qPCR) method for the diagnosis of Campylobacter fetus in samples from bulls, comparing it with culture and isolation methods. 520 preputial samples were both cultured in Skirrow?s medium and analyzed by qPCR. The estimated sensitivity of qPCR was 90.9% (95% CI, 69.4%?100%), and the specificity was 99.4% (95% CI, 98.6% - 100%). The proportion of C. fetus positive individuals was 2.1% by isolation and 2.5% by qPCR. Isolates were identified by biochemical tests as Cfv (n = 9) and Cff (n = 2). Our findings support the use of qPCR for fast and accurate detection of C. fetus directly from field samples of preputial smegma of bulls. The qPCR method showed to be suitable for massive screenings because it can be performed in pooled samples without losing accuracy and sensitivity.
653 $aBOVINE GENITAL CAMPYLOBACTERIOSIS
653 $aCAMPYLOBACTER FETUS
653 $aMOLECULAR DIAGNOSIS
653 $aMOLECULAR DIAGNOSTICS
653 $aQPCR
700 1 $aBARCELLOS, M.
700 1 $aBARROS, S.
700 1 $aBENTANCOR, L.
700 1 $aFRAGA, M.
700 1 $aGIL, J.
700 1 $aIRAOLA, G.
700 1 $aMORSELLA, C.
700 1 $aPAOLICCHI, F.
700 1 $aPÉREZ, R.
700 1 $aRIET-CORREA, F.
700 1 $aSANGUINETTI, M.
700 1 $aSILVA, A.
700 1 $aSILVEIRA, C.S.
700 1 $aCALLEROS, L.
773 $tVeterinary and Animal Science, January 2021, vol.11 no. 100165, 5 p. OPEN ACCESS. Doi: https://doi.org/10.1016/j.vas.2020.100163
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INIA Treinta y Tres (TT) |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
04/03/2024 |
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Actualizado : |
04/03/2024 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Nacionales |
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Circulación / Nivel : |
Nacional - -- |
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Autor : |
OTERO, A.; GOÑI, C.; GRASSO, R.; MACHADO, D.; RIVAS, F. |
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Afiliación : |
ALVARO RICARDO OTERO CAMA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CARMEN TERESITA GOÑI ALTUNA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RAFAEL GILLSON GRASSO RODRIGUEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; DELIA ROXANA MACHADO TEXEIRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CARLOS FERNANDO RIVAS GRELA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Water productivity associated to the planting system in young Nadorcott mandarin plants. [Productividad del agua asociada al sistema de plantación en plantas jóvenes de mandarina Nadorcott.]. [Produtividade hídrica em relação ao sistema de plantio em plantas jovens de tangerineira Nadorcott.]. |
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Complemento del título : |
Advances in Water in Agroscience. Irrigation and water management. |
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Fecha de publicación : |
2023 |
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Fuente / Imprenta : |
Agrociencia Uruguay, 2023, Vol.27(NE1), e1188. https://doi.org/10.31285/AGRO.27.1188 -- OPEN ACCESS. |
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ISSN : |
2730-5066 |
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DOI : |
10.31285/AGRO.27.1188 |
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Idioma : |
Español |
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Notas : |
Article history: Received 29 April 2023; Accepted 23 August 2023; Published 06 February 2024. -- Editor: Lucía Puppo, Universidad de la República, Montevideo, Uruguay. -- Correspondence: Álvaro Otero, aotero@inia.org.uy -- License: This work is licensed under a Creative Commons Attribution 4.0 International License. (https://creativecommons.org/licenses/by/4.0/ ) |
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Contenido : |
ABSTRACT.- The main goal of citriculture in Uruguay is to increase yield, quality, and improve sustainability and efficiency of natural resources. Today, it is not possible to conceive an efficient citrus production system without the use of irrigation technologies. The aim of this work was to evaluate the irrigation water efficiency and growth conditions of different plantation systems focused on speeding up production and the improvement of water use efficiency. An experiment was installed in Nadorcott mandarin with two planting densities (909 and 1220 pl. ha-1), and three plantation designs: ridges covered with white plastic cloth; uncovered ridges, and flat planting at ground level. At the end of the evaluation period, the volume of the trees planted in the covered ridges doubled those in the flat (1 m3). The trees on the flat design consistently produced fewer fruits and lower yield than those on ridges. Covered ridge showed the best performance. Soil water content remained higher and soil temperature lower in covered ridges. Overall, trees planted on ridges with or without plastic mulch allowed a better water use efficiency, and the lower soil temperature promoted a better environment for tree growth. .-.-.-.-.-.-.-.-.-.- RESUMEN.- El principal objetivo de la citricultura en Uruguay es incrementar el rendimiento, la calidad, mejorar la sostenibilidad y la eficiencia de los recursos naturales. Actualmente no es posible concebir un sistema eficiente de producción de cítricos sin el uso de tecnologías de riego. El objetivo de este trabajo fue incorporar el concepto de productividad del agua al comparar diferentes sistemas de plantación enfocados en acelerar la producción. Para ello se instaló un experimento en mandarina Nadorcott con dos densidades de plantación (909 y 1220 pl. ha-1) y tres diseños de plantación: camellones cubiertos con tela plástica blanca; camellones descubiertos y plantación plana a nivel del suelo. Al final del período de evaluación, el volumen de los árboles plantados sobre los camellones cubiertos duplicó al de los plantados en plano (1 m3). Los árboles en el sistema en plano consistentemente produjeron menos frutos y con menor rendimiento que las plantas sobre los camellones. El diseño de plantación en camellón cubierto mostró el mejor desempeño. El contenido de agua del suelo se mantuvo más alto y la temperatura del suelo más baja en los camellones cubiertos. En general, los árboles plantados en camellones con o sin cubierta plástica permitieron una mejor productividad en el uso del agua y la temperatura más baja del suelo, creando mejores condiciones de crecimiento del árbol. .-.-.-.-.-.-.-.-.-.- RESUMO.- A citricultura no Uruguai tem como objetivo imediato aumentar o rendimento, a qualidade, melhorando a sustentabilidade e a eficiência dos recursos naturais. Atualmente não é possível conceber um sistema eficiente de produção de citros sem o uso de tecnologias de irrigação. O objetivo deste trabalho foi incorporar o conceito de produtividade hídrica na comparação de diferentes sistemas de plantio com foco na aceleração da produção. Para isso, foi instalado um experimento em tangerineira Nadorcott com duas densidades de plantio (909 e 1220 pl. ha-1) e três desenhos de plantio: ca-malhões cobertos com lona plástica branca; camalhões descobertos e plantação plana ao nível do solo. Ao final do período de avaliação, o volume de árvores plantadas em camalhões cobertas dobrou em relação às plantadas planas (1 m3). As árvores no sistema plano produziram consistentemente menos frutos e rendimentos mais baixos do que as plantas nos cumes. O delineamento de plantio em camalhões cobertos apresentou o melhor desempenho. O teor de água no solo permanece u mais alto e a temperatura do solo mais baixa nas camalhões cobertas. Em geral, as árvores plantadas em camalhões com ou sem cobertura plástica permitiram maior produtividade no uso da água e menor tem-peratura do solo, criando melhores condições para o crescimento das árvores. @2023 Agrociencia Uruguay. MenosABSTRACT.- The main goal of citriculture in Uruguay is to increase yield, quality, and improve sustainability and efficiency of natural resources. Today, it is not possible to conceive an efficient citrus production system without the use of irrigation technologies. The aim of this work was to evaluate the irrigation water efficiency and growth conditions of different plantation systems focused on speeding up production and the improvement of water use efficiency. An experiment was installed in Nadorcott mandarin with two planting densities (909 and 1220 pl. ha-1), and three plantation designs: ridges covered with white plastic cloth; uncovered ridges, and flat planting at ground level. At the end of the evaluation period, the volume of the trees planted in the covered ridges doubled those in the flat (1 m3). The trees on the flat design consistently produced fewer fruits and lower yield than those on ridges. Covered ridge showed the best performance. Soil water content remained higher and soil temperature lower in covered ridges. Overall, trees planted on ridges with or without plastic mulch allowed a better water use efficiency, and the lower soil temperature promoted a better environment for tree growth. .-.-.-.-.-.-.-.-.-.- RESUMEN.- El principal objetivo de la citricultura en Uruguay es incrementar el rendimiento, la calidad, mejorar la sostenibilidad y la eficiencia de los recursos naturales. Actualmente no es posible concebir un sistema eficiente de producción de cítr... Presentar Todo |
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Palabras claves : |
Mulching; Planting system; Productividad del agua; Produtividade hídrica; Sistema de plantación; Sistema de plantio; Water productivity. |
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Thesagro : |
CÍTRICOS; CITRUS. |
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Asunto categoría : |
P10 Recursos hídricos y su ordenación |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/17512/1/2730-5066-1188.pdf
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Marc : |
LEADER 05608naa a2200313 a 4500
001 1064488
005 2024-03-04
008 2023 bl uuuu u00u1 u #d
022 $a2730-5066
024 7 $a10.31285/AGRO.27.1188$2DOI
100 1 $aOTERO, A.
245 $aWater productivity associated to the planting system in young Nadorcott mandarin plants. [Productividad del agua asociada al sistema de plantación en plantas jóvenes de mandarina Nadorcott.]. [Produtividade hídrica em relação ao sistema de plantio em plantas jovens de tangerineira Nadorcott.].$h[electronic resource]
260 $c2023
500 $aArticle history: Received 29 April 2023; Accepted 23 August 2023; Published 06 February 2024. -- Editor: Lucía Puppo, Universidad de la República, Montevideo, Uruguay. -- Correspondence: Álvaro Otero, aotero@inia.org.uy -- License: This work is licensed under a Creative Commons Attribution 4.0 International License. (https://creativecommons.org/licenses/by/4.0/ )
520 $aABSTRACT.- The main goal of citriculture in Uruguay is to increase yield, quality, and improve sustainability and efficiency of natural resources. Today, it is not possible to conceive an efficient citrus production system without the use of irrigation technologies. The aim of this work was to evaluate the irrigation water efficiency and growth conditions of different plantation systems focused on speeding up production and the improvement of water use efficiency. An experiment was installed in Nadorcott mandarin with two planting densities (909 and 1220 pl. ha-1), and three plantation designs: ridges covered with white plastic cloth; uncovered ridges, and flat planting at ground level. At the end of the evaluation period, the volume of the trees planted in the covered ridges doubled those in the flat (1 m3). The trees on the flat design consistently produced fewer fruits and lower yield than those on ridges. Covered ridge showed the best performance. Soil water content remained higher and soil temperature lower in covered ridges. Overall, trees planted on ridges with or without plastic mulch allowed a better water use efficiency, and the lower soil temperature promoted a better environment for tree growth. .-.-.-.-.-.-.-.-.-.- RESUMEN.- El principal objetivo de la citricultura en Uruguay es incrementar el rendimiento, la calidad, mejorar la sostenibilidad y la eficiencia de los recursos naturales. Actualmente no es posible concebir un sistema eficiente de producción de cítricos sin el uso de tecnologías de riego. El objetivo de este trabajo fue incorporar el concepto de productividad del agua al comparar diferentes sistemas de plantación enfocados en acelerar la producción. Para ello se instaló un experimento en mandarina Nadorcott con dos densidades de plantación (909 y 1220 pl. ha-1) y tres diseños de plantación: camellones cubiertos con tela plástica blanca; camellones descubiertos y plantación plana a nivel del suelo. Al final del período de evaluación, el volumen de los árboles plantados sobre los camellones cubiertos duplicó al de los plantados en plano (1 m3). Los árboles en el sistema en plano consistentemente produjeron menos frutos y con menor rendimiento que las plantas sobre los camellones. El diseño de plantación en camellón cubierto mostró el mejor desempeño. El contenido de agua del suelo se mantuvo más alto y la temperatura del suelo más baja en los camellones cubiertos. En general, los árboles plantados en camellones con o sin cubierta plástica permitieron una mejor productividad en el uso del agua y la temperatura más baja del suelo, creando mejores condiciones de crecimiento del árbol. .-.-.-.-.-.-.-.-.-.- RESUMO.- A citricultura no Uruguai tem como objetivo imediato aumentar o rendimento, a qualidade, melhorando a sustentabilidade e a eficiência dos recursos naturais. Atualmente não é possível conceber um sistema eficiente de produção de citros sem o uso de tecnologias de irrigação. O objetivo deste trabalho foi incorporar o conceito de produtividade hídrica na comparação de diferentes sistemas de plantio com foco na aceleração da produção. Para isso, foi instalado um experimento em tangerineira Nadorcott com duas densidades de plantio (909 e 1220 pl. ha-1) e três desenhos de plantio: ca-malhões cobertos com lona plástica branca; camalhões descobertos e plantação plana ao nível do solo. Ao final do período de avaliação, o volume de árvores plantadas em camalhões cobertas dobrou em relação às plantadas planas (1 m3). As árvores no sistema plano produziram consistentemente menos frutos e rendimentos mais baixos do que as plantas nos cumes. O delineamento de plantio em camalhões cobertos apresentou o melhor desempenho. O teor de água no solo permanece u mais alto e a temperatura do solo mais baixa nas camalhões cobertas. Em geral, as árvores plantadas em camalhões com ou sem cobertura plástica permitiram maior produtividade no uso da água e menor tem-peratura do solo, criando melhores condições para o crescimento das árvores. @2023 Agrociencia Uruguay.
650 $aCÍTRICOS
650 $aCITRUS
653 $aMulching
653 $aPlanting system
653 $aProductividad del agua
653 $aProdutividade hídrica
653 $aSistema de plantación
653 $aSistema de plantio
653 $aWater productivity
700 1 $aGOÑI, C.
700 1 $aGRASSO, R.
700 1 $aMACHADO, D.
700 1 $aRIVAS, F.
773 $tAgrociencia Uruguay, 2023, Vol.27(NE1), e1188. https://doi.org/10.31285/AGRO.27.1188 -- OPEN ACCESS.
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