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Registro completo
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Biblioteca (s) : |
INIA Treinta y Tres. |
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Fecha : |
05/04/2022 |
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Actualizado : |
01/09/2022 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
SCHVARTZMAN, C.; FRESIA, P.; MURCHIO, S.; MUJICA, V.; DALLA RIZZA, M. |
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Afiliación : |
CLAUDIA SCHVARTZMAN DISEGNI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; PABLO FRESIA, Unidad Mixta UMPI, Institut Pasteur Montevideo + INIA, Montevideo, Uruguay; MARIA SARA MURCHIO VIGNOLO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARIA VALENTINA MUJICA TELIZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARCO DALLA RIZZA VILARO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
RNAi in Piezodorus guildinii (Hemiptera: Pentatomidae): transcriptome assembly for the development of pest control strategies. |
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Fecha de publicación : |
2022 |
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Fuente / Imprenta : |
Frontiers in Plant Science, 01 April 2022, volume13, article 804839. OPEN ACCESS. Doi: https://doi.org/10.3389/fpls.2022.804839 |
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Páginas : |
13 p. |
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DOI : |
10.3389/fpls.2022.804839 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: 29 October 2021; Accepted: 28 February 2022; Published: 01 April 2022.
Correspondence: Marco Dalla-Rizza, mdallarizza@inia.org.uy
Al abrir el registro se visualiza el enlace al material suplementario. |
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Contenido : |
Red-banded stink bug Piezodorus guildinii (P. guildinii) has been described as the most damaging stink bug regarding soybean crops, leading to seed injury, low germination percentages, and foliar retention, at low population densities. In recent years, RNA interference (RNAi), a conserved eukaryote silencing mechanism has been explored to develop species-selective pesticides. In this work, we evaluated RNAi in P. guildinii to develop new pest-control strategies. For this, we assembled and annotated a P. guildinii transcriptome from a pool of all developmental stages. Analysis of this transcriptome led to the identification of 56 genes related to the silencing process encompassing siRNA, miRNA, and piRNA pathways. To evaluate the functionality of RNAi machinery, P. guildinii adults were injected with 28 ng/mg of body weight of double stranded RNA (dsRNA) targeting vATPase A. A mortality of 35 and 51.6% was observed after 7 and 14 days, respectively, and a downregulation of vATPase A gene of 84% 72 h post-injection. In addition, Dicer-2 and Argonaute-2 genes, core RNAi proteins, were upregulated 1.8-fold 48 h after injection. These findings showed for the first time that RNAi is functional in P. guildinii and the silencing of essential genes has a significant effect in adult viability. Taken together, the work reported here shows that RNAi could be an interesting approach for the development of red-banded stink bug control strategies. |
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Palabras claves : |
DSRNA,; PEST CONTROL; RNA-SEQ; RNAI; STINK BUG; VATPASE A,. |
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Asunto categoría : |
H10 Plagas de las plantas |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/16649/1/Frontiers-Plant-Science-2022-Schvartzman.pdf
http://www.ainfo.inia.uy/digital/bitstream/item/16650/1/Frontiers-Plant-Science-2022-Schvartzman-1.PDF
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Marc : |
LEADER 02536naa a2200277 a 4500
001 1062960
005 2022-09-01
008 2022 bl uuuu u00u1 u #d
024 7 $a10.3389/fpls.2022.804839$2DOI
100 1 $aSCHVARTZMAN, C.
245 $aRNAi in Piezodorus guildinii (Hemiptera$bPentatomidae): transcriptome assembly for the development of pest control strategies.$h[electronic resource]
260 $c2022
300 $a13 p.
500 $aArticle history: Received: 29 October 2021; Accepted: 28 February 2022; Published: 01 April 2022. Correspondence: Marco Dalla-Rizza, mdallarizza@inia.org.uy Al abrir el registro se visualiza el enlace al material suplementario.
520 $aRed-banded stink bug Piezodorus guildinii (P. guildinii) has been described as the most damaging stink bug regarding soybean crops, leading to seed injury, low germination percentages, and foliar retention, at low population densities. In recent years, RNA interference (RNAi), a conserved eukaryote silencing mechanism has been explored to develop species-selective pesticides. In this work, we evaluated RNAi in P. guildinii to develop new pest-control strategies. For this, we assembled and annotated a P. guildinii transcriptome from a pool of all developmental stages. Analysis of this transcriptome led to the identification of 56 genes related to the silencing process encompassing siRNA, miRNA, and piRNA pathways. To evaluate the functionality of RNAi machinery, P. guildinii adults were injected with 28 ng/mg of body weight of double stranded RNA (dsRNA) targeting vATPase A. A mortality of 35 and 51.6% was observed after 7 and 14 days, respectively, and a downregulation of vATPase A gene of 84% 72 h post-injection. In addition, Dicer-2 and Argonaute-2 genes, core RNAi proteins, were upregulated 1.8-fold 48 h after injection. These findings showed for the first time that RNAi is functional in P. guildinii and the silencing of essential genes has a significant effect in adult viability. Taken together, the work reported here shows that RNAi could be an interesting approach for the development of red-banded stink bug control strategies.
653 $aDSRNA,
653 $aPEST CONTROL
653 $aRNA-SEQ
653 $aRNAI
653 $aSTINK BUG
653 $aVATPASE A,
700 1 $aFRESIA, P.
700 1 $aMURCHIO, S.
700 1 $aMUJICA, V.
700 1 $aDALLA RIZZA, M.
773 $tFrontiers in Plant Science, 01 April 2022, volume13, article 804839. OPEN ACCESS. Doi: https://doi.org/10.3389/fpls.2022.804839
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INIA Treinta y Tres (TT) |
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Registro completo
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Biblioteca (s) : |
INIA Tacuarembó. |
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Fecha actual : |
31/03/2021 |
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Actualizado : |
04/05/2021 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
HIRIGOYEN, A.; NAVARRO-CERRILLO, R.; BAGNARA, M.; FRANCO, J.; RESQUÍN, F.; RACHID, C. |
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Afiliación : |
ANDRES EDUARDO HIRIGOYEN DOMINGUEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RAFAEL NAVARRO-CERRILLO, Department of Forestry Engineering, Laboratory of Silviculture, Dendrochronology and Climate Change, DendrodatLab- ERSAF, University of Cordoba, Córdoba; MAURIZIO BAGNARA, Senckenberg Biodiversity and Climate Research Centre (SBiK-F), Frankfurt am Main (Germany); JORGE FRANCO, University of the Republic, Faculty of Agronomy, Paysandú (Uruguay); JOSE FERNANDO RESQUIN PEREZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ANA CECILIA RACHID CASNATI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Modelling taper and stem volume considering stand density in Eucalyptus grandis and Eucalyptus dunnii. |
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Fecha de publicación : |
2021 |
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Fuente / Imprenta : |
i Forest - Biogeosciences and Forestry, 2021, Volume 14, Issue 2, Pages 127-136.OPEN ACCESS. DOI: https://doi.org/10.3832/ifor3604-014 |
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DOI : |
10.3832/ifor3604-014 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: Jul 31, 2020 - Accepted: Jan 15, 2021. Acknowledgments: The authors thank the Instituto Nacional de Investigaciones Agropecuarias (INIAUruguay) for supporting fieldwork and the INIA Scholarship for PhD studies. We are particularly grateful for the support of Leonidas Carrasco Letelier, Roberto Scoz, Demian Gomez, Gonzalo Martinez, Jorge Basso (INIA) and Juan Gabriel Álvarez González (USC). We acknowledge the institutional support of the University of Cordoba ? Campus de Excelencia CEIA3. We also thank the ERSAF group. We thank Dr. David Walker for his revisions of the manuscript versions, and the anonymous referees for their comments and corrections. |
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Contenido : |
Eucalyptus grandis and Eucalyptus dunnii are the most planted tree species in Uruguay. Anticipating information about the quantity and quality of wood is important for managing intensive forest plantation. The estimate of merchantable and total wood volume is an essential tool in forest planning and management. The aim of this study was to evaluate four systems of taper and merchantable volume that consisted in a taper, a merchantable volume and a total tree volume function. A modified second-order continuous autoregressive error structure corrected the inherent serial autocorrelation of different observations in one tree. Taper and volume equations were fitted simultaneously after autocorrelation correction by full information maximum likelihood method. The segmented system proposed by Fang et al. (2000) produced the best fit as it explained more than 98% of the taper, merchantable volume and total volume variability for both species. In addition, precision of the segmented system was compared with and without incorporating stand density as a variable. Results of this analysis showed that for E. grandis, the predictive accuracy of the model was improved by including the stand density variable, whereas for E. dunnii this variable was not statistically significant. This modelling framework provides an improvement in taper and tree volume predictions for E. dunnii and E. grandis in Uruguay. The possibilities offered by this methodology could be of interest for its application in countries where fast growing plantations are managed. MenosEucalyptus grandis and Eucalyptus dunnii are the most planted tree species in Uruguay. Anticipating information about the quantity and quality of wood is important for managing intensive forest plantation. The estimate of merchantable and total wood volume is an essential tool in forest planning and management. The aim of this study was to evaluate four systems of taper and merchantable volume that consisted in a taper, a merchantable volume and a total tree volume function. A modified second-order continuous autoregressive error structure corrected the inherent serial autocorrelation of different observations in one tree. Taper and volume equations were fitted simultaneously after autocorrelation correction by full information maximum likelihood method. The segmented system proposed by Fang et al. (2000) produced the best fit as it explained more than 98% of the taper, merchantable volume and total volume variability for both species. In addition, precision of the segmented system was compared with and without incorporating stand density as a variable. Results of this analysis showed that for E. grandis, the predictive accuracy of the model was improved by including the stand density variable, whereas for E. dunnii this variable was not statistically significant. This modelling framework provides an improvement in taper and tree volume predictions for E. dunnii and E. grandis in Uruguay. The possibilities offered by this methodology could be of interest for its application ... Presentar Todo |
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Palabras claves : |
COMPATIBLE SYSTEMS; EUCALYPTUS; FOREST AND FORESTRY; INTENSIVE SILVICULTURE; SIMULTANEOUS ESTIMATION; TAPER. |
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Thesagro : |
FORESTACIÓN. |
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Asunto categoría : |
K10 Producción forestal |
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URL : |
https://iforest.sisef.org/pdf/?id=ifor3604-014
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Marc : |
LEADER 03161naa a2200289 a 4500
001 1061913
005 2021-05-04
008 2021 bl uuuu u00u1 u #d
024 7 $a10.3832/ifor3604-014$2DOI
100 1 $aHIRIGOYEN, A.
245 $aModelling taper and stem volume considering stand density in Eucalyptus grandis and Eucalyptus dunnii.$h[electronic resource]
260 $c2021
500 $aArticle history: Received: Jul 31, 2020 - Accepted: Jan 15, 2021. Acknowledgments: The authors thank the Instituto Nacional de Investigaciones Agropecuarias (INIAUruguay) for supporting fieldwork and the INIA Scholarship for PhD studies. We are particularly grateful for the support of Leonidas Carrasco Letelier, Roberto Scoz, Demian Gomez, Gonzalo Martinez, Jorge Basso (INIA) and Juan Gabriel Álvarez González (USC). We acknowledge the institutional support of the University of Cordoba ? Campus de Excelencia CEIA3. We also thank the ERSAF group. We thank Dr. David Walker for his revisions of the manuscript versions, and the anonymous referees for their comments and corrections.
520 $aEucalyptus grandis and Eucalyptus dunnii are the most planted tree species in Uruguay. Anticipating information about the quantity and quality of wood is important for managing intensive forest plantation. The estimate of merchantable and total wood volume is an essential tool in forest planning and management. The aim of this study was to evaluate four systems of taper and merchantable volume that consisted in a taper, a merchantable volume and a total tree volume function. A modified second-order continuous autoregressive error structure corrected the inherent serial autocorrelation of different observations in one tree. Taper and volume equations were fitted simultaneously after autocorrelation correction by full information maximum likelihood method. The segmented system proposed by Fang et al. (2000) produced the best fit as it explained more than 98% of the taper, merchantable volume and total volume variability for both species. In addition, precision of the segmented system was compared with and without incorporating stand density as a variable. Results of this analysis showed that for E. grandis, the predictive accuracy of the model was improved by including the stand density variable, whereas for E. dunnii this variable was not statistically significant. This modelling framework provides an improvement in taper and tree volume predictions for E. dunnii and E. grandis in Uruguay. The possibilities offered by this methodology could be of interest for its application in countries where fast growing plantations are managed.
650 $aFORESTACIÓN
653 $aCOMPATIBLE SYSTEMS
653 $aEUCALYPTUS
653 $aFOREST AND FORESTRY
653 $aINTENSIVE SILVICULTURE
653 $aSIMULTANEOUS ESTIMATION
653 $aTAPER
700 1 $aNAVARRO-CERRILLO, R.
700 1 $aBAGNARA, M.
700 1 $aFRANCO, J.
700 1 $aRESQUÍN, F.
700 1 $aRACHID, C.
773 $ti Forest - Biogeosciences and Forestry, 2021, Volume 14, Issue 2, Pages 127-136.OPEN ACCESS. DOI: https://doi.org/10.3832/ifor3604-014
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