|
|
| Acceso al texto completo restringido a Biblioteca INIA Treinta y Tres. Por información adicional contacte bibliott@inia.org.uy. |
Registro completo
|
Biblioteca (s) : |
INIA Treinta y Tres. |
Fecha : |
03/12/2020 |
Actualizado : |
03/12/2020 |
Autor : |
NARANCIO, R.; DING, Y.-L.; LIN, Y.-H.; SAHAB, S.; PANTER, S.; HAYES, M.; JOHN, U.; ANDERSON, H.; MASON, J.; SPANGENBERG, G. |
Afiliación : |
RAFAEL NARANCIO FERES, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; Agriculture Victoria Research, AgriBio, Centre for AgriBioscience, Bundoora, Australia; School of Applied Systems Biology, La Trobe University, Bundoora, Australia; YONG?LIN DING, Agriculture Victoria Research, AgriBio, Centre for AgriBioscience, Bundoora, Australia; YI?HAN LIN, Agriculture Victoria Research, AgriBio, Centre for AgriBioscience, Bundoora, Australia; SAREENA SAHAB, Agriculture Victoria Research, AgriBio, Centre for AgriBioscience, Bundoora, Australia; STEPHEN PANTER, Agriculture Victoria Research, AgriBio, Centre for AgriBioscience, Bundoora, Australia; MATTHEW HAYES, Triffid BioScience, PO Box 1986, Carlton South, Australia; ULRIK JOHN, Agriculture Victoria Research, AgriBio, Centre for AgriBioscience, Bundoora, Australia; HEATHER ANDERSON, Agriculture Victoria Research, AgriBio, Centre for AgriBioscience, Bundoora, Australia; JOHN MASON, Agriculture Victoria Research, AgriBio, Centre for AgriBioscience, Bundoora, Australia; School of Applied Systems Biology, La Trobe University, Bundoora, Australia; GERMAN SPANGENBERG, Agriculture Victoria Research, AgriBio, Centre for AgriBioscience, Bundoora, Australia; School of Applied Systems Biology, La Trobe University, Bundoora, Australia. |
Título : |
Correction to: Application of linked and unlinked co‑transformation to generate triple stack, marker‑free, transgenic white clover (Trifolium repens L.) (Plant Cell, Tissue and Organ Culture (PCTOC), (2020), 142, 3, (635-646), 10.1007/s11240-020-01891-6). |
Fecha de publicación : |
2020 |
Fuente / Imprenta : |
Plant Cell, Tissue and Organ Culture, 2020. DOI: https://doi.org/10.1007/s11240-020-01891-6 |
DOI : |
10.1007/s11240-020-01891-6 |
Idioma : |
Inglés |
Notas : |
Article history: Received: 19 May 2020 / Accepted: 15 July 2020. |
Contenido : |
ABSTRACT.
Both linked and un-linked co-transformation can be used to overcome limitations of methods, such as re-transformation or sexual crossing of transgenic plants, to enable transfer of multiple genes to a single plant. Un-linked co-transformation can also facilitate the production of selectable marker-free transgenic plants. In this study, transgenic white clover plants were generated by Agrobacterium-mediated linked co-transformation using a single T-DNA of 9803 bp expressing: an isopentenyl transferase (IPT) gene for delayed leaf senescence under the control of an organ specific MYB32 promoter from Arabidopsis, a white clover nodule enhanced malate dehydrogenase (neMDH) gene for aluminium tolerance controlled by the endogenous Phosphate Transporter 1 (PT1) promoter, and the coat protein gene from Alfalfa Mosaic Virus (CP-AMV) controlled by the 35S promoter from Cauliflower Mosaic Virus. The selectable marker gene encoding hygromycin phosphotransferase (hph) was borne on a separate T-DNA. Forty independent transgenic events carrying the triple stack were generated, with estimated co-transformation efficiencies of 0.22 to 0.23%. Forty three percent of the events generated had a single insertion, while two events were selectable marker-free. Transcript abundance studies of the three transgenes of interest demonstrated the transcriptional competence of the inserted T-DNA. This study illustrates the feasibility of transferring multiple genes in a large single T-DNA into white clover by Agrobacterium-mediated co-transformation. Furthermore, observations of consistently delayed leaf senescence, statistically significant increases in TrneMDH transcript, and presence of CP-AMV transcript, support further analysis of these events for delayed leaf senescence under drought conditions, aluminium tolerance, and resistance to AMV.
© 2020, Springer Nature B.V. MenosABSTRACT.
Both linked and un-linked co-transformation can be used to overcome limitations of methods, such as re-transformation or sexual crossing of transgenic plants, to enable transfer of multiple genes to a single plant. Un-linked co-transformation can also facilitate the production of selectable marker-free transgenic plants. In this study, transgenic white clover plants were generated by Agrobacterium-mediated linked co-transformation using a single T-DNA of 9803 bp expressing: an isopentenyl transferase (IPT) gene for delayed leaf senescence under the control of an organ specific MYB32 promoter from Arabidopsis, a white clover nodule enhanced malate dehydrogenase (neMDH) gene for aluminium tolerance controlled by the endogenous Phosphate Transporter 1 (PT1) promoter, and the coat protein gene from Alfalfa Mosaic Virus (CP-AMV) controlled by the 35S promoter from Cauliflower Mosaic Virus. The selectable marker gene encoding hygromycin phosphotransferase (hph) was borne on a separate T-DNA. Forty independent transgenic events carrying the triple stack were generated, with estimated co-transformation efficiencies of 0.22 to 0.23%. Forty three percent of the events generated had a single insertion, while two events were selectable marker-free. Transcript abundance studies of the three transgenes of interest demonstrated the transcriptional competence of the inserted T-DNA. This study illustrates the feasibility of transferring multiple genes in a large single T-DNA into w... Presentar Todo |
Palabras claves : |
Agrobacterium-mediated transformation; Delayed leaf senescence; Multigene; T-DNA. |
Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
Marc : |
LEADER 03007naa a2200301 a 4500 001 1061539 005 2020-12-03 008 2020 bl uuuu u00u1 u #d 024 7 $a10.1007/s11240-020-01891-6$2DOI 100 1 $aNARANCIO, R. 245 $aCorrection to$bApplication of linked and unlinked co‑transformation to generate triple stack, marker‑free, transgenic white clover (Trifolium repens L.) (Plant Cell, Tissue and Organ Culture (PCTOC), (2020), 142, 3, (635-646), 10.1007/s11240-020-01891-6).$h[electronic resource] 260 $c2020 500 $aArticle history: Received: 19 May 2020 / Accepted: 15 July 2020. 520 $aABSTRACT. Both linked and un-linked co-transformation can be used to overcome limitations of methods, such as re-transformation or sexual crossing of transgenic plants, to enable transfer of multiple genes to a single plant. Un-linked co-transformation can also facilitate the production of selectable marker-free transgenic plants. In this study, transgenic white clover plants were generated by Agrobacterium-mediated linked co-transformation using a single T-DNA of 9803 bp expressing: an isopentenyl transferase (IPT) gene for delayed leaf senescence under the control of an organ specific MYB32 promoter from Arabidopsis, a white clover nodule enhanced malate dehydrogenase (neMDH) gene for aluminium tolerance controlled by the endogenous Phosphate Transporter 1 (PT1) promoter, and the coat protein gene from Alfalfa Mosaic Virus (CP-AMV) controlled by the 35S promoter from Cauliflower Mosaic Virus. The selectable marker gene encoding hygromycin phosphotransferase (hph) was borne on a separate T-DNA. Forty independent transgenic events carrying the triple stack were generated, with estimated co-transformation efficiencies of 0.22 to 0.23%. Forty three percent of the events generated had a single insertion, while two events were selectable marker-free. Transcript abundance studies of the three transgenes of interest demonstrated the transcriptional competence of the inserted T-DNA. This study illustrates the feasibility of transferring multiple genes in a large single T-DNA into white clover by Agrobacterium-mediated co-transformation. Furthermore, observations of consistently delayed leaf senescence, statistically significant increases in TrneMDH transcript, and presence of CP-AMV transcript, support further analysis of these events for delayed leaf senescence under drought conditions, aluminium tolerance, and resistance to AMV. © 2020, Springer Nature B.V. 653 $aAgrobacterium-mediated transformation 653 $aDelayed leaf senescence 653 $aMultigene 653 $aT-DNA 700 1 $aDING, Y.-L. 700 1 $aLIN, Y.-H. 700 1 $aSAHAB, S. 700 1 $aPANTER, S. 700 1 $aHAYES, M. 700 1 $aJOHN, U. 700 1 $aANDERSON, H. 700 1 $aMASON, J. 700 1 $aSPANGENBERG, G. 773 $tPlant Cell, Tissue and Organ Culture, 2020. DOI: https://doi.org/10.1007/s11240-020-01891-6
Descargar
Esconder MarcPresentar Marc Completo |
Registro original : |
INIA Treinta y Tres (TT) |
|
No copia registrada de este documento.
|
Volver
|
|
Registros recuperados : 3 | |
1. | | SINGH, D.; ZIEMS, L.A.; DRACATOS, P.M.; POURKHEIRANDISH, M.; TSHEWANG, S.; CZEMBOR, P.; GERMAN, S.; FOWLER, R.A.; SNYMAN, L.; PLATZD, G.J.; PARK, R.F. Genome-wide association studies provide insights on genetic architecture of resistance to leaf rust in a worldwide barley collection. Molecular Breeding, 1 April 2018, Volume 38, Issue 4, Article number 43. Article history:Received: 16 August 2017 /Accepted: 13 March 2018.Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
Biblioteca(s): INIA La Estanzuela. |
| |
2. | | HICKEY, L.T.; GERMAN, S.; PEREYRA, S.; DIAZ-LAGO, J.E.; ZIEMS, L.A.; FOWLER, R.A.; PLATZ, G.J.; FRANCKOWIAK, J.D.; DIETERS, M.J. Speed breeding for multiple disease resistance in barley. Euphytica, v. 213, n.3, March 2017. Article history: Received: 22 April 2016 //Accepted: 18 November 2016 //Published online: 7 February 2017.Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
Biblioteca(s): INIA La Estanzuela. |
| |
3. | | HICKEY, L.T.; LAWSON, W.; PLATZ, G.J.; FOWLER, R.A; ARIEF, V.N.; DIETERS, M.; GERMAN, S.; FLETCHER, S.; PARK, R.F.; PEREYRA, S.; SINGH, D.; FRANCKOWIAK, J. Mapping quantitative trait loci for partial resistance to powdery mildew in an Australian barley population. Crop Science, v. 52, n. 3, p. 1021-1032, 2012.Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : A - 1 |
Biblioteca(s): INIA La Estanzuela. |
| |
Registros recuperados : 3 | |
|
Expresión de búsqueda válido. Check! |
|
|