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| 14. |  | FERNÁNDEZ ,S.; FRAGA, M.; CASTELLS, M.; COLINA, R.; ZUNINO,P. Effect of the administration of Lactobacillus spp. strains on neonatal diarrhoea, immune parameters and pathogen abundance in pre-weaned calves. Beneficial Microbes, 1 September 2020, Volume 11, Issue 5, Pages 477-488. Doi: https://doi.org/10.3920/BM2019.0167| Biblioteca(s): INIA La Estanzuela. |
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| 20. | | QUERO, G.; BONNECARRERE, V.; FERNÁNDEZ, S.; SILVA, P.; SIMONDI, S.; BORSANI, O. La eficiencia del uso de luz y la partición energética en arroz es genotipo dependiente. [resumen] In: INIA (Instituto Nacional de Investigación Agropecuaria); INIA Las Brujas; Biotecnología. Jornada de Agrobiotecnología, XI. Encuentro Nacional de REDBIO, III. Jornada técnica. Las Brujas, Canelones (UY): INIA, 2018. p.8-9 (Serie Actividades de Difusión; 786)| Biblioteca(s): INIA Las Brujas. |
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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
22/07/2018 |
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Actualizado : |
22/07/2018 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
FERNÁNDEZ, S.; FRAGA, M.; SILVEYRA, E.; TROMBERT, A.N.; RABAZA, A.; PLA, M. |
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Afiliación : |
S. FERNÁNDEZ, MEC/ IIBCE (Instituto de Investigaciones de Ciencias Biológicas "Clemente Estable"); MARTIN FRAGA COTELO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; E. SILVEYRA, MEC/ IIBCE (Instituto de Investigaciones de Ciencias Biológicas "Clemente Estable"); A.N. TROMBERT, Universidad Mayor, Santiago (Chile); A. RABAZA, MEC/ IIBCE (Instituto de Investigaciones de Ciencias Biológicas "Clemente Estable"); MARCELO PLA TEJERA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Probiotic properties of native Lactobacillus spp. strains for dairy calves. |
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Complemento del título : |
Research article. |
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Fecha de publicación : |
2018 |
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Fuente / Imprenta : |
Beneficial Microbes, April 2018, vol.9, no.4, p. 613 - 624. |
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ISSN : |
1876-2883 |
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DOI : |
10.3920/BM2017.0131 |
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Idioma : |
Inglés |
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Notas : |
Artilce history: Received: 12 September 2017 / Accepted: 22 December 2017 / Published Online: April 10, 2018. |
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Contenido : |
ABSTRACT.
The use of native microorganisms with probiotic capacity is an alternative tool for the treatment and prevention of several diseases that affect animals, such as neonatal calf diarrhoea. The selection of probiotic strains within a collection is based on different in vitro and in vivo assays, which predict their potential. The aim of this study was to characterise a group of native Lactobacillus spp. strains isolated from faeces of healthy calves using an in vitro approach and to assess their ability to colonise the gastrointestinal tract (GIT) of calves. Native Lactobacillus spp. strains were evaluated on their capacity to survive low pH conditions and bile salts presence, biofilm formation and adhesion to both mucus and Caco-2 cells. Based on the in vitro characterisation, four strains (Lactobacillus johnsonii TP1.1, Lactobacillus reuteri TP1.3B, L. johnsonii TP1.6 and Lactobacillus amylovorus TP8.7) were selected to evaluate their capacity to colonise and persist in the GIT of calves. The assessment of enteric persistence involved an in vivo assay with oral administration of probiotics and quantification in faeces of the administered bacterial species with real-time quantitative PCR (qPCR). The study was conducted using 15 calves (1-month-old) which were divided into five groups of three animals, four of which were treated with four different selected strains and one was the control group. Strains TP1.3B and TP1.6 managed to persist in treated animals until ten days after the end of the administration period, indicating that they could be promising candidates for the design of probiotics for calves.
© 2018 Wageningen Academic Publishers. MenosABSTRACT.
The use of native microorganisms with probiotic capacity is an alternative tool for the treatment and prevention of several diseases that affect animals, such as neonatal calf diarrhoea. The selection of probiotic strains within a collection is based on different in vitro and in vivo assays, which predict their potential. The aim of this study was to characterise a group of native Lactobacillus spp. strains isolated from faeces of healthy calves using an in vitro approach and to assess their ability to colonise the gastrointestinal tract (GIT) of calves. Native Lactobacillus spp. strains were evaluated on their capacity to survive low pH conditions and bile salts presence, biofilm formation and adhesion to both mucus and Caco-2 cells. Based on the in vitro characterisation, four strains (Lactobacillus johnsonii TP1.1, Lactobacillus reuteri TP1.3B, L. johnsonii TP1.6 and Lactobacillus amylovorus TP8.7) were selected to evaluate their capacity to colonise and persist in the GIT of calves. The assessment of enteric persistence involved an in vivo assay with oral administration of probiotics and quantification in faeces of the administered bacterial species with real-time quantitative PCR (qPCR). The study was conducted using 15 calves (1-month-old) which were divided into five groups of three animals, four of which were treated with four different selected strains and one was the control group. Strains TP1.3B and TP1.6 managed to persist in treated animals until ten d... Presentar Todo |
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Palabras claves : |
GASTROINTESTINAL TRACT; LACTOBACILLUS SPP; NATIVE; NEONATAL CALF DIARRHOEA; PROBIOTIC POTENTIAL. |
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Thesagro : |
GANADO DE LECHE. |
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Asunto categoría : |
-- |
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Marc : |
LEADER 02595naa a2200289 a 4500
001 1058830
005 2018-07-22
008 2018 bl uuuu u00u1 u #d
022 $a1876-2883
024 7 $a10.3920/BM2017.0131$2DOI
100 1 $aFERNÁNDEZ, S.
245 $aProbiotic properties of native Lactobacillus spp. strains for dairy calves.$h[electronic resource]
260 $c2018
500 $aArtilce history: Received: 12 September 2017 / Accepted: 22 December 2017 / Published Online: April 10, 2018.
520 $aABSTRACT. The use of native microorganisms with probiotic capacity is an alternative tool for the treatment and prevention of several diseases that affect animals, such as neonatal calf diarrhoea. The selection of probiotic strains within a collection is based on different in vitro and in vivo assays, which predict their potential. The aim of this study was to characterise a group of native Lactobacillus spp. strains isolated from faeces of healthy calves using an in vitro approach and to assess their ability to colonise the gastrointestinal tract (GIT) of calves. Native Lactobacillus spp. strains were evaluated on their capacity to survive low pH conditions and bile salts presence, biofilm formation and adhesion to both mucus and Caco-2 cells. Based on the in vitro characterisation, four strains (Lactobacillus johnsonii TP1.1, Lactobacillus reuteri TP1.3B, L. johnsonii TP1.6 and Lactobacillus amylovorus TP8.7) were selected to evaluate their capacity to colonise and persist in the GIT of calves. The assessment of enteric persistence involved an in vivo assay with oral administration of probiotics and quantification in faeces of the administered bacterial species with real-time quantitative PCR (qPCR). The study was conducted using 15 calves (1-month-old) which were divided into five groups of three animals, four of which were treated with four different selected strains and one was the control group. Strains TP1.3B and TP1.6 managed to persist in treated animals until ten days after the end of the administration period, indicating that they could be promising candidates for the design of probiotics for calves. © 2018 Wageningen Academic Publishers.
650 $aGANADO DE LECHE
653 $aGASTROINTESTINAL TRACT
653 $aLACTOBACILLUS SPP
653 $aNATIVE
653 $aNEONATAL CALF DIARRHOEA
653 $aPROBIOTIC POTENTIAL
700 1 $aFRAGA, M.
700 1 $aSILVEYRA, E.
700 1 $aTROMBERT, A.N.
700 1 $aRABAZA, A.
700 1 $aPLA, M.
773 $tBeneficial Microbes, April 2018, vol.9, no.4, p. 613 - 624.
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