|
|
| Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
Registro completo
|
Biblioteca (s) : |
INIA La Estanzuela. |
Fecha : |
21/02/2014 |
Actualizado : |
30/10/2019 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
MORON, A.; GARCIA, A.; SAWCHIK, J.; COZZOLINO, D. |
Afiliación : |
DAVID ALEJANDRO MORON YACOEL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ADRIANA GARCIA LAMOTHE, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JORGE SAWCHIK PINTOS, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; DANIEL COZZOLINO GÓMEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
Preliminary study on the use of near-infrared reflectance spectroscopy to assess nitrogen content of undried wheat plants. |
Fecha de publicación : |
2007 |
Fuente / Imprenta : |
Journal of the Science of Food and Agriculture, 15 January 2007, Volume 87, Issue 1, Pages 147-152. |
DOI : |
10.1002/jsfa.2691 |
Idioma : |
Inglés |
Notas : |
Article history:Received 11 August 2005/ Revised version received 16 January 2006/ Accepted 16 August 2006. |
Contenido : |
Abstract: Near-infrared reflectance (NIR) spectroscopy combined with chemometrics was used to assess nitrogen (N) and dry matter content (DM) and chlorophyll in whole-wheat plant (Triticum aestivum L). Whole-wheat plant samples (n = 245) were analysed by reference method and by visible and NIR spectroscopy, in fresh (n = 182) and dry (n = 63) presentations, respectively. Calibration equations were developed using partial least squares (PLS) and validated using full cross-validation (leave-one-out method). Coefficient of determination in calibration (R2CAL) and the standard error of cross-validation (SECV) for N content in fresh sample presentation, after second derivative, were 0.89 (SECV: 0.64%), 0.86 (SECV: 0.66%) and 0.82 (SECV: 0.74%) using the visible + NIR, NIR and
visible wavelength regions, respectively. Dry sample presentation gave better R2 CAL and SECV for N compared with fresh presentation (R2 CAL > 0.90, SECV <0.20%) using visible + NIR. The results demonstrated that NIR is a suitable method to assess N concentration in wheat plant using fresh samples (unground and undried). |
Palabras claves : |
DRY MATTER; FORRAJE NO SECO; MATERIA SECA; NEAR-INFRARED SPECTROSCOPY; NITROGEN; PARTIAL LEAST SQUARES; UNDRIED FORAGE; WHEAT. |
Thesagro : |
NIRS; TRIGO. |
Asunto categoría : |
F01 Cultivo |
Marc : |
LEADER 02083naa a2200301 a 4500 001 1049576 005 2019-10-30 008 2007 bl uuuu u00u1 u #d 024 7 $a10.1002/jsfa.2691$2DOI 100 1 $aMORON, A. 245 $aPreliminary study on the use of near-infrared reflectance spectroscopy to assess nitrogen content of undried wheat plants. 260 $c2007 500 $aArticle history:Received 11 August 2005/ Revised version received 16 January 2006/ Accepted 16 August 2006. 520 $aAbstract: Near-infrared reflectance (NIR) spectroscopy combined with chemometrics was used to assess nitrogen (N) and dry matter content (DM) and chlorophyll in whole-wheat plant (Triticum aestivum L). Whole-wheat plant samples (n = 245) were analysed by reference method and by visible and NIR spectroscopy, in fresh (n = 182) and dry (n = 63) presentations, respectively. Calibration equations were developed using partial least squares (PLS) and validated using full cross-validation (leave-one-out method). Coefficient of determination in calibration (R2CAL) and the standard error of cross-validation (SECV) for N content in fresh sample presentation, after second derivative, were 0.89 (SECV: 0.64%), 0.86 (SECV: 0.66%) and 0.82 (SECV: 0.74%) using the visible + NIR, NIR and visible wavelength regions, respectively. Dry sample presentation gave better R2 CAL and SECV for N compared with fresh presentation (R2 CAL > 0.90, SECV <0.20%) using visible + NIR. The results demonstrated that NIR is a suitable method to assess N concentration in wheat plant using fresh samples (unground and undried). 650 $aNIRS 650 $aTRIGO 653 $aDRY MATTER 653 $aFORRAJE NO SECO 653 $aMATERIA SECA 653 $aNEAR-INFRARED SPECTROSCOPY 653 $aNITROGEN 653 $aPARTIAL LEAST SQUARES 653 $aUNDRIED FORAGE 653 $aWHEAT 700 1 $aGARCIA, A. 700 1 $aSAWCHIK, J. 700 1 $aCOZZOLINO, D. 773 $tJournal of the Science of Food and Agriculture, 15 January 2007, Volume 87, Issue 1, Pages 147-152.
Descargar
Esconder MarcPresentar Marc Completo |
Registro original : |
INIA La Estanzuela (LE) |
|
Biblioteca
|
Identificación
|
Origen
|
Tipo / Formato
|
Clasificación
|
Cutter
|
Registro
|
Volumen
|
Estado
|
Volver
|
|
Registro completo
|
Biblioteca (s) : |
INIA La Estanzuela. |
Fecha actual : |
01/11/2018 |
Actualizado : |
07/11/2018 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Circulación / Nivel : |
Internacional - -- |
Autor : |
ZARANTONELLI, L.; SUANES, A.; MENY, P.; BURONI, F.; SALVARREY,X.; BRIANO , C.; ASHFIELD, N.; SILVEIRA, C.S.; DUTRA, F.; EASTON, C.; FRAGA, M.; GIANNITTI, F.; HAMOND, C.; MACÍAS-RIOSECO, M.; MENÉNDEZ, C.; MORTOLA, A.; PICARDEAU, M. |
Afiliación : |
LETICIA ZARANTONELLI, Laboratorio de Microbiología Molecular y Estructural, Institut Pasteur de Montevideo, Uruguay.; Unidad Mixta UMPI, Institut Pasteur de Montevideo; INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay.; ALEJANDRA SUANES, Departamento de Bacteriología, División Laboratorios Veterinarios "Miguel C. Rubino" Sede Central, Ministerio de Ganadería, Agricultura y Pesca, Uruguay.; PAULINA MENY, Departamento de Bacteriología y Virología, Instituto de Higiene, Facultad de Medicina, Universidad de la República, Uruguay.; FLORENCIA BURONI, División Laboratorios Veterinarios "Miguel C. Rubino" Laboratorio Regional Noroeste, Ministerio de Ganadería, Agricultura y Pesca, Uruguay.; XIMENA SALVARREY, Departamento de Bacteriología, División Laboratorios Veterinarios "Miguel C. Rubino". Sede Central, Ministerio de Ganadería, Agricultura y Pesca, Uruguay .; CAROLINA BRIANO, Departamento de Bacteriología, División Laboratorios Veterinarios "Miguel C. Rubino" Sede Central, Ministerio de Ganadería, Agricultura y Pesca, Uruguay .; NATALIA ASHFIELD4, Departamento de Bacteriología y Virologí, Instituto de Higiene, Facultad de Medicina, Universidad de la República, Uruguay.; CAROLINE DA SILVA SILVEIRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FERNANDO DUTRA, División Laboratorios Veterinarios "Miguel C. Rubino". Laboratorio Regional Este, Ministerio de Ganadería, Agricultura y Pesca, Uruguay.; CRISTINA EASTON, Departamento de Bacteriología, División Laboratorios Veterinarios "Miguel C. Rubino" Sede Central, Ministerio de Ganadería, Agricultura y Pesca, Uruguay.; MARTIN FRAGA COTELO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FEDERICO GIANNITTI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CAMILA HAMOND, Unidad Mixta UMPI, Institut Pasteur de Montevideo ; INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MELISSA MACÍAS RIOSECO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CLARA MENÉNDEZ, Departamento de Bacteriología y Virología, Instituto de Higiene, Facultad de Medicina, Universidad de la República, Uruguay.; ALBERTO MORTOLA, Departamento de Bacteriología, División Laboratorios Veterinarios "Miguel C. Rubino" Sede Central, Ministerio de Ganadería, Agricultura y Pesca, Uruguay.; MATHIEU PICARDEAU, Institut Pasteur de Montevideo, Uruguay. / Institut Pasteur, France. |
Título : |
Isolation of pathogenic Leptospira strains from naturally infected cattle in Uruguay reveals high serovar diversity, and uncovers a relevant risk for human leptospirosis. |
Fecha de publicación : |
2018 |
Fuente / Imprenta : |
PLoS Neglected Tropical Diseases, September 2018, vol. 12, Issue 9, Article number e0006694. OPEN ACCESS. |
DOI : |
10.1371/journal.pntd.0006694 |
Idioma : |
Inglés |
Notas : |
Article History: Received: February 8, 2018; Accepted: July 16, 2018; Published: September 13, 2018. |
Contenido : |
Abstract:
Leptospirosis is a neglected zoonosis with worldwide distribution. The causative agents are spirochete bacteria of the Leptospira genus, displaying huge diversity of serovars, the identity of which is critical for effective diagnosis and vaccination purposes. Among many other mammalian species, Leptospira infects cattle, eliciting acute signs in calves, and chronic disease in adult animals often leading to abortions. In South America, and including in Uruguay, beef and dairy export are leading sources of national income. Despite the importance of bovine health, food safety, and bovine-related dissemination of leptospirosis to humans, extremely limited information is available as to the identity of Leptospira species and serovars infecting cattle in Uruguay and the South American subcontinent. Here we report a multicentric 3-year study resulting in the isolation and detailed characterization of 40 strains of Leptospira spp. obtained from infected cattle. Combined serologic and molecular typing identified these isolates as L. interrogans serogroup Pomona serovar Kennewicki (20 strains), L. interrogans serogroup Canicola serovar Canicola (1 strain), L. borgpetersenii serogroup Sejroe serovar Hardjo (10 strains) and L. noguchii (9 strains). The latter showed remarkable phenotypic and genetic variability, belonging to 6 distinct serogroups, including 3 that did not react with a large panel of reference serogrouping antisera. Approximately 20% of cattle sampled in the field were found to be shedding pathogenic Leptospira in their urine, uncovering a threat for public health that is being largely neglected. The two L. interrogans serovars that we isolated from cattle displayed identical genetic signatures to those of human isolates that had previously been obtained from leptospirosis patients. This report of local Leptospira strains shall improve diagnostic tools and the understanding of leptospirosis epidemiology in South America. These strains could also be used as new components within bacterin vaccines to protect against the pathogenic Leptospira strains that are actually circulating, a direct measure to reduce the risk of human leptospirosis.
© 2018 Zarantonelli et al. http://creativecommons.org/licenses/by/4.0/. MenosAbstract:
Leptospirosis is a neglected zoonosis with worldwide distribution. The causative agents are spirochete bacteria of the Leptospira genus, displaying huge diversity of serovars, the identity of which is critical for effective diagnosis and vaccination purposes. Among many other mammalian species, Leptospira infects cattle, eliciting acute signs in calves, and chronic disease in adult animals often leading to abortions. In South America, and including in Uruguay, beef and dairy export are leading sources of national income. Despite the importance of bovine health, food safety, and bovine-related dissemination of leptospirosis to humans, extremely limited information is available as to the identity of Leptospira species and serovars infecting cattle in Uruguay and the South American subcontinent. Here we report a multicentric 3-year study resulting in the isolation and detailed characterization of 40 strains of Leptospira spp. obtained from infected cattle. Combined serologic and molecular typing identified these isolates as L. interrogans serogroup Pomona serovar Kennewicki (20 strains), L. interrogans serogroup Canicola serovar Canicola (1 strain), L. borgpetersenii serogroup Sejroe serovar Hardjo (10 strains) and L. noguchii (9 strains). The latter showed remarkable phenotypic and genetic variability, belonging to 6 distinct serogroups, including 3 that did not react with a large panel of reference serogrouping antisera. Approximately 20% of cattle sampled in the fi... Presentar Todo |
Palabras claves : |
SALUD ANIMAL. |
Thesagro : |
LEPTOSPIRA; LEPTOSPIROSIS. |
Asunto categoría : |
-- |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/11772/1/Zarantonelli-2018-Isolation-of-pathogenic-leptospira-1.pdf
|
Marc : |
LEADER 03472naa a2200373 a 4500 001 1059268 005 2018-11-07 008 2018 bl uuuu u00u1 u #d 024 7 $a10.1371/journal.pntd.0006694$2DOI 100 1 $aZARANTONELLI, L. 245 $aIsolation of pathogenic Leptospira strains from naturally infected cattle in Uruguay reveals high serovar diversity, and uncovers a relevant risk for human leptospirosis. 260 $c2018 500 $aArticle History: Received: February 8, 2018; Accepted: July 16, 2018; Published: September 13, 2018. 520 $aAbstract: Leptospirosis is a neglected zoonosis with worldwide distribution. The causative agents are spirochete bacteria of the Leptospira genus, displaying huge diversity of serovars, the identity of which is critical for effective diagnosis and vaccination purposes. Among many other mammalian species, Leptospira infects cattle, eliciting acute signs in calves, and chronic disease in adult animals often leading to abortions. In South America, and including in Uruguay, beef and dairy export are leading sources of national income. Despite the importance of bovine health, food safety, and bovine-related dissemination of leptospirosis to humans, extremely limited information is available as to the identity of Leptospira species and serovars infecting cattle in Uruguay and the South American subcontinent. Here we report a multicentric 3-year study resulting in the isolation and detailed characterization of 40 strains of Leptospira spp. obtained from infected cattle. Combined serologic and molecular typing identified these isolates as L. interrogans serogroup Pomona serovar Kennewicki (20 strains), L. interrogans serogroup Canicola serovar Canicola (1 strain), L. borgpetersenii serogroup Sejroe serovar Hardjo (10 strains) and L. noguchii (9 strains). The latter showed remarkable phenotypic and genetic variability, belonging to 6 distinct serogroups, including 3 that did not react with a large panel of reference serogrouping antisera. Approximately 20% of cattle sampled in the field were found to be shedding pathogenic Leptospira in their urine, uncovering a threat for public health that is being largely neglected. The two L. interrogans serovars that we isolated from cattle displayed identical genetic signatures to those of human isolates that had previously been obtained from leptospirosis patients. This report of local Leptospira strains shall improve diagnostic tools and the understanding of leptospirosis epidemiology in South America. These strains could also be used as new components within bacterin vaccines to protect against the pathogenic Leptospira strains that are actually circulating, a direct measure to reduce the risk of human leptospirosis. © 2018 Zarantonelli et al. http://creativecommons.org/licenses/by/4.0/. 650 $aLEPTOSPIRA 650 $aLEPTOSPIROSIS 653 $aSALUD ANIMAL 700 1 $aSUANES, A. 700 1 $aMENY, P. 700 1 $aBURONI, F. 700 1 $aSALVARREY,X. 700 1 $aBRIANO , C. 700 1 $aASHFIELD, N. 700 1 $aSILVEIRA, C.S. 700 1 $aDUTRA, F. 700 1 $aEASTON, C. 700 1 $aFRAGA, M. 700 1 $aGIANNITTI, F. 700 1 $aHAMOND, C. 700 1 $aMACÍAS-RIOSECO, M. 700 1 $aMENÉNDEZ, C. 700 1 $aMORTOLA, A. 700 1 $aPICARDEAU, M. 773 $tPLoS Neglected Tropical Diseases, September 2018, vol. 12, Issue 9, Article number e0006694. OPEN ACCESS.
Descargar
Esconder MarcPresentar Marc Completo |
Registro original : |
INIA La Estanzuela (LE) |
|
Biblioteca
|
Identificación
|
Origen
|
Tipo / Formato
|
Clasificación
|
Cutter
|
Registro
|
Volumen
|
Estado
|
Volver
|
Expresión de búsqueda válido. Check! |
|
|