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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
22/01/2020 |
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Actualizado : |
22/01/2020 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
SILVA, P.E.; RIET-CORREA, F.; COELHO, A.C.B.; ECHENIQUE, J.V.Z.; PEREIRA, C.M.; LIMA, M.; DIEL, D.G.; SCHILD, A.L. |
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Afiliación : |
P. ESTIMA-SILVA, Programa de pós-graduação, Faculdade de Veterinária, Universidade Federal de Pelotas - (UFPel), Pelotas, RS, Brazil; FRANKLIN RIET-CORREA AMARAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; A.C.B. COELHO, Programa de pós-graduação, Faculdade de Veterinária, Universidade Federal de Pelotas - (UFPel), Pelotas, RS, Brazil; J.V.Z. ECHENIQUE, Programa de pós-graduação, Faculdade de Veterinária, Universidade Federal de Pelotas - (UFPel), Pelotas, RS, Brazil; C. MARCOLONGO-PEREIRA, Faculdade de Veterinária, Centro Universitário do Espírito Santo (UNESC), Colatina, ES, Brazil; M. LIMA, Faculdade de Veterinária (FV), Universidade Federal de Pelotas (UFPel), Pelotas, RS, Brazil; D.G. DIEL, South Dakota State University, Brookings, Brazil; A.L. SCHILD, Faculdade de Veterinária (FV), Universidade Federal de Pelotas (UFPel), Pelotas, RS, Brazil. |
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Título : |
Identification of equine herpesvirus type 1 as cause of abortion in mares in Southern Brazil. (Communication). [Identificação do herpesvirus equino tipo 1 como causa de abortos em éguas no sul do Brasil]. (Comunicação) |
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Fecha de publicación : |
2019 |
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Fuente / Imprenta : |
Arquivo Brasileiro de Medicina Veterinaria e Zootecnia, 2019, Volume 71, Issue 4, Pages 1421-1424. OPEN ACCESS. Doi: 10.1590/1678-4162-10430 |
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ISSN : |
0102-0935 |
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DOI : |
10.1590/1678-4162-10430 |
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Idioma : |
Inglés |
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Notas : |
Article history: Recebido em 20 de dezembro de 2017 / Aceito em 6 de outubro de 2018. |
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Contenido : |
RESUMO.
Foi realizado um estudo retrospectivo dos casos suspeitos de aborto por herpesvírus equino observados na região sul do Rio Grande do Sul entre 1978 e 2016. Foram revisados os protocolos de necropsia do Laboratório Regional de Diagnóstico da Faculdade de Veterinária da Universidade Federal de Pelotas resgatando-se os dados epidemiológicos, lesões macroscópicas e histológicas de cada caso. Foram observados dois surtos da enfermidade com prevalência entre 5,7% e 50% nos diferentes
estabelecimentos, e dois casos individuais. Em todos os casos foram enviados fragmentos de órgãos fetais formolizados. Histologicamente, em todos os casos foram observados focos de necrose no fígado, pulmão e baço e presença de corpúsculos de inclusão acidofílico em hepatócitos, células epiteliais pulmonares e leucócitos. A imuno-histoquímica utilizando anticorpo policlonal comercial para herpesvirus equino-1 (EHV-1) revelou marcação positiva em todos os casos. Além disso, foi extraído
DNA dos tecidos emblocados em parafina dos casos e submetidos à técnica de nested-PCR seguida de sequenciamento genômico dos amplicons em duas amostras. Estes achados indicam, que EHV-1 deve ser considerado como diagnóstico diferencial em casos de aborto em equinos no sul do Rio Grande do Sul. |
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Palabras claves : |
ABORTION; EQUINE HERPESVIRUS-1; HERPESVIRUS EQUINO-1; IMMUNOHISTOCHEMISTRY; IMUNO-HISTOQUÍMICA; INFECTION; PLATAFORMA SALUD ANIMAL. |
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Thesagro : |
ABORTO; PCR. |
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Asunto categoría : |
L73 Enfermedades de los animales |
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URL : |
http://www.scielo.br/pdf/abmvz/v71n4/0102-0935-abmvz-71-04-1421.pdf
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Marc : |
LEADER 02577naa a2200349 a 4500
001 1060663
005 2020-01-22
008 2019 bl uuuu u00u1 u #d
022 $a0102-0935
024 7 $a10.1590/1678-4162-10430$2DOI
100 1 $aSILVA, P.E.
245 $aIdentification of equine herpesvirus type 1 as cause of abortion in mares in Southern Brazil. (Communication). [Identificação do herpesvirus equino tipo 1 como causa de abortos em éguas no sul do Brasil]. (Comunicação)$h[electronic resource]
260 $c2019
500 $aArticle history: Recebido em 20 de dezembro de 2017 / Aceito em 6 de outubro de 2018.
520 $aRESUMO. Foi realizado um estudo retrospectivo dos casos suspeitos de aborto por herpesvírus equino observados na região sul do Rio Grande do Sul entre 1978 e 2016. Foram revisados os protocolos de necropsia do Laboratório Regional de Diagnóstico da Faculdade de Veterinária da Universidade Federal de Pelotas resgatando-se os dados epidemiológicos, lesões macroscópicas e histológicas de cada caso. Foram observados dois surtos da enfermidade com prevalência entre 5,7% e 50% nos diferentes estabelecimentos, e dois casos individuais. Em todos os casos foram enviados fragmentos de órgãos fetais formolizados. Histologicamente, em todos os casos foram observados focos de necrose no fígado, pulmão e baço e presença de corpúsculos de inclusão acidofílico em hepatócitos, células epiteliais pulmonares e leucócitos. A imuno-histoquímica utilizando anticorpo policlonal comercial para herpesvirus equino-1 (EHV-1) revelou marcação positiva em todos os casos. Além disso, foi extraído DNA dos tecidos emblocados em parafina dos casos e submetidos à técnica de nested-PCR seguida de sequenciamento genômico dos amplicons em duas amostras. Estes achados indicam, que EHV-1 deve ser considerado como diagnóstico diferencial em casos de aborto em equinos no sul do Rio Grande do Sul.
650 $aABORTO
650 $aPCR
653 $aABORTION
653 $aEQUINE HERPESVIRUS-1
653 $aHERPESVIRUS EQUINO-1
653 $aIMMUNOHISTOCHEMISTRY
653 $aIMUNO-HISTOQUÍMICA
653 $aINFECTION
653 $aPLATAFORMA SALUD ANIMAL
700 1 $aRIET-CORREA, F.
700 1 $aCOELHO, A.C.B.
700 1 $aECHENIQUE, J.V.Z.
700 1 $aPEREIRA, C.M.
700 1 $aLIMA, M.
700 1 $aDIEL, D.G.
700 1 $aSCHILD, A.L.
773 $tArquivo Brasileiro de Medicina Veterinaria e Zootecnia, 2019, Volume 71, Issue 4, Pages 1421-1424. OPEN ACCESS. Doi: 10.1590/1678-4162-10430
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INIA Las Brujas (LB) |
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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
21/02/2014 |
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Actualizado : |
05/02/2020 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
A - 2 |
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Autor : |
QUAGLIOTTO, L.; AZZIZ, G.; BAJSA, N.; VAZ, P.; PÉREZ, C.; DUCAMP, F.; CADENAZZI, M.; ALTIER, N.; ARIAS, A. |
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Afiliación : |
LETICIA QUAGLIOTTO, Laboratorio de Ecología Microbiana, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay; GASTÓN AZZIZ, Laboratorio de Ecología Microbiana, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay; NATALIA BAJSA, Laboratorio de Ecología Microbiana, Instituto de Investigaciones Biológicas Clemente Estable, ¡Montevideo, Uruguay; Sección Bioquímica, Facultad de Ciencias, UdelaR, Montevideo, Uruguay; PATRICIA VAZ, Laboratorio de Ecología Microbiana, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay; CARLOS PÉREZ, Departamento de Protección Vegetal, Facultad de Agronomía, Universidad de la República, Paysandú, Uruguay; FERNANDO DUCAMPO, Departamento de Protección Vegetal, Facultad de Agronomía, Universidad de la República, Paysandú, Uruguay; MÓNICA CADENAZZI, Departamento de Protección Vegetal, Facultad de Agronomía, Universidad de la República, Paysandú, Uruguay; NORA ADRIANA ALTIER MANZINI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ALICIA ARIAS, Laboratorio de Ecología Microbiana, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay. |
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Título : |
Three native Pseudomonas fluorescens strains tested under growth chamber and field conditions as biocontrol agents against damping-off in alfalfa. |
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Fecha de publicación : |
2009 |
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Fuente / Imprenta : |
Biological Control, October 2009, Volume 51, Issue 1, Pages 42-50. Doi: https://doi.org/10.1016/j.biocontrol.2009.05.006 |
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ISSN : |
1049-9644 |
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DOI : |
10.1016/j.biocontrol.2009.05.006 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 1 September 2008 / Accepted 22 May 2009 / Available online 28 May 2009.
Corresponding author: Leticia Quagliotto - letty@iibce.edu.uy
Funding information: This work was financially supported by the LIA-INIA 028 Project, in the frame of the "Programa de Servicios Agropecuarios 1131-OC-UR (BID-MGAP-INIA)". |
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Contenido : |
ABSTRACT.
Alfalfa (Medicago sativa) is one of the most important crops used in Uruguay for livestock feeding. Seedling diseases, particularly damping-off, are a critical factor which limits its establishment. Three native Pseudomonas fluorescens strains, UP61.2, UP143.8 and UP148.2, previously isolated from Lotus corniculatus, were evaluated to determine their efficacy as biological control agents for alfalfa seedling diseases in the field. Their compatibility with the alfalfa-Sinorhizobium meliloti symbiosis was also assessed. In growth chamber conditions seed inoculation with Pseudomonas strains did not affect different parameters of alfalfa-rhizobium symbiosis as shown by nodulation rate and shoot dry weight of plants. The presence of the commercial inoculant strains of S. meliloti did not impair colonization by the P. fluorescens and vice versa. In field trials the dynamics of rhizobial rhizospheric populations were not affected by the presence of P. fluorescens. Each P. fluorescens strain successfully colonized alfalfa roots at adequate densities for biocontrol activity. Results showed that P. fluorescens strains provided a 10-13% increase in the number of established plants relative to the control, an intermediate result compared to the fungicide treatment (24%). The alfalfa above-ground biomass was increased by 13% and 15-18% in the presence of the fungicide and P. fluorescens strains, respectively. Therefore, results from this study demonstrated that the three P. fluorescens strains provided effective control against soil-borne pathogens and suggest a potential use in the development of a commercial inoculant to be applied for the control of legume seedling diseases.
© 2009 Elsevier Inc. All rights reserved. MenosABSTRACT.
Alfalfa (Medicago sativa) is one of the most important crops used in Uruguay for livestock feeding. Seedling diseases, particularly damping-off, are a critical factor which limits its establishment. Three native Pseudomonas fluorescens strains, UP61.2, UP143.8 and UP148.2, previously isolated from Lotus corniculatus, were evaluated to determine their efficacy as biological control agents for alfalfa seedling diseases in the field. Their compatibility with the alfalfa-Sinorhizobium meliloti symbiosis was also assessed. In growth chamber conditions seed inoculation with Pseudomonas strains did not affect different parameters of alfalfa-rhizobium symbiosis as shown by nodulation rate and shoot dry weight of plants. The presence of the commercial inoculant strains of S. meliloti did not impair colonization by the P. fluorescens and vice versa. In field trials the dynamics of rhizobial rhizospheric populations were not affected by the presence of P. fluorescens. Each P. fluorescens strain successfully colonized alfalfa roots at adequate densities for biocontrol activity. Results showed that P. fluorescens strains provided a 10-13% increase in the number of established plants relative to the control, an intermediate result compared to the fungicide treatment (24%). The alfalfa above-ground biomass was increased by 13% and 15-18% in the presence of the fungicide and P. fluorescens strains, respectively. Therefore, results from this study demonstrated that the three P. flu... Presentar Todo |
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Palabras claves : |
Biological control agent; Damping-off; Field trials; Growth chamber conditions. |
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Thesagro : |
CONTROL BIOLÓGICO; MEDICAGO SATIVA; PSEUDOMONAS FLUORESCENS. |
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Asunto categoría : |
F01 Cultivo |
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Marc : |
LEADER 03149naa a2200337 a 4500
001 1012817
005 2020-02-05
008 2009 bl uuuu u00u1 u #d
022 $a1049-9644
024 7 $a10.1016/j.biocontrol.2009.05.006$2DOI
100 1 $aQUAGLIOTTO, L.
245 $aThree native Pseudomonas fluorescens strains tested under growth chamber and field conditions as biocontrol agents against damping-off in alfalfa.$h[electronic resource]
260 $c2009
500 $aArticle history: Received 1 September 2008 / Accepted 22 May 2009 / Available online 28 May 2009. Corresponding author: Leticia Quagliotto - letty@iibce.edu.uy Funding information: This work was financially supported by the LIA-INIA 028 Project, in the frame of the "Programa de Servicios Agropecuarios 1131-OC-UR (BID-MGAP-INIA)".
520 $aABSTRACT. Alfalfa (Medicago sativa) is one of the most important crops used in Uruguay for livestock feeding. Seedling diseases, particularly damping-off, are a critical factor which limits its establishment. Three native Pseudomonas fluorescens strains, UP61.2, UP143.8 and UP148.2, previously isolated from Lotus corniculatus, were evaluated to determine their efficacy as biological control agents for alfalfa seedling diseases in the field. Their compatibility with the alfalfa-Sinorhizobium meliloti symbiosis was also assessed. In growth chamber conditions seed inoculation with Pseudomonas strains did not affect different parameters of alfalfa-rhizobium symbiosis as shown by nodulation rate and shoot dry weight of plants. The presence of the commercial inoculant strains of S. meliloti did not impair colonization by the P. fluorescens and vice versa. In field trials the dynamics of rhizobial rhizospheric populations were not affected by the presence of P. fluorescens. Each P. fluorescens strain successfully colonized alfalfa roots at adequate densities for biocontrol activity. Results showed that P. fluorescens strains provided a 10-13% increase in the number of established plants relative to the control, an intermediate result compared to the fungicide treatment (24%). The alfalfa above-ground biomass was increased by 13% and 15-18% in the presence of the fungicide and P. fluorescens strains, respectively. Therefore, results from this study demonstrated that the three P. fluorescens strains provided effective control against soil-borne pathogens and suggest a potential use in the development of a commercial inoculant to be applied for the control of legume seedling diseases. © 2009 Elsevier Inc. All rights reserved.
650 $aCONTROL BIOLÓGICO
650 $aMEDICAGO SATIVA
650 $aPSEUDOMONAS FLUORESCENS
653 $aBiological control agent
653 $aDamping-off
653 $aField trials
653 $aGrowth chamber conditions
700 1 $aAZZIZ, G.
700 1 $aBAJSA, N.
700 1 $aVAZ, P.
700 1 $aPÉREZ, C.
700 1 $aDUCAMP, F.
700 1 $aCADENAZZI, M.
700 1 $aALTIER, N.
700 1 $aARIAS, A.
773 $tBiological Control, October 2009, Volume 51, Issue 1, Pages 42-50. Doi: https://doi.org/10.1016/j.biocontrol.2009.05.006
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