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Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
01/10/2019 |
Actualizado : |
01/10/2019 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
CASTILLO, A.; CARRO, B. L.; DALLA RIZZA, M.; REYNO, R. |
Afiliación : |
ALICIA MARIA CASTILLO SALLE, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; B. LÓPEZ CARRO, Flow Cytometry and Cell Sorting Core, Institute of Biological Research Clemente Estable, Montevideo, Uruguay.; MARCO DALLA RIZZA VILARO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RAFAEL ALEJANDRO REYNO PODESTA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
Use of in vitro methods to induce autotetraploids in the native forage legume Trifolium polymorphum. |
Complemento del título : |
Conference Paper. |
Fecha de publicación : |
2018 |
Fuente / Imprenta : |
Acta Horticulturae, 15 November 2018, N° 1224, p. 73-80. |
Serie : |
(Acta Horticulturae; 1224) |
ISBN : |
978-94-62612-20-4 |
ISSN : |
0567-7572 (print) / 2406-6168 (electronic) |
DOI : |
10.17660/ActaHortic.2018.1224.11 |
Idioma : |
Inglés |
Notas : |
In: Acta Hortic. 1224. VII International Symposium on Production and Establishment of Micropropagated Plants. Editors: R. Paiva, M.V. Reis, D.P.C. Silva. |
Contenido : |
ABSTRACT.
The manipulation of ploidy is a helpful tool in improving crops and grasses. Trifolium polymorphum (Poir.) (Leguminosae) is a native perennial stoloniferous winter species that combines different types of reproductive systems. This biological advantage makes T. polymorphum a remarkable issue of research. T. polymorphum
belongs to the Campos region and has evolved into adapted ecotypes through long exposure to environmental stresses, grazing and by competing with the native C3 and C4 grasses which have dominated the Campos vegetation over a long period of time. Eurasiatic Trifolium species lack persistence in the temperate to subtropical
environment found in the Campos region and have to rely on their reseeding capacity to persist. T. polymorphum is currently being studied for its ability to adapt to
Uruguayan conditions. Despite this significant advantage in survival, the major constraint of T. polymorphum is a poor biomass production and low seedling vigor
that represents a limitation for its use as a forage legume. The objective of this work was to evaluate different in vitro methods to induce polyploidy. We evaluated two
antimitotic agents in different concentrations and time exposures: colchicine and oryzalin in different kinds of explant, and the effect of colchicine added in the culture
medium. Colchicine was more effective in polyploidy induction. In seedlings 0.3% of colchicine for 24 h achieved 11% of tetraploids, for in vitro explants 0.2% of
colchicine reached to 13% of chromosome duplication, and 16% when colchicine (0.015%) was added in the culture medium. The numbers of chloroplasts in stomatal
guard cells was used as a quick assessment of the ploidy level and then confirmed by flow cytometry.
@ISHS, International Society for Horticultural Science. MenosABSTRACT.
The manipulation of ploidy is a helpful tool in improving crops and grasses. Trifolium polymorphum (Poir.) (Leguminosae) is a native perennial stoloniferous winter species that combines different types of reproductive systems. This biological advantage makes T. polymorphum a remarkable issue of research. T. polymorphum
belongs to the Campos region and has evolved into adapted ecotypes through long exposure to environmental stresses, grazing and by competing with the native C3 and C4 grasses which have dominated the Campos vegetation over a long period of time. Eurasiatic Trifolium species lack persistence in the temperate to subtropical
environment found in the Campos region and have to rely on their reseeding capacity to persist. T. polymorphum is currently being studied for its ability to adapt to
Uruguayan conditions. Despite this significant advantage in survival, the major constraint of T. polymorphum is a poor biomass production and low seedling vigor
that represents a limitation for its use as a forage legume. The objective of this work was to evaluate different in vitro methods to induce polyploidy. We evaluated two
antimitotic agents in different concentrations and time exposures: colchicine and oryzalin in different kinds of explant, and the effect of colchicine added in the culture
medium. Colchicine was more effective in polyploidy induction. In seedlings 0.3% of colchicine for 24 h achieved 11% of tetraploids, for in vitro explants 0.2% of
colchicine r... Presentar Todo |
Palabras claves : |
BIOMASS; COLCHICINE; ORYZALIN; POLYPLOIDY. |
Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
Marc : |
LEADER 02755naa a2200265 a 4500 001 1060254 005 2019-10-01 008 2018 bl uuuu u00u1 u #d 020 $a978-94-62612-20-4 022 $a0567-7572 (print) / 2406-6168 (electronic) 024 7 $a10.17660/ActaHortic.2018.1224.11$2DOI 100 1 $aCASTILLO, A. 245 $aUse of in vitro methods to induce autotetraploids in the native forage legume Trifolium polymorphum.$h[electronic resource] 260 $c2018 490 $a(Acta Horticulturae; 1224) 500 $aIn: Acta Hortic. 1224. VII International Symposium on Production and Establishment of Micropropagated Plants. Editors: R. Paiva, M.V. Reis, D.P.C. Silva. 520 $aABSTRACT. The manipulation of ploidy is a helpful tool in improving crops and grasses. Trifolium polymorphum (Poir.) (Leguminosae) is a native perennial stoloniferous winter species that combines different types of reproductive systems. This biological advantage makes T. polymorphum a remarkable issue of research. T. polymorphum belongs to the Campos region and has evolved into adapted ecotypes through long exposure to environmental stresses, grazing and by competing with the native C3 and C4 grasses which have dominated the Campos vegetation over a long period of time. Eurasiatic Trifolium species lack persistence in the temperate to subtropical environment found in the Campos region and have to rely on their reseeding capacity to persist. T. polymorphum is currently being studied for its ability to adapt to Uruguayan conditions. Despite this significant advantage in survival, the major constraint of T. polymorphum is a poor biomass production and low seedling vigor that represents a limitation for its use as a forage legume. The objective of this work was to evaluate different in vitro methods to induce polyploidy. We evaluated two antimitotic agents in different concentrations and time exposures: colchicine and oryzalin in different kinds of explant, and the effect of colchicine added in the culture medium. Colchicine was more effective in polyploidy induction. In seedlings 0.3% of colchicine for 24 h achieved 11% of tetraploids, for in vitro explants 0.2% of colchicine reached to 13% of chromosome duplication, and 16% when colchicine (0.015%) was added in the culture medium. The numbers of chloroplasts in stomatal guard cells was used as a quick assessment of the ploidy level and then confirmed by flow cytometry. @ISHS, International Society for Horticultural Science. 653 $aBIOMASS 653 $aCOLCHICINE 653 $aORYZALIN 653 $aPOLYPLOIDY 700 1 $aCARRO, B. L. 700 1 $aDALLA RIZZA, M. 700 1 $aREYNO, R. 773 $tActa Horticulturae, 15 November 2018, N° 1224, p. 73-80.
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INIA Las Brujas (LB) |
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Biblioteca (s) : |
INIA Tacuarembó. |
Fecha actual : |
21/02/2014 |
Actualizado : |
10/07/2018 |
Tipo de producción científica : |
Artículos en Revistas Agropecuarias |
Autor : |
LUZARDO, S.; CUADRO, R. |
Afiliación : |
SANTIAGO FELIPE LUZARDO VILLAR, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; WASHINGTON ROBIN CUADRO LOPEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
El uso de pasturas sembradas y suplementación en la recría de terneros machos en el Basalto. |
Fecha de publicación : |
2018 |
Fuente / Imprenta : |
Revista INIA Uruguay, 2018, no. 53, p. 18-21. |
Serie : |
(Revista INIA; 53) |
ISSN : |
1510-9011 |
Idioma : |
Español |
Contenido : |
Existen diferentes estrategias de intensificación de la recría, como por ejemplo el creep feeding, el pastoreo de campo natural diferido, la suplementación estratégica
sobre campo natural, y tal vez lo más intensivo, el uso de pasturas sembradas con suplementación. El uso de pasturas sembradas y la suplementación otoñoinvernal
permitiría aumentar la capacidad de carga del sub-sistema recriador y, además, mejorar el desempeño individual de los animales (mejor balance energía–proteína). La suplementación en estas condiciones tendría un efecto aditivo-sustitutivo sobre la pastura. |
Palabras claves : |
ANIMAL PRODUCTION; BASALT; LAMB. |
Thesagro : |
BASALTO; PRODUCCIÓN ANIMAL; TERNEROS. |
Asunto categoría : |
L01 Ganadería |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/10748/1/REVISTA-INIA-53p18-21.pdf
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Marc : |
LEADER 01212naa a2200229 a 4500 001 1022394 005 2018-07-10 008 2018 bl uuuu u00u1 u #d 022 $a1510-9011 100 1 $aLUZARDO, S. 245 $aEl uso de pasturas sembradas y suplementación en la recría de terneros machos en el Basalto. 260 $c2018 490 $a(Revista INIA; 53) 520 $aExisten diferentes estrategias de intensificación de la recría, como por ejemplo el creep feeding, el pastoreo de campo natural diferido, la suplementación estratégica sobre campo natural, y tal vez lo más intensivo, el uso de pasturas sembradas con suplementación. El uso de pasturas sembradas y la suplementación otoñoinvernal permitiría aumentar la capacidad de carga del sub-sistema recriador y, además, mejorar el desempeño individual de los animales (mejor balance energía–proteína). La suplementación en estas condiciones tendría un efecto aditivo-sustitutivo sobre la pastura. 650 $aBASALTO 650 $aPRODUCCIÓN ANIMAL 650 $aTERNEROS 653 $aANIMAL PRODUCTION 653 $aBASALT 653 $aLAMB 700 1 $aCUADRO, R. 773 $tRevista INIA Uruguay, 2018, no. 53, p. 18-21.
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