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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
29/09/2014 |
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Actualizado : |
24/06/2021 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
FEDERICI, M.; MARCONDES, J.A.; PICCHI, S.C.; STUCHI, E.S.; FADEL, A.L.; LAIA, M.L.; LEMOS, M.V.F. |
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Afiliación : |
MARIA TERESA FEDERICI RODRIGUEZ, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. |
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Título : |
Xylella fastidiosa: An in vivo system to study possible survival strategies within citrus xylem vessels based on global gene expression analysis. |
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Fecha de publicación : |
2012 |
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Fuente / Imprenta : |
Electronic Journal of Biotechnology, 2012, v.15, no.3, p.1-33. |
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ISSN : |
0717-3458 |
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DOI : |
http://dx.doi.org/10.2225/vol15-issue3-fulltext-4 |
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Idioma : |
Inglés |
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Contenido : |
ABSTRACT.
Xylella fastidiosa inhabits the plant xylem, a nutrient-poor environment, so that mechanisms to sense and respond to adverse environmental conditions are extremely important for bacterial survival in the plant host. Although the complete genome sequences of different Xylella strains have been determined, little is known about stress responses and gene regulation in these organisms. In this work, a DNA microarray was constructed containing 2,600 ORFs identified in the genome sequencing project of Xylella fastidiosa 9a5c strain, and used to check global gene expression differences in the bacteria when it is infecting a symptomatic and a tolerant citrus tree. Different patterns of expression were found in each variety, suggesting that bacteria are responding differentially according to each plant xylem environment. The global gene expression profile was determined and several genes related to bacterial survival in stressed conditions were found to be differentially expressed between varieties, suggesting the involvement of different strategies for adaptation to the environment. The expression pattern of some genes related to the heat shock response, toxin and detoxification processes, adaptation to atypical conditions, repair systems as well as some regulatory genes are discussed in this paper. DNA microarray proved to be a powerful technique for global transcriptome analyses. This is one of the first studies of Xylella fastidiosa gene expression in vivo which helped to increase insight into stress responses and possible bacterial survival mechanisms in the nutrient-poor environment of xylem vessels. MenosABSTRACT.
Xylella fastidiosa inhabits the plant xylem, a nutrient-poor environment, so that mechanisms to sense and respond to adverse environmental conditions are extremely important for bacterial survival in the plant host. Although the complete genome sequences of different Xylella strains have been determined, little is known about stress responses and gene regulation in these organisms. In this work, a DNA microarray was constructed containing 2,600 ORFs identified in the genome sequencing project of Xylella fastidiosa 9a5c strain, and used to check global gene expression differences in the bacteria when it is infecting a symptomatic and a tolerant citrus tree. Different patterns of expression were found in each variety, suggesting that bacteria are responding differentially according to each plant xylem environment. The global gene expression profile was determined and several genes related to bacterial survival in stressed conditions were found to be differentially expressed between varieties, suggesting the involvement of different strategies for adaptation to the environment. The expression pattern of some genes related to the heat shock response, toxin and detoxification processes, adaptation to atypical conditions, repair systems as well as some regulatory genes are discussed in this paper. DNA microarray proved to be a powerful technique for global transcriptome analyses. This is one of the first studies of Xylella fastidiosa gene expression in vivo which helped... Presentar Todo |
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Thesagro : |
CITRUS; CYDIA. |
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Asunto categoría : |
F01 Cultivo |
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Marc : |
LEADER 02405naa a2200241 a 4500
001 1050705
005 2021-06-24
008 2012 bl uuuu u00u1 u #d
022 $a0717-3458
024 7 $ahttp://dx.doi.org/10.2225/vol15-issue3-fulltext-4$2DOI
100 1 $aFEDERICI, M.
245 $aXylella fastidiosa$bAn in vivo system to study possible survival strategies within citrus xylem vessels based on global gene expression analysis.$h[electronic resource]
260 $c2012
520 $aABSTRACT. Xylella fastidiosa inhabits the plant xylem, a nutrient-poor environment, so that mechanisms to sense and respond to adverse environmental conditions are extremely important for bacterial survival in the plant host. Although the complete genome sequences of different Xylella strains have been determined, little is known about stress responses and gene regulation in these organisms. In this work, a DNA microarray was constructed containing 2,600 ORFs identified in the genome sequencing project of Xylella fastidiosa 9a5c strain, and used to check global gene expression differences in the bacteria when it is infecting a symptomatic and a tolerant citrus tree. Different patterns of expression were found in each variety, suggesting that bacteria are responding differentially according to each plant xylem environment. The global gene expression profile was determined and several genes related to bacterial survival in stressed conditions were found to be differentially expressed between varieties, suggesting the involvement of different strategies for adaptation to the environment. The expression pattern of some genes related to the heat shock response, toxin and detoxification processes, adaptation to atypical conditions, repair systems as well as some regulatory genes are discussed in this paper. DNA microarray proved to be a powerful technique for global transcriptome analyses. This is one of the first studies of Xylella fastidiosa gene expression in vivo which helped to increase insight into stress responses and possible bacterial survival mechanisms in the nutrient-poor environment of xylem vessels.
650 $aCITRUS
650 $aCYDIA
700 1 $aMARCONDES, J.A.
700 1 $aPICCHI, S.C.
700 1 $aSTUCHI, E.S.
700 1 $aFADEL, A.L.
700 1 $aLAIA, M.L.
700 1 $aLEMOS, M.V.F.
773 $tElectronic Journal of Biotechnology, 2012$gv.15, no.3, p.1-33.
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Registro original : |
INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA Tacuarembó. |
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Fecha actual : |
21/02/2014 |
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Actualizado : |
22/03/2019 |
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Tipo de producción científica : |
Documentos |
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Autor : |
LUZARDO, S.; CUADRO, R.; BOVE, P.; BOCKING, B.; MONTOSSI, F.; LAVECCHIA, A. |
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Afiliación : |
SANTIAGO FELIPE LUZARDO VILLAR, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; WASHINGTON ROBIN CUADRO LOPEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FABIO MARCELO MONTOSSI PORCHILE, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ANDRES PASCUAL LAVECCHIA GONZALEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
2007. I. Experiencia de campo en la recría de terneros Hereford sobre un verdeo de raigrás (CV. LE 284) implantado sobre un rastrojo de arroz. II. Efecto de la utilización de dos cargas en la recría de terneros Hereford sobre un verdeo de raigrás (CV. LE 284) implantado sobre un rastrojo de arroz. |
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Fecha de publicación : |
2008 |
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Fuente / Imprenta : |
In: INIA TACUAREMBÓ. Presentación resultados experimentales arroz Zafra 2007-2008. Tacuarembó (Uruguay): INIA, 2008. |
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Páginas : |
p. 61-69 |
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Serie : |
(INIA Serie Actividades de Difusión ; 543) |
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Idioma : |
Español |
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Contenido : |
I)Objetivo General: Evaluar la productividad del uso de un verdeo de raigrás sembrado sobre rastrojo de arroz, para mejorar el proceso de recría de terneros/as Hereford, en el marco de la intensificación productiva de un sistema de rotación arroz ? pasturas de la región norte del Uruguay. II) ?Objetivo General: Evaluar la productividad de un verdeo de raigrás, utilizado para el proceso de recría de terneros Hereford, bajo el manejo comparativo de dos cargas animales, en el marco de la intensificación de un sistema de rotación arroz ? pasturas de la región Norte del Uruguay. |
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Palabras claves : |
RICE (ORYZA SATIVA L.). |
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Thesagro : |
ARROZ; PRODUCCION ANIMAL. |
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Asunto categoría : |
F01 Cultivo |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/9205/1/SAD-543p61-69.pdf
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Marc : |
LEADER 01553naa a2200241 a 4500
001 1021034
005 2019-03-22
008 2008 bl uuuu u00u1 u #d
100 1 $aLUZARDO, S.
245 $a2007. I. Experiencia de campo en la recría de terneros Hereford sobre un verdeo de raigrás (CV. LE 284) implantado sobre un rastrojo de arroz. II. Efecto de la utilización de dos cargas en la recría de terneros Hereford sobre un verdeo de raigrás (CV. LE 284) implantado sobre un rastrojo de arroz.
260 $c2008
300 $ap. 61-69
490 $a(INIA Serie Actividades de Difusión ; 543)
520 $aI)Objetivo General: Evaluar la productividad del uso de un verdeo de raigrás sembrado sobre rastrojo de arroz, para mejorar el proceso de recría de terneros/as Hereford, en el marco de la intensificación productiva de un sistema de rotación arroz ? pasturas de la región norte del Uruguay. II) ?Objetivo General: Evaluar la productividad de un verdeo de raigrás, utilizado para el proceso de recría de terneros Hereford, bajo el manejo comparativo de dos cargas animales, en el marco de la intensificación de un sistema de rotación arroz ? pasturas de la región Norte del Uruguay.
650 $aARROZ
650 $aPRODUCCION ANIMAL
653 $aRICE (ORYZA SATIVA L.)
700 1 $aCUADRO, R.
700 1 $aBOVE, P.
700 1 $aBOCKING, B.
700 1 $aMONTOSSI, F.
700 1 $aLAVECCHIA, A.
773 $tIn: INIA TACUAREMBÓ. Presentación resultados experimentales arroz Zafra 2007-2008. Tacuarembó (Uruguay): INIA, 2008.
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INIA Tacuarembó (TBO) |
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