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 | Acceso al texto completo restringido a Biblioteca INIA Tacuarembó. Por información adicional contacte bibliotb@tb.inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Tacuarembó; INIA Treinta y Tres. |
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Fecha : |
18/10/2018 |
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Actualizado : |
09/10/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
MARESCA, S.; LÓPEZ VALIENTE, S.; RODRÍGUEZ, A.M.; LONG, N.M.; PAVAN, E.; QUINTANS, G. |
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Afiliación : |
S. MARESCA, Cuenca del Salado Experimental Station, National Institute of Agricultural Technology, Buenos Aires.; S. LÓPEZ VALIENTE, Cuenca del Salado Experimental Station, National Institute of Agricultural Technology, Buenos Aires.; A.M. RODRÍGUEZ, Cuenca del Salado Experimental Station, National Institute of Agricultural Technology, Buenos Aires.; N.M. LONG, Department of Animal Veterinary Sciences, Clemson University, USA.; E. PAVAN, Cuenca del Salado Experimental Station, National Institute of Agricultural Technology, Buenos Aires.; GRACIELA QUINTANS ILARIA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Effect of protein restriction of bovine dams during late gestation on offspring postnatal growth, glucose-insulin metabolism and IGF-1 consentration. |
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Fecha de publicación : |
2018 |
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Fuente / Imprenta : |
Livestock Science, 2018, 212: 120-126. |
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DOI : |
10.1016/j.livsci.2018.04.009 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 10 October 2017. Received in revised from 27 March 2018, accepted 11 April 2018. |
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Contenido : |
Abstract:
The objective of this study was determine whether the amount of protein provided to cows during late gestation would affect postnatal growth and lead to changes on glucose, insulin and insulin-like growth factor concentrations. At 134 d prepartum, 68 multiparous Angus cows were blocked by BW and expected calving date and
randomly assigned to low protein level (LP, 6% CP) or high protein level (HP, 12% CP) and were allotted in 12 pens per treatment. At calving, cows were managed together on improved pastures until weaning. Calves were weighed and body measurements were recorded at birth. Blood samples were taken at birth and each 30 d until weaning at 180 d of age. Body weight at birth on HP progeny tended to be great than LP progeny (P=0.06). At birth, calves from LP dams had greater head circumference (P ? 0.01), heart girth (P=0.01) and cannon bone circumference (P=0.02). However, head circumference /birth BW ratio (P=0.04), heart girth /birth BW ratio (P=0.01), body length /birth BW ratio (P=0.05) and height /birth BW ratio (P=0.01) was greater on LP calves. Body mass index was greater in HP calves (P=0.04). No differences were found on BW of calves at weaning, adjusted 205d BW and ADG during lactation (P ? 0.10). Glucose concentrations were greater on LP
calves than HP calves from birth to weaning (81.0 ± 1.5 vs. 76.4 ± 1.2 mg/dl; P ? 0.001) without any change in insulin concentrations during preweaning growth (LP=1.61 ± 0.04 ng/ml; HP=1.61 ± 0.04 ng/ml; P ? 0.10). Insulin-like growth factor concentrations was less on LP calves at birth (P ? 0.05) and similar to HP calves
during postnatal growth (P ? 0.10). These data demonstrate that low protein during late gestation in bovine dams may affect fetal growth with proportional increasing of head circumference and low body mass index. Level of protein compromised glucose regulation in early life and affected the IGF-I concentration of newborn calves. MenosAbstract:
The objective of this study was determine whether the amount of protein provided to cows during late gestation would affect postnatal growth and lead to changes on glucose, insulin and insulin-like growth factor concentrations. At 134 d prepartum, 68 multiparous Angus cows were blocked by BW and expected calving date and
randomly assigned to low protein level (LP, 6% CP) or high protein level (HP, 12% CP) and were allotted in 12 pens per treatment. At calving, cows were managed together on improved pastures until weaning. Calves were weighed and body measurements were recorded at birth. Blood samples were taken at birth and each 30 d until weaning at 180 d of age. Body weight at birth on HP progeny tended to be great than LP progeny (P=0.06). At birth, calves from LP dams had greater head circumference (P ? 0.01), heart girth (P=0.01) and cannon bone circumference (P=0.02). However, head circumference /birth BW ratio (P=0.04), heart girth /birth BW ratio (P=0.01), body length /birth BW ratio (P=0.05) and height /birth BW ratio (P=0.01) was greater on LP calves. Body mass index was greater in HP calves (P=0.04). No differences were found on BW of calves at weaning, adjusted 205d BW and ADG during lactation (P ? 0.10). Glucose concentrations were greater on LP
calves than HP calves from birth to weaning (81.0 ± 1.5 vs. 76.4 ± 1.2 mg/dl; P ? 0.001) without any change in insulin concentrations during preweaning growth (LP=1.61 ± 0.04 ng/ml; HP=1.61 ± 0.04 ng/ml; P ? 0.... Presentar Todo |
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Palabras claves : |
GESTATION; GLUCOSE METABOLISM; IGF-1; POSTNATAL GROWTH; UNDERNUTRITION. |
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Thesagro : |
BOVINOS; GESTACION. |
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Asunto categoría : |
L10 Genética y mejoramiento animal |
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Marc : |
LEADER 02900naa a2200289 a 4500
001 1059652
005 2019-10-09
008 2018 bl uuuu u00u1 u #d
024 7 $a10.1016/j.livsci.2018.04.009$2DOI
100 1 $aMARESCA, S.
245 $aEffect of protein restriction of bovine dams during late gestation on offspring postnatal growth, glucose-insulin metabolism and IGF-1 consentration.$h[electronic resource]
260 $c2018
500 $aArticle history: Received 10 October 2017. Received in revised from 27 March 2018, accepted 11 April 2018.
520 $aAbstract: The objective of this study was determine whether the amount of protein provided to cows during late gestation would affect postnatal growth and lead to changes on glucose, insulin and insulin-like growth factor concentrations. At 134 d prepartum, 68 multiparous Angus cows were blocked by BW and expected calving date and randomly assigned to low protein level (LP, 6% CP) or high protein level (HP, 12% CP) and were allotted in 12 pens per treatment. At calving, cows were managed together on improved pastures until weaning. Calves were weighed and body measurements were recorded at birth. Blood samples were taken at birth and each 30 d until weaning at 180 d of age. Body weight at birth on HP progeny tended to be great than LP progeny (P=0.06). At birth, calves from LP dams had greater head circumference (P ? 0.01), heart girth (P=0.01) and cannon bone circumference (P=0.02). However, head circumference /birth BW ratio (P=0.04), heart girth /birth BW ratio (P=0.01), body length /birth BW ratio (P=0.05) and height /birth BW ratio (P=0.01) was greater on LP calves. Body mass index was greater in HP calves (P=0.04). No differences were found on BW of calves at weaning, adjusted 205d BW and ADG during lactation (P ? 0.10). Glucose concentrations were greater on LP calves than HP calves from birth to weaning (81.0 ± 1.5 vs. 76.4 ± 1.2 mg/dl; P ? 0.001) without any change in insulin concentrations during preweaning growth (LP=1.61 ± 0.04 ng/ml; HP=1.61 ± 0.04 ng/ml; P ? 0.10). Insulin-like growth factor concentrations was less on LP calves at birth (P ? 0.05) and similar to HP calves during postnatal growth (P ? 0.10). These data demonstrate that low protein during late gestation in bovine dams may affect fetal growth with proportional increasing of head circumference and low body mass index. Level of protein compromised glucose regulation in early life and affected the IGF-I concentration of newborn calves.
650 $aBOVINOS
650 $aGESTACION
653 $aGESTATION
653 $aGLUCOSE METABOLISM
653 $aIGF-1
653 $aPOSTNATAL GROWTH
653 $aUNDERNUTRITION
700 1 $aLÓPEZ VALIENTE, S.
700 1 $aRODRÍGUEZ, A.M.
700 1 $aLONG, N.M.
700 1 $aPAVAN, E.
700 1 $aQUINTANS, G.
773 $tLivestock Science, 2018, 212: 120-126.
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Registro original : |
INIA Tacuarembó (TBO) |
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Biblioteca
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Clasificación
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
17/08/2023 |
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Actualizado : |
17/08/2023 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
FERNÁNDEZ, M.; CASAUX, M.L.; FRAGA, M.; VIGNOLI, R.; BADO, I.; ZUNINO, P.; UMPIÉRREZ, A. |
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Afiliación : |
MAGALÍ FERNÁNDEZ, Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable, Avenida Italia 3318, Montevideo 11600, Uruguay; MARÍA LAURA CASAUX, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARTIN FRAGA COTELO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RAFAEL VIGNOLI, Departamento de Bacteriología y Virología, Facultad de Medicina, UdelaR, Av Alfredo Navarro 3051, Montevideo 11600, Uruguay; INÉS BADO, Departamento de Bacteriología y Virología, Facultad de Medicina, UdelaR, Av Alfredo Navarro 3051, Montevideo 11600, Uruguay; PABLO ZUNINO, Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable, Avenida Italia 3318, Montevideo 11600, Uruguay; ANA UMPIÉRREZ, Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable, Avenida Italia 3318, Montevideo 11600, Uruguay. |
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Título : |
Shiga toxin-producing Escherichia coli (STEC) associated with calf mortality in Uruguay. |
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Fecha de publicación : |
2023 |
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Fuente / Imprenta : |
Microorganisms. 2023, Volume 11, Issue 7, p. 1704. https://doi.org/10.3390/microorganisms11071704 ---- OPEN ACCESS. |
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ISSN : |
2076-2607 (electronic). |
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DOI : |
10.3390/microorganisms11071704 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 17 May 2023; Revised 5 June 2023; Accepted 8 June 2023; Published 29 June 2023. -- Academic Editors: Kim Stanford,
Flemming Scheutz, Chad R. Laing, Linda Chui, Nicole Van De Kar, Patricia Griffin, Tim A. McAllister and Gillian Tarr. -- This article belongs to the Special Issue The Science of Shiga Toxin-Producing (Verotoxin-Producing) Escherichia coli (STEC): An Ongoing One Health Journey toward Improved Health and Food Safety) (https://www.mdpi.com/journal/microorganisms/special_issues/STEC_VTEC ) -- LICENSE: Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). -- FUNDING: This research was partially funded by the project PL-15 from "Instituto Nacional de Investigación Agropecuaria" (INIA).. -- Supplementary imaterials an be downloaded at: https://www.mdpi.com/article/10.3390/microorganisms11071704/s1. Table S1: Origin of the animals. Table S2: Virulence profiles in E. coli isolates recovered from dead animals (n = 221). |
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Contenido : |
ABSTRACT.- In Uruguay, the mortality of dairy calves due to infectious diseases is high. Escherichia coli is a natural inhabitant of the intestinal microbiota, but can cause several infections. The aim of the work was to characterize E. coli isolates from intestinal and extraintestinal origin of dead newborn calves. Using PCR, virulence gene characteristics of pathogenic E. coli were searched. The pathogenic E. coli were molecularly characterized and the phylogroup, serogroup and the Stx subtype were determined. Antibiotic susceptibility was determined using the Kirby-Bauer disk diffusion method and plasmid-mediated quinolone resistance (PMQR) genes with PCR. Finally, clonal relationships were inferred using PFGE. Gene characteristics of the Shiga toxin-producing E. coli (STEC), Enteropathogenic E. coli (EPEC) and Necrotoxigenic E. coli (NTEC) were identified. The prevalence of the iucD, afa8E, f17, papC, stx1, eae and ehxA genes was high and no f5, f41, saa, sfaDE, cdtIV, lt, sta or stx2 were detected. The prevalence of STEC gene stx1 in the dead calves stood out and was higher compared with previous studies conducted in live calves, and STEC LEE+ (Enterohemorrhagic E. coli (EHEC)) isolates with stx1/eae/ehxA genotypes were more frequently identified in the intestinal than in the extraintestinal environment. E. coli isolates were assigned to phylogroups A, B1, D and E, and some belonged to the O111 serogroup. stx1a and stx1c subtypes were determined in STEC. A high prevalence of multi-resistance among STEC and qnrB genes was determined. The PFGE showed a high diversity of pathogenic strains with similar genetic profiles. It can be speculated that EHEC (stx1/eae/ehxA) could play an important role in mortality. The afa8E, f17G1 and papC genes could also have a role in calf mortality. Multidrug resistance defies disease treatment and increases the risk of death, while the potential transmissibility of genes to other species constitutes a threat to public health. © 2023 by the authors. Licensee MDPI, Basel, Switzerland. MenosABSTRACT.- In Uruguay, the mortality of dairy calves due to infectious diseases is high. Escherichia coli is a natural inhabitant of the intestinal microbiota, but can cause several infections. The aim of the work was to characterize E. coli isolates from intestinal and extraintestinal origin of dead newborn calves. Using PCR, virulence gene characteristics of pathogenic E. coli were searched. The pathogenic E. coli were molecularly characterized and the phylogroup, serogroup and the Stx subtype were determined. Antibiotic susceptibility was determined using the Kirby-Bauer disk diffusion method and plasmid-mediated quinolone resistance (PMQR) genes with PCR. Finally, clonal relationships were inferred using PFGE. Gene characteristics of the Shiga toxin-producing E. coli (STEC), Enteropathogenic E. coli (EPEC) and Necrotoxigenic E. coli (NTEC) were identified. The prevalence of the iucD, afa8E, f17, papC, stx1, eae and ehxA genes was high and no f5, f41, saa, sfaDE, cdtIV, lt, sta or stx2 were detected. The prevalence of STEC gene stx1 in the dead calves stood out and was higher compared with previous studies conducted in live calves, and STEC LEE+ (Enterohemorrhagic E. coli (EHEC)) isolates with stx1/eae/ehxA genotypes were more frequently identified in the intestinal than in the extraintestinal environment. E. coli isolates were assigned to phylogroups A, B1, D and E, and some belonged to the O111 serogroup. stx1a and stx1c subtypes were determined in STEC. A high prevalen... Presentar Todo |
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Palabras claves : |
Dairy calf; MDR STEC; Mortality; PLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL - INIA; Shiga toxin-producing E. coli. |
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Asunto categoría : |
L01 Ganadería |
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URL : |
https://www.mdpi.com/2076-2607/11/7/1704/pdf
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Marc : |
LEADER 04099naa a2200289 a 4500
001 1064289
005 2023-08-17
008 2023 bl uuuu u00u1 u #d
022 $a2076-2607 (electronic).
024 7 $a10.3390/microorganisms11071704$2DOI
100 1 $aFERNÁNDEZ, M.
245 $aShiga toxin-producing Escherichia coli (STEC) associated with calf mortality in Uruguay.$h[electronic resource]
260 $c2023
500 $aArticle history: Received 17 May 2023; Revised 5 June 2023; Accepted 8 June 2023; Published 29 June 2023. -- Academic Editors: Kim Stanford, Flemming Scheutz, Chad R. Laing, Linda Chui, Nicole Van De Kar, Patricia Griffin, Tim A. McAllister and Gillian Tarr. -- This article belongs to the Special Issue The Science of Shiga Toxin-Producing (Verotoxin-Producing) Escherichia coli (STEC): An Ongoing One Health Journey toward Improved Health and Food Safety) (https://www.mdpi.com/journal/microorganisms/special_issues/STEC_VTEC ) -- LICENSE: Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). -- FUNDING: This research was partially funded by the project PL-15 from "Instituto Nacional de Investigación Agropecuaria" (INIA).. -- Supplementary imaterials an be downloaded at: https://www.mdpi.com/article/10.3390/microorganisms11071704/s1. Table S1: Origin of the animals. Table S2: Virulence profiles in E. coli isolates recovered from dead animals (n = 221).
520 $aABSTRACT.- In Uruguay, the mortality of dairy calves due to infectious diseases is high. Escherichia coli is a natural inhabitant of the intestinal microbiota, but can cause several infections. The aim of the work was to characterize E. coli isolates from intestinal and extraintestinal origin of dead newborn calves. Using PCR, virulence gene characteristics of pathogenic E. coli were searched. The pathogenic E. coli were molecularly characterized and the phylogroup, serogroup and the Stx subtype were determined. Antibiotic susceptibility was determined using the Kirby-Bauer disk diffusion method and plasmid-mediated quinolone resistance (PMQR) genes with PCR. Finally, clonal relationships were inferred using PFGE. Gene characteristics of the Shiga toxin-producing E. coli (STEC), Enteropathogenic E. coli (EPEC) and Necrotoxigenic E. coli (NTEC) were identified. The prevalence of the iucD, afa8E, f17, papC, stx1, eae and ehxA genes was high and no f5, f41, saa, sfaDE, cdtIV, lt, sta or stx2 were detected. The prevalence of STEC gene stx1 in the dead calves stood out and was higher compared with previous studies conducted in live calves, and STEC LEE+ (Enterohemorrhagic E. coli (EHEC)) isolates with stx1/eae/ehxA genotypes were more frequently identified in the intestinal than in the extraintestinal environment. E. coli isolates were assigned to phylogroups A, B1, D and E, and some belonged to the O111 serogroup. stx1a and stx1c subtypes were determined in STEC. A high prevalence of multi-resistance among STEC and qnrB genes was determined. The PFGE showed a high diversity of pathogenic strains with similar genetic profiles. It can be speculated that EHEC (stx1/eae/ehxA) could play an important role in mortality. The afa8E, f17G1 and papC genes could also have a role in calf mortality. Multidrug resistance defies disease treatment and increases the risk of death, while the potential transmissibility of genes to other species constitutes a threat to public health. © 2023 by the authors. Licensee MDPI, Basel, Switzerland.
653 $aDairy calf
653 $aMDR STEC
653 $aMortality
653 $aPLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL - INIA
653 $aShiga toxin-producing E. coli
700 1 $aCASAUX, M.L.
700 1 $aFRAGA, M.
700 1 $aVIGNOLI, R.
700 1 $aBADO, I.
700 1 $aZUNINO, P.
700 1 $aUMPIÉRREZ, A.
773 $tMicroorganisms. 2023, Volume 11, Issue 7, p. 1704. https://doi.org/10.3390/microorganisms11071704 ---- OPEN ACCESS.
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