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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
25/05/2023 |
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Actualizado : |
25/05/2023 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
GARAYCOCHEA, S.; ALTIER, N.; LEONI, C.; NEAL, A.L.; ROMERO, H. |
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Afiliación : |
SILVIA RAQUEL GARAYCOCHEA SOLSONA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; NORA ADRIANA ALTIER MANZINI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CAROLINA LEONI VELAZCO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ANDREW L. NEAL, Net-Zero and Resilient Farming, Rothamsted Research, North Wyke, Okehampton, United Kingdom; HÉCTOR ROMERO, Laboratorio de Organización y Evolución del Genoma/Genómica Evolutiva, Departamento de Ecolog??a y Evolución, Facultad de Ciencias/CURE, Universidad de la República, Maldonado, Uruguay. |
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Título : |
Abundance and phylogenetic distribution of eight key enzymes of the phosphorus biogeochemical cycle in grassland soils. |
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Complemento del título : |
Research article. |
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Fecha de publicación : |
2023 |
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Fuente / Imprenta : |
Environmental Microbiology Reports, 2023. https://doi.org/10.1111/1758-2229.13159 --OPEN ACCESS. [Article in Press] |
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ISSN : |
1758-2229 |
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DOI : |
10.1111/1758-2229.13159 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 28 December 2022; Accepted 12 April 2023. -- Correspondence: Héctor Romero, Laboratorio de Organización y Evolución del Genoma/Genómica Evolutiva, Departamento de Ecología y Evolución, Facultad de Ciencias/CURE, Universidad de la República, Maldonado, Uruguay. Email: eletor@fcien.edu.uy ; Silvia Garaycochea, Instituto Nacional de Investigacion Agropecuaria (INIA), Estación Experimental INIA Las Brujas, Ruta 48 Km 10, Canelones, 90200, Uruguay. Email: sgaraycochea@inia.org.uy -- Funding: This work was funded by the Instituto Nacional de Investigación Agropecuaria, INIA (Project SA47, SA 26 and SA 24), Agencia Nacional de Investigación e Innovación (POS NAC 2015 1 110075). -- License: This is an open access article under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ ) -- Supporting information: Additional supporting information can be found online
in the Supporting Information section at the end of this article. |
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Contenido : |
Grassland biomes provide valuable ecosystem services, including nutrient cycling. Organic phosphorus (Po) represents more than half of the total P in soils. Soil microorganisms release organic P through enzymatic processes, with alkaline phosphatases, acid phosphatases and phytases being the key P enzymes involved in the cycling of organic P. This study analysed 74 soil metagenomes from 17 different grassland biomes worldwide to evaluate the distribution and abundance of eight key P enzymes (PhoD, PhoX, PhoA, Nsap-A, Nsap-B, Nsap-C, BPP and CPhy) and their relationship with environmental factors. Our analyses showed that alkaline phosphatase phoD was the dataset's most abundant P-enzyme encoding genes, with a wide phylogenetic distribution. Followed by the acid phosphatases Nsap-A and Nsap-C showed similar abundance but a different distribution in their respective phylogenetic trees. Multivariate analyses revealed that pH, Tmax, SOC and soil moisture were associated with the abundance and diversity of all genes studied. PhoD and phoX genes strongly correlated with SOC and clay, and the phoX gene was more common in soils with low to medium SOC and neutral pH. In particular, P-enzyme genes tended to respond in a positively correlated manner among them, suggesting a complex relationship of abundance and diversity among them. © 2023 The Authors. Environmental Microbiology Reports published by Applied Microbiology International and John Wiley & Sons Ltd. |
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Palabras claves : |
Biomass; Biomes; Ecosystem; Grasslands; Statistical analyses. |
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Asunto categoría : |
P01 Conservación de la naturaleza y recursos de La tierra |
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URL : |
https://ami-journals.onlinelibrary.wiley.com/doi/pdf/10.1111/1758-2229.13159
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Marc : |
LEADER 03302naa a2200265 a 4500
001 1064166
005 2023-05-25
008 2023 bl uuuu u00u1 u #d
022 $a1758-2229
024 7 $a10.1111/1758-2229.13159$2DOI
100 1 $aGARAYCOCHEA, S.
245 $aAbundance and phylogenetic distribution of eight key enzymes of the phosphorus biogeochemical cycle in grassland soils.$h[electronic resource]
260 $c2023
500 $aArticle history: Received 28 December 2022; Accepted 12 April 2023. -- Correspondence: Héctor Romero, Laboratorio de Organización y Evolución del Genoma/Genómica Evolutiva, Departamento de Ecología y Evolución, Facultad de Ciencias/CURE, Universidad de la República, Maldonado, Uruguay. Email: eletor@fcien.edu.uy ; Silvia Garaycochea, Instituto Nacional de Investigacion Agropecuaria (INIA), Estación Experimental INIA Las Brujas, Ruta 48 Km 10, Canelones, 90200, Uruguay. Email: sgaraycochea@inia.org.uy -- Funding: This work was funded by the Instituto Nacional de Investigación Agropecuaria, INIA (Project SA47, SA 26 and SA 24), Agencia Nacional de Investigación e Innovación (POS NAC 2015 1 110075). -- License: This is an open access article under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ ) -- Supporting information: Additional supporting information can be found online in the Supporting Information section at the end of this article.
520 $aGrassland biomes provide valuable ecosystem services, including nutrient cycling. Organic phosphorus (Po) represents more than half of the total P in soils. Soil microorganisms release organic P through enzymatic processes, with alkaline phosphatases, acid phosphatases and phytases being the key P enzymes involved in the cycling of organic P. This study analysed 74 soil metagenomes from 17 different grassland biomes worldwide to evaluate the distribution and abundance of eight key P enzymes (PhoD, PhoX, PhoA, Nsap-A, Nsap-B, Nsap-C, BPP and CPhy) and their relationship with environmental factors. Our analyses showed that alkaline phosphatase phoD was the dataset's most abundant P-enzyme encoding genes, with a wide phylogenetic distribution. Followed by the acid phosphatases Nsap-A and Nsap-C showed similar abundance but a different distribution in their respective phylogenetic trees. Multivariate analyses revealed that pH, Tmax, SOC and soil moisture were associated with the abundance and diversity of all genes studied. PhoD and phoX genes strongly correlated with SOC and clay, and the phoX gene was more common in soils with low to medium SOC and neutral pH. In particular, P-enzyme genes tended to respond in a positively correlated manner among them, suggesting a complex relationship of abundance and diversity among them. © 2023 The Authors. Environmental Microbiology Reports published by Applied Microbiology International and John Wiley & Sons Ltd.
653 $aBiomass
653 $aBiomes
653 $aEcosystem
653 $aGrasslands
653 $aStatistical analyses
700 1 $aALTIER, N.
700 1 $aLEONI, C.
700 1 $aNEAL, A.L.
700 1 $aROMERO, H.
773 $tEnvironmental Microbiology Reports, 2023. https://doi.org/10.1111/1758-2229.13159 --OPEN ACCESS. [Article in Press]
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INIA Las Brujas (LB) |
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 | Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha actual : |
18/12/2020 |
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Actualizado : |
18/12/2020 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
COPPOLA, N.; FREIRE, B.; UMPIÉRREZ, A.; CORDEIRO, N.F.; AVILA. P.; TRENCHI, G.; CASTRO, G.; CASAUX, M.L.; FRAGA, M.; ZUNINO, P.; BADO, I.; VIGNOLI, R. |
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Afiliación : |
NADIA COPPOLA, Departamento de Bacteriología y Virología, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay.; BIBIANA FREIRE, Unidad Académica Animales de Granja, Facultad de Veterinaria, Universidad de la República, Montevideo, Uruguay.; ANA UMPIÉRREZ, Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay.; NICOLÁS F. CORDEIRO, Departamento de Bacteriología y Virología, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay.; PABLO AVILA, Departamento de Bacteriología y Virología, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay.; GUSTAVO TRENCHI, Veterinario de Libre Ejercicio, Montevideo, Uruguay.; GUSTAVO CASTRO, Unidad Académica Animales de Granja, Facultad de Veterinaria, Universidad de la República, Montevideo, Uruguay.; MARÍA LAURA CASAUX, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARTIN FRAGA COTELO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; PABLO ZUNINO, Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay.; INÉS BADO, Departamento de Bacteriología y Virología, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay; RAFAEL VIGNOLI, Departamento de Bacteriología y Virología, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay. |
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Título : |
Transferable Resistance to Highest Priority Critically Important Antibiotics for Human Health in Escherichia coli Strains Obtained From Livestock Feces in Uruguay. (Brief Research Report Article). |
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Fecha de publicación : |
2020 |
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Fuente / Imprenta : |
Frontiers in Veterinary Science, 19 November 2020, Volume 7, Article number 588919. OPEN ACCESS. Doi: https://doi.org/10.3389/fvets.2020.588919 |
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DOI : |
10.3389/fvets.2020.588919 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: 29 July 2020/ Accepted: 19 October 2020/Published: 19 November 2020. Correspondence: Rafael Vignoli, rvignoli@higiene.edu.uy; Inés Bado, ibado@higiene.edu.uy |
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Contenido : |
Abstract: The aim of this work was to detect Escherichia coli isolates displaying resistance to oxyimino-cephalosporins, quinolones, and colistin in feces from livestock in Uruguay. During 2016?2019, fecal samples from 132 broiler and layer chicken flocks, 100 calves, and 50 pigs, were studied in Uruguay. Samples were cultured on MacConkey Agar plates supplemented with ciprofloxacin, ceftriaxone, or colistin. E. coli isolates were identified by mass spectrometry and antibiotic susceptibility testing was performed by disk diffusion agar method and colistin agar test. Antibiotic resistance genes were detected by polymerase chain reaction and sequencing. The most frequently detected resistance gene was qnrB19, recovered from 87 animals. Regarding plasmid-mediated quinolone resistance genes, qnrS1 was the second in prevalence (23 animals) followed by qnrE1, found in 6 chickens and two calves. Regarding resistance to oxyimino-cephalosporins, 8 different ?-lactamase genes were detected: blaCTX?M?8 and blaCMY?2 were found in 23 and 19 animals, respectively; next, blaCTX?M?2 and blaSHV?12 in 7 animals each, followed by blaCTX?M?14 in 5, blaCTX?M?15 and blaSHV2a in 2, and blaCTX?M?55 in a single animal. Finally, the mcr-1 gene was detected only in 8 pigs from a single farm, and in a chicken. Isolates carrying blaCMY?2 and blaSHV?12 were also found in these animals, including two isolates featuring the blaCMY?2/mcr-1 genotype. To the best of our knowledge, this is the first work in which the search for transferable resistance to highest priority critically important antibiotics for human health is carried out in chickens and pigs chains of production animals in Uruguay. MenosAbstract: The aim of this work was to detect Escherichia coli isolates displaying resistance to oxyimino-cephalosporins, quinolones, and colistin in feces from livestock in Uruguay. During 2016?2019, fecal samples from 132 broiler and layer chicken flocks, 100 calves, and 50 pigs, were studied in Uruguay. Samples were cultured on MacConkey Agar plates supplemented with ciprofloxacin, ceftriaxone, or colistin. E. coli isolates were identified by mass spectrometry and antibiotic susceptibility testing was performed by disk diffusion agar method and colistin agar test. Antibiotic resistance genes were detected by polymerase chain reaction and sequencing. The most frequently detected resistance gene was qnrB19, recovered from 87 animals. Regarding plasmid-mediated quinolone resistance genes, qnrS1 was the second in prevalence (23 animals) followed by qnrE1, found in 6 chickens and two calves. Regarding resistance to oxyimino-cephalosporins, 8 different ?-lactamase genes were detected: blaCTX?M?8 and blaCMY?2 were found in 23 and 19 animals, respectively; next, blaCTX?M?2 and blaSHV?12 in 7 animals each, followed by blaCTX?M?14 in 5, blaCTX?M?15 and blaSHV2a in 2, and blaCTX?M?55 in a single animal. Finally, the mcr-1 gene was detected only in 8 pigs from a single farm, and in a chicken. Isolates carrying blaCMY?2 and blaSHV?12 were also found in these animals, including two isolates featuring the blaCMY?2/mcr-1 genotype. To the best of our knowledge, this is the first work in wh... Presentar Todo |
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Palabras claves : |
CMY-2; CTX-M-8; E. COLI; ESBL; ESCHERICHIA COLI; MCR-1; PLATAFORMA SALUD ANIMAL; POULTRY; QNRB19; SWINE. |
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Thesagro : |
AVES DE CORRAL; CERDO. |
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Asunto categoría : |
-- |
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Marc : |
LEADER 03119nam a2200409 a 4500
001 1061590
005 2020-12-18
008 2020 bl uuuu u0uu1 u #d
024 7 $a10.3389/fvets.2020.588919$2DOI
100 1 $aCOPPOLA, N.
245 $aTransferable Resistance to Highest Priority Critically Important Antibiotics for Human Health in Escherichia coli Strains Obtained From Livestock Feces in Uruguay. (Brief Research Report Article).$h[electronic resource]
260 $aFrontiers in Veterinary Science, 19 November 2020, Volume 7, Article number 588919. OPEN ACCESS. Doi: https://doi.org/10.3389/fvets.2020.588919$c2020
500 $aArticle history: Received: 29 July 2020/ Accepted: 19 October 2020/Published: 19 November 2020. Correspondence: Rafael Vignoli, rvignoli@higiene.edu.uy; Inés Bado, ibado@higiene.edu.uy
520 $aAbstract: The aim of this work was to detect Escherichia coli isolates displaying resistance to oxyimino-cephalosporins, quinolones, and colistin in feces from livestock in Uruguay. During 2016?2019, fecal samples from 132 broiler and layer chicken flocks, 100 calves, and 50 pigs, were studied in Uruguay. Samples were cultured on MacConkey Agar plates supplemented with ciprofloxacin, ceftriaxone, or colistin. E. coli isolates were identified by mass spectrometry and antibiotic susceptibility testing was performed by disk diffusion agar method and colistin agar test. Antibiotic resistance genes were detected by polymerase chain reaction and sequencing. The most frequently detected resistance gene was qnrB19, recovered from 87 animals. Regarding plasmid-mediated quinolone resistance genes, qnrS1 was the second in prevalence (23 animals) followed by qnrE1, found in 6 chickens and two calves. Regarding resistance to oxyimino-cephalosporins, 8 different ?-lactamase genes were detected: blaCTX?M?8 and blaCMY?2 were found in 23 and 19 animals, respectively; next, blaCTX?M?2 and blaSHV?12 in 7 animals each, followed by blaCTX?M?14 in 5, blaCTX?M?15 and blaSHV2a in 2, and blaCTX?M?55 in a single animal. Finally, the mcr-1 gene was detected only in 8 pigs from a single farm, and in a chicken. Isolates carrying blaCMY?2 and blaSHV?12 were also found in these animals, including two isolates featuring the blaCMY?2/mcr-1 genotype. To the best of our knowledge, this is the first work in which the search for transferable resistance to highest priority critically important antibiotics for human health is carried out in chickens and pigs chains of production animals in Uruguay.
650 $aAVES DE CORRAL
650 $aCERDO
653 $aCMY-2
653 $aCTX-M-8
653 $aE. COLI
653 $aESBL
653 $aESCHERICHIA COLI
653 $aMCR-1
653 $aPLATAFORMA SALUD ANIMAL
653 $aPOULTRY
653 $aQNRB19
653 $aSWINE
700 1 $aFREIRE, B.
700 1 $aUMPIÉRREZ, A.
700 1 $aCORDEIRO, N.F.
700 1 $aAVILA. P.
700 1 $aTRENCHI, G.
700 1 $aCASTRO, G.
700 1 $aCASAUX, M.L.
700 1 $aFRAGA, M.
700 1 $aZUNINO, P.
700 1 $aBADO, I.
700 1 $aVIGNOLI, R.
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