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Biblioteca (s) : |
INIA La Estanzuela; INIA Las Brujas; INIA Tacuarembó; INIA Treinta y Tres. |
Fecha : |
16/10/2014 |
Actualizado : |
25/10/2019 |
Tipo de producción científica : |
Serie FPTA |
Autor : |
EASTON, C.; PAULLIER, C.; PRELIASCO, M. |
Afiliación : |
CRISTINA EASTON, MGAP/ DILAVE (División de Laboratorios de Veterinarios); CECILIA PAULLIER, MGAP/ DILAVE (División de Laboratorios de Veterinarios); MARCELA PRELIASCO, MGAP/ DILAVE (División de Laboratorios de Veterinarios). |
Título : |
Identificación de agentes infecciosos en Encefalitis de bovinos de Uruguay. |
Fecha de publicación : |
2012 |
Fuente / Imprenta : |
Montevideo (UY): INIA, 2012. |
Páginas : |
33 p. |
Serie : |
(Serie FPTA-INIA; 40) |
ISSN : |
1688-924X |
Idioma : |
Español |
Notas : |
Proyecto FPTA 274: Estudio retrospectivo para la identificación de agentes infecciosos que provocan encefalitis en bovinos. Período de Ejecución: Marzo 2009 - Agosto 2011 |
Contenido : |
Los disturbios caracterizados por sintomatología nerviosa constituyen un importante grupo de enfermedades para los bovinos. Los mismos han tenido una importancia creciente desde la aparición de la Encefalopatía Espongiforme Bovina (EEB) a mediados de la década de 1980. A partir de esto, los países exportadores de carne han tenido que realizar esfuerzos para demostrar que sus rodeos son libres de EEB. El desarrollo de este proyecto de investigación tuvo por objetivos generales: 1) Incrementar la capacidad de diagnóstico de las enfermedades nerviosas que afectan a los bovinos, poniendo especial énfasis en aquellas que son zoonosis y tienen un impacto negativo en la producción nacional. 2) Realizar la estandarización y aplicación de técnicas inmunológicas y moleculares que permitan el diagnóstico etiológico de enfermedades nerviosas en bovinos. |
Thesagro : |
BOVINOS; ENFERMEDADES INFECCIOSAS; ENFERMEDADES NERVIOSAS. |
Asunto categoría : |
-- L73 Enfermedades de los animales |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/2833/1/18429161012095245.pdf
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Marc : |
LEADER 01610nam a2200217 a 4500 001 1003507 005 2019-10-25 008 2012 bl uuuu u00u1 u #d 022 $a1688-924X 100 1 $aEASTON, C. 245 $aIdentificación de agentes infecciosos en Encefalitis de bovinos de Uruguay. 260 $aMontevideo (UY): INIA$c2012 300 $a33 p. 490 $a(Serie FPTA-INIA; 40) 500 $aProyecto FPTA 274: Estudio retrospectivo para la identificación de agentes infecciosos que provocan encefalitis en bovinos. Período de Ejecución: Marzo 2009 - Agosto 2011 520 $aLos disturbios caracterizados por sintomatología nerviosa constituyen un importante grupo de enfermedades para los bovinos. Los mismos han tenido una importancia creciente desde la aparición de la Encefalopatía Espongiforme Bovina (EEB) a mediados de la década de 1980. A partir de esto, los países exportadores de carne han tenido que realizar esfuerzos para demostrar que sus rodeos son libres de EEB. El desarrollo de este proyecto de investigación tuvo por objetivos generales: 1) Incrementar la capacidad de diagnóstico de las enfermedades nerviosas que afectan a los bovinos, poniendo especial énfasis en aquellas que son zoonosis y tienen un impacto negativo en la producción nacional. 2) Realizar la estandarización y aplicación de técnicas inmunológicas y moleculares que permitan el diagnóstico etiológico de enfermedades nerviosas en bovinos. 650 $aBOVINOS 650 $aENFERMEDADES INFECCIOSAS 650 $aENFERMEDADES NERVIOSAS 700 1 $aPAULLIER, C. 700 1 $aPRELIASCO, M.
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INIA Las Brujas (LB) |
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Biblioteca (s) : |
INIA La Estanzuela. |
Fecha actual : |
29/07/2022 |
Actualizado : |
02/09/2022 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Circulación / Nivel : |
Internacional - -- |
Autor : |
GIANNITTI, F.; ARÁOZ, V.; SILVEIRA, C.S.; FRANCIA, M.E.; ROBELLO, C.; CABRERA, A. |
Afiliación : |
FEDERICO GIANNITTI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; VIRGINIA ARÁOZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CAROLINE DA SILVA SILVEIRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARÍA E. FRANCIA, Laboratorio de Biología de Apicomplejos, Institut Pasteur de Montevideo, Montevideo, Uruguay; Departamento de Parasitología y Micología, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay.; CARLOS ROBELLO, Laboratorio de Interacciones Hospedero-Patógeno, Instituto Pasteur de Montevideo, Montevideo, Uruguay; Departamento de Bioquímica, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay.; ANDRÉS CABRERA, Laboratorio de Interacciones Hospedero-Patógeno, Instituto Pasteur de Montevideo, Montevideo, Uruguay; Unidad de Microbiología, Departamento de Patobiología, Facultad de Veterinaria, Universidad de la República, Montevideo, Uruguay. |
Título : |
A Holstein heifer infected with Neospora caninum NcUru3 congenitally transmits this strain to a viable offspring although infection does not protect her from aborting by a different N. caninum genotype in the subsequent gestation. |
Fecha de publicación : |
2022 |
Fuente / Imprenta : |
Frontiers in Veterinary Science, 2022; 9: 889157. OPEN ACCESS. Doi: https://doi.org/10.3389/fvets.2022.889157 |
DOI : |
10.3389/fvets.2022.889157 |
Idioma : |
Inglés |
Notas : |
Article history: Received 03 March 2022/Accepted 05 July 2022/Published 25 July 2022. |
Contenido : |
Abstract: Neospora caninum is a leading cause of bovine abortion worldwide. Although the genetic diversity of this apicomplexan parasite has long been recognized, there is little information on whether infection with different genotypes results in different clinical outcomes or whether infection by a given genotype impairs protective immunity against abortion induced by different genotypes. Here, we provide evidence supporting that natural subclinical infection with isolate NcUru3 of N. caninum in a pregnant heifer did not provide protection against abortion caused by a different N. caninum genotype in the subsequent gestation. A Holstein heifer delivered a healthy calf congenitally infected with N. caninum. Specific anti-N. caninum IgG was detected by indirect ELISA in sera obtained from the dam at calving and the calf before ingestion of colostrum, indicating in utero exposure to the parasite in the latter. A N. caninum strain named NcUru3 was isolated and characterized by multilocus microsatellite typing from the brain of this neonate euthanized at 9 days of age. Sixty days after calving, the cow got pregnant, although she aborted spontaneously at ~6 months of gestation. Pathologic examination of the aborted fetus and placenta revealed typical lesions of neosporosis, including encephalitis, myocarditis, hepatitis, myositis, and placentitis. Neospora caninum DNA was amplified from the fetal brain, heart, kidney, and placenta, and multilocus microsatellite typing revealed a genotype that differed from isolate NcUru3 at the level of microsatellite marker 6A (MS6A). Serum obtained from the dam at the time of abortion had IgG that cross-recognized isolate NcUru3, as demonstrated by immunoblotting, indicating that the humoral immune response did not prevent the other genotype from infecting the fetus and inducing fetoplacental lesions and abortion. This is the first description of one same dam transmitting two N. caninum genotypes to her offspring in subsequent gestations. MenosAbstract: Neospora caninum is a leading cause of bovine abortion worldwide. Although the genetic diversity of this apicomplexan parasite has long been recognized, there is little information on whether infection with different genotypes results in different clinical outcomes or whether infection by a given genotype impairs protective immunity against abortion induced by different genotypes. Here, we provide evidence supporting that natural subclinical infection with isolate NcUru3 of N. caninum in a pregnant heifer did not provide protection against abortion caused by a different N. caninum genotype in the subsequent gestation. A Holstein heifer delivered a healthy calf congenitally infected with N. caninum. Specific anti-N. caninum IgG was detected by indirect ELISA in sera obtained from the dam at calving and the calf before ingestion of colostrum, indicating in utero exposure to the parasite in the latter. A N. caninum strain named NcUru3 was isolated and characterized by multilocus microsatellite typing from the brain of this neonate euthanized at 9 days of age. Sixty days after calving, the cow got pregnant, although she aborted spontaneously at ~6 months of gestation. Pathologic examination of the aborted fetus and placenta revealed typical lesions of neosporosis, including encephalitis, myocarditis, hepatitis, myositis, and placentitis. Neospora caninum DNA was amplified from the fetal brain, heart, kidney, and placenta, and multilocus microsatellite typing revealed a... Presentar Todo |
Palabras claves : |
ABORTION; DAIRY CATTLE; GENETIC DIVERSITY; Molecular epidemiology; Multilocus microsatellite typin; Neospora caninum; PATHOLOGY; PLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL; PLATAFORMA DE SALUD ANIMAL; REPRODUCTIVE DISEASES. |
Asunto categoría : |
-- |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/16659/1/fvets-09-889157.pdf
https://www.frontiersin.org/articles/10.3389/fvets.2022.889157/full
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Marc : |
LEADER 03260naa a2200325 a 4500 001 1063470 005 2022-09-02 008 2022 bl uuuu u00u1 u #d 024 7 $a10.3389/fvets.2022.889157$2DOI 100 1 $aGIANNITTI, F. 245 $aA Holstein heifer infected with Neospora caninum NcUru3 congenitally transmits this strain to a viable offspring although infection does not protect her from aborting by a different N. caninum genotype in the subsequent gestation.$h[electronic resource] 260 $c2022 500 $aArticle history: Received 03 March 2022/Accepted 05 July 2022/Published 25 July 2022. 520 $aAbstract: Neospora caninum is a leading cause of bovine abortion worldwide. Although the genetic diversity of this apicomplexan parasite has long been recognized, there is little information on whether infection with different genotypes results in different clinical outcomes or whether infection by a given genotype impairs protective immunity against abortion induced by different genotypes. Here, we provide evidence supporting that natural subclinical infection with isolate NcUru3 of N. caninum in a pregnant heifer did not provide protection against abortion caused by a different N. caninum genotype in the subsequent gestation. A Holstein heifer delivered a healthy calf congenitally infected with N. caninum. Specific anti-N. caninum IgG was detected by indirect ELISA in sera obtained from the dam at calving and the calf before ingestion of colostrum, indicating in utero exposure to the parasite in the latter. A N. caninum strain named NcUru3 was isolated and characterized by multilocus microsatellite typing from the brain of this neonate euthanized at 9 days of age. Sixty days after calving, the cow got pregnant, although she aborted spontaneously at ~6 months of gestation. Pathologic examination of the aborted fetus and placenta revealed typical lesions of neosporosis, including encephalitis, myocarditis, hepatitis, myositis, and placentitis. Neospora caninum DNA was amplified from the fetal brain, heart, kidney, and placenta, and multilocus microsatellite typing revealed a genotype that differed from isolate NcUru3 at the level of microsatellite marker 6A (MS6A). Serum obtained from the dam at the time of abortion had IgG that cross-recognized isolate NcUru3, as demonstrated by immunoblotting, indicating that the humoral immune response did not prevent the other genotype from infecting the fetus and inducing fetoplacental lesions and abortion. This is the first description of one same dam transmitting two N. caninum genotypes to her offspring in subsequent gestations. 653 $aABORTION 653 $aDAIRY CATTLE 653 $aGENETIC DIVERSITY 653 $aMolecular epidemiology 653 $aMultilocus microsatellite typin 653 $aNeospora caninum 653 $aPATHOLOGY 653 $aPLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL 653 $aPLATAFORMA DE SALUD ANIMAL 653 $aREPRODUCTIVE DISEASES 700 1 $aARÁOZ, V. 700 1 $aSILVEIRA, C.S. 700 1 $aFRANCIA, M.E. 700 1 $aROBELLO, C. 700 1 $aCABRERA, A. 773 $tFrontiers in Veterinary Science, 2022; 9: 889157. OPEN ACCESS. Doi: https://doi.org/10.3389/fvets.2022.889157
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