| |
|
|
 | Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
|
Registro completo
|
|
Biblioteca (s) : |
INIA La Estanzuela. |
|
Fecha : |
01/10/2019 |
|
Actualizado : |
01/10/2019 |
|
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
|
Autor : |
BÁEZ, F.; CAMARGO, A.A.; GASTAL, G.D.A. |
|
Afiliación : |
FRANCISCO BÁEZ, University Center of Tacuarembó, UDELAR, Route 5, Km 386, Tacuarembó, Uruguay.; ÁLVARO A. CAMARGO, University Center of Tacuarembó, UDELAR, Route 5, Km 386, Tacuarembó, Uruguay.; GUSTAVO DESIRE ANTUNES GASTAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
|
Título : |
Ultrastructural imaging analysis of the zona pellucida surface in bovine oocytes. |
|
Fecha de publicación : |
2019 |
|
Fuente / Imprenta : |
Microscopy and Microanalysis, 1 August 2019, Volume 25, Issue 4, Pages 1032-1036. |
|
DOI : |
10.1017/S1431927619000692 |
|
Idioma : |
Inglés |
|
Notas : |
Article history: Received 21 August 2018/ Revised 23 April 2019/Accepted 23 April 2019. |
|
Contenido : |
Abstract:
The aims of the present study were to: (i) evaluate the ultrastructural differences in the zona pellucida (ZP) surface between immature and
mature bovine oocytes, and (ii) describe a new objective technique to measure the pores in the outer ZP. Intact cumulus oocyte complexes
(COCs) obtained from a local abattoir were immediately fixed (immature group) or submitted to in vitro maturation (IVM) at 38.5 °C for 24 h in a humidified atmosphere of 5% CO 2 in air (mature group). Oocytes from both groups were morphologically evaluated via Scanning Electron Microscopy (SEM) and the images were processed in the Fiji/ImageJ software using a new objective methodology through the Trainable Weka Segmentation plugin. The average number of pores in ZP was greater (p < 0.05) in the mature group than the immature group. However, the size and circularity of pores in ZP did not differ (p > 0.05) between groups. In conclusion, it has been shown that the number of pores highlighted the main ultrastructural change in the morphology of the ZP surface of bovine oocytes during the IVM pro-cess. We have described an objective method that can be used to evaluate ultrastructural modifications of the ZP surface during oocyte maturation and early embryo development. |
|
Palabras claves : |
BOVINE; IN VITRO MATURATION; PLATAFORMA SALUD ANIMAL; ULTRASTRUCTURE; ZONA PELLUCIDA. |
|
Asunto categoría : |
-- |
|
Marc : |
LEADER 02045naa a2200229 a 4500
001 1060255
005 2019-10-01
008 2019 bl uuuu u00u1 u #d
024 7 $a10.1017/S1431927619000692$2DOI
100 1 $aBÁEZ, F.
245 $aUltrastructural imaging analysis of the zona pellucida surface in bovine oocytes.$h[electronic resource]
260 $c2019
500 $aArticle history: Received 21 August 2018/ Revised 23 April 2019/Accepted 23 April 2019.
520 $aAbstract: The aims of the present study were to: (i) evaluate the ultrastructural differences in the zona pellucida (ZP) surface between immature and mature bovine oocytes, and (ii) describe a new objective technique to measure the pores in the outer ZP. Intact cumulus oocyte complexes (COCs) obtained from a local abattoir were immediately fixed (immature group) or submitted to in vitro maturation (IVM) at 38.5 °C for 24 h in a humidified atmosphere of 5% CO 2 in air (mature group). Oocytes from both groups were morphologically evaluated via Scanning Electron Microscopy (SEM) and the images were processed in the Fiji/ImageJ software using a new objective methodology through the Trainable Weka Segmentation plugin. The average number of pores in ZP was greater (p < 0.05) in the mature group than the immature group. However, the size and circularity of pores in ZP did not differ (p > 0.05) between groups. In conclusion, it has been shown that the number of pores highlighted the main ultrastructural change in the morphology of the ZP surface of bovine oocytes during the IVM pro-cess. We have described an objective method that can be used to evaluate ultrastructural modifications of the ZP surface during oocyte maturation and early embryo development.
653 $aBOVINE
653 $aIN VITRO MATURATION
653 $aPLATAFORMA SALUD ANIMAL
653 $aULTRASTRUCTURE
653 $aZONA PELLUCIDA
700 1 $aCAMARGO, A.A.
700 1 $aGASTAL, G.D.A.
773 $tMicroscopy and Microanalysis, 1 August 2019, Volume 25, Issue 4, Pages 1032-1036.
Descargar
Esconder MarcPresentar Marc Completo |
|
Registro original : |
INIA La Estanzuela (LE) |
|
|
Biblioteca
|
Identificación
|
Origen
|
Tipo / Formato
|
Clasificación
|
Cutter
|
Registro
|
Volumen
|
Estado
|
Volver
|
|
|
| Registros recuperados : 2 | |
| 1. |  | CABOT, M.I.; LADO, J.; MANZI, M.; SANJUÁN, N. Life cycle assessment of citrus tree nurseries in Uruguay: Are their environmental impacts relevant?. Environmental Impact Assessment Review. 2024, Volume 106, 107488. https://doi.org/10.1016/j.eiar.2024.107488 -- OPEN ACCESS. Article history: Received 3 August 2023, Revised 6 March 2024, Accepted 6 March 2024, Available online 15 March 2024, Version of Record 15 March 2024. -- Correspondence: Cabot, M.I.; Grup ASPA, Departament de Tecnologia d'Aliments,...| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA Las Brujas. |
|    |
| 2. | | CABOT, M.I.; LADO, J.; BAUTISTA, I.; RIBAL, J.; SANJUÁN, N. On the relevance of site specificity and temporal variability in agricultural LCA: a case study on mandarin in North Uruguay. International Journal of Life Cycle Assessment. 2023, https://doi.org/10.1007/s11367-023-02186-6 -- OPEN ACCESS. [Article in Press] Article history: Received 29 December 2022; Accepted 15 May 2023; Published 19 June 2023. -- Correspondence author: Cabot, M.I.; Grup ASPA, Departament de Tecnologia d?Aliments, Edifici 3F, Universitat Politècnica de València, Camí de...| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA Las Brujas. |
|  |
| Registros recuperados : 2 | |
|
| Expresión de búsqueda válido. Check! |
|
|
