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| Registros recuperados : 8 | |
| 3. |  | KHU, D.; REYNO, R.; BRUMMER, E.; MONTEROS, M. Screening methods for aluminum tolerance in Alfalfa. Crop Science, 2012, v. 52, p. 161 - 167. DOI: https://doi.org/10.2135/cropsci2011.05.0256 52 Article history: Published online 4 Oct. 2011. Acknowledgments:This work was funded by the Samuel Roberts Noble Foundation. We thank Christy Motes, Kazuyo Ueda, and Will Chaney
for their assistance with the whole plant assay in media and...| Biblioteca(s): INIA Tacuarembó. |
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| 4. | | REYNO, R.; DONG-MAN, K.; MONTEROS, M.J.; BOUTON, J.H.; PARROTT, W.; BRUMMER, E.C. Evaluation of two transgenes for aluminum tolerance in alfalfa. Crop Science, 2013, v. 53, no. 4, p. 1581-1588. DOI: https://doi.org/10.2135/cropsci2012.12.0676 History article: Received 3 Dec. 2012. Acknowledgments The authors would like to thank Donald Wood, Jonathan Markham, and Wesley Dean for their support with the greenhouse screening, Dr. Nathan Hancock for helping with the Southern blot...| Biblioteca(s): INIA Tacuarembó. |
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| 5. | | KHU, D.; REYNO, R.; HAN, Y.; ZHAO, P.; BOUTON, J.; BRUMMER, E.; MONTEROS, M. Identification of Aluminum Tolerance Quantitative Trait Loci in Tetraploid Alfalfa. Crop Science, 2013, v. 53, no 1, p. 148 - 163. DO: https://doi.org/10.2135/cropsci2012.03.0181 History article: Received 15 Mar. 2012. Acknowledgments: We would like to thank Kazuyo Ueda, Christy Motes, Will Chaney, Zachary Ishikawa, and Craig Schluttenhofer for their
assistance with tissue culture and Xuehui Li for developing the...| Biblioteca(s): INIA Tacuarembó. |
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| 7. | | KHU, D.; REYNO, R.; BRUMMER, E.; BOUTON, J.; HAN, Y.; MONTEROS, M. QTL mapping of aluminum tolerance in tetraploid alfalfa In: Huyghe, C., ed. Sustainable use of Genetic Diversity in Forage and Turf Breeding. Lusignan, (FR): Springer, 2010, cap. 64. 437-442| Biblioteca(s): INIA Tacuarembó. |
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| 8. | | LI, X.; ALARCÓN-ZÚÑIGA, B.; KANG, J.; TAHIR, M.H.N.; JIANG, Q.; WEI, Y.; REYNO, R.; ROBINS, J.G.; BRUMMER, E.C. Mapping fall dormancy and winter injury in tetraploid alfalfa. Crop Science, 2015, v. 55, p. 1995-2011. DOI: https://doi.org/10.2135/cropsci2014.12.0834 Article history: Received 16 Dec. 2014; Accepted 7 Apr. 2015. Acknowledgments: This research was funded in part by USDA SDA–IFAFS Grant No. 00-52100-9611 to ECB. We thank Mark Smith for fieldplot assistance.| Biblioteca(s): INIA Tacuarembó. |
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| Registros recuperados : 8 | |
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 | Acceso al texto completo restringido a Biblioteca INIA Tacuarembó. Por información adicional contacte bibliotb@tb.inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Tacuarembó. |
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Fecha actual : |
15/09/2014 |
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Actualizado : |
26/11/2020 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
REYNO, R.; DONG-MAN, K.; MONTEROS, M.J.; BOUTON, J.H.; PARROTT, W.; BRUMMER, E.C. |
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Afiliación : |
RAFAEL ALEJANDRO REYNO PODESTA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; DONG-MAN, KHU; MARÍA J. MONTEROS; JOSEPH H. BOUTON; WAYNE PARROTT; E. CHARLES BRUMMER. |
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Título : |
Evaluation of two transgenes for aluminum tolerance in alfalfa. |
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Fecha de publicación : |
2013 |
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Fuente / Imprenta : |
Crop Science, 2013, v. 53, no. 4, p. 1581-1588. DOI: https://doi.org/10.2135/cropsci2012.12.0676 |
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DOI : |
10.2135/cropsci2012.12.0676 |
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Idioma : |
Inglés |
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Notas : |
History article: Received 3 Dec. 2012. Acknowledgments The authors would like to thank Donald Wood, Jonathan Markham, and Wesley Dean for their support with the greenhouse screening, Dr. Nathan Hancock for helping with the Southern blot analysis, Yanling Wei for laboratory assistance with gene expressions, and Maria Ortega for helping with the RT-PCR. We would also like to thank Forage Genetics Intl. for providing plant materials R2336 and 60T180-14. This study was funded by the Georgia Agricultural Experiment Stations and The Samuel Roberts Noble Foundation. |
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Contenido : |
Alfalfa (Medicago sativa L.) production is dramatically reduced in acidic, Al-rich soil. Transgeni plants of several species overexpressing organic acid synthesis and/or organic acid transporter genes have shown enhanced tolerance to Al. The objective of this research was to evaluate the effect of the citrate synthase (CS) and the Daucus carota L. plasma membrane H+-transporting adenosine triphosphatase (H+ATPase) (DcPA1) transgenes on Al tolerance in alfalfa. Stem cuttings from a full-sib T2 population including four isogenic groups (plants with neither transgene, CS only, DcPA1 only,
and both transgenes) together with nontransformed check genotypes were evaluated for Al and acid soil tolerance in a greenhouse assay in limed and unlimed soil. Aluminum and acid soil tolerance was assessed by measuring the ratios of root and shoot dry weight in unlimed soil compared to limed soil. The three transgenic populations, CS, DcPA1, and CS plus DcPA1, all showed higher Al and acid oil tolerance and lower levels of Al in shoot tissue than the nontransgenic isogenic population or the nontransgenic parental genotypes. This suggests that an Al-exclusion mechanism could be driving Al and acid soil tolerance in this study. We observed no advantage of combining both transgenes in the
same genetic background. These transgenes offer an efficient method to achieve enhanced Al and acid soil tolerant alfalfa cultivars, but more information is needed on their stability across generations and genetic backgrounds and their performance under field conditions. MenosAlfalfa (Medicago sativa L.) production is dramatically reduced in acidic, Al-rich soil. Transgeni plants of several species overexpressing organic acid synthesis and/or organic acid transporter genes have shown enhanced tolerance to Al. The objective of this research was to evaluate the effect of the citrate synthase (CS) and the Daucus carota L. plasma membrane H+-transporting adenosine triphosphatase (H+ATPase) (DcPA1) transgenes on Al tolerance in alfalfa. Stem cuttings from a full-sib T2 population including four isogenic groups (plants with neither transgene, CS only, DcPA1 only,
and both transgenes) together with nontransformed check genotypes were evaluated for Al and acid soil tolerance in a greenhouse assay in limed and unlimed soil. Aluminum and acid soil tolerance was assessed by measuring the ratios of root and shoot dry weight in unlimed soil compared to limed soil. The three transgenic populations, CS, DcPA1, and CS plus DcPA1, all showed higher Al and acid oil tolerance and lower levels of Al in shoot tissue than the nontransgenic isogenic population or the nontransgenic parental genotypes. This suggests that an Al-exclusion mechanism could be driving Al and acid soil tolerance in this study. We observed no advantage of combining both transgenes in the
same genetic background. These transgenes offer an efficient method to achieve enhanced Al and acid soil tolerant alfalfa cultivars, but more information is needed on their stability across generations and ge... Presentar Todo |
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Palabras claves : |
LUCERNE. |
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Thesagro : |
ALFALFA. |
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Asunto categoría : |
A50 Investigación agraria |
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Marc : |
LEADER 02768naa a2200229 a 4500
001 1050225
005 2020-11-26
008 2013 bl uuuu u00u1 u #d
024 7 $a10.2135/cropsci2012.12.0676$2DOI
100 1 $aREYNO, R.
245 $aEvaluation of two transgenes for aluminum tolerance in alfalfa.
260 $c2013
500 $aHistory article: Received 3 Dec. 2012. Acknowledgments The authors would like to thank Donald Wood, Jonathan Markham, and Wesley Dean for their support with the greenhouse screening, Dr. Nathan Hancock for helping with the Southern blot analysis, Yanling Wei for laboratory assistance with gene expressions, and Maria Ortega for helping with the RT-PCR. We would also like to thank Forage Genetics Intl. for providing plant materials R2336 and 60T180-14. This study was funded by the Georgia Agricultural Experiment Stations and The Samuel Roberts Noble Foundation.
520 $aAlfalfa (Medicago sativa L.) production is dramatically reduced in acidic, Al-rich soil. Transgeni plants of several species overexpressing organic acid synthesis and/or organic acid transporter genes have shown enhanced tolerance to Al. The objective of this research was to evaluate the effect of the citrate synthase (CS) and the Daucus carota L. plasma membrane H+-transporting adenosine triphosphatase (H+ATPase) (DcPA1) transgenes on Al tolerance in alfalfa. Stem cuttings from a full-sib T2 population including four isogenic groups (plants with neither transgene, CS only, DcPA1 only, and both transgenes) together with nontransformed check genotypes were evaluated for Al and acid soil tolerance in a greenhouse assay in limed and unlimed soil. Aluminum and acid soil tolerance was assessed by measuring the ratios of root and shoot dry weight in unlimed soil compared to limed soil. The three transgenic populations, CS, DcPA1, and CS plus DcPA1, all showed higher Al and acid oil tolerance and lower levels of Al in shoot tissue than the nontransgenic isogenic population or the nontransgenic parental genotypes. This suggests that an Al-exclusion mechanism could be driving Al and acid soil tolerance in this study. We observed no advantage of combining both transgenes in the same genetic background. These transgenes offer an efficient method to achieve enhanced Al and acid soil tolerant alfalfa cultivars, but more information is needed on their stability across generations and genetic backgrounds and their performance under field conditions.
650 $aALFALFA
653 $aLUCERNE
700 1 $aDONG-MAN, K.
700 1 $aMONTEROS, M.J.
700 1 $aBOUTON, J.H.
700 1 $aPARROTT, W.
700 1 $aBRUMMER, E.C.
773 $tCrop Science, 2013$gv. 53, no. 4, p. 1581-1588. DOI: https://doi.org/10.2135/cropsci2012.12.0676
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