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| 3. |  | FEDERICI, M.; BAJSA, N.; LAGURARA, P.; REVALE, S.; LEONI, C.; MARCONDES, J.; DALLA RIZZA, M. Analysis of bacterial diversity in maize rhizosphere and bulk, soil using DGGE and 454-Pyrosequencing of the 165 RRNA gene. GGM 46 - COMUNICACIONES LIBRES - GGM. GENÓMICA Y GENÉTICA MOLECULAR In: JOURNAL OF BASIC & APPLIED GENETICS, 2016, Vol.27, Iss. 1 (Supp.). XVI LATIN AMERICAN CONGRESS OF GENETICS, IV CONGRESS OF THE URUGUAYAN SOCIETY OF GENETICS, XLIX ANNUAL MEETING OF THE GENETICS SOCIETY OF CHILE, XLV ARGENTINE CONGRESS OF GENETICS, 9-12 October 2016. PROCEEDINGS. Montevideo (Uruguay): SAG, 2016. p. 277| Biblioteca(s): INIA Las Brujas. |
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| 4. | | Bajsa, N.; Azziz, G.; Quagliotto, L.; Zerbino, S.; Morón, A.; Altier, N.; Arias, A. Evaluación de la biodiversidad en suelos bajo diferentes sistemas de producción ln: Reunión del Grupo Técnico Regional del Cono Sur en Mejoramiento y Utilización de los Recursos Forrajeros del Area Tropical y Subtropical, Grupo Campos, 20., 2004, Salto, UY Saldanha, S.; Bemhaja, M.; Moliterno, E.; Olmos, F.; Uriarte, G., ed. Sustentabilidad, desarrollo y conservación de los ecosistemas : memorias. Salto: UdelaR. Regional Norte, 2004. p. 347| Biblioteca(s): INIA La Estanzuela. |
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| 5. | | DE LA FUENTE, L.; BAJSA, N.; QUAGLIOTTO, L.; FABIANO, E.; PÉREZ, C.; ALTIER, N.; ARIAS, A. Interactions among Rhizobia and biocontrol agents in forage legumes. Proceedings of the 12 th International Congress on Nitrogen Fixation, Foz do Iguaçu, Paraná, Brazil, September 12?17, 1999. In: Pedrosa, F.O., Hungria, M., Yates, G., Newton, W.E. (eds). Nitrogen Fixation: From Molecules to Crop Productivity. Current Plant Science and Biotechnology in Agriculture, vol 38. Springer, Dordrecht. p. 552. https://doi.org/10.1007/0-306-47615-0_311 This work was partially supported by PEDECIBA, The European Union, and CSIC.| Biblioteca(s): INIA Las Brujas. |
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| 6. | | DE LA FUENTE, L.; QUAGLIOTTO, L.; BAJSA, N.; FABIANO, E.; ALTIER, N.; ARAS, A. Inoculation with Pseudomonas fiuorescens biocontrol strains does not affect the symbiosis between rhizobia and forage legumes. Soil Biology and Biochemistry, 2002, Volume 34, Issue 4, Pages 545-548. Article history: Received 3 november 2000 / Received in revised form 27 august 2001 / Accepted 5 september 2001| Biblioteca(s): INIA Las Brujas. |
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| 7. | | FEDERICI, M.; BAJSA, N.; LAGURARA, P.; REVALE, S.; MARCONDES, J.A.; DALLA RIZZA, M. Soil and rhizosphere bacterial diversity in maize agro-ecosystem. Sustainable Agriculture Research, 2017, v.6 (3): 35-51. Article history: Received: April 3, 2017; Accepted: May 20, 2017; Online Published: June 16, 2017.| Biblioteca(s): INIA Las Brujas. |
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| 8. | | QUAGLIOTTO, L.; AZZIZ, G.; BAJSA, N.; VAZ, P.; PÉREZ, C.; DUCAMP, F.; CADENAZZI, M.; ALTIER, N.; ARIAS, A. Three native Pseudomonas fluorescens strains tested under growth chamber and field conditions as biocontrol agents against damping-off in alfalfa. Biological Control, October 2009, Volume 51, Issue 1, Pages 42-50. Doi: https://doi.org/10.1016/j.biocontrol.2009.05.006 Article history: Received 1 September 2008 / Accepted 22 May 2009 / Available online 28 May 2009.
Corresponding author: Leticia Quagliotto - letty@iibce.edu.uy
Funding information: This work was financially supported by the LIA-INIA 028...| Biblioteca(s): INIA Las Brujas. |
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| 9. | | FEDERICI, M.; BAJSA, N.; LAUGURARA, P.; GARAYCOCHEA, S.; GIANNONE, N.; ZERBINO, M.; GARCIA, C.; LEONI, C.; TERRA, J.A.; DALLA RIZZA, M. La Metagenómica: nuevo abordaje para el estudio de la diversidad microbiana como indicador de calidad de suelos agrícolas y prospección de nuevas cepas y genes. Evaluación de la diversidad microbiana en suelos agrícolas y rizósfera de maíz transgénico. ln: Jornada técnica, VI Jornada de agrobiotecnología. INIA Las Brujas, 20 de octubre de 2012 Conocimiento intensivo para el sector productivo: situación actual y perspectivas. Canelones (UY): INIA, 2012. 28 (Serie Actividades de Difusión; 698)| Biblioteca(s): INIA Las Brujas. |
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| 10. | | SCARLATO, M.; RIEPPI, M.; ALLIAUME, F.; ILLARZE, G.; BAJSA, N.; BERTONI, P.; BIANCHI, F.J.J.A.; ECHEVERRIBORDA, G.; GALVÁN, G.; GARCÍA DE SOUZA, M.; GILSANZ, J.C.; GONZÁLEZ BARRIOS, P.; DIESTE, J.P.; TRASANTE, T.; ROSSING, W.A.H.; DOGLIOTTI, S. Towards the development of cover crop - reduced tillage systems without herbicides and synthetic fertilizers in onion cultivation: Promising but challenges remain. Soil and Tillage Research. 2024, Volume 240, Article 106061. https://doi.org/10.1016/j.still.2024.106061 --- OPEN ACCESS. Article history: Received 8 May 2023, Revised 15 February 2024, Accepted 20 February 2024, Available online 6 March 2024, Version of Record 6 March 2024. -- Correspondence: Scarlato, M.; Facultad de Agronomía, Universidad de la...| Biblioteca(s): INIA Las Brujas. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
09/11/2017 |
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Actualizado : |
12/12/2018 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
FEDERICI, M.; BAJSA, N.; LAGURARA, P.; REVALE, S.; MARCONDES, J.A.; DALLA RIZZA, M. |
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Afiliación : |
MARIA TERESA FEDERICI RODRIGUEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; NATALIA BAJSA VALVERDE, MEC/ IIBCE (Instituto de Investigaciones de Ciencias Biológicas "Clemente Estable"); PAULA LAGURARA, MEC/ IIBCE (Instituto de Investigaciones de Ciencias Biológicas "Clemente Estable"); SANTIAGO REVALE, INDEAR (Instituto de Agrobiotecnología de Rosario); JACKSON A. MARCONDES, Universidad Estadual Paulista (UNESP)/ Campus de Jaboticabal; MARCO DALLA RIZZA VILARO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Soil and rhizosphere bacterial diversity in maize agro-ecosystem. |
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Fecha de publicación : |
2017 |
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Fuente / Imprenta : |
Sustainable Agriculture Research, 2017, v.6 (3): 35-51. |
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ISSN : |
1927-050X (Print) // 1927-0518 (Online) |
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DOI : |
10.5539/sar.v6n3p |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: April 3, 2017; Accepted: May 20, 2017; Online Published: June 16, 2017. |
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Contenido : |
ABSTRACT.
Management practices used in maize production have an impact on soil agro- ecosystems where different microbial communities coexist. Soil inhabiting bacteria are numerous and diverse, but we know very little about their ecological distribution. Here we analyzed the bacterial community diversity in the rhizosphere of two transgenic maize cultivars, in agricultural soil before sowing and in non-cultivated soil in an experimental site in the south region of Uruguay. We followed two culture-independent methods: DGGE (denaturing gradient gel electrophoresis) and 454-pyrosequencing of 16S rRNA gene amplicon. Through pyrosequencing, the three environments analyzed presented differences in terms of bacterial composition. However, no differences were found in the relative abundance of the ten most represented phyla in the rhizosphere of the two cultivars at different phenological stages. We found significant differences of Bacteroidetes, Gemmatimonadetes, Planctomycetes, Proteobacteria and Verrucomicrobia phyla when comparing agricultural and non-cultivated soils, as well as a significant enrichment of members of the phylum Gemmatimonadetes in all rhizosphere samples compared to soil. Through DGGE analysis we evidenced that maize rhizosphere bacterial communities changed at different phenological stages in both cultivars. We also provided baseline information about bacterial specific taxa within maize agro- ecosystem for further evaluation of possible rhizosphere bacterial community shifts of genetically modified maize cultivars under different management practices.
Copyright © Canadian Center of Science and Education MenosABSTRACT.
Management practices used in maize production have an impact on soil agro- ecosystems where different microbial communities coexist. Soil inhabiting bacteria are numerous and diverse, but we know very little about their ecological distribution. Here we analyzed the bacterial community diversity in the rhizosphere of two transgenic maize cultivars, in agricultural soil before sowing and in non-cultivated soil in an experimental site in the south region of Uruguay. We followed two culture-independent methods: DGGE (denaturing gradient gel electrophoresis) and 454-pyrosequencing of 16S rRNA gene amplicon. Through pyrosequencing, the three environments analyzed presented differences in terms of bacterial composition. However, no differences were found in the relative abundance of the ten most represented phyla in the rhizosphere of the two cultivars at different phenological stages. We found significant differences of Bacteroidetes, Gemmatimonadetes, Planctomycetes, Proteobacteria and Verrucomicrobia phyla when comparing agricultural and non-cultivated soils, as well as a significant enrichment of members of the phylum Gemmatimonadetes in all rhizosphere samples compared to soil. Through DGGE analysis we evidenced that maize rhizosphere bacterial communities changed at different phenological stages in both cultivars. We also provided baseline information about bacterial specific taxa within maize agro- ecosystem for further evaluation of possible rhizosphere bacterial ... Presentar Todo |
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Palabras claves : |
DGGE; METAGENOMICS; PYROSEQUENCING; RHIZOSPHERE; SOIL BACTERIA; TRANSGENIC MAIZE. |
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Asunto categoría : |
-- |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/7925/1/67345-252782-1-PB.pdf
http://www.ccsenet.org/journal/index.php/sar/article/view/67345/37668
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Marc : |
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022 $a1927-050X (Print) // 1927-0518 (Online)
024 7 $a10.5539/sar.v6n3p$2DOI
100 1 $aFEDERICI, M.
245 $aSoil and rhizosphere bacterial diversity in maize agro-ecosystem.$h[electronic resource]
260 $c2017
500 $aArticle history: Received: April 3, 2017; Accepted: May 20, 2017; Online Published: June 16, 2017.
520 $aABSTRACT. Management practices used in maize production have an impact on soil agro- ecosystems where different microbial communities coexist. Soil inhabiting bacteria are numerous and diverse, but we know very little about their ecological distribution. Here we analyzed the bacterial community diversity in the rhizosphere of two transgenic maize cultivars, in agricultural soil before sowing and in non-cultivated soil in an experimental site in the south region of Uruguay. We followed two culture-independent methods: DGGE (denaturing gradient gel electrophoresis) and 454-pyrosequencing of 16S rRNA gene amplicon. Through pyrosequencing, the three environments analyzed presented differences in terms of bacterial composition. However, no differences were found in the relative abundance of the ten most represented phyla in the rhizosphere of the two cultivars at different phenological stages. We found significant differences of Bacteroidetes, Gemmatimonadetes, Planctomycetes, Proteobacteria and Verrucomicrobia phyla when comparing agricultural and non-cultivated soils, as well as a significant enrichment of members of the phylum Gemmatimonadetes in all rhizosphere samples compared to soil. Through DGGE analysis we evidenced that maize rhizosphere bacterial communities changed at different phenological stages in both cultivars. We also provided baseline information about bacterial specific taxa within maize agro- ecosystem for further evaluation of possible rhizosphere bacterial community shifts of genetically modified maize cultivars under different management practices. Copyright © Canadian Center of Science and Education
653 $aDGGE
653 $aMETAGENOMICS
653 $aPYROSEQUENCING
653 $aRHIZOSPHERE
653 $aSOIL BACTERIA
653 $aTRANSGENIC MAIZE
700 1 $aBAJSA, N.
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700 1 $aREVALE, S.
700 1 $aMARCONDES, J.A.
700 1 $aDALLA RIZZA, M.
773 $tSustainable Agriculture Research, 2017$gv.6 (3): 35-51.
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