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| Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
07/02/2023 |
Actualizado : |
24/04/2023 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
DA CUNHA, L. L.; BREMM, C.; SAVIAN, J.V.; ZUBIETA, Á. S.; ROSSETTO, J.; CARVALHO, P. C. DE F. |
Afiliación : |
LAIS LEAL DA CUNHA, LAIS LEAL, Grazing Ecology Research Group, Federal University of Rio Grande Do Sul, RS, Porto Alegre, Brazil; CAROLINA BREMM, State Foundation of Agricultural Research, Rua Gonçalves Dias, 570, Bairro Menino Deus, RS, Porto Alegre, Brazil; JEAN VICTOR SAVIAN, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ÁNGEL SANCHEZ ZUBIETA, Grazing Ecology Research Group, Federal University of Rio Grande Do Sul, RS, Porto Alegre, Brazil; JUSIANE ROSSETTO, Grazing Ecology Research Group, Federal University of Rio Grande Do Sul, RS, Porto Alegre, Brazil; PAULO CÉSAR DE FACCIO CARVALHO, Grazing Ecology Research Group, Federal University of Rio Grande Do Sul, RS, Porto Alegre, Brazil. |
Título : |
Relevance of sward structure and forage nutrient contents in explaining methane emissions from grazing beef cattle and sheep. |
Fecha de publicación : |
2023 |
Fuente / Imprenta : |
Science of the Total Environment, 2023. Volume 869, Article number 161695. doi: https://doi.org/10.1016/j.scitotenv.2023.161695 |
ISSN : |
0048-9697 |
DOI : |
10.1016/j.scitotenv.2023.161695 |
Idioma : |
Inglés |
Notas : |
Article history: Received 18 March 2022; Received in revised form 30 December 2022; Accepted 15 January 2023; Available online 21 January 2023; To be published 15 April 2023. -- Corresponding author: da Cunha, L.L.; Grazing Ecology Research Group, Department of Forage Plants and Agrometeorology, Faculty of Agronomy, Federal University of Rio Grande Do Sul, Bento Gonçalves Ave., 7712, RS, Porto Alegre, Brazil; email:laiscvet@gmail.com -- Editor: Kuishuang Feng -- |
Contenido : |
ABSTRACT.- Forage nutrient contents are an important factor explaining the dry matter intake (DMI), average daily gain (ADG), and methane emissions (CH4) of ruminants fed indoors. However, for grazing animals, the forage nutrient contents might be limited in explaining such response variables. We aimed to verify the explanatory power of forage nutrient contents and sward structure on daily intake, performance, and CH4 emissions by sheep and beef cattle grazing different grassland types in southern Brazil. We analyzed data from five grazing trials using sheep and beef cattle grazing on Italian ryegrass (Lolium multiflorum), mixed Italian ryegrass and black oat (Lolium multiflorum + Avena strigosa), pearl millet (Pennisetum americanum), and multispecies native grassland. We used mixed models, including the forage nutrient contents [crude protein (CP), neutral detergent fiber (NDF), and acid detergent fiber (ADF)], sward structure (sward height and herbage mass) and their interactions, as fixed effects and trial, season, methodologies, animal species, grassland type, and paddock, as random effects. The model for DMI (kg DM/LW0.75) had an adjusted coefficient of determination (R2adj) of 71.6 %, where 11.3, 23.1, and 37.2 % of the R2adj were explained by the forage nutrient contents, sward structure, and their interaction, respectively. The ADG (kg/LW0.75) model presented an R2adj of 74.2 %, with 12.5 % explained by forage nutrient contents, 29.3 % by sward structure, and 32.4 % by their interaction. The daily CH4 emission (g/LW0.75) model had a lower adjusted coefficient of determination (R2adj = 47.6 %), with 16.8 % explained by forage nutrient contents and 30.8 % explained by sward structure, but no effect of the interaction. Our results show that in grazing ecosystems, the forage nutrient contents explain a small fraction, and the greater explanatory power for DMI, ADG, and CH4 emissions models is related to sward structure descriptors, such as sward height and herbage mass. Moreover, the interaction between these variables explains most of the variation. In conclusion, forage nutrient contents and sward structure have different influences on DMI, ADG, and CH4 emissions by grazing ruminants. Because of its relevance to daily CH4 emissions, offering an optimal sward structure to grazing animals is a major climate-smart strategy to improve animal production and mitigate CH4 emissions in pastoral ecosystems. © 2023 Elsevier B.V. MenosABSTRACT.- Forage nutrient contents are an important factor explaining the dry matter intake (DMI), average daily gain (ADG), and methane emissions (CH4) of ruminants fed indoors. However, for grazing animals, the forage nutrient contents might be limited in explaining such response variables. We aimed to verify the explanatory power of forage nutrient contents and sward structure on daily intake, performance, and CH4 emissions by sheep and beef cattle grazing different grassland types in southern Brazil. We analyzed data from five grazing trials using sheep and beef cattle grazing on Italian ryegrass (Lolium multiflorum), mixed Italian ryegrass and black oat (Lolium multiflorum + Avena strigosa), pearl millet (Pennisetum americanum), and multispecies native grassland. We used mixed models, including the forage nutrient contents [crude protein (CP), neutral detergent fiber (NDF), and acid detergent fiber (ADF)], sward structure (sward height and herbage mass) and their interactions, as fixed effects and trial, season, methodologies, animal species, grassland type, and paddock, as random effects. The model for DMI (kg DM/LW0.75) had an adjusted coefficient of determination (R2adj) of 71.6 %, where 11.3, 23.1, and 37.2 % of the R2adj were explained by the forage nutrient contents, sward structure, and their interaction, respectively. The ADG (kg/LW0.75) model presented an R2adj of 74.2 %, with 12.5 % explained by forage nutrient contents, 29.3 % by sward structure, and 32.4 % ... Presentar Todo |
Palabras claves : |
Forage chemical composition; Grasslands; Livestock; Pastoral ecosystems; Sward height. |
Asunto categoría : |
A50 Investigación agraria |
Marc : |
LEADER 03850naa a2200277 a 4500 001 1063954 005 2023-04-24 008 2023 bl uuuu u00u1 u #d 022 $a0048-9697 024 7 $a10.1016/j.scitotenv.2023.161695$2DOI 100 1 $aDA CUNHA, L. L. 245 $aRelevance of sward structure and forage nutrient contents in explaining methane emissions from grazing beef cattle and sheep.$h[electronic resource] 260 $c2023 500 $aArticle history: Received 18 March 2022; Received in revised form 30 December 2022; Accepted 15 January 2023; Available online 21 January 2023; To be published 15 April 2023. -- Corresponding author: da Cunha, L.L.; Grazing Ecology Research Group, Department of Forage Plants and Agrometeorology, Faculty of Agronomy, Federal University of Rio Grande Do Sul, Bento Gonçalves Ave., 7712, RS, Porto Alegre, Brazil; email:laiscvet@gmail.com -- Editor: Kuishuang Feng -- 520 $aABSTRACT.- Forage nutrient contents are an important factor explaining the dry matter intake (DMI), average daily gain (ADG), and methane emissions (CH4) of ruminants fed indoors. However, for grazing animals, the forage nutrient contents might be limited in explaining such response variables. We aimed to verify the explanatory power of forage nutrient contents and sward structure on daily intake, performance, and CH4 emissions by sheep and beef cattle grazing different grassland types in southern Brazil. We analyzed data from five grazing trials using sheep and beef cattle grazing on Italian ryegrass (Lolium multiflorum), mixed Italian ryegrass and black oat (Lolium multiflorum + Avena strigosa), pearl millet (Pennisetum americanum), and multispecies native grassland. We used mixed models, including the forage nutrient contents [crude protein (CP), neutral detergent fiber (NDF), and acid detergent fiber (ADF)], sward structure (sward height and herbage mass) and their interactions, as fixed effects and trial, season, methodologies, animal species, grassland type, and paddock, as random effects. The model for DMI (kg DM/LW0.75) had an adjusted coefficient of determination (R2adj) of 71.6 %, where 11.3, 23.1, and 37.2 % of the R2adj were explained by the forage nutrient contents, sward structure, and their interaction, respectively. The ADG (kg/LW0.75) model presented an R2adj of 74.2 %, with 12.5 % explained by forage nutrient contents, 29.3 % by sward structure, and 32.4 % by their interaction. The daily CH4 emission (g/LW0.75) model had a lower adjusted coefficient of determination (R2adj = 47.6 %), with 16.8 % explained by forage nutrient contents and 30.8 % explained by sward structure, but no effect of the interaction. Our results show that in grazing ecosystems, the forage nutrient contents explain a small fraction, and the greater explanatory power for DMI, ADG, and CH4 emissions models is related to sward structure descriptors, such as sward height and herbage mass. Moreover, the interaction between these variables explains most of the variation. In conclusion, forage nutrient contents and sward structure have different influences on DMI, ADG, and CH4 emissions by grazing ruminants. Because of its relevance to daily CH4 emissions, offering an optimal sward structure to grazing animals is a major climate-smart strategy to improve animal production and mitigate CH4 emissions in pastoral ecosystems. © 2023 Elsevier B.V. 653 $aForage chemical composition 653 $aGrasslands 653 $aLivestock 653 $aPastoral ecosystems 653 $aSward height 700 1 $aBREMM, C. 700 1 $aSAVIAN, J.V. 700 1 $aZUBIETA, Á. S. 700 1 $aROSSETTO, J. 700 1 $aCARVALHO, P. C. DE F. 773 $tScience of the Total Environment, 2023. Volume 869, Article number 161695. doi: https://doi.org/10.1016/j.scitotenv.2023.161695
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| Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha actual : |
29/07/2020 |
Actualizado : |
03/12/2020 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Circulación / Nivel : |
-- - -- |
Autor : |
NARANCIO, R.; DING, Y.-L.; LIN, Y.-H.; SAHAB, S.; PANTER, S.; HAYES, M.; JOHN, U.; ANDERSON, H.; MASON, J.; SPANGENBERG, G. |
Afiliación : |
RAFAEL NARANCIO FERES, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; Agriculture Victoria Research, AgriBio, Centre for AgriBioscience, Bundoora, Australia; School of Applied Systems Biology, La Trobe University, Bundoora, Australia; YONG?LIN DING, Agriculture Victoria Research, AgriBio, Centre for AgriBioscience, Bundoora, Australia; YI?HAN LIN, Agriculture Victoria Research, AgriBio, Centre for AgriBioscience, Bundoora, Australia; SAREENA SAHAB, Agriculture Victoria Research, AgriBio, Centre for AgriBioscience, Bundoora, Australia; STEPHEN PANTER, Agriculture Victoria Research, AgriBio, Centre for AgriBioscience, Bundoora, Australia; MATTHEW HAYES, Triffid BioScience, PO Box 1986, Carlton South, Australia; ULRIK JOHN, Agriculture Victoria Research, AgriBio, Centre for AgriBioscience, Bundoora, Australia; HEATHER ANDERSON, Agriculture Victoria Research, AgriBio, Centre for AgriBioscience, Bundoora, Australia; JOHN MASON, Agriculture Victoria Research, AgriBio, Centre for AgriBioscience, Bundoora, Australia; School of Applied Systems Biology, La Trobe University, Bundoora, Australia; GERMAN SPANGENBERG, Agriculture Victoria Research, AgriBio, Centre for AgriBioscience, Bundoora, Australia; School of Applied Systems Biology, La Trobe University, Bundoora, Australia. |
Título : |
Application of linked and unlinked co transformation to generate triple stack, marker free, transgenic white clover (Trifolium repens L.). |
Fecha de publicación : |
2020 |
Fuente / Imprenta : |
Plant Cell, Tissue and Organ Culture, 2020. DOI: https://doi.org/10.1007/s11240-020-01891-6 |
ISSN : |
0167-6857 |
DOI : |
10.1007/s11240-020-01891-6 |
Idioma : |
Inglés |
Notas : |
Article history: Received: 19 May 2020 / Accepted: 15 July 2020. |
Contenido : |
ABSTRACT.
Both linked and un-linked co-transformation can be used to overcome limitations of methods, such as re-transformation or sexual crossing of transgenic plants, to enable transfer of multiple genes to a single plant. Un-linked co-transformation can also facilitate the production of selectable marker-free transgenic plants. In this study, transgenic white clover plants were generated by Agrobacterium-mediated linked co-transformation using a single T-DNA of 9803 bp expressing: an isopentenyl transferase (IPT) gene for delayed leaf senescence under the control of an organ specific MYB32 promoter from Arabidopsis, a white clover nodule enhanced malate dehydrogenase (neMDH) gene for aluminium tolerance controlled by the endogenous Phosphate Transporter 1 (PT1) promoter, and the coat protein gene from Alfalfa Mosaic Virus (CP-AMV) controlled by the 35S promoter from Cauliflower Mosaic Virus. The selectable marker gene encoding hygromycin phosphotransferase (hph) was borne on a separate T-DNA. Forty independent transgenic events carrying the triple stack were generated, with estimated co-transformation efficiencies of 0.22 to 0.23%. Forty three percent of the events generated had a single insertion, while two events were selectable marker-free. Transcript abundance studies of the three transgenes of interest demonstrated the transcriptional competence of the inserted T-DNA. This study illustrates the feasibility of transferring multiple genes in a large single T-DNA into white clover by Agrobacterium-mediated co-transformation. Furthermore, observations of consistently delayed leaf senescence, statistically significant increases in TrneMDH transcript, and presence of CP-AMV transcript, support further analysis of these events for delayed leaf senescence under drought conditions, aluminium tolerance, and resistance to AMV.
© 2020, Springer Nature B.V. MenosABSTRACT.
Both linked and un-linked co-transformation can be used to overcome limitations of methods, such as re-transformation or sexual crossing of transgenic plants, to enable transfer of multiple genes to a single plant. Un-linked co-transformation can also facilitate the production of selectable marker-free transgenic plants. In this study, transgenic white clover plants were generated by Agrobacterium-mediated linked co-transformation using a single T-DNA of 9803 bp expressing: an isopentenyl transferase (IPT) gene for delayed leaf senescence under the control of an organ specific MYB32 promoter from Arabidopsis, a white clover nodule enhanced malate dehydrogenase (neMDH) gene for aluminium tolerance controlled by the endogenous Phosphate Transporter 1 (PT1) promoter, and the coat protein gene from Alfalfa Mosaic Virus (CP-AMV) controlled by the 35S promoter from Cauliflower Mosaic Virus. The selectable marker gene encoding hygromycin phosphotransferase (hph) was borne on a separate T-DNA. Forty independent transgenic events carrying the triple stack were generated, with estimated co-transformation efficiencies of 0.22 to 0.23%. Forty three percent of the events generated had a single insertion, while two events were selectable marker-free. Transcript abundance studies of the three transgenes of interest demonstrated the transcriptional competence of the inserted T-DNA. This study illustrates the feasibility of transferring multiple genes in a large single T-DNA into w... Presentar Todo |
Palabras claves : |
Agrobacterium-mediated transformation; Delayed leaf senescence; Multigene; T-DNA. |
Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
Marc : |
LEADER 02904naa a2200313 a 4500 001 1061249 005 2020-12-03 008 2020 bl uuuu u00u1 u #d 022 $a0167-6857 024 7 $a10.1007/s11240-020-01891-6$2DOI 100 1 $aNARANCIO, R. 245 $aApplication of linked and unlinked co transformation to generate triple stack, marker free, transgenic white clover (Trifolium repens L.).$h[electronic resource] 260 $c2020 500 $aArticle history: Received: 19 May 2020 / Accepted: 15 July 2020. 520 $aABSTRACT. Both linked and un-linked co-transformation can be used to overcome limitations of methods, such as re-transformation or sexual crossing of transgenic plants, to enable transfer of multiple genes to a single plant. Un-linked co-transformation can also facilitate the production of selectable marker-free transgenic plants. In this study, transgenic white clover plants were generated by Agrobacterium-mediated linked co-transformation using a single T-DNA of 9803 bp expressing: an isopentenyl transferase (IPT) gene for delayed leaf senescence under the control of an organ specific MYB32 promoter from Arabidopsis, a white clover nodule enhanced malate dehydrogenase (neMDH) gene for aluminium tolerance controlled by the endogenous Phosphate Transporter 1 (PT1) promoter, and the coat protein gene from Alfalfa Mosaic Virus (CP-AMV) controlled by the 35S promoter from Cauliflower Mosaic Virus. The selectable marker gene encoding hygromycin phosphotransferase (hph) was borne on a separate T-DNA. Forty independent transgenic events carrying the triple stack were generated, with estimated co-transformation efficiencies of 0.22 to 0.23%. Forty three percent of the events generated had a single insertion, while two events were selectable marker-free. Transcript abundance studies of the three transgenes of interest demonstrated the transcriptional competence of the inserted T-DNA. This study illustrates the feasibility of transferring multiple genes in a large single T-DNA into white clover by Agrobacterium-mediated co-transformation. Furthermore, observations of consistently delayed leaf senescence, statistically significant increases in TrneMDH transcript, and presence of CP-AMV transcript, support further analysis of these events for delayed leaf senescence under drought conditions, aluminium tolerance, and resistance to AMV. © 2020, Springer Nature B.V. 653 $aAgrobacterium-mediated transformation 653 $aDelayed leaf senescence 653 $aMultigene 653 $aT-DNA 700 1 $aDING, Y.-L. 700 1 $aLIN, Y.-H. 700 1 $aSAHAB, S. 700 1 $aPANTER, S. 700 1 $aHAYES, M. 700 1 $aJOHN, U. 700 1 $aANDERSON, H. 700 1 $aMASON, J. 700 1 $aSPANGENBERG, G. 773 $tPlant Cell, Tissue and Organ Culture, 2020. DOI: https://doi.org/10.1007/s11240-020-01891-6
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