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Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
22/01/2020 |
Actualizado : |
24/02/2022 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
RODRIGO, M.J.; LADO, J.; ALÓS, E.; ALQUÉZAR, B.; DERY, O.; HIRSCHBERG, J.; ZACARÍAS, L. |
Afiliación : |
MARÍA JESÚS RODRIGO, Instituto de Agroquímica y Tecnología de Alimentos (IATA), Consejo Superior de Investigaciones Científicas (CSIC), Spain; JOANNA LADO LINDNER, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; Instituto de Agroquímica y Tecnología de Alimentos (IATA), Consejo Superior de Investigaciones Científicas (CSIC), Spain; ENRIQUETA ALÓS, Instituto de Agroquímica y Tecnología de Alimentos (IATA), Consejo Superior de Investigaciones Científicas (CSIC), Spain; BERTA ALQUÉZAR, Instituto de Agroquímica y Tecnología de Alimentos (IATA), Consejo Superior de Investigaciones Científicas (CSIC), Spain; Instituto de Biología Molecular y Celular de Plantas (IBMCP), UPV-CSIC, Spain; ORLY DERY, Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, Jerusalem, Israel; JOSEPH HIRSCHBERG, Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, Jerusalem, Israel; LORENZO ZACARÍAS, Instituto de Agroquímica y Tecnología de Alimentos (IATA), Consejo Superior de Investigaciones Científicas (CSIC), Spain. |
Título : |
A mutant allele of ζ-carotene isomerase (Z-ISO) is associated with the yellow pigmentation of the "pinalate" sweet orange mutant and reveals new insights into its role in fruit carotenogenesis. |
Fecha de publicación : |
2019 |
Fuente / Imprenta : |
BMC Plant Biology, 4 November 2019, Volume 19, Issue 1, Article number 465. OPEN ACCESS. Doi: DOI: 10.1186/s12870-019-2078-2 |
ISSN : |
1471-2229 |
DOI : |
10.1186/s12870-019-2078-2 |
Idioma : |
Inglés |
Notas : |
Article history: Received: 17 June 2019 / Accepted: 16 October 2019 / Published online: 4 November 2019.
Funding text: Financial support of the research grants AGL2012?34576 and AGL2015? 70218 (Ministerio Economía y Competitividad, Spain), and RTI2018-095131-BI00 (Ministerio Ciencia, Innovacion y Universidades, Spain). Work in the group of JH was supported by Israel Science Foundation Grant 850/13. MJR, LZ, JH and OD are members of Eurocaroten (COST_Action CA15136). MJR and LZ belong to CaRed (Spanish Carotenoid Network BIO2017?90877-REDT, Minis-terio de Ciencia, Innovación y Universidades, Spain). Authors acknowledge support of the publication fee by the CSIC Open Access Publication Support Initiative through its Unit of Information Resources for Research (URICI).
Supplementary information accompanies this paper. |
Contenido : |
Abstract.
Background: Fruit coloration is one of the main quality parameters of Citrus fruit primarily determined by genetic factors. The fruit of ordinary sweet orange (Citrus sinensis) displays a pleasant orange tint due to accumulation of carotenoids, representing β,β-xanthophylls more than 80% of the total content. 'Pinalate' is a spontaneous bud mutant, or somatic mutation, derived from sweet orange 'Navelate', characterized by yellow fruits due to elevated proportions of upstream carotenes and reduced β,β-xanthophylls, which suggests a biosynthetic blockage at early steps of the carotenoid pathway. Results: To identify the molecular basis of 'Pinalate' yellow fruit, a complete characterization of carotenoids profile together with transcriptional changes in carotenoid biosynthetic genes were performed in mutant and parental fruits during development and ripening. 'Pinalate' fruit showed a distinctive carotenoid profile at all ripening stages, accumulating phytoene, phytofluene and unusual proportions of 9,15,9′-tri-cis- and 9,9′-di-cis-ζ-carotene, while content of downstream carotenoids was significantly decreased. Transcript levels for most of the carotenoid biosynthetic genes showed no alterations in 'Pinalate'; however, the steady-state level mRNA of ζ-carotene isomerase (Z-ISO), which catalyses the conversion of 9,15,9′-tri-cis- to 9,9′-di-cis-ζ-carotene, was significantly reduced both in 'Pinalate' fruit and leaf tissues. Isolation of the 'Pinalate' Z-ISO genomic sequence identified a new allele with a single nucleotide insertion at the second exon, which generates an alternative splicing site that alters Z-ISO transcripts encoding non-functional enzyme. Moreover, functional assays of citrus Z-ISO in E.coli showed that light is able to enhance a non-enzymatic isomerization of tri-cis to di-cis-ζ-carotene, which is in agreement with the partial rescue of mutant phenotype when 'Pinalate' fruits are highly exposed to light during ripening. Conclusion: A single nucleotide insertion has been identified in 'Pinalate' Z-ISO gene that results in truncated proteins. This causes a bottleneck in the carotenoid pathway with an unbalanced content of carotenes upstream to β,β-xanthophylls in fruit tissues. In chloroplastic tissues, the effects of Z-ISO alteration are mainly manifested as a reduction in total carotenoid content. Taken together, our results indicate that the spontaneous single nucleotide insertion in Z-ISO is the molecular basis of the yellow pigmentation in 'Pinalate' sweet orange and points this isomerase as an essential activity for carotenogenesis in citrus fruits.
© 2019 The Author(s). MenosAbstract.
Background: Fruit coloration is one of the main quality parameters of Citrus fruit primarily determined by genetic factors. The fruit of ordinary sweet orange (Citrus sinensis) displays a pleasant orange tint due to accumulation of carotenoids, representing β,β-xanthophylls more than 80% of the total content. 'Pinalate' is a spontaneous bud mutant, or somatic mutation, derived from sweet orange 'Navelate', characterized by yellow fruits due to elevated proportions of upstream carotenes and reduced β,β-xanthophylls, which suggests a biosynthetic blockage at early steps of the carotenoid pathway. Results: To identify the molecular basis of 'Pinalate' yellow fruit, a complete characterization of carotenoids profile together with transcriptional changes in carotenoid biosynthetic genes were performed in mutant and parental fruits during development and ripening. 'Pinalate' fruit showed a distinctive carotenoid profile at all ripening stages, accumulating phytoene, phytofluene and unusual proportions of 9,15,9′-tri-cis- and 9,9′-di-cis-ζ-carotene, while content of downstream carotenoids was significantly decreased. Transcript levels for most of the carotenoid biosynthetic genes showed no alterations in 'Pinalate'; however, the steady-state level mRNA of ζ-carotene isomerase (Z-ISO), which catalyses the conversion of 9,15,9′-tri-cis- to 9,9′-di-cis-ζ-carotene, was significantly reduced both in 'Pinalate' fruit an... Presentar Todo |
Palabras claves : |
Carotenoid; GENE EXPRESSION; MUTANT; Pigmentation; PLATAFORMA AGROALIMENTOS; RIPENING; Zeta-carotene isomerase. |
Thesagro : |
CAROTENOIDES; CITRUS. |
Asunto categoría : |
F01 Cultivo |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/14051/1/BMC-Plant-Pathology-2019-s12870-019-2078-2.pdf
https://bmcplantbiol.biomedcentral.com/track/pdf/10.1186/s12870-019-2078-2
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Marc : |
LEADER 04646naa a2200337 a 4500 001 1060661 005 2022-02-24 008 2019 bl uuuu u00u1 u #d 022 $a1471-2229 024 7 $a10.1186/s12870-019-2078-2$2DOI 100 1 $aRODRIGO, M.J. 245 $aA mutant allele of ζ-carotene isomerase (Z-ISO) is associated with the yellow pigmentation of the "pinalate" sweet orange mutant and reveals new insights into its role in fruit carotenogenesis.$h[electronic resource] 260 $c2019 500 $aArticle history: Received: 17 June 2019 / Accepted: 16 October 2019 / Published online: 4 November 2019. Funding text: Financial support of the research grants AGL2012?34576 and AGL2015? 70218 (Ministerio Economía y Competitividad, Spain), and RTI2018-095131-BI00 (Ministerio Ciencia, Innovacion y Universidades, Spain). Work in the group of JH was supported by Israel Science Foundation Grant 850/13. MJR, LZ, JH and OD are members of Eurocaroten (COST_Action CA15136). MJR and LZ belong to CaRed (Spanish Carotenoid Network BIO2017?90877-REDT, Minis-terio de Ciencia, Innovación y Universidades, Spain). Authors acknowledge support of the publication fee by the CSIC Open Access Publication Support Initiative through its Unit of Information Resources for Research (URICI). Supplementary information accompanies this paper. 520 $aAbstract. Background: Fruit coloration is one of the main quality parameters of Citrus fruit primarily determined by genetic factors. The fruit of ordinary sweet orange (Citrus sinensis) displays a pleasant orange tint due to accumulation of carotenoids, representing β,β-xanthophylls more than 80% of the total content. 'Pinalate' is a spontaneous bud mutant, or somatic mutation, derived from sweet orange 'Navelate', characterized by yellow fruits due to elevated proportions of upstream carotenes and reduced β,β-xanthophylls, which suggests a biosynthetic blockage at early steps of the carotenoid pathway. Results: To identify the molecular basis of 'Pinalate' yellow fruit, a complete characterization of carotenoids profile together with transcriptional changes in carotenoid biosynthetic genes were performed in mutant and parental fruits during development and ripening. 'Pinalate' fruit showed a distinctive carotenoid profile at all ripening stages, accumulating phytoene, phytofluene and unusual proportions of 9,15,9′-tri-cis- and 9,9′-di-cis-ζ-carotene, while content of downstream carotenoids was significantly decreased. Transcript levels for most of the carotenoid biosynthetic genes showed no alterations in 'Pinalate'; however, the steady-state level mRNA of ζ-carotene isomerase (Z-ISO), which catalyses the conversion of 9,15,9′-tri-cis- to 9,9′-di-cis-ζ-carotene, was significantly reduced both in 'Pinalate' fruit and leaf tissues. Isolation of the 'Pinalate' Z-ISO genomic sequence identified a new allele with a single nucleotide insertion at the second exon, which generates an alternative splicing site that alters Z-ISO transcripts encoding non-functional enzyme. Moreover, functional assays of citrus Z-ISO in E.coli showed that light is able to enhance a non-enzymatic isomerization of tri-cis to di-cis-ζ-carotene, which is in agreement with the partial rescue of mutant phenotype when 'Pinalate' fruits are highly exposed to light during ripening. Conclusion: A single nucleotide insertion has been identified in 'Pinalate' Z-ISO gene that results in truncated proteins. This causes a bottleneck in the carotenoid pathway with an unbalanced content of carotenes upstream to β,β-xanthophylls in fruit tissues. In chloroplastic tissues, the effects of Z-ISO alteration are mainly manifested as a reduction in total carotenoid content. Taken together, our results indicate that the spontaneous single nucleotide insertion in Z-ISO is the molecular basis of the yellow pigmentation in 'Pinalate' sweet orange and points this isomerase as an essential activity for carotenogenesis in citrus fruits. © 2019 The Author(s). 650 $aCAROTENOIDES 650 $aCITRUS 653 $aCarotenoid 653 $aGENE EXPRESSION 653 $aMUTANT 653 $aPigmentation 653 $aPLATAFORMA AGROALIMENTOS 653 $aRIPENING 653 $aZeta-carotene isomerase 700 1 $aLADO, J. 700 1 $aALÓS, E. 700 1 $aALQUÉZAR, B. 700 1 $aDERY, O. 700 1 $aHIRSCHBERG, J. 700 1 $aZACARÍAS, L. 773 $tBMC Plant Biology, 4 November 2019, Volume 19, Issue 1, Article number 465. OPEN ACCESS. Doi: DOI: 10.1186/s12870-019-2078-2
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INIA Las Brujas (LB) |
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Biblioteca (s) : |
INIA Tacuarembó. |
Fecha actual : |
22/12/2020 |
Actualizado : |
22/12/2020 |
Tipo de producción científica : |
Capítulo en Libro Técnico-Científico |
Autor : |
GALUSSI, A. |
Afiliación : |
ALBERTO GALUSSI, Facultad de Ciencias Agropecuarias, Universidad Nacional de Entre Ríos, República Argentina. |
Título : |
Aspectos botánicos y agronómiccos de Mnesithea Selloana (Hack) de Koning & Sosef (Cola de lagarto, Teyú Ruguay), Paraná, Entre Ríos, Repúbica Argentina. |
Fecha de publicación : |
2020 |
Fuente / Imprenta : |
In: Olmos, F. Ecofisiología de especies forrajeras del campo natural. Montevideo (UY): INIA, 2020. |
Páginas : |
p. 8-15 |
Serie : |
(INIA Serie Técnica; 256). |
ISBN : |
e-ISBN: 978-9974-38-449-1 |
ISSN : |
1688-9266 |
DOI : |
10.35676/INIA/ST.256 |
Idioma : |
Español |
Contenido : |
La información que se brinda en este apartado, es producto del proyecto de investigación Caracterización, evaluación y multiplicación de Mnesithea selloana (Hack.) de Koning & Sosef (cola de lagarto, teyú ruguay) (PID UNER nº 2132, Resol. CS nº 327/08, 2008 - 2013) realizado en la Facultad de Ciencias
Agropecuarias Universidad Nacional de Entre Ríos, República Argentina. Los estudios fueron abordados en cuatro tramos generales, con trayectos específicos en cada uno de ellos. Lo mismos fueron: colecta y cultivo de plantas, caracterización y evaluación, cosecha, almacenamiento y conservación.
Cada tramo tuvo su metodología específica, detallada en los trabajos publicados. |
Palabras claves : |
ANATOMÍA FOLIAR; CAMPO NATURAL; PASTURAS. |
Asunto categoría : |
A50 Investigación agraria |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/14905/1/st-256-2020p8.15.pdf
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Marc : |
LEADER 01397naa a2200205 a 4500 001 1061626 005 2020-12-22 008 2020 bl uuuu u00u1 u #d 022 $a1688-9266 024 7 $a10.35676/INIA/ST.256$2DOI 100 1 $aGALUSSI, A. 245 $aAspectos botánicos y agronómiccos de Mnesithea Selloana (Hack) de Koning & Sosef (Cola de lagarto, Teyú Ruguay), Paraná, Entre Ríos, Repúbica Argentina. 260 $c2020 300 $ap. 8-15 490 $a(INIA Serie Técnica; 256). 520 $aLa información que se brinda en este apartado, es producto del proyecto de investigación Caracterización, evaluación y multiplicación de Mnesithea selloana (Hack.) de Koning & Sosef (cola de lagarto, teyú ruguay) (PID UNER nº 2132, Resol. CS nº 327/08, 2008 - 2013) realizado en la Facultad de Ciencias Agropecuarias Universidad Nacional de Entre Ríos, República Argentina. Los estudios fueron abordados en cuatro tramos generales, con trayectos específicos en cada uno de ellos. Lo mismos fueron: colecta y cultivo de plantas, caracterización y evaluación, cosecha, almacenamiento y conservación. Cada tramo tuvo su metodología específica, detallada en los trabajos publicados. 653 $aANATOMÍA FOLIAR 653 $aCAMPO NATURAL 653 $aPASTURAS 773 $tIn: Olmos, F. Ecofisiología de especies forrajeras del campo natural. Montevideo (UY): INIA, 2020.
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