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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
11/08/2016 |
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Actualizado : |
24/06/2021 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Nacionales |
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Autor : |
BRANDA, A.; FEDERICI, M.; DUTRA, F.; ROMERO, A.; BRIANO, C.; DALLA RIZZA, M.; LLAMBÍ, S. |
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Afiliación : |
ANDREA BRANDA SICA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARIA TERESA FEDERICI RODRIGUEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; F. DUTRA, MGAP/ DILAVE (División de Laboratorios de Veterinarios); A. ROMERO, MGAP/ DILAVE (División de Laboratorios de Veterinarios); C. BRIANO, MGAP/ DILAVE (División de Laboratorios de Veterinarios); MARCO DALLA RIZZA VILARO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; S. LLAMBÍ, Universidad de la República (UdelaR)/ Facultad de Veterinaria. |
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Título : |
Identificación de terneras Holando portadoras de BLAD y Citrulinemia en la región Este de Uruguay por PCR-RFLP y secuenciación. (Identification of Holstein heifer?s carriers BLAD and Citrullinemia in the eastern region of Uruguay by PCR-RFLP and sequencing). |
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Fecha de publicación : |
2016 |
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Fuente / Imprenta : |
Veterinaria (Montevideo), 2016, v. 52, no. 202, p. 23-27. |
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Idioma : |
Español |
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Notas : |
Recibido :11/5/2015. Aceptado: 28/8/2015 |
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Contenido : |
RESUMEN.
La deficiencia en la adhesión leucocitaria bovina (BLAD) y en la enzima arginosuccinato sintetasa (Citrulinemia) son enfermedades de herencia autosómica recesiva que han sido
descritas a nivel mundial en la raza Holando. El objetivo de este estudio fue optimizar e implementar una metodología de genotipado para la identificación de animales portadores
de los alelos causantes de estas enfermedades en una población cohorte de terneras de recría de la raza Holando de la cuenca lechera de Cerro Largo. Las muestras de ADN de 190 terneras Holando fueron extraídas a partir de sangre fresca, siguiendo normas y protocolos del Banco de ADN de la Unidad de Biotecnología INIA Las Brujas. El genotipado fue realizado mediante análisis PCR-RFLP con las enzimas de restricción TaqI para BLAD y Eco47I (AvaII) para Citrulinemia. La confirmación del genotipado fue evaluada mediante
secuenciación de los productos amplificados de ambas enfermedades. Se detectó la presencia del alelo mutante para BLAD en una sola ternera de recría y no se encontró portadoras
de Citrulinemia en la población analizada. Este trabajo representó el primer relevamiento de la prevalencia génica de las enfermedades BLAD y Citrulinemia en la región Este
del Uruguay.
ABSTRACT.
Deficiency in bovine leukocyte adhesion (BLAD) and arginosuccinate synthase enzyme (Citrullinemia) are autosomal recessive diseases that have been described worldwide in the
Holstein breed. The objective of this study was to optimize and implement a methodology of genotyping for the identification of animals carrying the allele causing these diseases in a population cohort of calves Holstein of dairy farm in Uruguay basin. DNA samples from 190 Holstein calves were extracted from fresh blood following rules and protocols DNA
Bank Unit Biotechnology INIA Las Brujas. Genotyping was performed by PCR-RFLP analysis with restriction enzymes TaqI for BLAD and Eco47I (AvaII) for Citrullinemia. Results
of RFLP genotyping were confirmed by sequencing of the amplified products of both diseases. The presence of the BLAD mutant allele was detected in only one calf while no
Citrullinemia carriers were found in the analyzed population. This study represented the first survey of the prevalence of BLAD and Citrullinemia diseases of dairy cattle in the eastern region of Uruguay. MenosRESUMEN.
La deficiencia en la adhesión leucocitaria bovina (BLAD) y en la enzima arginosuccinato sintetasa (Citrulinemia) son enfermedades de herencia autosómica recesiva que han sido
descritas a nivel mundial en la raza Holando. El objetivo de este estudio fue optimizar e implementar una metodología de genotipado para la identificación de animales portadores
de los alelos causantes de estas enfermedades en una población cohorte de terneras de recría de la raza Holando de la cuenca lechera de Cerro Largo. Las muestras de ADN de 190 terneras Holando fueron extraídas a partir de sangre fresca, siguiendo normas y protocolos del Banco de ADN de la Unidad de Biotecnología INIA Las Brujas. El genotipado fue realizado mediante análisis PCR-RFLP con las enzimas de restricción TaqI para BLAD y Eco47I (AvaII) para Citrulinemia. La confirmación del genotipado fue evaluada mediante
secuenciación de los productos amplificados de ambas enfermedades. Se detectó la presencia del alelo mutante para BLAD en una sola ternera de recría y no se encontró portadoras
de Citrulinemia en la población analizada. Este trabajo representó el primer relevamiento de la prevalencia génica de las enfermedades BLAD y Citrulinemia en la región Este
del Uruguay.
ABSTRACT.
Deficiency in bovine leukocyte adhesion (BLAD) and arginosuccinate synthase enzyme (Citrullinemia) are autosomal recessive diseases that have been described worldwide in the
Holstein breed. The objective of this study was to optimize and im... Presentar Todo |
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Palabras claves : |
CITRULLINEMIA; DAIRY CATTLE; GENOTYPING; LETAL HEREDITARY DISEASES. |
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Thesagro : |
BLAD; BOVINOS DE LECHE; CITRULINEMIA; ENFERMEDADES HEREDITARIAS LETALES; GENOTIPADO. |
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Asunto categoría : |
-- |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/5849/1/Branda-A.-2016.-SMVU.-v.52202-23-37.pdf
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Marc : |
LEADER 03430naa a2200313 a 4500
001 1055240
005 2021-06-24
008 2016 bl uuuu u00u1 u #d
100 1 $aBRANDA, A.
245 $aIdentificación de terneras Holando portadoras de BLAD y Citrulinemia en la región Este de Uruguay por PCR-RFLP y secuenciación. (Identification of Holstein heifer?s carriers BLAD and Citrullinemia in the eastern region of Uruguay by PCR-RFLP and sequencing).$h[electronic resource]
260 $c2016
500 $aRecibido :11/5/2015. Aceptado: 28/8/2015
520 $aRESUMEN. La deficiencia en la adhesión leucocitaria bovina (BLAD) y en la enzima arginosuccinato sintetasa (Citrulinemia) son enfermedades de herencia autosómica recesiva que han sido descritas a nivel mundial en la raza Holando. El objetivo de este estudio fue optimizar e implementar una metodología de genotipado para la identificación de animales portadores de los alelos causantes de estas enfermedades en una población cohorte de terneras de recría de la raza Holando de la cuenca lechera de Cerro Largo. Las muestras de ADN de 190 terneras Holando fueron extraídas a partir de sangre fresca, siguiendo normas y protocolos del Banco de ADN de la Unidad de Biotecnología INIA Las Brujas. El genotipado fue realizado mediante análisis PCR-RFLP con las enzimas de restricción TaqI para BLAD y Eco47I (AvaII) para Citrulinemia. La confirmación del genotipado fue evaluada mediante secuenciación de los productos amplificados de ambas enfermedades. Se detectó la presencia del alelo mutante para BLAD en una sola ternera de recría y no se encontró portadoras de Citrulinemia en la población analizada. Este trabajo representó el primer relevamiento de la prevalencia génica de las enfermedades BLAD y Citrulinemia en la región Este del Uruguay. ABSTRACT. Deficiency in bovine leukocyte adhesion (BLAD) and arginosuccinate synthase enzyme (Citrullinemia) are autosomal recessive diseases that have been described worldwide in the Holstein breed. The objective of this study was to optimize and implement a methodology of genotyping for the identification of animals carrying the allele causing these diseases in a population cohort of calves Holstein of dairy farm in Uruguay basin. DNA samples from 190 Holstein calves were extracted from fresh blood following rules and protocols DNA Bank Unit Biotechnology INIA Las Brujas. Genotyping was performed by PCR-RFLP analysis with restriction enzymes TaqI for BLAD and Eco47I (AvaII) for Citrullinemia. Results of RFLP genotyping were confirmed by sequencing of the amplified products of both diseases. The presence of the BLAD mutant allele was detected in only one calf while no Citrullinemia carriers were found in the analyzed population. This study represented the first survey of the prevalence of BLAD and Citrullinemia diseases of dairy cattle in the eastern region of Uruguay.
650 $aBLAD
650 $aBOVINOS DE LECHE
650 $aCITRULINEMIA
650 $aENFERMEDADES HEREDITARIAS LETALES
650 $aGENOTIPADO
653 $aCITRULLINEMIA
653 $aDAIRY CATTLE
653 $aGENOTYPING
653 $aLETAL HEREDITARY DISEASES
700 1 $aFEDERICI, M.
700 1 $aDUTRA, F.
700 1 $aROMERO, A.
700 1 $aBRIANO, C.
700 1 $aDALLA RIZZA, M.
700 1 $aLLAMBÍ, S.
773 $tVeterinaria (Montevideo), 2016$gv. 52, no. 202, p. 23-27.
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Registro original : |
INIA Las Brujas (LB) |
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 | Acceso al texto completo restringido a Biblioteca INIA Tacuarembó. Por información adicional contacte bibliotb@tb.inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Tacuarembó; INIA Treinta y Tres. |
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Fecha actual : |
10/06/2019 |
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Actualizado : |
06/07/2020 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
CARRACELAS, G.; HORNBUCKLE, J.; ROSAS, J.E.; ROEL, A. |
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Afiliación : |
JULIO GONZALO CARRACELAS GARRIDO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. Deakin University, Faculty of Science Engineering & Built Environment, Centre for Regional and Rural Futures, Griffith, New South Wales, Australia.; J. HORNBUCKLE, Deakin University, Faculty of Science Engineering & Built Environment, Centre for Regional and Rural Futures, Griffith, New South Wales, Australia.; JUAN EDUARDO ROSAS CAISSIOLS, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ALVARO ROEL DELLAZOPPA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Irrigation management strategies to increase water productivity in Oryza sativa (rice) in Uruguay. |
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Fecha de publicación : |
2019 |
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Fuente / Imprenta : |
Agricultural Water Management, Volume 222, 1 August 2019, Pages 161-172. DOI: https://doi.org/10.1016/j.agwat.2019.05.049 |
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DOI : |
10.1016/j.agwat.2019.05.049 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 29 March 2019; Received in revised form 29 May 2019; Accepted 30 May 2019. |
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Contenido : |
Traditional rice irrigation systems in Uruguay are fully irrigated and early continuously flooded irrigation accounts for a high volume of water used. The purpose of this study was to determine irrigation techniques that increase irrigation water productivity (WPi) allowing a reduction in water input without negatively affecting grain yield in Uruguay. Ten experiments were conducted over a six-year period from 2009 to 2015, in three experimental units located among the major rice growing regions. Treatments included: early continuous flooding (C), alternate wetting and drying (AWD), intermittent flooding until panicle initiation (IP) and intermittent flooding during all crop growth period (I). All treatments were planted on dry soil. In treatment C flooding started 15?20 days after emergence and a water layer of 10 cm above the soil surface was maintained throughout all the crop cycle. In treatments IP and I, the water level alternated between 10 cm and 0 cm and was re-established when the soil was still saturated. The AWD treatment allowed the soil to dry periodically (water
depletion of 50% of soil available water) until panicle initiation. IP and I over three seasons led to significant savings in irrigation water inputs in the North and Central regions (averaged 35% or - 3986 m3 ha−1) in relation to C. In the East region, AWD allowed for a 29%(-2067m3 ha−1) water saving in relation to the control over four seasons but determined a significant yield loss of 1339 kg rice ha−1 (15% reduction) in relation to C. WPi was increased by 0.25 kg m−3 (23%) in IP and 0.68 kg m−3 (62%) in I, in relation to the control C. Whole grain percentage was significantly reduced with I in the North region only. Techniques that maintained the soil water at saturated conditions like intermittent flooding, allowed a reduction of water input with no significant effects
on grain yield, which led to a significant increase in WPi. MenosTraditional rice irrigation systems in Uruguay are fully irrigated and early continuously flooded irrigation accounts for a high volume of water used. The purpose of this study was to determine irrigation techniques that increase irrigation water productivity (WPi) allowing a reduction in water input without negatively affecting grain yield in Uruguay. Ten experiments were conducted over a six-year period from 2009 to 2015, in three experimental units located among the major rice growing regions. Treatments included: early continuous flooding (C), alternate wetting and drying (AWD), intermittent flooding until panicle initiation (IP) and intermittent flooding during all crop growth period (I). All treatments were planted on dry soil. In treatment C flooding started 15?20 days after emergence and a water layer of 10 cm above the soil surface was maintained throughout all the crop cycle. In treatments IP and I, the water level alternated between 10 cm and 0 cm and was re-established when the soil was still saturated. The AWD treatment allowed the soil to dry periodically (water
depletion of 50% of soil available water) until panicle initiation. IP and I over three seasons led to significant savings in irrigation water inputs in the North and Central regions (averaged 35% or - 3986 m3 ha−1) in relation to C. In the East region, AWD allowed for a 29%(-2067m3 ha−1) water saving in relation to the control over four seasons but determined a significant yield loss of 133... Presentar Todo |
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Palabras claves : |
ALTERNATE WETTING AND DRYING; INTERMITTENT; QUALITY; RICE (ORYZA SATIVA L.); WATER PRODUCTIVITY; YIELD. |
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Thesagro : |
ARROZ. |
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Asunto categoría : |
F01 Cultivo |
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Marc : |
LEADER 02901naa a2200265 a 4500
001 1059837
005 2020-07-06
008 2019 bl uuuu u00u1 u #d
024 7 $a10.1016/j.agwat.2019.05.049$2DOI
100 1 $aCARRACELAS, G.
245 $aIrrigation management strategies to increase water productivity in Oryza sativa (rice) in Uruguay.$h[electronic resource]
260 $c2019
500 $aArticle history: Received 29 March 2019; Received in revised form 29 May 2019; Accepted 30 May 2019.
520 $aTraditional rice irrigation systems in Uruguay are fully irrigated and early continuously flooded irrigation accounts for a high volume of water used. The purpose of this study was to determine irrigation techniques that increase irrigation water productivity (WPi) allowing a reduction in water input without negatively affecting grain yield in Uruguay. Ten experiments were conducted over a six-year period from 2009 to 2015, in three experimental units located among the major rice growing regions. Treatments included: early continuous flooding (C), alternate wetting and drying (AWD), intermittent flooding until panicle initiation (IP) and intermittent flooding during all crop growth period (I). All treatments were planted on dry soil. In treatment C flooding started 15?20 days after emergence and a water layer of 10 cm above the soil surface was maintained throughout all the crop cycle. In treatments IP and I, the water level alternated between 10 cm and 0 cm and was re-established when the soil was still saturated. The AWD treatment allowed the soil to dry periodically (water depletion of 50% of soil available water) until panicle initiation. IP and I over three seasons led to significant savings in irrigation water inputs in the North and Central regions (averaged 35% or - 3986 m3 ha−1) in relation to C. In the East region, AWD allowed for a 29%(-2067m3 ha−1) water saving in relation to the control over four seasons but determined a significant yield loss of 1339 kg rice ha−1 (15% reduction) in relation to C. WPi was increased by 0.25 kg m−3 (23%) in IP and 0.68 kg m−3 (62%) in I, in relation to the control C. Whole grain percentage was significantly reduced with I in the North region only. Techniques that maintained the soil water at saturated conditions like intermittent flooding, allowed a reduction of water input with no significant effects on grain yield, which led to a significant increase in WPi.
650 $aARROZ
653 $aALTERNATE WETTING AND DRYING
653 $aINTERMITTENT
653 $aQUALITY
653 $aRICE (ORYZA SATIVA L.)
653 $aWATER PRODUCTIVITY
653 $aYIELD
700 1 $aHORNBUCKLE, J.
700 1 $aROSAS, J.E.
700 1 $aROEL, A.
773 $tAgricultural Water Management, Volume 222, 1 August 2019, Pages 161-172. DOI: https://doi.org/10.1016/j.agwat.2019.05.049
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