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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
Fecha : |
29/07/2022 |
Actualizado : |
02/09/2022 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
GIANNITTI, F.; ARÁOZ, V.; SILVEIRA, C.S.; FRANCIA, M.E.; ROBELLO, C.; CABRERA, A. |
Afiliación : |
FEDERICO GIANNITTI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; VIRGINIA ARÁOZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CAROLINE DA SILVA SILVEIRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARÍA E. FRANCIA, Laboratorio de Biología de Apicomplejos, Institut Pasteur de Montevideo, Montevideo, Uruguay; Departamento de Parasitología y Micología, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay.; CARLOS ROBELLO, Laboratorio de Interacciones Hospedero-Patógeno, Instituto Pasteur de Montevideo, Montevideo, Uruguay; Departamento de Bioquímica, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay.; ANDRÉS CABRERA, Laboratorio de Interacciones Hospedero-Patógeno, Instituto Pasteur de Montevideo, Montevideo, Uruguay; Unidad de Microbiología, Departamento de Patobiología, Facultad de Veterinaria, Universidad de la República, Montevideo, Uruguay. |
Título : |
A Holstein heifer infected with Neospora caninum NcUru3 congenitally transmits this strain to a viable offspring although infection does not protect her from aborting by a different N. caninum genotype in the subsequent gestation. |
Fecha de publicación : |
2022 |
Fuente / Imprenta : |
Frontiers in Veterinary Science, 2022; 9: 889157. OPEN ACCESS. Doi: https://doi.org/10.3389/fvets.2022.889157 |
DOI : |
10.3389/fvets.2022.889157 |
Idioma : |
Inglés |
Notas : |
Article history: Received 03 March 2022/Accepted 05 July 2022/Published 25 July 2022. |
Contenido : |
Abstract: Neospora caninum is a leading cause of bovine abortion worldwide. Although the genetic diversity of this apicomplexan parasite has long been recognized, there is little information on whether infection with different genotypes results in different clinical outcomes or whether infection by a given genotype impairs protective immunity against abortion induced by different genotypes. Here, we provide evidence supporting that natural subclinical infection with isolate NcUru3 of N. caninum in a pregnant heifer did not provide protection against abortion caused by a different N. caninum genotype in the subsequent gestation. A Holstein heifer delivered a healthy calf congenitally infected with N. caninum. Specific anti-N. caninum IgG was detected by indirect ELISA in sera obtained from the dam at calving and the calf before ingestion of colostrum, indicating in utero exposure to the parasite in the latter. A N. caninum strain named NcUru3 was isolated and characterized by multilocus microsatellite typing from the brain of this neonate euthanized at 9 days of age. Sixty days after calving, the cow got pregnant, although she aborted spontaneously at ~6 months of gestation. Pathologic examination of the aborted fetus and placenta revealed typical lesions of neosporosis, including encephalitis, myocarditis, hepatitis, myositis, and placentitis. Neospora caninum DNA was amplified from the fetal brain, heart, kidney, and placenta, and multilocus microsatellite typing revealed a genotype that differed from isolate NcUru3 at the level of microsatellite marker 6A (MS6A). Serum obtained from the dam at the time of abortion had IgG that cross-recognized isolate NcUru3, as demonstrated by immunoblotting, indicating that the humoral immune response did not prevent the other genotype from infecting the fetus and inducing fetoplacental lesions and abortion. This is the first description of one same dam transmitting two N. caninum genotypes to her offspring in subsequent gestations. MenosAbstract: Neospora caninum is a leading cause of bovine abortion worldwide. Although the genetic diversity of this apicomplexan parasite has long been recognized, there is little information on whether infection with different genotypes results in different clinical outcomes or whether infection by a given genotype impairs protective immunity against abortion induced by different genotypes. Here, we provide evidence supporting that natural subclinical infection with isolate NcUru3 of N. caninum in a pregnant heifer did not provide protection against abortion caused by a different N. caninum genotype in the subsequent gestation. A Holstein heifer delivered a healthy calf congenitally infected with N. caninum. Specific anti-N. caninum IgG was detected by indirect ELISA in sera obtained from the dam at calving and the calf before ingestion of colostrum, indicating in utero exposure to the parasite in the latter. A N. caninum strain named NcUru3 was isolated and characterized by multilocus microsatellite typing from the brain of this neonate euthanized at 9 days of age. Sixty days after calving, the cow got pregnant, although she aborted spontaneously at ~6 months of gestation. Pathologic examination of the aborted fetus and placenta revealed typical lesions of neosporosis, including encephalitis, myocarditis, hepatitis, myositis, and placentitis. Neospora caninum DNA was amplified from the fetal brain, heart, kidney, and placenta, and multilocus microsatellite typing revealed a... Presentar Todo |
Palabras claves : |
ABORTION; DAIRY CATTLE; GENETIC DIVERSITY; Molecular epidemiology; Multilocus microsatellite typin; Neospora caninum; PATHOLOGY; PLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL; PLATAFORMA DE SALUD ANIMAL; REPRODUCTIVE DISEASES. |
Asunto categoría : |
-- |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/16659/1/fvets-09-889157.pdf
https://www.frontiersin.org/articles/10.3389/fvets.2022.889157/full
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Marc : |
LEADER 03260naa a2200325 a 4500 001 1063470 005 2022-09-02 008 2022 bl uuuu u00u1 u #d 024 7 $a10.3389/fvets.2022.889157$2DOI 100 1 $aGIANNITTI, F. 245 $aA Holstein heifer infected with Neospora caninum NcUru3 congenitally transmits this strain to a viable offspring although infection does not protect her from aborting by a different N. caninum genotype in the subsequent gestation.$h[electronic resource] 260 $c2022 500 $aArticle history: Received 03 March 2022/Accepted 05 July 2022/Published 25 July 2022. 520 $aAbstract: Neospora caninum is a leading cause of bovine abortion worldwide. Although the genetic diversity of this apicomplexan parasite has long been recognized, there is little information on whether infection with different genotypes results in different clinical outcomes or whether infection by a given genotype impairs protective immunity against abortion induced by different genotypes. Here, we provide evidence supporting that natural subclinical infection with isolate NcUru3 of N. caninum in a pregnant heifer did not provide protection against abortion caused by a different N. caninum genotype in the subsequent gestation. A Holstein heifer delivered a healthy calf congenitally infected with N. caninum. Specific anti-N. caninum IgG was detected by indirect ELISA in sera obtained from the dam at calving and the calf before ingestion of colostrum, indicating in utero exposure to the parasite in the latter. A N. caninum strain named NcUru3 was isolated and characterized by multilocus microsatellite typing from the brain of this neonate euthanized at 9 days of age. Sixty days after calving, the cow got pregnant, although she aborted spontaneously at ~6 months of gestation. Pathologic examination of the aborted fetus and placenta revealed typical lesions of neosporosis, including encephalitis, myocarditis, hepatitis, myositis, and placentitis. Neospora caninum DNA was amplified from the fetal brain, heart, kidney, and placenta, and multilocus microsatellite typing revealed a genotype that differed from isolate NcUru3 at the level of microsatellite marker 6A (MS6A). Serum obtained from the dam at the time of abortion had IgG that cross-recognized isolate NcUru3, as demonstrated by immunoblotting, indicating that the humoral immune response did not prevent the other genotype from infecting the fetus and inducing fetoplacental lesions and abortion. This is the first description of one same dam transmitting two N. caninum genotypes to her offspring in subsequent gestations. 653 $aABORTION 653 $aDAIRY CATTLE 653 $aGENETIC DIVERSITY 653 $aMolecular epidemiology 653 $aMultilocus microsatellite typin 653 $aNeospora caninum 653 $aPATHOLOGY 653 $aPLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL 653 $aPLATAFORMA DE SALUD ANIMAL 653 $aREPRODUCTIVE DISEASES 700 1 $aARÁOZ, V. 700 1 $aSILVEIRA, C.S. 700 1 $aFRANCIA, M.E. 700 1 $aROBELLO, C. 700 1 $aCABRERA, A. 773 $tFrontiers in Veterinary Science, 2022; 9: 889157. OPEN ACCESS. Doi: https://doi.org/10.3389/fvets.2022.889157
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82. | | MAURENTE BERÓN, M.; FERNÁNDEZ-CIGANDA, S.; GIANNITTI, F.; FRAGA, M. Comparison of PCR and qPCR for the detection of Mycobacterium avium subsp. paratuberculosis in feces and milk of dairy cows in Uruguay: a longitudinal study. (ID-P47). [poster-Infectious Diseases] Session: Posters. In: World Buiatrics Congress (WBC), 31 st., Abstract Book, Madrid, Spain, September 4-8, 2022.Biblioteca(s): INIA Las Brujas. |
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83. | | PELOCHE, D.; COURDIN, V.; CIDADE, G.; GONZÁLEZ, E.; MALARINI, F.; MAS, L.; MORAN, E.; PEREYRA, M.; SANCHIS, M.; ALVAREZ, S. Cultivos de servicio: características de su adopción en los sistemas agrícolas. Proyectos FPTA. Revista INIA Uruguay, Setiembre 2022, no.70, p.97-101. (Revista INIA; 70).Tipo: Artículos en Revistas Agropecuarias |
Biblioteca(s): INIA Las Brujas. |
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84. | | MACHADO, M.; RIET-CORREA, F.; PEDROSO, P.M.O.; MACÊDO, J.T.S.A. Doramectin intoxication in malnourished 15-month-old cattle. [Intoxicação por doramectina em bovinos malnutridos de 15 meses de idade.] Pesquisa Veterinaria Brasileira, 2022, Volume 42, Article e07102. OPEN ACCESS. doi: https://doi.org/10.1590/1678-5150-PVB-7102 Article history: Received 15 May 2022; Accepted 31 May 2022; Publication 29 July 2022; Date of issue 2022.
Corresponding author: Riet-Correa, F.; Animal Sciences in the Tropics (PPGCAT), Faculdade de Medicina Veterinária e Zootecnia,...Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
Biblioteca(s): INIA Las Brujas. |
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85. | | FABIUS, S.; URBANAVICIUS, J.; FERNÁNDEZ-CIGANDA, S.; PRIETO, J.P.; LOZANO, J.; PICCINI, C.; ZUNINO, P.; SCORZA, C. Efecto del pretratamiento oral con Lactobacillus SPP. en la sensibilización locomotora inducida por cocaína y su influencia en la microbiota intestinal. [resumen] Conferencia 3. Mesa Sociedad de Neurociencias del Uruguay. Mesa VI. Presentaciones orales seleccionadas de estudiantes. In: Physiological Mini Reviews, 2022, volume 15, Special Issue: III (3er) Congreso Nacional de Biociencias Octubre 2022, Montevideo, Uruguay. p.38. Resumen publicado en las jornadas de BIOCIENCIAS: II Jornadas Binacionales Argentina-Uruguay; III Congreso Nacional 2022 "Ciencia para el desarrollo sustentable".Tipo: Abstracts/Resúmenes |
Biblioteca(s): INIA Las Brujas. |
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86. | | LOZANO, J.; FERNÁNDEZ-CIGANDA, S.; FABIUS, S.; PICCINI, C.; SCORZA, C.; ZUNINO, P. Efecto tipo-antidepresivo inducido por el tratamiento crónico de una cepa del grupo lactobacillus plantarum en ratas y asociación con la microbiota intestinal. 395. (resúmen) Conferencia 21. Mesa Área Biotecnología. Organiza SUM (Sociedad Uruguaya de Microbiología). In: Physiological Mini Reviews, 2022, volume 15, Special Issue: III (3er) Congreso Nacional de Biociencias Octubre 2022, Montevideo, Uruguay. p.86. Resumen publicado en las jornadas de BIOCIENCIAS: II Jornadas Binacionales Argentina-Uruguay; III Congreso Nacional 2022 "Ciencia para el desarrollo sustentable".Tipo: Abstracts/Resúmenes |
Biblioteca(s): INIA Las Brujas. |
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87. | | CIUFFO, C.; ARÉVALO, A.P.; GARCIA, F.; PERDOMO, T.; CRISPO, M.; GIANNITTI, F.; BUSCHIAZZO, A.; ZARANTONELLI, L. Estudio y caracterización de una variante autóctona de Leptospira para su potencial uso como antígeno en vacunas de uso veterinario. In: Jornadas Uruguayas de Buiatría, 49., 2022. Paysandú, Uruguay: Centro Médico Veterinario de Paysandú; Filial de la Sociedad de Medicina Veterinaria del Uruguaya; Sociedad Uruguaya de Buiatría, 9 al 11 Junio 2022. p.192-194. p.192-194.Tipo: Trabajos en Congresos/Conferencias |
Biblioteca(s): INIA Las Brujas. |
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88. | | DONCEL, B.; CASTELLS, M.; MAYA, L.; FRAGA, M.; UZAL, F.A; COLINA, R.; GIANNITTI, F. Encefalitis asociada a astrovirus bovino neurotrópico, ¿una enfermedad subdiagnosticada en Sudamérica?.[Neurotropic bovine astrovirus-associated encephalitis: An underdiagnosed disease in South America?]. Revista Argentina de Microbiologia, Volume 54, Pages 100-105, 2022. OPEN ACCESS. Doi: https://doi.org/10.1016/j.ram.2021.01.00 Article history: Recibido el 15 de julio de 2020/ Aaceptado el 24 de enero de 2021./Disponible en Internet el 18 junio 2021. Autor para correspondencia: Correo electrónico: fgiannitti@inia.org.uy (F. Giannitti). Este trabajo fue...Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
Biblioteca(s): INIA La Estanzuela. |
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89. | | ARAGUNDE VIEYTES, R.; VIÑOLES, C.; GASTAL, G.D.A.; CAVESTANY, D. Equine chorionic gonadotropin administered on day 5 of a 7-days fixed-time artificial insemination program improves ovulation synchrony and corpus luteum function in anestrous beef cows Theriogenology, January 2023, Volume 195, Pages 62-68. doi: https://doi.org/10.1016/j.theriogenology.2022.10.016 Article history: Received 17 June 2022; Received in revised form 5 October 2022; Accepted 12 October 2022; Available online 17 October 2022.
Correspondence authors: Gastal, G.D.A.; Ruta 11, Km 50, Colonia del Sacramento, Colonia,...Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
Biblioteca(s): INIA Las Brujas. |
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92. | | BUSCHIAZZO, M.; MERINO, N.; DE MORI, J.; PASSADORE, A.; VILLARINO, A.; BERTOLA, B.; CONIBERTI, A.; FASIOLO, C. FPTA 353: Uruguay inicia el camino a la certificación de una vitivinicultura sostenible. Proyectos FPTA. Revista INIA Uruguay, Junio 2022, no.69, p.106-108. (Revista INIA; 69).Tipo: Artículos en Revistas Agropecuarias |
Biblioteca(s): INIA Las Brujas. |
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93. | | RIVERO CAYETANO, J.; RAMOS, J.; LUCAS, J.; GARCÍA PINTOS, J.; GARCÍA PINTOS, M.; AGUERRE, J.; ORIHUELA, M.; FERREIRA, G.; FIERRO, S.; SALADA, S. FPTA 360: sostenibilidad del ovino en sistemas ganaderos mixtos. Proyectos FPTA. Revista INIA Uruguay, Junio 2022, no.69, p.109-112. (Revista INIA; 69).Tipo: Artículos en Revistas Agropecuarias |
Biblioteca(s): INIA Las Brujas. |
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94. | | CUADRO, F.; DE BRUN, V.; BROCHADO, C.; SOUZA, M.; GARCÍA PINTOS, C.; MENEZES, C.; GASTAL, G.D.A.; MENCHACA, A. Gene expression patterns in uterus and oviduct during the preovulatory period in ewes. In: 19th International Congress on Animal Reproduction (ICAR), Bologna (Italy), 26-30 June 2022. p.185.Biblioteca(s): INIA La Estanzuela. |
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95. | | CLOTHIER, K.A.; WATSON, K.D.; METE, A.; GIANNITTI, F.; ANDERSON, M.; MUNK, B.; MCMILLIN, S.; CLIFFORD, D.L.; RUDD, J.; SHIRKEY, N.; FAMINI, D.; WOODS, L. Generalized dermatophytosis caused by Trichophyton equinum in 8 juvenile black bears in California. Journal of Veterinary Diagnostic Investigation, 2022, Volume 34, Issue 2, pp.279-283. doi: https://doi.org/10.1177/10406387211061143Biblioteca(s): INIA La Estanzuela. |
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99. | | MENEGASSI, S.R. DE O.; PEREIRA, G.R.; ROCHA, M.K.DA; KOETZ JR., C.; LOPEZ, L.F.D.; AUGUST, P.M.; MATTÉ, C.; GOMES, M.G.T.; DAL BEM, J. DE C.T.; CANOZZI, M.E.A.; BARCELLOS, J.O.J. Influence of tropical, subtropical, and temperate climate conditions on seminal redox status and sperm quality parameters in breeding bulls. Journal of Thermal Biology, October 2022, Volume 109, Article number 103319. doi: https://doi.org/10.1016/j.jtherbio.2022.103319 12 p.Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
Biblioteca(s): INIA Treinta y Tres. |
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