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Biblioteca (s) : |
INIA La Estanzuela; INIA Las Brujas; INIA Treinta y Tres. |
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Fecha : |
14/02/2022 |
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Actualizado : |
14/03/2022 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
GRAHMANN, K.; TERRA, J.A.; ELLERBROCK, R.; RUBIO, V.; BARRO, R.; CAMAÑO, A.; QUINCKE, A. |
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Afiliación : |
KATHRIN GRAHMANN, Leibniz Centre for Agricultural Landscape Research (ZALF); Eberswalder Str.84, 15374 Müncheberg, Germany; JOSÉ ALFREDO TERRA FERNÁNDEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RUTH ELLERBROCK, Leibniz Centre for Agricultural Landscape Research (ZALF); Eberswalder Str.84, 15374 Müncheberg; VALENTINA RUBIO DELLEPIANE, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RAQUEL BARRO, Postdoctoral Scholarship ANII (National agency of innovation and research, Uruguay); ALEJANDRO CAMAÑO, Service Unit for the Evaluation of Quality and Environmental Control, Department of Environmental Development, Intendancy of Montevideo, Uruguay; JUAN ANDRES QUINCKE WALDEN, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Data accuracy and method validation of chemical soil properties in long-term experiments: Standard operating procedures for a non-certified soil laboratory in Latin America. |
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Fecha de publicación : |
2022 |
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Fuente / Imprenta : |
Geoderma Regional, 2022. Volume 28, Article number e00487. OPEN ACCESS. doi: https://doi.org/10.1016/j.geodrs.2022.e00487 |
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DOI : |
10.1016/j.geodrs.2022.e00487 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 9 May 2021, Revised 20 December 2021, Accepted 2 February 2022, Available online 6 February 2022. |
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Contenido : |
ABSTRACT.- Long-term agricultural experiments (LTE) are essential to detect cumulative treatment effects on soil properties and to design sustainable production systems. However, to ensure high quality of long-term soil data and their correct interpretation, several analytical issues regarding the accuracy and analytical laboratory bias need to be considered. This paper aims to (1) evaluate laboratory precision and trueness of analytical soil data for the evaluation of long-term trends in LTE and to (2) assess the limitations and challenges for non-certified soil laboratories that might compromise the quality of analytical soil data. A data set of internal reference soil materials (IRM) collected over 16?years and interlaboratory data from eleven years were analyzed to verify method precision, trueness, and the subsequent long-term dataset reliability for several soil quality parameters: organic carbon (SOC, determined either by wet or dry combustion), pH (water), extractable phosphorous (either Bray I or citric acid; Bray-P or citric acid-P), and exchangeable potassium (Kexch). Results showed that IRM used by the laboratory were homogenous in terms of physical and chemical composition and appropriate to confirm the precision of long-term soil survey data. The relative standard deviation for repeatability and reproducibility (RSDR) ranged from 1.5% for SOC by wet combustion to 9.5% for citric acid-P. HorRat values (the ratio of the estimated standard deviations of reproducibility and the repeatability found for individual analytical procedures) for all chemical soil properties were within the acceptable ranges of <2.0. Interlaboratory trials for soil pH and SOC showed tolerable standard Z-Scores under 2.0 (Z-Score, calculated from the difference between laboratory results and the assigned value divided by the standard deviation), verifying the trueness of data. The results of this study reinforce the validity of analytical soil data originating from the non-certified laboratory in Uruguay obtaining both precise and true soil quality data over a long period of time for most soil analytes. Nevertheless, the analytical flaws in LTE soil monitoring were revealed and can only be minimized in ongoing and future studies through the inclusion of certified reference material. These recommendations should guide future research activities in LTE studies on analytical data quality management as a requirement for long-term soil monitoring. Finally, the paper proposes a proficiency testing procedure for soil laboratories to achieve and maintain high analytical quality for LTE soil research.
© 2022 The Authors. Published by Elsevier B.V. MenosABSTRACT.- Long-term agricultural experiments (LTE) are essential to detect cumulative treatment effects on soil properties and to design sustainable production systems. However, to ensure high quality of long-term soil data and their correct interpretation, several analytical issues regarding the accuracy and analytical laboratory bias need to be considered. This paper aims to (1) evaluate laboratory precision and trueness of analytical soil data for the evaluation of long-term trends in LTE and to (2) assess the limitations and challenges for non-certified soil laboratories that might compromise the quality of analytical soil data. A data set of internal reference soil materials (IRM) collected over 16?years and interlaboratory data from eleven years were analyzed to verify method precision, trueness, and the subsequent long-term dataset reliability for several soil quality parameters: organic carbon (SOC, determined either by wet or dry combustion), pH (water), extractable phosphorous (either Bray I or citric acid; Bray-P or citric acid-P), and exchangeable potassium (Kexch). Results showed that IRM used by the laboratory were homogenous in terms of physical and chemical composition and appropriate to confirm the precision of long-term soil survey data. The relative standard deviation for repeatability and reproducibility (RSDR) ranged from 1.5% for SOC by wet combustion to 9.5% for citric acid-P. HorRat values (the ratio of the estimated standard deviations of reproducib... Presentar Todo |
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Palabras claves : |
Data precision; Internal reference material; Repeatability; Reproducibility; Soil organic carbon; Trueness; URUGUAY. |
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Asunto categoría : |
P30 Ciencia del suelo y manejo del suelo |
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URL : |
https://www.sciencedirect.com/science/article/pii/S2352009422000074/pdf?md5=8d5a81ff8de54639cfdb18c6fdec1c4a&pid=1-s2.0-S2352009422000074-main.pdf
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Marc : |
LEADER 03790naa a2200301 a 4500
001 1062741
005 2022-03-14
008 2022 bl uuuu u00u1 u #d
024 7 $a10.1016/j.geodrs.2022.e00487$2DOI
100 1 $aGRAHMANN, K.
245 $aData accuracy and method validation of chemical soil properties in long-term experiments$bStandard operating procedures for a non-certified soil laboratory in Latin America.$h[electronic resource]
260 $c2022
500 $aArticle history: Received 9 May 2021, Revised 20 December 2021, Accepted 2 February 2022, Available online 6 February 2022.
520 $aABSTRACT.- Long-term agricultural experiments (LTE) are essential to detect cumulative treatment effects on soil properties and to design sustainable production systems. However, to ensure high quality of long-term soil data and their correct interpretation, several analytical issues regarding the accuracy and analytical laboratory bias need to be considered. This paper aims to (1) evaluate laboratory precision and trueness of analytical soil data for the evaluation of long-term trends in LTE and to (2) assess the limitations and challenges for non-certified soil laboratories that might compromise the quality of analytical soil data. A data set of internal reference soil materials (IRM) collected over 16?years and interlaboratory data from eleven years were analyzed to verify method precision, trueness, and the subsequent long-term dataset reliability for several soil quality parameters: organic carbon (SOC, determined either by wet or dry combustion), pH (water), extractable phosphorous (either Bray I or citric acid; Bray-P or citric acid-P), and exchangeable potassium (Kexch). Results showed that IRM used by the laboratory were homogenous in terms of physical and chemical composition and appropriate to confirm the precision of long-term soil survey data. The relative standard deviation for repeatability and reproducibility (RSDR) ranged from 1.5% for SOC by wet combustion to 9.5% for citric acid-P. HorRat values (the ratio of the estimated standard deviations of reproducibility and the repeatability found for individual analytical procedures) for all chemical soil properties were within the acceptable ranges of <2.0. Interlaboratory trials for soil pH and SOC showed tolerable standard Z-Scores under 2.0 (Z-Score, calculated from the difference between laboratory results and the assigned value divided by the standard deviation), verifying the trueness of data. The results of this study reinforce the validity of analytical soil data originating from the non-certified laboratory in Uruguay obtaining both precise and true soil quality data over a long period of time for most soil analytes. Nevertheless, the analytical flaws in LTE soil monitoring were revealed and can only be minimized in ongoing and future studies through the inclusion of certified reference material. These recommendations should guide future research activities in LTE studies on analytical data quality management as a requirement for long-term soil monitoring. Finally, the paper proposes a proficiency testing procedure for soil laboratories to achieve and maintain high analytical quality for LTE soil research. © 2022 The Authors. Published by Elsevier B.V.
653 $aData precision
653 $aInternal reference material
653 $aRepeatability
653 $aReproducibility
653 $aSoil organic carbon
653 $aTrueness
653 $aURUGUAY
700 1 $aTERRA, J.A.
700 1 $aELLERBROCK, R.
700 1 $aRUBIO, V.
700 1 $aBARRO, R.
700 1 $aCAMAÑO, A.
700 1 $aQUINCKE, A.
773 $tGeoderma Regional, 2022. Volume 28, Article number e00487. OPEN ACCESS. doi: https://doi.org/10.1016/j.geodrs.2022.e00487
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Registro original : |
INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA Tacuarembó. |
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Fecha actual : |
11/09/2014 |
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Actualizado : |
30/09/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Nacionales |
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Circulación / Nivel : |
Nacional - -- |
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Autor : |
BOVE, R.; LÓPEZ, F.; PERERA, C.; CARRACELAS, B.; TORRES, D.; DE SOUZA, G.; AZAMBUJA, C.; BERMÚDEZ, J.; ALZUGARAY, F.; MEDEROS, A. |
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Afiliación : |
DILAVE.; DILAVE.; DILAVE.; EMERITA BEATRIZ CARRACELAS MARQUEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; DIEGO GABRIEL TORRES DINI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; GUILLERMO DE SOUZA CAMARGO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; Laboratorio Genia, Montevideo, Uruguay.; Facultad de Veterinaria, UdelaR, Uruguay.; Facultad de Veterinaria, UdelaR, Uruguay.; AMERICA ESTHER MEDEROS SILVEIRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Diagnóstico Campylobacter fetus venerealis por PCR, en un aborto bovino espontáneo // Diagnosis of Campylobacter fetus venerealis in aborted bovine fetus. |
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Fecha de publicación : |
2013 |
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Fuente / Imprenta : |
Veterinaria (Montevideo), 2013, v. 49, no. 192, p. 20-28. |
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Idioma : |
Español |
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Notas : |
History article: Recibido: 25/02/2013; Aprobado: 01/04/2013. |
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Contenido : |
En este trabajo se reporta el diagnóstico de Campylobacter fetus venerealis (Cfv) de un aborto bovino espontáneo utilizando la técnica de reacción en cadena de la polimerasa (PCR) múltiple. La muestra de fluido estomacal fetal fue sometida a otros métodos de diagnóstico para Campylobacter fetus: cultivo bacteriano e inmunoflurescencia directa (IFD). Adicionalmente se emplearon marcadores moleculares específicos de CFv que permitieron identificar una banda de 142pb asociada al gen plasmídico parA Cfv. Dicho amplicón fue secuenciado y analizado por blastN. Los resultados de los cultivos bacteriológicos e IFD evidenciaron la presencia de Campylobacter fetus y los resultados moleculares identificaron la subespecie venerealis. El resultado de la secuenciación reveló un e-value de 3e-33 con un valor de identidad del 99%, confirmando la subespecie venerealis // The aim of this study was to report the diagnosis of Campylobacter fetus venerealis (Cfv) in a bovine fetus by a multiplex Polimerase chain reaction test (PCR). The sample was submitted to the following diagnostic tests: bacteriological culture and direct immunofluorescence test (DIFT) and specific molecular markers were used for identification of a 142bp segment associated to the Cfv parA plasmid gene. This segment was sequenced and analyzed with BlastN. Results from the bacteriological tests and DIFT demonstrated Campylobacter fetus presence, while the molecular methods confirmed Cfv. Sequencing results revealed a 3e-33 e-value with a 99% identity, confirming subspecies venerealis. MenosEn este trabajo se reporta el diagnóstico de Campylobacter fetus venerealis (Cfv) de un aborto bovino espontáneo utilizando la técnica de reacción en cadena de la polimerasa (PCR) múltiple. La muestra de fluido estomacal fetal fue sometida a otros métodos de diagnóstico para Campylobacter fetus: cultivo bacteriano e inmunoflurescencia directa (IFD). Adicionalmente se emplearon marcadores moleculares específicos de CFv que permitieron identificar una banda de 142pb asociada al gen plasmídico parA Cfv. Dicho amplicón fue secuenciado y analizado por blastN. Los resultados de los cultivos bacteriológicos e IFD evidenciaron la presencia de Campylobacter fetus y los resultados moleculares identificaron la subespecie venerealis. El resultado de la secuenciación reveló un e-value de 3e-33 con un valor de identidad del 99%, confirmando la subespecie venerealis // The aim of this study was to report the diagnosis of Campylobacter fetus venerealis (Cfv) in a bovine fetus by a multiplex Polimerase chain reaction test (PCR). The sample was submitted to the following diagnostic tests: bacteriological culture and direct immunofluorescence test (DIFT) and specific molecular markers were used for identification of a 142bp segment associated to the Cfv parA plasmid gene. This segment was sequenced and analyzed with BlastN. Results from the bacteriological tests and DIFT demonstrated Campylobacter fetus presence, while the molecular methods confirmed Cfv. Sequencing results revealed a 3e-33 e-... Presentar Todo |
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Palabras claves : |
ABORTION; ABORTO; BOVINE; BOVINOS; CAMPYLOBACTER FETUS VENEREALIS; PCR; REACCIÓN EN CADENA DE LA POLIMERASA (PCR) MÚLTIPLE. |
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Thesagro : |
CAMPYLOBACTER. |
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Asunto categoría : |
L73 Enfermedades de los animales |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/3087/1/CDocuments-and-SettingsachiacchioMis-documentosA-BIBLIOTECA-INIA-TACUAREMBO-TODOARTICULOS-TECNICOS-INIA-EN-REVISTAS-ARBITRADASINIA-TACUAREMBOCARNE-Y-LANAVeterinaria-Montevideo2013v49n192p20-28.pdf
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Marc : |
LEADER 02600naa a2200337 a 4500
001 1050145
005 2019-09-30
008 2013 bl uuuu u00u1 u #d
100 1 $aBOVE, R.
245 $aDiagnóstico Campylobacter fetus venerealis por PCR, en un aborto bovino espontáneo // Diagnosis of Campylobacter fetus venerealis in aborted bovine fetus.
260 $c2013
500 $aHistory article: Recibido: 25/02/2013; Aprobado: 01/04/2013.
520 $aEn este trabajo se reporta el diagnóstico de Campylobacter fetus venerealis (Cfv) de un aborto bovino espontáneo utilizando la técnica de reacción en cadena de la polimerasa (PCR) múltiple. La muestra de fluido estomacal fetal fue sometida a otros métodos de diagnóstico para Campylobacter fetus: cultivo bacteriano e inmunoflurescencia directa (IFD). Adicionalmente se emplearon marcadores moleculares específicos de CFv que permitieron identificar una banda de 142pb asociada al gen plasmídico parA Cfv. Dicho amplicón fue secuenciado y analizado por blastN. Los resultados de los cultivos bacteriológicos e IFD evidenciaron la presencia de Campylobacter fetus y los resultados moleculares identificaron la subespecie venerealis. El resultado de la secuenciación reveló un e-value de 3e-33 con un valor de identidad del 99%, confirmando la subespecie venerealis // The aim of this study was to report the diagnosis of Campylobacter fetus venerealis (Cfv) in a bovine fetus by a multiplex Polimerase chain reaction test (PCR). The sample was submitted to the following diagnostic tests: bacteriological culture and direct immunofluorescence test (DIFT) and specific molecular markers were used for identification of a 142bp segment associated to the Cfv parA plasmid gene. This segment was sequenced and analyzed with BlastN. Results from the bacteriological tests and DIFT demonstrated Campylobacter fetus presence, while the molecular methods confirmed Cfv. Sequencing results revealed a 3e-33 e-value with a 99% identity, confirming subspecies venerealis.
650 $aCAMPYLOBACTER
653 $aABORTION
653 $aABORTO
653 $aBOVINE
653 $aBOVINOS
653 $aCAMPYLOBACTER FETUS VENEREALIS
653 $aPCR
653 $aREACCIÓN EN CADENA DE LA POLIMERASA (PCR) MÚLTIPLE
700 1 $aLÓPEZ, F.
700 1 $aPERERA, C.
700 1 $aCARRACELAS, B.
700 1 $aTORRES, D.
700 1 $aDE SOUZA, G.
700 1 $aAZAMBUJA, C.
700 1 $aBERMÚDEZ, J.
700 1 $aALZUGARAY, F.
700 1 $aMEDEROS, A.
773 $tVeterinaria (Montevideo), 2013$gv. 49, no. 192, p. 20-28.
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