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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
03/12/2019 |
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Actualizado : |
01/06/2020 |
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Tipo de producción científica : |
Capítulo en Libro Técnico-Científico |
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Autor : |
LADO, J.; ZACARÍAS, J.; RODRIGO, M.J.; ZACARÍAS, L. |
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Afiliación : |
JOANNA LADO LINDNER, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JAIME ZACARIAS, Instituto de Agroquímica y Tecnología de Alimentos, Consejo Superior de Investigaciones Científicas (IATA-CSIC), Spain; MARÍA JESÚS RODRIGO, Instituto de Agroquímica y Tecnología de Alimentos, Consejo Superior de Investigaciones Científicas (IATA-CSIC), Spain; LORENZO ZACARÍAS, Instituto de Agroquímica y Tecnología de Alimentos, Consejo Superior de Investigaciones Científicas (IATA-CSIC), Spain. |
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Título : |
Visualization of carotenoid-storage structures in fruits by transmission electron microscopy. |
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Fecha de publicación : |
2020 |
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Fuente / Imprenta : |
In: Rodríguez-Concepción M., Welsch R. (eds). Plant and Food Carotenoids. Methods in Molecular Biology, 2020, vol 2083. Pages 235-244. DOI: https://doi.org/10.1007/978-1-4939-9952-1_18 |
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ISBN : |
978-1-4939-9951-4 (print) / 978-1-4939-9952-1 (e-book) |
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ISSN : |
1064-3745 (print) / 1940-6029 (electronic) |
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DOI : |
10.1007/978-1-4939-9952-1_18 |
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Idioma : |
Inglés |
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Notas : |
Article history: First Online: 20 November 2019. |
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Contenido : |
ABSTRACT.
Plastids are cell organelles that, beside other functions, have the capability to store carotenoids in specialized structures, which may vary among the different plant species, tissues or according to the carotenoid complement. Fruits are an important source of carotenoids, and during ripening, chloroplasts differentiate into chromoplasts that are able to accumulate large amounts of carotenoids, rendering then the characteristic fruit coloration. Whereas lycopene or β-carotene may accumulate as crystal in the chromoplasts of some fruit, other xanthophyll-accumulating fruits differentiate plastoglobuli as a preferred system to enhance carotenoids stability and storage. Visualization of plastid ultrastructure and their transformation during ripening or in fruit of contrasting coloration are fundamental objectives within carotenoids research in fruits. Therefore, in this chapter, we describe a protocol for the visualization and analysis of plastid ultrastructure by transmission electron microscopy (TEM), specially designed and adapted to fruit tissues.
© Springer Science+Business Media |
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Palabras claves : |
CAROTENOIDS; Chloroplast; Chromoplast; CITRUS FRUITS; Plastids; Plastoglobuli; Transmission electron microscopy. |
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Thesagro : |
CITRUS. |
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Asunto categoría : |
-- |
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Marc : |
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001 1060494
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022 $a1064-3745 (print) / 1940-6029 (electronic)
024 7 $a10.1007/978-1-4939-9952-1_18$2DOI
100 1 $aLADO, J.
245 $aVisualization of carotenoid-storage structures in fruits by transmission electron microscopy.$h[electronic resource]
260 $c2020
500 $aArticle history: First Online: 20 November 2019.
520 $aABSTRACT. Plastids are cell organelles that, beside other functions, have the capability to store carotenoids in specialized structures, which may vary among the different plant species, tissues or according to the carotenoid complement. Fruits are an important source of carotenoids, and during ripening, chloroplasts differentiate into chromoplasts that are able to accumulate large amounts of carotenoids, rendering then the characteristic fruit coloration. Whereas lycopene or β-carotene may accumulate as crystal in the chromoplasts of some fruit, other xanthophyll-accumulating fruits differentiate plastoglobuli as a preferred system to enhance carotenoids stability and storage. Visualization of plastid ultrastructure and their transformation during ripening or in fruit of contrasting coloration are fundamental objectives within carotenoids research in fruits. Therefore, in this chapter, we describe a protocol for the visualization and analysis of plastid ultrastructure by transmission electron microscopy (TEM), specially designed and adapted to fruit tissues. © Springer Science+Business Media
650 $aCITRUS
653 $aCAROTENOIDS
653 $aChloroplast
653 $aChromoplast
653 $aCITRUS FRUITS
653 $aPlastids
653 $aPlastoglobuli
653 $aTransmission electron microscopy
700 1 $aZACARÍAS, J.
700 1 $aRODRIGO, M.J.
700 1 $aZACARÍAS, L.
773 $tIn: Rodríguez-Concepción M., Welsch R. (eds). Plant and Food Carotenoids. Methods in Molecular Biology, 2020, vol 2083. Pages 235-244. DOI: https://doi.org/10.1007/978-1-4939-9952-1_18
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Registro original : |
INIA Las Brujas (LB) |
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