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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
01/10/2014 |
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Actualizado : |
09/10/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
ALQUEZAR, B.; RODRIGO, M.J.; LADO, J.; ZACARÍAS, L. |
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Afiliación : |
JOANNA LADO LINDNER, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. |
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Título : |
A comparative physiological and transcriptional study of carotenoid biosynthesis in white and red grapefruit (Citrus paradisi Macf.). |
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Fecha de publicación : |
2013 |
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Fuente / Imprenta : |
Tree Genetics & Genomes, 2013, v.9, no.5, p.1257-1269. |
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ISSN : |
1614-2942 |
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DOI : |
10.1007/s11295-013-0635-7 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: 30 January 2013 / Revised: 26 March 2013 / Accepted: 15 April 2013 / Published online: 15 May 2013. |
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Contenido : |
ABSTRACT.
Accumulation of lycopene in citrus fruits is an unusual feature restricted to selected mutants. Grapefruit (Citrus paradisi Macf.) is the Citrus specie with greater number of red-fleshed mutants, but the molecular bases of this alteration are not fully understood. To gain knowledge into the mechanisms implicated in this alteration, we conducted a comparative analysis of carotenoid profile and of the expression of genes related to carotenoid biosynthesis and catabolism in flavedo and pulp of two grapefruit cultivars with marked differences in colouration: the white Marsh and the red Star Ruby. Mature green fruit of Marsh accumulated chloroplastic carotenoids, while mature tissues lacked carotenoids. However, accumulation of downstream products such as abscisic acid (ABA) and expression of its biosynthetic genes, 9-cis-epoxycarotenoid dioxygenase (NCED1 and NCED2), increased after the onset of colouration. In contrast, red grapefruit accumulated lycopene, phytoene and phytofluene, while ABA content and NCED gene expression were lower than in Marsh, suggesting a blockage in the carotenoid biosynthetic pathway. Expression analysis of three genes of the isoprenoid pathway and nine of the carotenoid biosynthetic pathway revealed virtually no differences in flavedo and pulp between both genotypes, except for the chromoplast-specific lycopene cyclase 2 (β-LCY2) which was lower in the pulp of the red grapefruit. The proportion in the expression of the allele with high (β-LCY2a) and low (β-LCY2b) activity was also similar in the pulp of both genotypes. Therefore, results suggest that reduced expression of β-LCY2 appears to be responsible of lycopene accumulation in the red Star Ruby grapefruit.
© 2013 Springer-Verlag Berlin Heidelberg. MenosABSTRACT.
Accumulation of lycopene in citrus fruits is an unusual feature restricted to selected mutants. Grapefruit (Citrus paradisi Macf.) is the Citrus specie with greater number of red-fleshed mutants, but the molecular bases of this alteration are not fully understood. To gain knowledge into the mechanisms implicated in this alteration, we conducted a comparative analysis of carotenoid profile and of the expression of genes related to carotenoid biosynthesis and catabolism in flavedo and pulp of two grapefruit cultivars with marked differences in colouration: the white Marsh and the red Star Ruby. Mature green fruit of Marsh accumulated chloroplastic carotenoids, while mature tissues lacked carotenoids. However, accumulation of downstream products such as abscisic acid (ABA) and expression of its biosynthetic genes, 9-cis-epoxycarotenoid dioxygenase (NCED1 and NCED2), increased after the onset of colouration. In contrast, red grapefruit accumulated lycopene, phytoene and phytofluene, while ABA content and NCED gene expression were lower than in Marsh, suggesting a blockage in the carotenoid biosynthetic pathway. Expression analysis of three genes of the isoprenoid pathway and nine of the carotenoid biosynthetic pathway revealed virtually no differences in flavedo and pulp between both genotypes, except for the chromoplast-specific lycopene cyclase 2 (β-LCY2) which... Presentar Todo |
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Palabras claves : |
ABA; CAROTENOIDS; GENE EXPRESSION; GRAPEFRUIT; LYCOPENE. |
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Thesagro : |
CAROTENO; CITRUS. |
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Asunto categoría : |
F01 Cultivo |
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Marc : |
LEADER 02838naa a2200277 a 4500
001 1050762
005 2019-10-09
008 2013 bl uuuu u00u1 u #d
022 $a1614-2942
024 7 $a10.1007/s11295-013-0635-7$2DOI
100 1 $aALQUEZAR, B.
245 $aA comparative physiological and transcriptional study of carotenoid biosynthesis in white and red grapefruit (Citrus paradisi Macf.).$h[electronic resource]
260 $c2013
500 $aArticle history: Received: 30 January 2013 / Revised: 26 March 2013 / Accepted: 15 April 2013 / Published online: 15 May 2013.
520 $aABSTRACT. Accumulation of lycopene in citrus fruits is an unusual feature restricted to selected mutants. Grapefruit (Citrus paradisi Macf.) is the Citrus specie with greater number of red-fleshed mutants, but the molecular bases of this alteration are not fully understood. To gain knowledge into the mechanisms implicated in this alteration, we conducted a comparative analysis of carotenoid profile and of the expression of genes related to carotenoid biosynthesis and catabolism in flavedo and pulp of two grapefruit cultivars with marked differences in colouration: the white Marsh and the red Star Ruby. Mature green fruit of Marsh accumulated chloroplastic carotenoids, while mature tissues lacked carotenoids. However, accumulation of downstream products such as abscisic acid (ABA) and expression of its biosynthetic genes, 9-cis-epoxycarotenoid dioxygenase (NCED1 and NCED2), increased after the onset of colouration. In contrast, red grapefruit accumulated lycopene, phytoene and phytofluene, while ABA content and NCED gene expression were lower than in Marsh, suggesting a blockage in the carotenoid biosynthetic pathway. Expression analysis of three genes of the isoprenoid pathway and nine of the carotenoid biosynthetic pathway revealed virtually no differences in flavedo and pulp between both genotypes, except for the chromoplast-specific lycopene cyclase 2 (β-LCY2) which was lower in the pulp of the red grapefruit. The proportion in the expression of the allele with high (β-LCY2a) and low (β-LCY2b) activity was also similar in the pulp of both genotypes. Therefore, results suggest that reduced expression of β-LCY2 appears to be responsible of lycopene accumulation in the red Star Ruby grapefruit. © 2013 Springer-Verlag Berlin Heidelberg.
650 $aCAROTENO
650 $aCITRUS
653 $aABA
653 $aCAROTENOIDS
653 $aGENE EXPRESSION
653 $aGRAPEFRUIT
653 $aLYCOPENE
700 1 $aRODRIGO, M.J.
700 1 $aLADO, J.
700 1 $aZACARÍAS, L.
773 $tTree Genetics & Genomes, 2013$gv.9, no.5, p.1257-1269.
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| 221. |  | LADO, J.; CUELLAR, F.; RODRIGO, M.J.; ZACARÍAS, L. Nutritional composition of mandarins. In: Simmonds, M.S.J., Preedy, V.R. (Eds.), 2016, Nutritional Composition of Fruit Cultivars. Academic Press, 419-443.(Chap. 18).| Tipo: Capítulo en Libro Técnico-Científico |
| Biblioteca(s): INIA Las Brujas. |
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| 223. | | PECHI MIDÓN, E; BUENAHORA, J. P32. Monitoreo de Diaphorina citri Kuwayama (Hemiptera: Psyllidae) y entomofauna benéfica en cítricos del sur de Uruguay. [Poster]. Posters. In: Sociedad Uruguaya de Fitopatología (SUFIT). Jornada Uruguaya de Fitopatología, 7., Jornada Uruguaya de Protección Vegetal, 5., 10 noviembre 2023, Montevideo, Uruguay. Libro de resúmenes. 30 años SUFIT, 1993-2023. Montevideo (UY): Sociedad Uruguay de Fitopatología (SUFIT), 2023. p. 58. Financiamiento: Procisur; Proyecto: Desarrollo y promoción de herramientas innovadoras para la prevención y mitigación del efecto de HLB en los países miembros del PROCISUR. -- Autores correspondencia: e-mail: jbuenahora@inia.org.uy ;...| Tipo: Abstracts/Resúmenes |
| Biblioteca(s): INIA Las Brujas. |
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| 225. | | ASPLANATO, G. Reconocimiento, daños e importancia económica. (Capítulo II) In: ASPLANATO, G. (Ed.). El minador de la hoja de los cítricos, Phyllocnistis citrella (Lepidotera: gracillariidae): Bioecología y control biológico. Montevideo (Uruguay): INIA, 2009. p. 13-22 (Serie FPTA-INIA ; 24)| Tipo: Capítulo en Libro Técnico-Científico |
| Biblioteca(s): INIA Las Brujas; INIA Tacuarembó. |
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| 229. | | PEYROU, M.; RIGAMONTI, N.; DEL CAMPO, R.; RUSSI, P.; LARRECHART, L.; PEREZ, E.; MARA, H. Técnicas biológicas, serológicas y moleculares para la detección asintomática de Xanthomonas axonopodis pv. citri Agrociencia Uruguay, 2012, v.16, no.1, p.124-133| Tipo: Artículos en Revistas Indexadas Nacionales | Circulación / Nivel : B - 2 |
| Biblioteca(s): INIA La Estanzuela; INIA Las Brujas. |
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| 232. | | SALVO, M.; REY, F.; ARRUABARRENA, A.; GAMBETTA, G.; RODRIGO, M. J.; ZACARÍAS, L.; LADO, J. Transcriptional analysis of C-repeat binding factors in fruit of citrus species with differential sensitivity to chilling injury during postharvest storage. International Journal of Molecular Sciences, 2 January 2021, Volume 22, Issue 2, Article number 804, Pages 1-20. OPEN ACCESS. Doi: https://doi.org/10.3390/ijms22020804 Article history: Received 9 December 2020; Revised 5 January 2021; Accepted 12 January 2021; Published 15 January 2021.
This work was supported by a research grant from the National Agency of Research and Innovation (ANII-Uruguay) by the...| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA Las Brujas. |
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| 235. | | PAGLIANO, G.; UMANA, R.; PRITSCH, C.; RIVAS, F.; DURÁN-VILA, N. Occurrence, prevalence and distribution of Citrus viroids in Uruguay. [Short communication]. Journal of Plant Pathology, 2013, v.95, no.3, p.631-635.| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : A - 2 |
| Biblioteca(s): INIA Las Brujas. |
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| 236. | | LADO, J.; MOLTINI, A.; PINTOS, P.; LUQUE, E.; GONCALVEZ, L.; RIVAS, F.; ALCAIRE, F.; ARES, G. Unraveling factors affecting consumers' liking of novel Uruguayan mandarins. [Descifrando los factores que afectan la aceptabilidad de nuevas mandarinas Uruguayas]. [Descifrando os fatores que afetam a aceitabilidade de novas tangerinas uruguaias]. Agrociencia Uruguay, 2021, vol. 25, n.2, article E540. DOI: https://doi.org/10.31285/AGRO.25.540 Article history: Received 10 Feb 2021; Accepted 3 Aug 2021; Published 18 Aug 2021.
Editor: Gustavo González-Neves, Universidad de la República, Montevideo, Uruguay.
Correspondence: Joanna Lado, mail: jlado@inia.org.uy| Tipo: Artículos en Revistas Indexadas Nacionales | Circulación / Nivel : Nacional - -- |
| Biblioteca(s): INIA Las Brujas. |
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| 237. | | LADO, J.; RIVAS, F.; MOLTINI, A.; ALCAIRE, F.; ARES, G. Uruguayan consumers' perception of mandarins: insights for selection and marketing of newcultivars. [Percepción de las mandarinas por los consumidores uruguayos: aportes para la selección y la promoción denuevos cultivares. ]. [Percepção dos consumidores uruguaios sobre tangerinas: contribuições para a seleção e comercialização de novas cultivares.] Section: Food Technology. Agrociencia Uruguay, 2021, vol. 25, n.2, article e530. Doi: https://doi.org/10.31285/AGRO.25.530 Article history: Received 02 Feb 2021; Accepted 09 Apr 2021, Published03 Jun 2021.
Editor: Gustavo González-Neves, Universidad de la República, Montevideo, Uruguay.
Correspondence: Joanna Lado, mail: jlado@inia.org.uy| Tipo: Artículos en Revistas Indexadas Nacionales | Circulación / Nivel : Nacional - -- |
| Biblioteca(s): INIA Las Brujas. |
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| Registros recuperados : 370 | |
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