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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
29/09/2014 |
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Actualizado : |
24/06/2021 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
FEDERICI, M.; MARCONDES, J.A.; PICCHI, S.C.; STUCHI, E.S.; FADEL, A.L.; LAIA, M.L.; LEMOS, M.V.F. |
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Afiliación : |
MARIA TERESA FEDERICI RODRIGUEZ, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. |
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Título : |
Xylella fastidiosa: An in vivo system to study possible survival strategies within citrus xylem vessels based on global gene expression analysis. |
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Fecha de publicación : |
2012 |
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Fuente / Imprenta : |
Electronic Journal of Biotechnology, 2012, v.15, no.3, p.1-33. |
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ISSN : |
0717-3458 |
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DOI : |
http://dx.doi.org/10.2225/vol15-issue3-fulltext-4 |
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Idioma : |
Inglés |
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Contenido : |
ABSTRACT.
Xylella fastidiosa inhabits the plant xylem, a nutrient-poor environment, so that mechanisms to sense and respond to adverse environmental conditions are extremely important for bacterial survival in the plant host. Although the complete genome sequences of different Xylella strains have been determined, little is known about stress responses and gene regulation in these organisms. In this work, a DNA microarray was constructed containing 2,600 ORFs identified in the genome sequencing project of Xylella fastidiosa 9a5c strain, and used to check global gene expression differences in the bacteria when it is infecting a symptomatic and a tolerant citrus tree. Different patterns of expression were found in each variety, suggesting that bacteria are responding differentially according to each plant xylem environment. The global gene expression profile was determined and several genes related to bacterial survival in stressed conditions were found to be differentially expressed between varieties, suggesting the involvement of different strategies for adaptation to the environment. The expression pattern of some genes related to the heat shock response, toxin and detoxification processes, adaptation to atypical conditions, repair systems as well as some regulatory genes are discussed in this paper. DNA microarray proved to be a powerful technique for global transcriptome analyses. This is one of the first studies of Xylella fastidiosa gene expression in vivo which helped to increase insight into stress responses and possible bacterial survival mechanisms in the nutrient-poor environment of xylem vessels. MenosABSTRACT.
Xylella fastidiosa inhabits the plant xylem, a nutrient-poor environment, so that mechanisms to sense and respond to adverse environmental conditions are extremely important for bacterial survival in the plant host. Although the complete genome sequences of different Xylella strains have been determined, little is known about stress responses and gene regulation in these organisms. In this work, a DNA microarray was constructed containing 2,600 ORFs identified in the genome sequencing project of Xylella fastidiosa 9a5c strain, and used to check global gene expression differences in the bacteria when it is infecting a symptomatic and a tolerant citrus tree. Different patterns of expression were found in each variety, suggesting that bacteria are responding differentially according to each plant xylem environment. The global gene expression profile was determined and several genes related to bacterial survival in stressed conditions were found to be differentially expressed between varieties, suggesting the involvement of different strategies for adaptation to the environment. The expression pattern of some genes related to the heat shock response, toxin and detoxification processes, adaptation to atypical conditions, repair systems as well as some regulatory genes are discussed in this paper. DNA microarray proved to be a powerful technique for global transcriptome analyses. This is one of the first studies of Xylella fastidiosa gene expression in vivo which helped... Presentar Todo |
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Thesagro : |
CITRUS; CYDIA. |
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Asunto categoría : |
F01 Cultivo |
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Marc : |
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024 7 $ahttp://dx.doi.org/10.2225/vol15-issue3-fulltext-4$2DOI
100 1 $aFEDERICI, M.
245 $aXylella fastidiosa$bAn in vivo system to study possible survival strategies within citrus xylem vessels based on global gene expression analysis.$h[electronic resource]
260 $c2012
520 $aABSTRACT. Xylella fastidiosa inhabits the plant xylem, a nutrient-poor environment, so that mechanisms to sense and respond to adverse environmental conditions are extremely important for bacterial survival in the plant host. Although the complete genome sequences of different Xylella strains have been determined, little is known about stress responses and gene regulation in these organisms. In this work, a DNA microarray was constructed containing 2,600 ORFs identified in the genome sequencing project of Xylella fastidiosa 9a5c strain, and used to check global gene expression differences in the bacteria when it is infecting a symptomatic and a tolerant citrus tree. Different patterns of expression were found in each variety, suggesting that bacteria are responding differentially according to each plant xylem environment. The global gene expression profile was determined and several genes related to bacterial survival in stressed conditions were found to be differentially expressed between varieties, suggesting the involvement of different strategies for adaptation to the environment. The expression pattern of some genes related to the heat shock response, toxin and detoxification processes, adaptation to atypical conditions, repair systems as well as some regulatory genes are discussed in this paper. DNA microarray proved to be a powerful technique for global transcriptome analyses. This is one of the first studies of Xylella fastidiosa gene expression in vivo which helped to increase insight into stress responses and possible bacterial survival mechanisms in the nutrient-poor environment of xylem vessels.
650 $aCITRUS
650 $aCYDIA
700 1 $aMARCONDES, J.A.
700 1 $aPICCHI, S.C.
700 1 $aSTUCHI, E.S.
700 1 $aFADEL, A.L.
700 1 $aLAIA, M.L.
700 1 $aLEMOS, M.V.F.
773 $tElectronic Journal of Biotechnology, 2012$gv.15, no.3, p.1-33.
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INIA Las Brujas (LB) |
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| Registros recuperados : 239 | |
| 1. |  | PEREYRA GODAY, F.; JEBARI, A.; TAKAHASHI, T.; ROVIRA, P.J.; AYALA, W.; LEE, M.R.F.; RIVERO, M.J.; MCAULIFFE, G.A. Carbon footprint of mixed farming crop-livestock rotational-based grazing beef systems using long term experimental data. Research article. Agronomy for Sustainable Development, 2024, Volume 44, Issue 4, article 41. https://doi.org/10.1007/s13593-024-00977-1 -- OPEN ACCESS. Article history: Accepted 01 July 2024; Published 15 July 2024. -- Corresponding author: Pereyra-Goday, F.; Instituto Nacional de Investigación Agropecuaria (INIA), Treinta y Tres, Uruguay; email:fpereyragoday@gmail.com -- Funding: This...| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA Las Brujas. |
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| 3. | | CAZZULI, F.; BREMM, C.; JAURENA, M.; POPPI, D.; DURANTE, M.; BENVENUTTI, M.A.; SAVIAN, J.V.; DEVINCENZI, T.; ROVIRA, P.J.; LAGOMARSINO, X.; HIRIGOYEN, A.; LATTANZI, F. The defoliation dynamics of a stockpiled native grassland pasture follow similar patterns between supplemented and unsupplemented beef calves. Grass and Forage Science, 2023, Vol.78, Issue 2, pages 306-316. doi: https://doi.org/10.1111/gfs.12608 Article history: Received 24 November 2022; Revised 14 March 2023; Accepted 15 March 2023. -- Correspondence author: Cazzuli, F.; Instituto Nacional de Investigación Agropecuaria (INIA), Programa Pasturas y Forrajes, Estación...| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA Las Brujas. |
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| 4. | | CAZZULI, F.; DURANTE, M.; HIRIGOYEN, A.; SÁNCHEZ, J.; ROVIRA, P.J.; BERETTA, V.; SIMEONE, A.; JAURENA, M.; SAVIAN, J.V.; POPPI, D.; MONTOSSI, F.; LAGOMARSINO, X.; LUZARDO, S.; BRITO, G.; VELAZCO, J.I.; LATTANZI, F.; BREMM, C. Dinámica de la respuesta a la suplementación invernal de bovinos en crecimiento sobre campo natural. Producción animal. Revista INIA Uruguay, Diciembre 2023, no.75 p.12-16. (Revista INIA; 75). *Grados.día: es una forma de estimar la temperatura acumulada, como la sumatoria de la diferencia entre la temperatura promedio de cada día y una
temperatura llamada "base", que en el caso de este estudio fue 0 °C. El día inicial de la...| Tipo: Artículos en Revistas Agropecuarias |
| Biblioteca(s): INIA Las Brujas. |
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| 5. | | CAZZULI, F.; DURANTE, M.; HIRIGOYEN, A.; SÁNCHEZ, J.; ROVIRA, P.J.; BERETTA, V.; SIMEONE, A.; JAURENA, M.; SAVIAN, J.V.; POPPI, D.; MONTOSSI, F.; LAGOMARSINO, X.; LUZARDO, S.; BRITO, G.; VELAZCO, J.I.; LATTANZI, F.; BREMM, C. Beef cattle grazing native grasslands may follow three different supplement response patterns. Grasses. 2023, Volume 2, Issue 3, pages 168-184. https://doi.org/10.3390/grasses2030014 --- OPEN ACCESS. Article history: Received 3 May 2023; Revised 1 July 2023; Accepted 13 July 2023; Published 7 August 2023. -- Academic Editor: Fabio Gresta. -- FUNDING: This research was funded by INIA Uruguay. -- LICENSE: This article is an open access...| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA Las Brujas. |
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| 6. | | LUZARDO, S.; SAADOUN, A.; CABRERA, M. C.; TEREVINTO, A.; BRUGNINI, G.; RODRIGUEZ, J.; DE SOUZA, G.; ROVIRA, P.J.; RUFO, C. Effect of beef long-storage under different temperatures and vacuum-packaging conditions on meat quality, oxidation processes and microbial growth. Journal of the Science of Food and Agriculture, 2023. https://doi.org/10.1002/jsfa.12999 -- OPEN ACCESS. Article history: Manuscript received 04 June 2023, Manuscript revised 09 August 2023, Manuscript accepted 19 September 2023, Accepted manuscript online 22 September 2023, Version of Record online 06 October 2023. -- Correspondence...| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA Las Brujas. |
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| 7. | | FEDERICI, M.; RIGAMONTI, N.; ROVIRA, P.J.; TORRES, P.; FERRARI, G.; SIMÓN, C.; GARCIA, C.; REGGIO, A.; CIGANDA, V. Diversidad de cianobacterias en cuerpos de agua de uso agropecuario: desafíos, oportunidades y abordaje molecular. Sustentabilidad. Revista INIA Uruguay, Setiembre 2023, no.74 p.62-66. (Revista INIA; 74).| Tipo: Artículos en Revistas Agropecuarias |
| Biblioteca(s): INIA Las Brujas. |
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| 9. | | ROVIRA, P.J.; BRUGNINI , G.; RODRIGUEZ, J.; CABRERA, M.C.; SAADOUN, A.; DE SOUZA, G.; LUZARDO, S.; RUFO, C. Microbiological changes during long-storage of beef meat under different temperature and vacuum-packaging conditions. Foods, 2023, volume 12, issue 4, article 694. OPEN ACCESS. doi: https://doi.org/10.3390/foods12040694 Article history: Received 28 December 2022; Revised 26 January 2023; Accepted 30 January 2023; Published 6 February 2023. -- Correspondence: Santiago Luzardo, mail: sluzardo@inia.org.uy (S.L.); Caterina Rufo, mail: crufo@fq.edu.uy (C.R.)...| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA Las Brujas. |
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| 10. | | CAZZULI, F.; SÁNCHEZ, J.; HIRIGOYEN, A.; ROVIRA, P.J.; BERETTA, V.; SIMEONE, A.; JAURENA, M.; DURANTE, M.; SAVIAN, J.V.; POPPI, D.; MONTOSSI, F.; LAGOMARSINO, X.; LUZARDO, S.; BRITO, G.; VELAZCO, J.I.; BREMM, C.; LATTANZI, F. Supplement feed efficiency of growing beef cattle grazing native Campos grasslands during winter: a collated analysis. Translational Animal Science. 2023, Volume 7, Issue 1, txad028. https://doi.org/10.1093/tas/txad028 -- OPEN ACCESS Article history: Received 03 October 2022; Accepted 09 March 2023; Published 10 March 2023; Corrected and typeset 01 April 2023. -- Corresponding author: fcazzuli@inia.org.uy -- Issue Section: Forage Based Livestock Systems. -- License:...| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA Las Brujas. |
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| 13. | | FEDERICI, M.; RIGAMONTI, N.; FERRARI, G.; ROVIRA, P.J.; TORRES, P.; GUERRA, S.; SIMÓN, C.; FERNÁNDEZ-CIGANDA, S. Análisis de comunidades de cianobacterias y microorganismos asociados al sedimento mediante secuenciación del gen 16S ARNr y qPCR en el embalse rincón del bonete. 161. (resúmen) Áreas temáticas: Ciencias del Mar y Limnología. In: Physiological Mini Reviews, 2022, volume 15, Special Issue: III (3er) Congreso Nacional de Biociencias Octubre 2022, Montevideo, Uruguay. p.145. Resumen publicado en las jornadas de BIOCIENCIAS: II Jornadas Binacionales Argentina-Uruguay; III Congreso Nacional 2022 "Ciencia para el desarrollo sustentable".| Tipo: Abstracts/Resúmenes |
| Biblioteca(s): INIA Las Brujas. |
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| 15. | | FEDERICI, M.; RIGAMONTI, N.; ROVIRA, P.J.; TORRES, P.; MARTÍNEZ, S.; FERRARI, G.; SIMÓN, C.; AUBRIOT, L.; CIGANDA, V. Aplicación de técnicas clásicas de microscopía y basadas en ADN para el análisis de las comunidades de microorganismos y cianobacterias del sedimento en el Embalse Rincón del Bonete - Uruguay. [resumen]. APLICACIÓN DE TÉCNICAS CLÁSICAS DE MICROSCOPÍA Y
BASADAS EN ADN PARA EL ANÁLISIS DE LAS COMUNIDADES DE MICROORGANISMOS Y CIANOBACTERIAS DEL SEDIMENTO EN EL EMBALSE RINCÓN DEL BONETE- URUGUAY In: REDBIO México 2022, XI Congreso, "Biotecnología productiva y sostenible". Libro de resúmenes. 12-14 octubre 2022, Yucatán, México. p.127. Agradecimientos: A los Institutos INIA y LATU con sus respectivas autoridades por la cofinanciación del Proyecto: "Desarrollo y Aplicación de nuevas herramientas moleculares y espectrales para el estudio de las comunidades de...| Tipo: Abstracts/Resúmenes |
| Biblioteca(s): INIA Las Brujas. |
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| 16. | | CAZZULI, F.; SÁNCHEZ, J.; HIRIGOYEN, A.; ROVIRA, P.J.; BERETTA, V.; SIMEONE, A.; JAURENA, M.; DURANTE, M.; SAVIAN, J.V.; POPPI, D.; MONTOSSI, F.; LAGOMARSINO, X.; LUZARDO, S.; BRITO, G.; VELAZCO, J.I.; BREMM, C.; LATTANZI, F. Eficiencia de uso de suplementos energético-proteicos en vacunos en invierno sobre campo natural: análisis de casi 30 años de ensayos nacionales. Producción Animal. Revista INIA Uruguay, Marzo 2022, no.68, p.19-22. (Revista INIA; 68).| Tipo: Artículos en Revistas Agropecuarias |
| Biblioteca(s): INIA Las Brujas. |
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| 18. | | FEDERICI, M.; VALLEJOS, M.; SIMÓN, C.; ROVIRA, P.J.; CIGANDA, V.; RIGAMONTI, N.; FERRARI, G. Impacto de las cianotoxinas en la producción agropecuaria y el ambiente. Sustentabilidad. Revista INIA Uruguay, Marzo 2022, no.68, p.79-83 (Revista INIA; 68).| Tipo: Artículos en Revistas Agropecuarias |
| Biblioteca(s): INIA Las Brujas. |
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| 19. | | AYALA, W.; BENDERSKY, D.; PARUELO, J.; ROVIRA, P.J. The importance of Campos ecosystems as a world food producer and as a provider of ecosystem services. In: MUREITHI, J.G.; NYANGITO, M.M.; WAMUONGO, J.W.; NJOKA, J.; NYAMBATI; E.M.; MIANO,D.; MBUKU, S.; OKOTI, M.; MARITIM. F., ed. Sustainable use of grassland and rangeland resources for improved livelihoods. Proceedings. Nairobi, (Kenia): KALRO, 2022. p. 655-660| Tipo: Trabajos en Congresos/Conferencias |
| Biblioteca(s): INIA Treinta y Tres. |
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| Registros recuperados : 239 | |
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