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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
29/09/2014 |
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Actualizado : |
24/06/2021 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
FEDERICI, M.; MARCONDES, J.A.; PICCHI, S.C.; STUCHI, E.S.; FADEL, A.L.; LAIA, M.L.; LEMOS, M.V.F. |
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Afiliación : |
MARIA TERESA FEDERICI RODRIGUEZ, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. |
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Título : |
Xylella fastidiosa: An in vivo system to study possible survival strategies within citrus xylem vessels based on global gene expression analysis. |
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Fecha de publicación : |
2012 |
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Fuente / Imprenta : |
Electronic Journal of Biotechnology, 2012, v.15, no.3, p.1-33. |
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ISSN : |
0717-3458 |
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DOI : |
http://dx.doi.org/10.2225/vol15-issue3-fulltext-4 |
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Idioma : |
Inglés |
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Contenido : |
ABSTRACT.
Xylella fastidiosa inhabits the plant xylem, a nutrient-poor environment, so that mechanisms to sense and respond to adverse environmental conditions are extremely important for bacterial survival in the plant host. Although the complete genome sequences of different Xylella strains have been determined, little is known about stress responses and gene regulation in these organisms. In this work, a DNA microarray was constructed containing 2,600 ORFs identified in the genome sequencing project of Xylella fastidiosa 9a5c strain, and used to check global gene expression differences in the bacteria when it is infecting a symptomatic and a tolerant citrus tree. Different patterns of expression were found in each variety, suggesting that bacteria are responding differentially according to each plant xylem environment. The global gene expression profile was determined and several genes related to bacterial survival in stressed conditions were found to be differentially expressed between varieties, suggesting the involvement of different strategies for adaptation to the environment. The expression pattern of some genes related to the heat shock response, toxin and detoxification processes, adaptation to atypical conditions, repair systems as well as some regulatory genes are discussed in this paper. DNA microarray proved to be a powerful technique for global transcriptome analyses. This is one of the first studies of Xylella fastidiosa gene expression in vivo which helped to increase insight into stress responses and possible bacterial survival mechanisms in the nutrient-poor environment of xylem vessels. MenosABSTRACT.
Xylella fastidiosa inhabits the plant xylem, a nutrient-poor environment, so that mechanisms to sense and respond to adverse environmental conditions are extremely important for bacterial survival in the plant host. Although the complete genome sequences of different Xylella strains have been determined, little is known about stress responses and gene regulation in these organisms. In this work, a DNA microarray was constructed containing 2,600 ORFs identified in the genome sequencing project of Xylella fastidiosa 9a5c strain, and used to check global gene expression differences in the bacteria when it is infecting a symptomatic and a tolerant citrus tree. Different patterns of expression were found in each variety, suggesting that bacteria are responding differentially according to each plant xylem environment. The global gene expression profile was determined and several genes related to bacterial survival in stressed conditions were found to be differentially expressed between varieties, suggesting the involvement of different strategies for adaptation to the environment. The expression pattern of some genes related to the heat shock response, toxin and detoxification processes, adaptation to atypical conditions, repair systems as well as some regulatory genes are discussed in this paper. DNA microarray proved to be a powerful technique for global transcriptome analyses. This is one of the first studies of Xylella fastidiosa gene expression in vivo which helped... Presentar Todo |
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Thesagro : |
CITRUS; CYDIA. |
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Asunto categoría : |
F01 Cultivo |
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Marc : |
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024 7 $ahttp://dx.doi.org/10.2225/vol15-issue3-fulltext-4$2DOI
100 1 $aFEDERICI, M.
245 $aXylella fastidiosa$bAn in vivo system to study possible survival strategies within citrus xylem vessels based on global gene expression analysis.$h[electronic resource]
260 $c2012
520 $aABSTRACT. Xylella fastidiosa inhabits the plant xylem, a nutrient-poor environment, so that mechanisms to sense and respond to adverse environmental conditions are extremely important for bacterial survival in the plant host. Although the complete genome sequences of different Xylella strains have been determined, little is known about stress responses and gene regulation in these organisms. In this work, a DNA microarray was constructed containing 2,600 ORFs identified in the genome sequencing project of Xylella fastidiosa 9a5c strain, and used to check global gene expression differences in the bacteria when it is infecting a symptomatic and a tolerant citrus tree. Different patterns of expression were found in each variety, suggesting that bacteria are responding differentially according to each plant xylem environment. The global gene expression profile was determined and several genes related to bacterial survival in stressed conditions were found to be differentially expressed between varieties, suggesting the involvement of different strategies for adaptation to the environment. The expression pattern of some genes related to the heat shock response, toxin and detoxification processes, adaptation to atypical conditions, repair systems as well as some regulatory genes are discussed in this paper. DNA microarray proved to be a powerful technique for global transcriptome analyses. This is one of the first studies of Xylella fastidiosa gene expression in vivo which helped to increase insight into stress responses and possible bacterial survival mechanisms in the nutrient-poor environment of xylem vessels.
650 $aCITRUS
650 $aCYDIA
700 1 $aMARCONDES, J.A.
700 1 $aPICCHI, S.C.
700 1 $aSTUCHI, E.S.
700 1 $aFADEL, A.L.
700 1 $aLAIA, M.L.
700 1 $aLEMOS, M.V.F.
773 $tElectronic Journal of Biotechnology, 2012$gv.15, no.3, p.1-33.
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INIA Las Brujas (LB) |
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| Registros recuperados : 99 | |
| 1. |  | GONZÁLEZ SOSA, M.; SIERRA, C.A.; QUINCKE, A.; BAETHGEN, W.; TRUMBORE, S.; PRAVIA, V. High capacity of integrated crop-pasture systems to preserve old stable carbon evaluated in a 60-year-old experiment. Soil. 2024, Volume 10, Issue 2, pages 467-486. https://doi.org/10.5194/soil-10-467-2024 -- OPEN ACCESS. Article history: Received 8 November 2023, Discussion started 21 November 2023, Revised 25 April 2024, Accepted 1 May 2024, Published 5 July 2024. -- Correspondence: Maximiliano González-Sosa (mgonzalez@fagro.edu.uy) -- Published by...| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA Las Brujas. |
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| 2. | | BALDASSINI, P.; BAETHGEN, W.; CAMBA SANS, G.; QUINCKE, A.; PRAVIA, V.; TERRA, J.A.; MACEDO, F.; PIÑEIRO, G.; PARUELO, J. Carbon stocks and potential sequestration of Uruguayan soils. A road map to a comprehensive characterization of temporal and spatial changes to assess Carbon footprint. Original research. Frontiers in Sustainable Food Systems. 2023, Volume 7. https://doi.org/10.3389/fsufs.2023.1045734 Article history: Received 16 Sep 2022; Accepted 25 May 2023; Published 20 July 2023. -- Correspondence: Dr. Pablo Baldassini, Instituto Nacional de Investigación Agropecuaria, INIA La Estanzuela, Colonia, Uruguay. -- Edited by: Bruno José...| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA Las Brujas. |
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| 3. | | PARUELO, J.; LÓPEZ MARSICO, L.; BALDASSINI, P.; LEZAMA, F.; BAZZONI, B.; STAIANO, L.; NÚÑEZ, A.; GUIDO, A.; RÍOS, C.; TOMMASINO, A.; GALLEGO, F.; PEZZANI, F.; CAMBA SANS, G.; QUINCKE, A.; BAEZA, S.; PIÑEIRO, G.; BAETHGEN, W. Comment on: "Back to the future? Conservative grassland management can preserve soil health in the changing landscapes of Uruguay" On the risks of good intentions and poor evidence. EGUsphere [preprint], 2023. https://doi.org/10.5194/egusphere-2023-2023 -- OPEN ACCESS. Article history: Received 04 Sep 2023, Discussion started 14 Nov 2023. -- Correspondence to: José Paruelo (angie@email.com) -- License: This work is distributed under the Creative Commons Attribution 4.0 License...| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA Las Brujas. |
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| 4. | | BLUMETTO, O.; DE BARBIERI, I.; NAVAJAS, E.; BAETHGEN, W.; BECOÑA, G.; CIAPPESONI, G. Ganadería regenerativa en Uruguay. Montevideo (UY): INIA, 2023. 23 p. (INIA, Edición Especial). Revisión técnica: Ing. Agr. PhD Verónica Ciganda y DMV PhD Georgget Banchero. -- Esta publicación deriva del trabajo de investigación del Área de Recursos Naturales, Producción y Ambiente, en el marco del Sistema Ganadero Extensivo.| Biblioteca(s): INIA Las Brujas. |
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| 5. | | WILLEM, R.; NERVI, E.; BAETHGEN, W. Integrated catchment models for policy development and decision making. [Modelos integrados de cuencas hidrográficas para el desarrollo de políticas y la toma de decisiones.]. [Modelos integrados de baciaspara desenvolvimento e políticas e tomada de decisões.]. Advances in Water in Agroscience. Integrated catchment management. Agrociencia Uruguay, 2023, Vol.27(NE1), e1194. https://doi.org/10.31285/AGRO.27.1194 -- OPEN ACCESS. Article history: Received 15 May 2023; Accepted 14 August 2023; Published 06 February 2024. -- Editor: Ángela Gorgoglione, Universidad de la República, Montevideo, Uruguay. -- Correspondence: Willem Vervoort, willem.vervoort@sydney.edu.au...| Tipo: Artículos en Revistas Indexadas Nacionales | Circulación / Nivel : Nacional - -- |
| Biblioteca(s): INIA Las Brujas. |
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| 6. | | BLUMETTO, O.; DE BARBIERI, I.; NAVAJAS, E.; BAETHGEN, W.; VERA, B.; BECOÑA, G.; CIAPPESONI, G. Regenerative livestock farming in Uruguay. Montevideo (UY): INIA, 2023. 23 p. (INIA, Special Edition). Technical review: Ing. Agr. PhD Verónica Ciganda y DMV PhD Georgget Banchero. -- This publication derives from the research work of the Natural Resources, Production and Environment Area, within the framework of the Extensive Livestock...| Tipo: Ediciones Especiales |
| Biblioteca(s): INIA Las Brujas. |
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| 7. | | CARAM, N.; SOCA, P.; SOLLENBERGER, L.E.; BAETHGEN, W.; WALLAU, M.O.; MAILHOS, M.E. Studying beef production evolution to plan for ecological intensification of grazing ecosystems. Agricultural Systems, 2023, volume 205, Article number 103582. doi: https://doi.org/10.1016/j.agsy.2022.103582 Article history: Received 5 September 2022; Received in revised form 4 November 2022; Accepted 2 December 2022, available online 6 December 2022.
E-mail address: ncaramfernandezv@ufl.edu (N. Caram).| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA Treinta y Tres. |
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| 8. | | GELÓS, M.; NEIGHBUR, N.; KOK, P.; BADANO, L.; HASTINGS, F.; NERVI, E.; ALONSO, J.; NAVAS, R.; VERVOORT, W.; BAETHGEN, W. On the prediction of phosphorus fluxes in the santa lucía basin under different land use and management practices using swat model. [abstract] Theme 5 - Impact of phosphorus on environmental quality and on biodiversity. Oral presentation. In: Michelini, D.; Garaycochea, S. (Eds.). 7th Phosphorus in Soils and Plants Symposium (PSP7). "Towards a sustainable phosphorus utilization in agroecosystems." Book of abstracts. PSP7, 3-7 October 2022, Montevideo, Uruguay. p.81. Published By: The organizing committee of the 7th Symposium on Phosphorus in Soils and Plants (PSP7)- National Agricultural Research Institute and School of Agronomy, Universidad de la República, Uruguay.| Tipo: Abstracts/Resúmenes |
| Biblioteca(s): INIA Las Brujas. |
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| 20. | | COBON, D.H.; BAETHGEN, W.; LANDMAN, W; WILLIAMS, A.; VAN GARDEREN, E.A.; JOHNSTON, P.; MALHERBE, J.; MALULEKE, P.; KGAKATSI, I.B.; DAVIS, P. Agroclimatology in Grasslands. [Book chapter]. In: J. L. Hatfield, M. V.K. Sivakumar, J. H. Prueger, editors, Agroclimatology: Linking Agriculture to Climate, Agron. Monogr. 60. ASA, CSSA, and SSSA, Madison, WI. p. 1-55| Tipo: Capítulo en Libro Técnico-Científico |
| Biblioteca(s): INIA Las Brujas. |
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| Registros recuperados : 99 | |
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