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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha : |
20/07/2022 |
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Actualizado : |
20/07/2022 |
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Tipo de producción científica : |
Capítulo en Libro Técnico-Científico |
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Autor : |
CRISPO, M.; CHENOUARD, V.; DOS SANTOS-NETO P; TESSON, L.; SOUZA-NEVES, M.; HESLAN, J.M.; CUADRO, F.; ANEGÓN, I.; MENCHACA, A. |
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Afiliación : |
MARTINA CRISPO, Laboratory Animal Biotechnology Unit, Institut Pasteur de Montevideo, Montevideo, Uruguay.; VANESSA CHENOUARD, INSERM Centre de Recherche en Transplantation et Immunologie UMR 1064, Transgenesis Rat ImmunoPhenomic Facility (TRIP), Nantes, France.; PEDRO DOS SANTOS-NETO, Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay.; LAURENT TESSON, INSERM Centre de Recherche en Transplantation et Immunologie UMR 1064, Transgenesis Rat ImmunoPhenomic Facility (TRIP), Nantes, France.; MARCELA SOUZA-NEVES, Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay.; JEAN-MARIE HESLAN, INSERM Centre de Recherche en Transplantation et Immunologie UMR 1064, Transgenesis Rat ImmunoPhenomic Facility (TRIP), Nantes, France.; FEDERICO CUADRO, Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay.; IGNACIO ANEGÓN, INSERM Centre de Recherche en Transplantation et Immunologie UMR 1064, Transgenesis Rat ImmunoPhenomic Facility (TRIP), Nantes, France.; JOSE ALEJO MENCHACA BARBEITO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay./Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay. |
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Título : |
Generation of a Human Deafness Sheep Model Using the CRISPR/Cas System. |
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Complemento del título : |
Chapter 12. |
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Fecha de publicación : |
2022 |
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Fuente / Imprenta : |
Methods in Molecular Biology, 2022, Volume 2495, Pages 233-258. doi: https://doi.org/10.1007/978-1-0716-2301-5_12 |
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DOI : |
10.1007/978-1-0716-2301-5_12 |
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Idioma : |
Inglés |
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Contenido : |
Abstract:
CRISPR/Cas9 system is a promising method for the generation of human disease models by genome editing in non-conventional experimental animals. Medium/large-sized animals like sheep have several advantages to study human diseases and medicine. Here, we present a protocol that describes the generation of an otoferlin edited sheep model via CRISPR-assisted single-stranded oligodinucleotide-mediated Homology-Directed Repair (HDR), through direct cytoplasmic microinjection in in vitro produced zygotes.Otoferlin is a protein expressed in the cochlear inner hair cells, with different mutations at the OTOF gene being the major cause of nonsyndromic recessive auditory neuropathy spectrum disorder in humans. By using this protocol, we reported for the first time an OTOF KI model in sheep with 17.8% edited lambs showing indel mutations, and 61.5% of them bearing knock-in mutations by HDR . The reported method establishes the bases to produce a deafness model to test novel therapies in human disorders related to OTOF mutations. |
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Palabras claves : |
Embryo; Genome editing; In vitro fertilization; OTOF; SsODNs. |
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Thesagro : |
OVEJA. |
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Asunto categoría : |
-- |
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Marc : |
LEADER 01915naa a2200301 a 4500
001 1063442
005 2022-07-20
008 2022 bl uuuu u00u1 u #d
024 7 $a10.1007/978-1-0716-2301-5_12$2DOI
100 1 $aCRISPO, M.
245 $aGeneration of a Human Deafness Sheep Model Using the CRISPR/Cas System.$h[electronic resource]
260 $c2022
520 $aAbstract: CRISPR/Cas9 system is a promising method for the generation of human disease models by genome editing in non-conventional experimental animals. Medium/large-sized animals like sheep have several advantages to study human diseases and medicine. Here, we present a protocol that describes the generation of an otoferlin edited sheep model via CRISPR-assisted single-stranded oligodinucleotide-mediated Homology-Directed Repair (HDR), through direct cytoplasmic microinjection in in vitro produced zygotes.Otoferlin is a protein expressed in the cochlear inner hair cells, with different mutations at the OTOF gene being the major cause of nonsyndromic recessive auditory neuropathy spectrum disorder in humans. By using this protocol, we reported for the first time an OTOF KI model in sheep with 17.8% edited lambs showing indel mutations, and 61.5% of them bearing knock-in mutations by HDR . The reported method establishes the bases to produce a deafness model to test novel therapies in human disorders related to OTOF mutations.
650 $aOVEJA
653 $aEmbryo
653 $aGenome editing
653 $aIn vitro fertilization
653 $aOTOF
653 $aSsODNs
700 1 $aCHENOUARD, V.
700 1 $aDOS SANTOS-NETO P
700 1 $aTESSON, L.
700 1 $aSOUZA-NEVES, M.
700 1 $aHESLAN, J.M.
700 1 $aCUADRO, F.
700 1 $aANEGÓN, I.
700 1 $aMENCHACA, A.
773 $tMethods in Molecular Biology, 2022, Volume 2495, Pages 233-258. doi: https://doi.org/10.1007/978-1-0716-2301-5_12
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Registro original : |
INIA La Estanzuela (LE) |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha actual : |
26/02/2021 |
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Actualizado : |
10/08/2021 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
BAETHGEN, W.E.; PARTON, W.P-; RUBIO, V.; KELLY, R.H.; LUTZ, S. |
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Afiliación : |
WALTER E. BAETHGEN, International Research Institute for Climate and Society, The Earth Institute, Columbia University, New York, USA.; WILLIAM J. PARTON, Natural Resource Ecology Lab, Colorado State University, Fort Collins, Colorado, USA.; VALENTINA RUBIO DELLEPIANE, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ROBIN H. KELLY, Natural Resource Ecology Lab, Colorado State University, Fort Collins, Colorado, USA.; SUSAN LUTZ, Natural Resource Ecology Lab, Colorado State University, Fort Collins, Colorado, USA. |
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Título : |
Ecosystem dynamics of crop-pasture rotations in a fifty-year field experiment in Southern South America: Century model and field results. |
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Fecha de publicación : |
2021 |
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Fuente / Imprenta : |
Soil Science Society of America Journal, Volume 85, Issue 2, Pages 423-437, March/April 2021. DOI: https://doi.org/10.1002/saj2.20204 |
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DOI : |
doi.org/10.1002/saj2.20204 |
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Idioma : |
Inglés |
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Notas : |
Article History: Accepted manuscript online: 26 November 2020; Manuscript accepted: 18 November 2020; Manuscript revised: 02 November 2020 ; Manuscript received: 12 February 2020; Published online: 24 March 2021. |
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Contenido : |
Abstract:
The Century model was used to simulate soil carbon (C) and nitrogen (N) cycling, and crop production dynamics in an ongoing field experiment in Uruguay (started in 1963). The model was calibrated using observed data from three treatments (crop or crop?pasture rotations) and validated with a fourth treatment. The model correctly predicted the impact of different treatments on microbial biomass, N mineralization, soil respiration, and crop yields. The model and observed data show that soil respiration, N mineralization, soil C, and crop yields increase with increasing plant derived C inputs caused by increasing the frequency of pastures in the rotations. This is one of the first papers which show the strong positive correlation of observed soil C with plant C soil inputs to field?observed microbial biomass, soil respiration, and N mineralization. The results also showed that reducing tillage and transitioning to a no?till system increased soil C and reduced soil erosion. The main path of soil C losses was heterotrophic microbial respiration which accounted for 66% of the total C lost in a continuous crop rotation and no fertilizers, 71% in a continuous crop rotation with fertilizers, and 86% in a crop?pasture rotation with fertilizers. Model results from a degraded cropping system showed that adding grass/clover pastures greatly increased plant production and soil C, while reducing the frequency of grass/clover pastures in high?fertility cropping systems from 50% of the time to 25% reduces crop yields and soil C. Including cover crops substantially increases crop production and maintains soil C in high fertility and degraded cropping systems MenosAbstract:
The Century model was used to simulate soil carbon (C) and nitrogen (N) cycling, and crop production dynamics in an ongoing field experiment in Uruguay (started in 1963). The model was calibrated using observed data from three treatments (crop or crop?pasture rotations) and validated with a fourth treatment. The model correctly predicted the impact of different treatments on microbial biomass, N mineralization, soil respiration, and crop yields. The model and observed data show that soil respiration, N mineralization, soil C, and crop yields increase with increasing plant derived C inputs caused by increasing the frequency of pastures in the rotations. This is one of the first papers which show the strong positive correlation of observed soil C with plant C soil inputs to field?observed microbial biomass, soil respiration, and N mineralization. The results also showed that reducing tillage and transitioning to a no?till system increased soil C and reduced soil erosion. The main path of soil C losses was heterotrophic microbial respiration which accounted for 66% of the total C lost in a continuous crop rotation and no fertilizers, 71% in a continuous crop rotation with fertilizers, and 86% in a crop?pasture rotation with fertilizers. Model results from a degraded cropping system showed that adding grass/clover pastures greatly increased plant production and soil C, while reducing the frequency of grass/clover pastures in high?fertility cropping systems from 50% of ... Presentar Todo |
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Palabras claves : |
CROP PASTURE ROTATION; CROPPING SYSTEMS; GRASS-CLOVER PASTURES; HIGH FERTILITY. |
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Thesagro : |
CICLO DEL CARBONO; CICLO DEL NITROGENO; ECOSISTEMAS; SUELOS. |
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Asunto categoría : |
-- |
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Marc : |
LEADER 02844naa a2200289 a 4500
001 1061775
005 2021-08-10
008 2021 bl uuuu u00u1 u #d
024 7 $adoi.org/10.1002/saj2.20204$2DOI
100 1 $aBAETHGEN, W.E.
245 $aEcosystem dynamics of crop-pasture rotations in a fifty-year field experiment in Southern South America$bCentury model and field results.$h[electronic resource]
260 $c2021
500 $aArticle History: Accepted manuscript online: 26 November 2020; Manuscript accepted: 18 November 2020; Manuscript revised: 02 November 2020 ; Manuscript received: 12 February 2020; Published online: 24 March 2021.
520 $aAbstract: The Century model was used to simulate soil carbon (C) and nitrogen (N) cycling, and crop production dynamics in an ongoing field experiment in Uruguay (started in 1963). The model was calibrated using observed data from three treatments (crop or crop?pasture rotations) and validated with a fourth treatment. The model correctly predicted the impact of different treatments on microbial biomass, N mineralization, soil respiration, and crop yields. The model and observed data show that soil respiration, N mineralization, soil C, and crop yields increase with increasing plant derived C inputs caused by increasing the frequency of pastures in the rotations. This is one of the first papers which show the strong positive correlation of observed soil C with plant C soil inputs to field?observed microbial biomass, soil respiration, and N mineralization. The results also showed that reducing tillage and transitioning to a no?till system increased soil C and reduced soil erosion. The main path of soil C losses was heterotrophic microbial respiration which accounted for 66% of the total C lost in a continuous crop rotation and no fertilizers, 71% in a continuous crop rotation with fertilizers, and 86% in a crop?pasture rotation with fertilizers. Model results from a degraded cropping system showed that adding grass/clover pastures greatly increased plant production and soil C, while reducing the frequency of grass/clover pastures in high?fertility cropping systems from 50% of the time to 25% reduces crop yields and soil C. Including cover crops substantially increases crop production and maintains soil C in high fertility and degraded cropping systems
650 $aCICLO DEL CARBONO
650 $aCICLO DEL NITROGENO
650 $aECOSISTEMAS
650 $aSUELOS
653 $aCROP PASTURE ROTATION
653 $aCROPPING SYSTEMS
653 $aGRASS-CLOVER PASTURES
653 $aHIGH FERTILITY
700 1 $aPARTON, W.P-
700 1 $aRUBIO, V.
700 1 $aKELLY, R.H.
700 1 $aLUTZ, S.
773 $tSoil Science Society of America Journal, Volume 85, Issue 2, Pages 423-437, March/April 2021. DOI: https://doi.org/10.1002/saj2.20204
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