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 | Acceso al texto completo restringido a Biblioteca INIA Treinta y Tres. Por información adicional contacte bibliott@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Treinta y Tres. |
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Fecha : |
03/12/2020 |
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Actualizado : |
03/12/2020 |
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Autor : |
NARANCIO, R.; DING, Y.-L.; LIN, Y.-H.; SAHAB, S.; PANTER, S.; HAYES, M.; JOHN, U.; ANDERSON, H.; MASON, J.; SPANGENBERG, G. |
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Afiliación : |
RAFAEL NARANCIO FERES, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; Agriculture Victoria Research, AgriBio, Centre for AgriBioscience, Bundoora, Australia; School of Applied Systems Biology, La Trobe University, Bundoora, Australia; YONG?LIN DING, Agriculture Victoria Research, AgriBio, Centre for AgriBioscience, Bundoora, Australia; YI?HAN LIN, Agriculture Victoria Research, AgriBio, Centre for AgriBioscience, Bundoora, Australia; SAREENA SAHAB, Agriculture Victoria Research, AgriBio, Centre for AgriBioscience, Bundoora, Australia; STEPHEN PANTER, Agriculture Victoria Research, AgriBio, Centre for AgriBioscience, Bundoora, Australia; MATTHEW HAYES, Triffid BioScience, PO Box 1986, Carlton South, Australia; ULRIK JOHN, Agriculture Victoria Research, AgriBio, Centre for AgriBioscience, Bundoora, Australia; HEATHER ANDERSON, Agriculture Victoria Research, AgriBio, Centre for AgriBioscience, Bundoora, Australia; JOHN MASON, Agriculture Victoria Research, AgriBio, Centre for AgriBioscience, Bundoora, Australia; School of Applied Systems Biology, La Trobe University, Bundoora, Australia; GERMAN SPANGENBERG, Agriculture Victoria Research, AgriBio, Centre for AgriBioscience, Bundoora, Australia; School of Applied Systems Biology, La Trobe University, Bundoora, Australia. |
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Título : |
Correction to: Application of linked and unlinked co‑transformation to generate triple stack, marker‑free, transgenic white clover (Trifolium repens L.) (Plant Cell, Tissue and Organ Culture (PCTOC), (2020), 142, 3, (635-646), 10.1007/s11240-020-01891-6). |
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Fecha de publicación : |
2020 |
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Fuente / Imprenta : |
Plant Cell, Tissue and Organ Culture, 2020. DOI: https://doi.org/10.1007/s11240-020-01891-6 |
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DOI : |
10.1007/s11240-020-01891-6 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: 19 May 2020 / Accepted: 15 July 2020. |
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Contenido : |
ABSTRACT.
Both linked and un-linked co-transformation can be used to overcome limitations of methods, such as re-transformation or sexual crossing of transgenic plants, to enable transfer of multiple genes to a single plant. Un-linked co-transformation can also facilitate the production of selectable marker-free transgenic plants. In this study, transgenic white clover plants were generated by Agrobacterium-mediated linked co-transformation using a single T-DNA of 9803 bp expressing: an isopentenyl transferase (IPT) gene for delayed leaf senescence under the control of an organ specific MYB32 promoter from Arabidopsis, a white clover nodule enhanced malate dehydrogenase (neMDH) gene for aluminium tolerance controlled by the endogenous Phosphate Transporter 1 (PT1) promoter, and the coat protein gene from Alfalfa Mosaic Virus (CP-AMV) controlled by the 35S promoter from Cauliflower Mosaic Virus. The selectable marker gene encoding hygromycin phosphotransferase (hph) was borne on a separate T-DNA. Forty independent transgenic events carrying the triple stack were generated, with estimated co-transformation efficiencies of 0.22 to 0.23%. Forty three percent of the events generated had a single insertion, while two events were selectable marker-free. Transcript abundance studies of the three transgenes of interest demonstrated the transcriptional competence of the inserted T-DNA. This study illustrates the feasibility of transferring multiple genes in a large single T-DNA into white clover by Agrobacterium-mediated co-transformation. Furthermore, observations of consistently delayed leaf senescence, statistically significant increases in TrneMDH transcript, and presence of CP-AMV transcript, support further analysis of these events for delayed leaf senescence under drought conditions, aluminium tolerance, and resistance to AMV.
© 2020, Springer Nature B.V. MenosABSTRACT.
Both linked and un-linked co-transformation can be used to overcome limitations of methods, such as re-transformation or sexual crossing of transgenic plants, to enable transfer of multiple genes to a single plant. Un-linked co-transformation can also facilitate the production of selectable marker-free transgenic plants. In this study, transgenic white clover plants were generated by Agrobacterium-mediated linked co-transformation using a single T-DNA of 9803 bp expressing: an isopentenyl transferase (IPT) gene for delayed leaf senescence under the control of an organ specific MYB32 promoter from Arabidopsis, a white clover nodule enhanced malate dehydrogenase (neMDH) gene for aluminium tolerance controlled by the endogenous Phosphate Transporter 1 (PT1) promoter, and the coat protein gene from Alfalfa Mosaic Virus (CP-AMV) controlled by the 35S promoter from Cauliflower Mosaic Virus. The selectable marker gene encoding hygromycin phosphotransferase (hph) was borne on a separate T-DNA. Forty independent transgenic events carrying the triple stack were generated, with estimated co-transformation efficiencies of 0.22 to 0.23%. Forty three percent of the events generated had a single insertion, while two events were selectable marker-free. Transcript abundance studies of the three transgenes of interest demonstrated the transcriptional competence of the inserted T-DNA. This study illustrates the feasibility of transferring multiple genes in a large single T-DNA into w... Presentar Todo |
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Palabras claves : |
Agrobacterium-mediated transformation; Delayed leaf senescence; Multigene; T-DNA. |
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Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
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Marc : |
LEADER 03007naa a2200301 a 4500
001 1061539
005 2020-12-03
008 2020 bl uuuu u00u1 u #d
024 7 $a10.1007/s11240-020-01891-6$2DOI
100 1 $aNARANCIO, R.
245 $aCorrection to$bApplication of linked and unlinked co‑transformation to generate triple stack, marker‑free, transgenic white clover (Trifolium repens L.) (Plant Cell, Tissue and Organ Culture (PCTOC), (2020), 142, 3, (635-646), 10.1007/s11240-020-01891-6).$h[electronic resource]
260 $c2020
500 $aArticle history: Received: 19 May 2020 / Accepted: 15 July 2020.
520 $aABSTRACT. Both linked and un-linked co-transformation can be used to overcome limitations of methods, such as re-transformation or sexual crossing of transgenic plants, to enable transfer of multiple genes to a single plant. Un-linked co-transformation can also facilitate the production of selectable marker-free transgenic plants. In this study, transgenic white clover plants were generated by Agrobacterium-mediated linked co-transformation using a single T-DNA of 9803 bp expressing: an isopentenyl transferase (IPT) gene for delayed leaf senescence under the control of an organ specific MYB32 promoter from Arabidopsis, a white clover nodule enhanced malate dehydrogenase (neMDH) gene for aluminium tolerance controlled by the endogenous Phosphate Transporter 1 (PT1) promoter, and the coat protein gene from Alfalfa Mosaic Virus (CP-AMV) controlled by the 35S promoter from Cauliflower Mosaic Virus. The selectable marker gene encoding hygromycin phosphotransferase (hph) was borne on a separate T-DNA. Forty independent transgenic events carrying the triple stack were generated, with estimated co-transformation efficiencies of 0.22 to 0.23%. Forty three percent of the events generated had a single insertion, while two events were selectable marker-free. Transcript abundance studies of the three transgenes of interest demonstrated the transcriptional competence of the inserted T-DNA. This study illustrates the feasibility of transferring multiple genes in a large single T-DNA into white clover by Agrobacterium-mediated co-transformation. Furthermore, observations of consistently delayed leaf senescence, statistically significant increases in TrneMDH transcript, and presence of CP-AMV transcript, support further analysis of these events for delayed leaf senescence under drought conditions, aluminium tolerance, and resistance to AMV. © 2020, Springer Nature B.V.
653 $aAgrobacterium-mediated transformation
653 $aDelayed leaf senescence
653 $aMultigene
653 $aT-DNA
700 1 $aDING, Y.-L.
700 1 $aLIN, Y.-H.
700 1 $aSAHAB, S.
700 1 $aPANTER, S.
700 1 $aHAYES, M.
700 1 $aJOHN, U.
700 1 $aANDERSON, H.
700 1 $aMASON, J.
700 1 $aSPANGENBERG, G.
773 $tPlant Cell, Tissue and Organ Culture, 2020. DOI: https://doi.org/10.1007/s11240-020-01891-6
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Registro original : |
INIA Treinta y Tres (TT) |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas; INIA Tacuarembó. |
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Fecha actual : |
21/02/2014 |
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Actualizado : |
03/02/2018 |
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Tipo de producción científica : |
Capítulo en Libro Técnico-Científico |
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Autor : |
CASTAÑO, J.; GIMÉNEZ, A.; OLIVERA, L.; FUREST, J.; PICOS, C. |
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Afiliación : |
JOSE PEDRO CASTAÑO SANCHEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; AGUSTIN EDUARDO GIMÉNEZ FUREST, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; LAURA OLIVERA MC ALISTER, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JOSE MARIA FUREST CROCCO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CARLOS PICOS ALONSO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Caracterización agro climática y monitoreo del cambio de uso del suelo en la región de areniscas de Tacuarembó |
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Fecha de publicación : |
2006 |
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Fuente / Imprenta : |
ln: Bemhaja, M.; Pittaluga, O., eds. 30 años de investigación en suelos de areniscas, INIA Tacuarembó. Montevideo (Uruguay): INIA, 2006. |
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Páginas : |
p. 1-7 |
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Serie : |
(INIA Serie Técnica ; 159) |
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ISSN : |
1688-9266 |
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Idioma : |
Español |
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Contenido : |
La región Areniscas de Tacuarembó (Zona 7, CIDE), se caracteriza por estar desarrollada sobre areniscas-gris amarillentas y rojas de Tacuarembó, siendo los sue- los profundos, de textura liviana y alta disponibilidad de agua pero de baja fertilidad. La capacidad de retener agua de los suelos de esta región es muy alta. Las dos unidades de suelos predominantes (Cartografía 1.000.000): Tacuarembó y Rivera, tienen una capacidad de 168 y 179 mm respectivamente. La producción de forraje de las pasturas naturales de esta región no es despreciable, pero presenta como dificultad para su manejo la marcada estacionalidad, concentrando la producción en primavera - verano y con un período de baja producción en otoño- invierno. |
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Thesagro : |
BALANCE HIDRICO; EVAPORACION; FACTORES CLIMATICOS; LLUVIA; SUELO ARENOSO; TEMPERATURA DEL AIRE; URUGUAY. |
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Asunto categoría : |
--
P30 Ciencia del suelo y manejo del suelo |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/7496/1/ST-159-1-7..pdf
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Marc : |
LEADER 01625naa a2200289 a 4500
001 1007929
005 2018-02-03
008 2006 bl uuuu u00u1 u #d
022 $a1688-9266
100 1 $aCASTAÑO, J.
245 $aCaracterización agro climática y monitoreo del cambio de uso del suelo en la región de areniscas de Tacuarembó
260 $c2006
300 $ap. 1-7
490 $a(INIA Serie Técnica ; 159)
520 $aLa región Areniscas de Tacuarembó (Zona 7, CIDE), se caracteriza por estar desarrollada sobre areniscas-gris amarillentas y rojas de Tacuarembó, siendo los sue- los profundos, de textura liviana y alta disponibilidad de agua pero de baja fertilidad. La capacidad de retener agua de los suelos de esta región es muy alta. Las dos unidades de suelos predominantes (Cartografía 1.000.000): Tacuarembó y Rivera, tienen una capacidad de 168 y 179 mm respectivamente. La producción de forraje de las pasturas naturales de esta región no es despreciable, pero presenta como dificultad para su manejo la marcada estacionalidad, concentrando la producción en primavera - verano y con un período de baja producción en otoño- invierno.
650 $aBALANCE HIDRICO
650 $aEVAPORACION
650 $aFACTORES CLIMATICOS
650 $aLLUVIA
650 $aSUELO ARENOSO
650 $aTEMPERATURA DEL AIRE
650 $aURUGUAY
700 1 $aGIMÉNEZ, A.
700 1 $aOLIVERA, L.
700 1 $aFUREST, J.
700 1 $aPICOS, C.
773 $tln: Bemhaja, M.; Pittaluga, O., eds. 30 años de investigación en suelos de areniscas, INIA Tacuarembó. Montevideo (Uruguay): INIA, 2006.
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