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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
29/07/2020 |
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Actualizado : |
03/12/2020 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
NARANCIO, R.; DING, Y.-L.; LIN, Y.-H.; SAHAB, S.; PANTER, S.; HAYES, M.; JOHN, U.; ANDERSON, H.; MASON, J.; SPANGENBERG, G. |
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Afiliación : |
RAFAEL NARANCIO FERES, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; Agriculture Victoria Research, AgriBio, Centre for AgriBioscience, Bundoora, Australia; School of Applied Systems Biology, La Trobe University, Bundoora, Australia; YONG?LIN DING, Agriculture Victoria Research, AgriBio, Centre for AgriBioscience, Bundoora, Australia; YI?HAN LIN, Agriculture Victoria Research, AgriBio, Centre for AgriBioscience, Bundoora, Australia; SAREENA SAHAB, Agriculture Victoria Research, AgriBio, Centre for AgriBioscience, Bundoora, Australia; STEPHEN PANTER, Agriculture Victoria Research, AgriBio, Centre for AgriBioscience, Bundoora, Australia; MATTHEW HAYES, Triffid BioScience, PO Box 1986, Carlton South, Australia; ULRIK JOHN, Agriculture Victoria Research, AgriBio, Centre for AgriBioscience, Bundoora, Australia; HEATHER ANDERSON, Agriculture Victoria Research, AgriBio, Centre for AgriBioscience, Bundoora, Australia; JOHN MASON, Agriculture Victoria Research, AgriBio, Centre for AgriBioscience, Bundoora, Australia; School of Applied Systems Biology, La Trobe University, Bundoora, Australia; GERMAN SPANGENBERG, Agriculture Victoria Research, AgriBio, Centre for AgriBioscience, Bundoora, Australia; School of Applied Systems Biology, La Trobe University, Bundoora, Australia. |
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Título : |
Application of linked and unlinked co transformation to generate triple stack, marker free, transgenic white clover (Trifolium repens L.). |
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Fecha de publicación : |
2020 |
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Fuente / Imprenta : |
Plant Cell, Tissue and Organ Culture, 2020. DOI: https://doi.org/10.1007/s11240-020-01891-6 |
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ISSN : |
0167-6857 |
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DOI : |
10.1007/s11240-020-01891-6 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: 19 May 2020 / Accepted: 15 July 2020. |
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Contenido : |
ABSTRACT.
Both linked and un-linked co-transformation can be used to overcome limitations of methods, such as re-transformation or sexual crossing of transgenic plants, to enable transfer of multiple genes to a single plant. Un-linked co-transformation can also facilitate the production of selectable marker-free transgenic plants. In this study, transgenic white clover plants were generated by Agrobacterium-mediated linked co-transformation using a single T-DNA of 9803 bp expressing: an isopentenyl transferase (IPT) gene for delayed leaf senescence under the control of an organ specific MYB32 promoter from Arabidopsis, a white clover nodule enhanced malate dehydrogenase (neMDH) gene for aluminium tolerance controlled by the endogenous Phosphate Transporter 1 (PT1) promoter, and the coat protein gene from Alfalfa Mosaic Virus (CP-AMV) controlled by the 35S promoter from Cauliflower Mosaic Virus. The selectable marker gene encoding hygromycin phosphotransferase (hph) was borne on a separate T-DNA. Forty independent transgenic events carrying the triple stack were generated, with estimated co-transformation efficiencies of 0.22 to 0.23%. Forty three percent of the events generated had a single insertion, while two events were selectable marker-free. Transcript abundance studies of the three transgenes of interest demonstrated the transcriptional competence of the inserted T-DNA. This study illustrates the feasibility of transferring multiple genes in a large single T-DNA into white clover by Agrobacterium-mediated co-transformation. Furthermore, observations of consistently delayed leaf senescence, statistically significant increases in TrneMDH transcript, and presence of CP-AMV transcript, support further analysis of these events for delayed leaf senescence under drought conditions, aluminium tolerance, and resistance to AMV.
© 2020, Springer Nature B.V. MenosABSTRACT.
Both linked and un-linked co-transformation can be used to overcome limitations of methods, such as re-transformation or sexual crossing of transgenic plants, to enable transfer of multiple genes to a single plant. Un-linked co-transformation can also facilitate the production of selectable marker-free transgenic plants. In this study, transgenic white clover plants were generated by Agrobacterium-mediated linked co-transformation using a single T-DNA of 9803 bp expressing: an isopentenyl transferase (IPT) gene for delayed leaf senescence under the control of an organ specific MYB32 promoter from Arabidopsis, a white clover nodule enhanced malate dehydrogenase (neMDH) gene for aluminium tolerance controlled by the endogenous Phosphate Transporter 1 (PT1) promoter, and the coat protein gene from Alfalfa Mosaic Virus (CP-AMV) controlled by the 35S promoter from Cauliflower Mosaic Virus. The selectable marker gene encoding hygromycin phosphotransferase (hph) was borne on a separate T-DNA. Forty independent transgenic events carrying the triple stack were generated, with estimated co-transformation efficiencies of 0.22 to 0.23%. Forty three percent of the events generated had a single insertion, while two events were selectable marker-free. Transcript abundance studies of the three transgenes of interest demonstrated the transcriptional competence of the inserted T-DNA. This study illustrates the feasibility of transferring multiple genes in a large single T-DNA into w... Presentar Todo |
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Palabras claves : |
Agrobacterium-mediated transformation; Delayed leaf senescence; Multigene; T-DNA. |
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Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
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Marc : |
LEADER 02904naa a2200313 a 4500
001 1061249
005 2020-12-03
008 2020 bl uuuu u00u1 u #d
022 $a0167-6857
024 7 $a10.1007/s11240-020-01891-6$2DOI
100 1 $aNARANCIO, R.
245 $aApplication of linked and unlinked co transformation to generate triple stack, marker free, transgenic white clover (Trifolium repens L.).$h[electronic resource]
260 $c2020
500 $aArticle history: Received: 19 May 2020 / Accepted: 15 July 2020.
520 $aABSTRACT. Both linked and un-linked co-transformation can be used to overcome limitations of methods, such as re-transformation or sexual crossing of transgenic plants, to enable transfer of multiple genes to a single plant. Un-linked co-transformation can also facilitate the production of selectable marker-free transgenic plants. In this study, transgenic white clover plants were generated by Agrobacterium-mediated linked co-transformation using a single T-DNA of 9803 bp expressing: an isopentenyl transferase (IPT) gene for delayed leaf senescence under the control of an organ specific MYB32 promoter from Arabidopsis, a white clover nodule enhanced malate dehydrogenase (neMDH) gene for aluminium tolerance controlled by the endogenous Phosphate Transporter 1 (PT1) promoter, and the coat protein gene from Alfalfa Mosaic Virus (CP-AMV) controlled by the 35S promoter from Cauliflower Mosaic Virus. The selectable marker gene encoding hygromycin phosphotransferase (hph) was borne on a separate T-DNA. Forty independent transgenic events carrying the triple stack were generated, with estimated co-transformation efficiencies of 0.22 to 0.23%. Forty three percent of the events generated had a single insertion, while two events were selectable marker-free. Transcript abundance studies of the three transgenes of interest demonstrated the transcriptional competence of the inserted T-DNA. This study illustrates the feasibility of transferring multiple genes in a large single T-DNA into white clover by Agrobacterium-mediated co-transformation. Furthermore, observations of consistently delayed leaf senescence, statistically significant increases in TrneMDH transcript, and presence of CP-AMV transcript, support further analysis of these events for delayed leaf senescence under drought conditions, aluminium tolerance, and resistance to AMV. © 2020, Springer Nature B.V.
653 $aAgrobacterium-mediated transformation
653 $aDelayed leaf senescence
653 $aMultigene
653 $aT-DNA
700 1 $aDING, Y.-L.
700 1 $aLIN, Y.-H.
700 1 $aSAHAB, S.
700 1 $aPANTER, S.
700 1 $aHAYES, M.
700 1 $aJOHN, U.
700 1 $aANDERSON, H.
700 1 $aMASON, J.
700 1 $aSPANGENBERG, G.
773 $tPlant Cell, Tissue and Organ Culture, 2020. DOI: https://doi.org/10.1007/s11240-020-01891-6
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| 2. |  | DIAZ, P.; CABRERA, A.; ALEM, D.; LARRAÑAGA, P.; FERREIRA, F.; DALLA RIZZA, M. Antifungal activity of medicinal plant extracts against phytopathogenic fungus Alternaria spp. [Actividad antifúngica de extractos de plantas medicinales contra el hongo fitopatógeno alternaria spp.] Chilean Journal of Agricultural Research, 2011, v.71, no.2, p.231-239. doi: http://dx.doi.org/10.4067/S0718-58392011000200008 2-s2.0-79960343579 RESEARCH. Received: 2 August 2010. Accepted: 26 December 2010.| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : B - 3 |
| Biblioteca(s): INIA Las Brujas. |
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| 3. | | ALEM, D.; NARANCIO, R.; DIAZ, P.; REBUFFO, M.; ZARZA, R.; DALLA RIZZA, M. Molecular characterization of Lotus corniculatus cultivars using transferable microsatellite markers. Ciencia e Investigación Agraria, Santiago de Chile, v. 38, n. 3, p. 463-471, 2011. Article history: Received September 7 2010. /Accepted June 21, 2011.| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : B - 3 |
| Biblioteca(s): INIA La Estanzuela. |
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| 4. | | LARRAÑAGA, P.; DIAZ, P.; CABRERA, A.; ALEM, D.; ALMEIDA SOUZA, A.L.; GIOVANNI DE SIMONE, S.; DALLA RIZZA, M. Activity of naturally derived antimicrobial peptides against filamentous fungi relevant for agriculture . Sustainable Agriculture Research, 2012, v. 1, no.2, p. 211-221. Article history: Received: June 12, 2012 / Accepted: July 10, 2012 / Online Published: July 23, 2012.| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : C - 0 |
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| 5. | | ALEM, D.; DIAZ, P.; LEONI, C.; DE SIMONE, S.G.; CORREA, A.; OPPEZZO, P.; DALLA RIZZA, M. In Search of Topical Agricultural Biofungicides: Properties of the Recombinant Antimicrobial Peptide TrxAq-AMP obtained from Amaranthus quitensis. Journal of Microbial & Biochemical Technology, 2014, v. 6, no.5, p. 268-273.| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : B - 4 |
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