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| Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
27/01/2020 |
Actualizado : |
29/05/2020 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
BERNASCHINA, Y.; LEONI, C.; ALANIZ, S. |
Afiliación : |
YESICA STEFANIA BERNASCHINA CORREA, Departamento de Protección Vegetal, Fitopatología, Facultad de Agronomía, Universidad de la República, Uruguay; INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CAROLINA LEONI VELAZCO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; SANDRA ALANIZ, Departamento de Protección Vegetal, Fitopatología, Facultad de Agronomía, Universidad de la República, Montevideo, Uruguay. |
Título : |
Genetic diversity evidence a mixed reproduction mode in Venturia oleaginea populations in Uruguay. |
Fecha de publicación : |
2020 |
Fuente / Imprenta : |
Journal of Plant Pathology, 1 February 2020, Volume 102, Issue 1, Pages 123-133. Doi: https://doi.org/10.1007/s42161-019-00396-2 |
ISSN : |
1125-4653 |
DOI : |
10.1007/s42161-019-00396-2 |
Idioma : |
Inglés |
Notas : |
Article history: Received: 15 June 2018 / Accepted: 3 September 2019 / Published online: 17 October 2019.
Funding text: This research was funded by The National Institute of Agricultural Research (INIA - Uruguay). The first author granted a scholarship from The National Agency for Research and Innovation, Uruguay (ANII grant POS_NAC_2014_1_102182) and from UdelaR to carry out this research as part of her Master Degree. The authors are grateful to Dr. Guillermo Perez for his substantial contributions along the research process, particularly in analysis and discussion of genetic diversity data. Also we are grateful to Paula Conde and Jose Villamil for their support in contacting the surveyed orchards and to the owners of olive orchards, and to Pedro Mondino for his assistance in obtaining monoconidial isolates. Partial results were presented at XXV Congress Sociedad Chilena de Fitopatología ? XIX Congreso Asociación Latinoamericana de Fitopatología y LVII APS Caribbean Division Meeting, Termas de Chillán, Chile, 2-5 October, 2017. Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. |
Contenido : |
ABSTRACT.
Olive scab caused by Venturia oleaginea is one of the most important diseases of olive worldwide. The fungus infects young leaves and fruits by asexually produced conidia. The sexual stage and its importance in the epidemiology and genetics of the pathogen population is unknown. A collection of 52 fungal isolates was obtained from scabbed leaves collected in 16 orchards from three major regions in Uruguay. All isolates were identified as V. oleaginea by PCR with specific primers and sequence analysis of the TEF-1α gene region. Five colony morphotypes and different growing rates were observed on potato dextrose agar (PDA) medium. Universally primed polymerase chain reaction (UP-PCR) was successfully used for assessing the genetic diversity and population structure of the V. oleaginea isolates, whereas ISSR was unable to generate reproducible and polymorphic bands. Two populations genetically different were identified: [1] composed by 21 isolates mainly from Southern and South-Western regions; [2] composed by 31 isolates mainly from South-Eastern and Southern regions. Both populations showed a moderate gene diversity expressed as hNei = 0.163 and 0.212 for population [1] and [2], respectively. There was evidence of linkage disequilibrium in both populations, rejecting the null hypothesis of random mating as the predominant reproduction mode, a typical result of clonal populations. However, all isolates were genotypically different, an indirect evidence of recombination. We conclude that V. oleaginea populations in Uruguay present a mixed mode of reproduction, a characteristic of many pathogens that are difficult to control.
© 2019, Società Italiana di Patologia Vegetale (S.I.Pa.V.). MenosABSTRACT.
Olive scab caused by Venturia oleaginea is one of the most important diseases of olive worldwide. The fungus infects young leaves and fruits by asexually produced conidia. The sexual stage and its importance in the epidemiology and genetics of the pathogen population is unknown. A collection of 52 fungal isolates was obtained from scabbed leaves collected in 16 orchards from three major regions in Uruguay. All isolates were identified as V. oleaginea by PCR with specific primers and sequence analysis of the TEF-1α gene region. Five colony morphotypes and different growing rates were observed on potato dextrose agar (PDA) medium. Universally primed polymerase chain reaction (UP-PCR) was successfully used for assessing the genetic diversity and population structure of the V. oleaginea isolates, whereas ISSR was unable to generate reproducible and polymorphic bands. Two populations genetically different were identified: [1] composed by 21 isolates mainly from Southern and South-Western regions; [2] composed by 31 isolates mainly from South-Eastern and Southern regions. Both populations showed a moderate gene diversity expressed as hNei = 0.163 and 0.212 for population [1] and [2], respectively. There was evidence of linkage disequilibrium in both populations, rejecting the null hypothesis of random mating as the predominant reproduction mode, a typical result of clonal populations. However, all isolates were genotypically different, an indirect evidence of recomb... Presentar Todo |
Palabras claves : |
Fusicladium oleagineum; Genetic diversity; Olive scab. |
Thesagro : |
OLEA EUROPAEA; OLIVOS. |
Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
Marc : |
LEADER 03666naa a2200241 a 4500 001 1060692 005 2020-05-29 008 2020 bl uuuu u00u1 u #d 022 $a1125-4653 024 7 $a10.1007/s42161-019-00396-2$2DOI 100 1 $aBERNASCHINA, Y. 245 $aGenetic diversity evidence a mixed reproduction mode in Venturia oleaginea populations in Uruguay.$h[electronic resource] 260 $c2020 500 $aArticle history: Received: 15 June 2018 / Accepted: 3 September 2019 / Published online: 17 October 2019. Funding text: This research was funded by The National Institute of Agricultural Research (INIA - Uruguay). The first author granted a scholarship from The National Agency for Research and Innovation, Uruguay (ANII grant POS_NAC_2014_1_102182) and from UdelaR to carry out this research as part of her Master Degree. The authors are grateful to Dr. Guillermo Perez for his substantial contributions along the research process, particularly in analysis and discussion of genetic diversity data. Also we are grateful to Paula Conde and Jose Villamil for their support in contacting the surveyed orchards and to the owners of olive orchards, and to Pedro Mondino for his assistance in obtaining monoconidial isolates. Partial results were presented at XXV Congress Sociedad Chilena de Fitopatología ? XIX Congreso Asociación Latinoamericana de Fitopatología y LVII APS Caribbean Division Meeting, Termas de Chillán, Chile, 2-5 October, 2017. Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. 520 $aABSTRACT. Olive scab caused by Venturia oleaginea is one of the most important diseases of olive worldwide. The fungus infects young leaves and fruits by asexually produced conidia. The sexual stage and its importance in the epidemiology and genetics of the pathogen population is unknown. A collection of 52 fungal isolates was obtained from scabbed leaves collected in 16 orchards from three major regions in Uruguay. All isolates were identified as V. oleaginea by PCR with specific primers and sequence analysis of the TEF-1α gene region. Five colony morphotypes and different growing rates were observed on potato dextrose agar (PDA) medium. Universally primed polymerase chain reaction (UP-PCR) was successfully used for assessing the genetic diversity and population structure of the V. oleaginea isolates, whereas ISSR was unable to generate reproducible and polymorphic bands. Two populations genetically different were identified: [1] composed by 21 isolates mainly from Southern and South-Western regions; [2] composed by 31 isolates mainly from South-Eastern and Southern regions. Both populations showed a moderate gene diversity expressed as hNei = 0.163 and 0.212 for population [1] and [2], respectively. There was evidence of linkage disequilibrium in both populations, rejecting the null hypothesis of random mating as the predominant reproduction mode, a typical result of clonal populations. However, all isolates were genotypically different, an indirect evidence of recombination. We conclude that V. oleaginea populations in Uruguay present a mixed mode of reproduction, a characteristic of many pathogens that are difficult to control. © 2019, Società Italiana di Patologia Vegetale (S.I.Pa.V.). 650 $aOLEA EUROPAEA 650 $aOLIVOS 653 $aFusicladium oleagineum 653 $aGenetic diversity 653 $aOlive scab 700 1 $aLEONI, C. 700 1 $aALANIZ, S. 773 $tJournal of Plant Pathology, 1 February 2020, Volume 102, Issue 1, Pages 123-133. Doi: https://doi.org/10.1007/s42161-019-00396-2
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INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas; INIA Tacuarembó. |
Fecha actual : |
21/02/2014 |
Actualizado : |
13/10/2015 |
Tipo de producción científica : |
Actividades de Difusión |
Autor : |
PÉREZ, J.A.; AGUERRE, V.; CARDOZO, O.; CAPRA, G. |
Afiliación : |
JUAN ANTONIO PÉREZ, Técnico Contratado. CNFR (Comisión Nacional de Fomento Rural); MARIA VERONICA AGUERRE ANTIA, Técnico Contratado. CNFR (Comisión Nacional de Fomento Rural); OSVALDO CARDOZO SARAVIA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; GUSTAVO ENRIQUE CAPRA CASSINONI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
Propuestas de producción de carne vacuna intensiva para predios de área reducida |
Fecha de publicación : |
2003 |
Fuente / Imprenta : |
Canelones (Uruguay): INIA, 2003. |
Páginas : |
24 p. |
Serie : |
(INIA Serie Actividades de Difusión ; 338) |
Idioma : |
Español |
Thesagro : |
BOVINOS PARA CARNE; CARNE; CRIANZA INTENSIVA; GANADERIA; PRODUCCION ANIMAL; PRODUCCION DE CARNE; PRODUCCION FAMILIAR; SISTEMAS DE EXPLOTACION. |
Asunto categoría : |
L01 Ganadería |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/355/1/111219210807174051.pdf
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Marc : |
LEADER 00747naa a2200265 a 4500 001 1003256 005 2015-10-13 008 2003 bl uuuu u00u1 u #d 100 1 $aPÉREZ, J.A. 245 $aPropuestas de producción de carne vacuna intensiva para predios de área reducida 260 $c2003 300 $a24 p. 490 $a(INIA Serie Actividades de Difusión ; 338) 650 $aBOVINOS PARA CARNE 650 $aCARNE 650 $aCRIANZA INTENSIVA 650 $aGANADERIA 650 $aPRODUCCION ANIMAL 650 $aPRODUCCION DE CARNE 650 $aPRODUCCION FAMILIAR 650 $aSISTEMAS DE EXPLOTACION 700 1 $aAGUERRE, V. 700 1 $aCARDOZO, O. 700 1 $aCAPRA, G. 773 $tCanelones (Uruguay): INIA, 2003.
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